The glycoprotein nature of A\(_1\) adenosine receptors
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- A\(_1\) adenosine receptors from different tissues and species we~e photoaffinity labelled and then the carbohydrate content was examined by both enzymatic and chemical treatment. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the labelled membrane receptors shows that neuraminidase treatment alters the electrophoretic mobility of the receptor band indica ting the presence of terminal neurandnie acids. Neuraminidase digestion does not influence the binding characteristics of the receptor. The totally deglycosylated receptorA\(_1\) adenosine receptors from different tissues and species we~e photoaffinity labelled and then the carbohydrate content was examined by both enzymatic and chemical treatment. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the labelled membrane receptors shows that neuraminidase treatment alters the electrophoretic mobility of the receptor band indica ting the presence of terminal neurandnie acids. Neuraminidase digestion does not influence the binding characteristics of the receptor. The totally deglycosylated receptor protein obtained by chemical treatment has an apparent molecular weight Of 32,000.…
| Autor(en): | Karl-Norbert Klotz, M. J. Lohse |
|---|---|
| URN: | urn:nbn:de:bvb:20-opus-60231 |
| Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
| Institute der Universität: | Medizinische Fakultät / Institut für Pharmakologie und Toxikologie |
| Sprache der Veröffentlichung: | Englisch |
| Erscheinungsjahr: | 1986 |
| Originalveröffentlichung / Quelle: | Biochemical and Biophysical Research Communications (1986) 1, 140, 406-413. |
| Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
| Normierte Schlagworte (GND): | Toxikologie |
| Datum der Freischaltung: | 08.12.2011 |
| Lizenz (Deutsch): |  Deutsches Urheberrecht |