A voltage-dependent Ca\(^{2+}\) homeostat operates in the plant vacuolar membrane
Please always quote using this URN: urn:nbn:de:bvb:20-opus-259627
- Cytosolic calcium signals are evoked by a large variety of biotic and abiotic stimuli and play an important role in cellular and long distance signalling in plants. While the function of the plasma membrane in cytosolic Ca\(^{2+}\) signalling has been intensively studied, the role of the vacuolar membrane remains elusive. A newly developed vacuolar voltage clamp technique was used in combination with live-cell imaging, to study the role of the vacuolar membrane in Ca\(^{2+}\) and pH homeostasis of bulging root hair cells ofCytosolic calcium signals are evoked by a large variety of biotic and abiotic stimuli and play an important role in cellular and long distance signalling in plants. While the function of the plasma membrane in cytosolic Ca\(^{2+}\) signalling has been intensively studied, the role of the vacuolar membrane remains elusive. A newly developed vacuolar voltage clamp technique was used in combination with live-cell imaging, to study the role of the vacuolar membrane in Ca\(^{2+}\) and pH homeostasis of bulging root hair cells of Arabidopsis. Depolarisation of the vacuolar membrane caused a rapid increase in the Ca\(^{2+}\) concentration and alkalised the cytosol, while hyperpolarisation led to the opposite responses. The relationship between the vacuolar membrane potential, the cytosolic pH and Ca2+ concentration suggests that a vacuolar H\(^{+}\)/Ca\(^{2+}\) exchange mechanism plays a central role in cytosolic Ca2+ homeostasis. Mathematical modelling further suggests that the voltage-dependent vacuolar Ca\(^{2+}\) homeostat could contribute to calcium signalling when coupled to a recently discovered K\(^{+}\) channel-dependent module for electrical excitability of the vacuolar membrane.…
Author: | Julian Dindas, Ingo Dreyer, Shouguang Huang, Rainer Hedrich, M. Rob G. RoelfsemaORCiD |
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URN: | urn:nbn:de:bvb:20-opus-259627 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Julius-von-Sachs-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | New Phytologist |
Year of Completion: | 2021 |
Volume: | 230 |
Issue: | 4 |
Pagenumber: | 1449–1460 |
Source: | New Phytologist 2021, 230(4):1449–1460. DOI: 10.1111/nph.17272 |
DOI: | https://doi.org/10.1111/nph.17272 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 570 Biowissenschaften; Biologie |
Tag: | Arabidopsis thaliana; R-GECO1 cytosolic Ca\(^{2+}\) reporter; TPC1 channel; calcium signalling; computational cell biology; cpYFP cytosolic pH reporter; vacuolar membrane; voltage clamp |
Release Date: | 2022/04/06 |
Licence (German): | CC BY-NC: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell 4.0 International |