Calcium signals in guard cells enhance the efficiency by which abscisic acid triggers stomatal closure
Please always quote using this URN: urn:nbn:de:bvb:20-opus-322716
- During drought, abscisic acid (ABA) induces closure of stomata via a signaling pathway that involves the calcium (Ca2+)-independent protein kinase OST1, as well as Ca2+-dependent protein kinases. However, the interconnection between OST1 and Ca2+ signaling in ABA-induced stomatal closure has not been fully resolved.
ABA-induced Ca2+ signals were monitored in intact Arabidopsis leaves, which express the ratiometric Ca2+ reporter R-GECO1-mTurquoise and the Ca2+-dependent activation of S-type anion channels was recorded with intracellularDuring drought, abscisic acid (ABA) induces closure of stomata via a signaling pathway that involves the calcium (Ca2+)-independent protein kinase OST1, as well as Ca2+-dependent protein kinases. However, the interconnection between OST1 and Ca2+ signaling in ABA-induced stomatal closure has not been fully resolved.
ABA-induced Ca2+ signals were monitored in intact Arabidopsis leaves, which express the ratiometric Ca2+ reporter R-GECO1-mTurquoise and the Ca2+-dependent activation of S-type anion channels was recorded with intracellular double-barreled microelectrodes.
ABA triggered Ca2+ signals that occurred during the initiation period, as well as in the acceleration phase of stomatal closure. However, a subset of stomata closed in the absence of Ca2+ signals. On average, stomata closed faster if Ca2+ signals were elicited during the ABA response. Loss of OST1 prevented ABA-induced stomatal closure and repressed Ca2+ signals, whereas elevation of the cytosolic Ca2+ concentration caused a rapid activation of SLAC1 and SLAH3 anion channels.
Our data show that the majority of Ca2+ signals are evoked during the acceleration phase of stomatal closure, which is initiated by OST1. These Ca2+ signals are likely to activate Ca2+-dependent protein kinases, which enhance the activity of S-type anion channels and boost stomatal closure.…
Author: | Shouguang Huang, Rainer Waadt, Maris Nuhkat, Hannes Kollist, Rainer Hedrich, M. Rob G. Roelfsema |
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URN: | urn:nbn:de:bvb:20-opus-322716 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | New Phytologist |
Year of Completion: | 2019 |
Volume: | 224 |
Pagenumber: | 177-187 |
Source: | New Phytologist (2019) 224:177-187. https://doi.org/10.1111/nph.15985 |
DOI: | https://doi.org/10.1111/nph.15985 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Tag: | Ca2+- indicator; OST1 protein kinase; R-GECO1-mTurquoise; SLAC1 and SLAH3 anion channels; abscisic acid (ABA); cytosolic Ca2+ signals; stomata |
Release Date: | 2024/09/05 |
OpenAIRE: | OpenAIRE |
Licence (German): | ![]() |