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Stability of rubella virus after long-term persistence in human cell line
Please always quote using this URN: urn:nbn:de:bvb:20-opus-46944
- Primary infection of HEp-2 cells with rubella virus resulted in non-cytophatic longterm persistent infection. During four years of persistence the virus was produced in sufficient quantities (up to 6 logs PFU/ml) and did not differ from the parental variant in its pathogenicity for BHK-21 or RK-13 cells, or hemagglutinating activity, but formed smaller plaques. Persistent virus preserved the original antigenicity as judged from reciprocal hemagglutination-inhibition or plaque reduction-neutralization tests with polyclonal antisera. BothPrimary infection of HEp-2 cells with rubella virus resulted in non-cytophatic longterm persistent infection. During four years of persistence the virus was produced in sufficient quantities (up to 6 logs PFU/ml) and did not differ from the parental variant in its pathogenicity for BHK-21 or RK-13 cells, or hemagglutinating activity, but formed smaller plaques. Persistent virus preserved the original antigenicity as judged from reciprocal hemagglutination-inhibition or plaque reduction-neutralization tests with polyclonal antisera. Both original and persistent rubella viruses were thermoresistant (T 56° C) and sligthly temperature-sensitive. Clonal analysis revealed presence of ts-mutants among both original and persistent virus clones with different degrees of plating efficiency at 40°/34° C. RNA fingerprinting showed only minor changes in persistent rubella virus.…
Author: | Yu S. Boriskin, R. G. Desyatskova, N. N. Bogomolova, Valentin G. Gorboulev |
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URN: | urn:nbn:de:bvb:20-opus-46944 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Anatomie und Zellbiologie |
Language: | English |
Year of Completion: | 1986 |
Source: | In: Microbiologica (1986) 9, 235 - 242 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | Rubella virus; cell culture; persistant infection |
Release Date: | 2010/07/12 |
Licence (German): | Deutsches Urheberrecht |