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Antibacterial activity of ceramide and ceramide analogs against pathogenic Neisseria
Please always quote using this URN: urn:nbn:de:bvb:20-opus-159367
- Certain fatty acids and sphingoid bases found at mucosal surfaces are known to have antibacterial activity and are thought to play a more direct role in innate immunity against bacterial infections. Herein, we analysed the antibacterial activity of sphingolipids, including the sphingoid base sphingosine as well as short-chain C\(_{6}\) and long-chain C\(_{16}\)-ceramides and azido-functionalized ceramide analogs against pathogenic Neisseriae. Determination of the minimal inhibitory concentration (MIC) and minimal bactericidal concentrationCertain fatty acids and sphingoid bases found at mucosal surfaces are known to have antibacterial activity and are thought to play a more direct role in innate immunity against bacterial infections. Herein, we analysed the antibacterial activity of sphingolipids, including the sphingoid base sphingosine as well as short-chain C\(_{6}\) and long-chain C\(_{16}\)-ceramides and azido-functionalized ceramide analogs against pathogenic Neisseriae. Determination of the minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) demonstrated that short-chain ceramides and a ω-azido-functionalized C\(_{6}\)-ceramide were active against Neisseria meningitidis and N. gonorrhoeae, whereas they were inactive against Escherichia coli and Staphylococcus aureus. Kinetic assays showed that killing of N. meningitidis occurred within 2 h with ω–azido-C\(_{6}\)-ceramide at 1 X the MIC. Of note, at a bactericidal concentration, ω–azido-C\(_{6}\)-ceramide had no significant toxic effect on host cells. Moreover, lipid uptake and localization was studied by flow cytometry and confocal laser scanning microscopy (CLSM) and revealed a rapid uptake by bacteria within 5 min. CLSM and super-resolution fluorescence imaging by direct stochastic optical reconstruction microscopy demonstrated homogeneous distribution of ceramide analogs in the bacterial membrane. Taken together, these data demonstrate the potent bactericidal activity of sphingosine and synthetic short-chain ceramide analogs against pathogenic Neisseriae.…
Author: | Jérôme Becam, Tim WalterORCiD, Anne Burgert, Jan SchlegelORCiD, Markus Sauer, Jürgen Seibel, Alexandra Schubert-Unkmeir |
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URN: | urn:nbn:de:bvb:20-opus-159367 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Hygiene und Mikrobiologie |
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften | |
Fakultät für Chemie und Pharmazie / Institut für Organische Chemie | |
Language: | English |
Parent Title (English): | Scientific Reports |
Year of Completion: | 2017 |
Volume: | 7 |
Pagenumber: | 17627 |
Source: | Scientific Reports 7:17627 (2017). DOI: 10.1038/s41598-017-18071-w |
DOI: | https://doi.org/10.1038/s41598-017-18071-w |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 579 Mikroorganismen, Pilze, Algen |
Tag: | Neisseria; ceramide; ceramide analogs |
Release Date: | 2018/03/28 |
Collections: | Open-Access-Publikationsfonds / Förderzeitraum 2017 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |