Cytogenetic changes in primary, immortalized and malignant mammalian cells
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-63439
- Some chromosomes in transformed rat cells and somatic cell hybrids fail to display the presence of kinetochore proteins as detected by antikinetochore antibodies. Suchchromosomes (K- Chromosomes) may constitute a novel mechanism for the genesis of aneuploidy. Wehave analyzed primary~ immortalized and malignant marnmalian cells for the presence of kinetochore proteins and micronuclei. Our resuJts suggest a correlation of the K- chromosome and micronucleus frequency with the variability in chromosome number. Upon in situ hybridization with theSome chromosomes in transformed rat cells and somatic cell hybrids fail to display the presence of kinetochore proteins as detected by antikinetochore antibodies. Suchchromosomes (K- Chromosomes) may constitute a novel mechanism for the genesis of aneuploidy. Wehave analyzed primary~ immortalized and malignant marnmalian cells for the presence of kinetochore proteins and micronuclei. Our resuJts suggest a correlation of the K- chromosome and micronucleus frequency with the variability in chromosome number. Upon in situ hybridization with the minor satellite and alpha satellite sequences some Kchromosomes showed a signal. This indicates that the observed lack of kinetocbores is not necessarily due to a lack of centromeric DNA. We conclude that dislocated K- chromosomes may become incorporated into micronuclei which are prone to loss. Such events would be associated with the generation of aneuploidy.…
Autor(en): | S. Kirchner, Helga Stopper, T. Papp, I. Eckert, H. J. Yoo, B. K. Vig, D. Schiffmann |
---|---|
URN: | urn:nbn:de:bvb:20-opus-63439 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Pharmakologie und Toxikologie |
Sprache der Veröffentlichung: | Englisch |
Erscheinungsjahr: | 1993 |
Originalveröffentlichung / Quelle: | In: Toxicology Letters (1993) 67(1/3) 283-295. |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Normierte Schlagworte (GND): | Toxikologie |
Freie Schlagwort(e): | Chromosome distribution; Kinetochore; Micronuclei |
Datum der Freischaltung: | 12.07.2012 |
Lizenz (Deutsch): | Deutsches Urheberrecht |