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A modified limiting dilution method for monoclonal stable cell line selection using a real-time fluorescence imaging system: A practical workflow and advanced applications
Please always quote using this URN: urn:nbn:de:bvb:20-opus-228896
- Stable cell lines are widely used in laboratory research and pharmaceutical industry. They are mainly applied in recombinant protein and antibody productions, gene function studies, drug screens, toxicity assessments, and for cancer therapy investigation. There are two types of cell lines, polyclonal and monoclonal origin, that differ regarding their homogeneity and heterogeneity. Generating a high-quality stable cell line, which can grow continuously and carry a stable genetic modification without alteration is very important for most studies,Stable cell lines are widely used in laboratory research and pharmaceutical industry. They are mainly applied in recombinant protein and antibody productions, gene function studies, drug screens, toxicity assessments, and for cancer therapy investigation. There are two types of cell lines, polyclonal and monoclonal origin, that differ regarding their homogeneity and heterogeneity. Generating a high-quality stable cell line, which can grow continuously and carry a stable genetic modification without alteration is very important for most studies, because polyclonal cell lines of multicellular origin can be highly variable and unstable and lead to inconclusive experimental results. The most commonly used technologies of single cell originate monoclonal stable cell isolation in laboratory are fluorescence-activated cell sorting (FACS) sorting and limiting dilution cloning. Here, we describe a modified limiting dilution method of monoclonal stable cell line selection using the real-time fluorescence imaging system IncuCyte\(^®\)S3.…
Author: | Mingyu Ye, Martina Wilhelm, Ivaylo GentschevORCiD, Aladár Szalay |
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URN: | urn:nbn:de:bvb:20-opus-228896 |
Document Type: | Journal article |
Faculties: | Fakultät für Biologie / Theodor-Boveri-Institut für Biowissenschaften |
Language: | English |
Parent Title (English): | Methods and Protocols |
Year of Completion: | 2021 |
Volume: | 4 |
Issue: | 1 |
Article Number: | 16 |
Source: | Methods and Protocols (2021), 4(1):16. DOI: 10.3390/mps4010016 |
DOI: | https://doi.org/10.3390/mps4010016 |
Dewey Decimal Classification: | 5 Naturwissenschaften und Mathematik / 57 Biowissenschaften; Biologie / 572 Biochemie |
Tag: | limiting dilution cloning; monoclonal stable cell; ncuCyte\(^®\)S3 |
Release Date: | 2021/09/10 |
Open-Access-Publikationsfonds / Förderzeitraum 2021 | |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |