Detection of Candida albicans-Specific CD4+ and CD8+ T Cells in the Blood and Nasal Mucosa of Patients with Chronic Rhinosinusitis
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-239671
- Candida albicans is ubiquitously present, and colonization in the nose and oral cavity is common. In healthy patients, it usually does not act as a pathogen, but in some cases can cause diseases. The influence of C. albicans as a trigger of T cell activation on the pathogenesis of chronic rhinosinusitis (CRS) is controversial, and its exact role is not clear to date. The aim of the present study was to detect and characterize C. albicans-specific CD4+ and CD8+ T cells in patients with CRS, with and without nasal polyps. Tissue and blood samplesCandida albicans is ubiquitously present, and colonization in the nose and oral cavity is common. In healthy patients, it usually does not act as a pathogen, but in some cases can cause diseases. The influence of C. albicans as a trigger of T cell activation on the pathogenesis of chronic rhinosinusitis (CRS) is controversial, and its exact role is not clear to date. The aim of the present study was to detect and characterize C. albicans-specific CD4+ and CD8+ T cells in patients with CRS, with and without nasal polyps. Tissue and blood samples were collected from patients suffering from chronic rhinosinusitis with (CRSwNP) and without nasal polyps (CRSsNP), and from healthy controls. A peptide pool derived from C. albicans antigen was added to tissue and blood samples. After 6 days, lymphocytes were analyzed by multicolor flow cytometry. Activation was assessed by the intracellular marker Ki-67, and the cytokine secretion was measured. Tissue CD8+ T cells of CRSsNP patients showed a significantly higher proportion of Ki-67+ cells after activation with C. albicans antigen compared to peripheral blood CD8+ T cells. Cytokine secretion in response to C. albicans antigen was similar for all study groups. In this study, C. albicans-specific CD4+ and CD8+ T cells were detected in peripheral blood and mucosal tissue in all study groups. In patients suffering from CRSsNP, C. albicans-specific CD8+ T cells were relatively enriched in the nasal mucosa, suggesting that they might play a role in the pathogenesis of CRSsNP.…
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| Autor(en): | Pascal Ickrath, Lisa Sprügel, Niklas Beyersdorf, Agmal Scherzad, Rudolf Hagen, Stephan Hackenberg |
|---|---|
| URN: | urn:nbn:de:bvb:20-opus-239671 |
| Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
| Institute der Universität: | Medizinische Fakultät / Klinik und Poliklinik für Hals-, Nasen- und Ohrenkrankheiten, plastische und ästhetische Operationen |
| Medizinische Fakultät / Institut für Virologie und Immunbiologie | |
| Sprache der Veröffentlichung: | Englisch |
| Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Journal of Fungi |
| ISSN: | 2309-608X |
| Erscheinungsjahr: | 2021 |
| Band / Jahrgang: | 7 |
| Heft / Ausgabe: | 6 |
| Aufsatznummer: | 403 |
| Originalveröffentlichung / Quelle: | Journal of Fungi 2021, 7(6), 403; https://doi.org/10.3390/jof7060403 |
| DOI: | https://doi.org/10.3390/jof7060403 |
| Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Freie Schlagwort(e): | Candida albicans; T cell activation; chronic rhinosinusitis; nasal polyps |
| Datum der Freischaltung: | 07.01.2022 |
| Datum der Erstveröffentlichung: | 21.05.2021 |
| Open-Access-Publikationsfonds / Förderzeitraum 2021 | |
| Lizenz (Deutsch): |  CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |