T-Cell Immune Surveillance in Allogenic Stem Cell Transplant Recipients: Are Whole Blood–Based Assays Ready to Challenge ELISPOT?
Please always quote using this URN: urn:nbn:de:bvb:20-opus-363164
- We compared the feasibility of 4 cytomegalovirus (CMV)- and Aspergillus-reactive T-cell immunoassay protocols in allogenic stem cell transplant recipients. While enzyme-linked immunospot performed best overall, logistically advantageous whole blood–based assays performed comparably in patients with less severe lymphocytopenia. CMV-induced interferon-gamma responses correlated strongly across all protocols and showed high concordance with serology.
| Author: | Chris D. Lauruschkat, Lukas Page, Sonja Etter, Philipp Weis, Florian Gamon, Sabrina Kraus, Hermann Einsele, Sebastian Wurster, Juergen Loeffler |
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| URN: | urn:nbn:de:bvb:20-opus-363164 |
| Document Type: | Journal article |
| Faculties: | Medizinische Fakultät / Medizinische Klinik und Poliklinik II |
| Language: | English |
| Parent Title (English): | Open Forum Infectious Diseases |
| Year of Completion: | 2021 |
| Volume: | 8 |
| Article Number: | ofaa547 |
| Source: | Open Forum Infectious Diseases (2021) 8:ofaa547. https://doi.org/10.1093/ofid/ofaa547 |
| DOI: | https://doi.org/10.1093/ofid/ofaa547 |
| Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Tag: | ELISPOT; T cells; aspergillosis; biomarker; cytokines; cytomegalovirus; flow cytometry; immunoassay |
| Release Date: | 2024/09/05 |
| Licence (German): |  CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |