A primary cell-based in vitro model of the human small intestine reveals host olfactomedin 4 induction in response to Salmonella Typhimurium infection
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-350451
- Infection research largely relies on classical cell culture or mouse models. Despite having delivered invaluable insights into host-pathogen interactions, both have limitations in translating mechanistic principles to human pathologies. Alternatives can be derived from modern Tissue Engineering approaches, allowing the reconstruction of functional tissue models in vitro. Here, we combined a biological extracellular matrix with primary tissue-derived enteroids to establish an in vitro model of the human small intestinal epithelium exhibiting inInfection research largely relies on classical cell culture or mouse models. Despite having delivered invaluable insights into host-pathogen interactions, both have limitations in translating mechanistic principles to human pathologies. Alternatives can be derived from modern Tissue Engineering approaches, allowing the reconstruction of functional tissue models in vitro. Here, we combined a biological extracellular matrix with primary tissue-derived enteroids to establish an in vitro model of the human small intestinal epithelium exhibiting in vivo-like characteristics. Using the foodborne pathogen Salmonella enterica serovar Typhimurium, we demonstrated the applicability of our model to enteric infection research in the human context. Infection assays coupled to spatio-temporal readouts recapitulated the established key steps of epithelial infection by this pathogen in our model. Besides, we detected the upregulation of olfactomedin 4 in infected cells, a hitherto unrecognized aspect of the host response to Salmonella infection. Together, this primary human small intestinal tissue model fills the gap between simplistic cell culture and animal models of infection, and shall prove valuable in uncovering human-specific features of host-pathogen interplay.…
Autor(en): | Thomas DäullaryORCiD, Fabian Imdahl, Oliver Dietrich, Laura Hepp, Tobias Krammer, Christina Fey, Winfried Neuhaus, Marco MetzgerORCiD, Jörg VogelORCiD, Alexander J. WestermannORCiD, Antoine-Emmanuel Saliba, Daniela ZdziebloORCiD |
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URN: | urn:nbn:de:bvb:20-opus-350451 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Molekulare Infektionsbiologie |
Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften | |
Medizinische Fakultät / Lehrstuhl für Tissue Engineering und Regenerative Medizin | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Gut Microbes |
Erscheinungsjahr: | 2023 |
Band / Jahrgang: | 15 |
Heft / Ausgabe: | 1 |
Aufsatznummer: | 2186109 |
Originalveröffentlichung / Quelle: | Gut Microbes (2023) 15:1, 2186109. DOI: 10.1080/19490976.2023.2186109 |
DOI: | https://doi.org/10.1080/19490976.2023.2186109 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | 3D tissue model; NOTCH; OLFM4; Salmonella Typhimurium; bacterial migration; bacterial virulence; biological scaffold; filamentous Salmonella Typhimurium; infection; intestinal enteroids; olfactomedin 4 |
Datum der Freischaltung: | 28.03.2024 |
Lizenz (Deutsch): | CC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell 4.0 International |