The long non-coding RNA PARROT is an upstream regulator of c-Myc and affects proliferation and translation
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-166519
- Long non-coding RNAs are important regulators of gene expression and signaling pathways. The expression of long ncRNAs is dysregulated in cancer and other diseases. The identification and characterization of long ncRNAs is often challenging due to their low expression level and localization to chromatin. Here, we identify a functional long ncRNA, PARROT (Proliferation Associated RNA and Regulator Of Translation) transcribed by RNA polymerase II and expressed at a relatively high level in a number of cell lines. The PARROT long ncRNA isLong non-coding RNAs are important regulators of gene expression and signaling pathways. The expression of long ncRNAs is dysregulated in cancer and other diseases. The identification and characterization of long ncRNAs is often challenging due to their low expression level and localization to chromatin. Here, we identify a functional long ncRNA, PARROT (Proliferation Associated RNA and Regulator Of Translation) transcribed by RNA polymerase II and expressed at a relatively high level in a number of cell lines. The PARROT long ncRNA is associated with proliferation in both transformed and normal cell lines. We characterize the long ncRNA PARROT as an upstream regulator of c-Myc affecting cellular proliferation and translation using RNA sequencing and mass spectrometry following depletion of the long ncRNA. PARROT is repressed during senescence of human mammary epithelial cells and overexpressed in some cancers, suggesting an important association with proliferation through regulation of c-Myc. With this study, we add to the knowledge of cytoplasmic functional long ncRNAs and extent the long ncRNA-Myc regulatory network in transformed and normal cells.…
Autor(en): | Dubravka Vučićević, Maja Gehre, Sonam Dhamija, Lennart Friis-Hansen, David Meierhofer, Sascha Sauer, Ulf Andersson Ørom |
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URN: | urn:nbn:de:bvb:20-opus-166519 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Oncotarget |
Erscheinungsjahr: | 2016 |
Band / Jahrgang: | 7 |
Heft / Ausgabe: | 23 |
Seitenangabe: | 33934-33947 |
Originalveröffentlichung / Quelle: | Oncotarget, 2016, Vol. 7, No. 23, 33934-33947. DOI: 10.18632/oncotarget.8985 |
DOI: | https://doi.org/10.18632/oncotarget.8985 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | PARROT; c-Myc; long ncRNA; upstream regulator |
Datum der Freischaltung: | 08.07.2019 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung |