Chlamydia trachomatis Infection Induces Replication of Latent HHV-6
Please always quote using this URN: urn:nbn:de:bvb:20-opus-96731
- Human herpesvirus-6 (HHV-6) exists in latent form either as a nuclear episome or integrated into human chromosomes in more than 90% of healthy individuals without causing clinical symptoms. Immunosuppression and stress conditions can reactivate HHV-6 replication, associated with clinical complications and even death. We have previously shown that co-infection of Chlamydia trachomatis and HHV-6 promotes chlamydial persistence and increases viral uptake in an in vitro cell culture model. Here we investigated C. trachomatis-induced HHV-6Human herpesvirus-6 (HHV-6) exists in latent form either as a nuclear episome or integrated into human chromosomes in more than 90% of healthy individuals without causing clinical symptoms. Immunosuppression and stress conditions can reactivate HHV-6 replication, associated with clinical complications and even death. We have previously shown that co-infection of Chlamydia trachomatis and HHV-6 promotes chlamydial persistence and increases viral uptake in an in vitro cell culture model. Here we investigated C. trachomatis-induced HHV-6 activation in cell lines and fresh blood samples from patients having Chromosomally integrated HHV-6 (CiHHV-6). We observed activation of latent HHV-6 DNA replication in CiHHV-6 cell lines and fresh blood cells without formation of viral particles. Interestingly, we detected HHV-6 DNA in blood as well as cervical swabs from C. trachomatis-infected women. Low virus titers correlated with high C. trachomatis load and vice versa, demonstrating a potentially significant interaction of these pathogens in blood cells and in the cervix of infected patients. Our data suggest a thus far underestimated interference of HHV-6 and C. trachomatis with a likely impact on the disease outcome as consequence of co-infection.…
Author: | Thomas Rudel, Bhupesh K. Prusty, Christine Siegl, Petra Hauck, Johannes Hain, Suvi J. Korhonen, Eija Hiltunen-Back, Mirja Poulakkainen |
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URN: | urn:nbn:de:bvb:20-opus-96731 |
Document Type: | Journal article |
Faculties: | Fakultät für Mathematik und Informatik / Institut für Mathematik |
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften | |
Language: | English |
Parent Title (English): | PLoS ONE |
Year of Completion: | 2013 |
Source: | In: PLoS ONE (2013) 8: 4, doi:10.1371/journal.pone.0061400 |
DOI: | https://doi.org/10.1371/journal.pone.0061400 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | DNA replication; blood; chlamydia; chlamydia infection; chlamydia trachomatis; macrophages; polymerase chain reaction; viral load |
Release Date: | 2014/04/30 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2013 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |