Establishment of a New Murine Elastase-Induced Aneurysm Model Combined with Transplantation
Please always quote using this URN: urn:nbn:de:bvb:20-opus-115774
- Introduction: The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation. Methods: Adult male mice (n = 6-9 per group) underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting. Results: Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared toIntroduction: The aim of our study was to develop a reproducible murine model of elastase-induced aneurysm formation combined with aortic transplantation. Methods: Adult male mice (n = 6-9 per group) underwent infrarenal, orthotopic transplantation of the aorta treated with elastase or left untreated. Subsequently, both groups of mice were monitored by ultrasound until 7 weeks after grafting. Results: Mice receiving an elastase-pretreated aorta developed aneurysms and exhibited a significantly increased diastolic vessel diameter compared to control grafted mice at 7 week after surgery (1.11 +/- 0.10 mm vs. 0.75 +/- 0.03 mm; p <= 0.001). Histopathological examination revealed disruption of medial elastin, an increase in collagen content and smooth muscle cells, and neointima formation in aneurysm grafts. Conclusions: We developed a reproducible murine model of elastase-induced aneurysm combined with aortic transplantation. This model may be suitable to investigate aneurysm-specific inflammatory processes and for use in gene-targeted animals.…
Author: | Zuzanna Rowinska, Simone Gorressen, Marc W. Merx, Thomas A. Koeppel, Elisa A. Liehn, Alma Zernecke |
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URN: | urn:nbn:de:bvb:20-opus-115774 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Klinische Biochemie und Pathobiochemie |
Language: | English |
Parent Title (English): | PLOS ONE |
ISSN: | 1932-6203 |
Year of Completion: | 2014 |
Volume: | 9 |
Issue: | 7 |
Pagenumber: | e102648 |
Source: | PLoS ONE 9(7): e102648. doi:10.1371/journal.pone.0102648 |
DOI: | https://doi.org/10.1371/journal.pone.0102648 |
Pubmed Id: | https://pubmed.ncbi.nlm.nih.gov/25068788 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | abdominal aortic-aneurysm; dilation; mouse models; prediction; rupture |
Release Date: | 2015/07/21 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung |