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GABP is necessary for stem/progenitor cell maintenance and myeloid differentiation in human hematopoiesis and chronic myeloid leukemia
Please always quote using this URN: urn:nbn:de:bvb:20-opus-168165
- Maintenance of hematopoietic stem cells and their potential to give rise to progenitors of differentiated lymphoid and myeloid cells are accomplished by a network of regulatory processes. As a part of this network, the heteromeric transcription factor GA-binding protein (GABP) plays a crucial role in self-renewal of murine hematopoietic and leukemic stem cells. Here, we report the consequences of functional impairment of GABP in human hematopoietic and in leukemic stem/progenitor cells. Ectopic overexpression of a dominant-negative acting GABPMaintenance of hematopoietic stem cells and their potential to give rise to progenitors of differentiated lymphoid and myeloid cells are accomplished by a network of regulatory processes. As a part of this network, the heteromeric transcription factor GA-binding protein (GABP) plays a crucial role in self-renewal of murine hematopoietic and leukemic stem cells. Here, we report the consequences of functional impairment of GABP in human hematopoietic and in leukemic stem/progenitor cells. Ectopic overexpression of a dominant-negative acting GABP mutant led to impaired myeloid differentiation of CD34\(^{+}\) hematopoietic stem/progenitor cells obtained from healthy donors. Moreover, drastically reduced clonogenic capacity of leukemic stem/progenitor cells isolated from bone marrow aspirates of chronic myeloid leukemia (CML) patients underlines the importance of GABP on stem/progenitor cell maintenance and confirms the relevance of GABP for human myelopoiesis in healthy and diseased states.…
Author: | Georgi Manukjan, Tim Ripperger, Letizia Venturini, Michael Stadler, Gudrun Göhring, Axel Schambach, Brigitte Schlegelberger, Doris Steinemann |
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URN: | urn:nbn:de:bvb:20-opus-168165 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Experimentelle Biomedizin |
Language: | English |
Parent Title (English): | Stem Cell Research |
Year of Completion: | 2016 |
Volume: | 16 |
Issue: | 3 |
Pagenumber: | 677-681 |
Source: | Stem Cell Research 2016, 16(3), 677-681. DOI: 10.1016/j.scr.2016.04.007 |
DOI: | https://doi.org/10.1016/j.scr.2016.04.007 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | GABP; human hematopoiesis; leukemia; stem cells |
Release Date: | 2019/08/29 |
Licence (German): | CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitungen 4.0 International |