Immune cells invade the collateral circulation during human stroke: prospective replication and extension
Please always quote using this URN: urn:nbn:de:bvb:20-opus-284281
- It remains unclear if principal components of the local cerebral stroke immune response can be reliably and reproducibly observed in patients with acute large-vessel-occlusion (LVO) stroke. We prospectively studied a large independent cohort of n = 318 consecutive LVO stroke patients undergoing mechanical thrombectomy during which cerebral blood samples from within the occluded anterior circulation and systemic control samples from the ipsilateral cervical internal carotid artery were obtained. An extensive protocol was applied to homogenizeIt remains unclear if principal components of the local cerebral stroke immune response can be reliably and reproducibly observed in patients with acute large-vessel-occlusion (LVO) stroke. We prospectively studied a large independent cohort of n = 318 consecutive LVO stroke patients undergoing mechanical thrombectomy during which cerebral blood samples from within the occluded anterior circulation and systemic control samples from the ipsilateral cervical internal carotid artery were obtained. An extensive protocol was applied to homogenize the patient cohort and to standardize the procedural steps of endovascular sample collection, sample processing, and laboratory analyses. N = 58 patients met all inclusion criteria. (1) Mean total leukocyte counts were significantly higher within the occluded ischemic cerebral vasculature (I) vs. intraindividual systemic controls (S): +9.6%, I: 8114/µL ± 529 vs. S: 7406/µL ± 468, p = 0.0125. (2) This increase was driven by neutrophils: +12.1%, I: 7197/µL ± 510 vs. S: 6420/µL ± 438, p = 0.0022. Leukocyte influx was associated with (3) reduced retrograde collateral flow (R\(^2\) = 0.09696, p = 0.0373) and (4) greater infarct extent (R\(^2\) = 0.08382, p = 0.032). Despite LVO, leukocytes invade the occluded territory via retrograde collateral pathways early during ischemia, likely compromising cerebral hemodynamics and tissue integrity. This inflammatory response can be reliably observed in human stroke by harvesting immune cells from the occluded cerebral vascular compartment.…
Author: | Marc Strinitz, Mirko Pham, Alexander G. März, Jörn Feick, Franziska Weidner, Marius L. Vogt, Fabian Essig, Hermann Neugebauer, Guido Stoll, Michael K. Schuhmann, Alexander M. Kollikowski |
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URN: | urn:nbn:de:bvb:20-opus-284281 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Neurologische Klinik und Poliklinik |
Medizinische Fakultät / Institut für diagnostische und interventionelle Neuroradiologie (ehem. Abteilung für Neuroradiologie) | |
Language: | English |
Parent Title (English): | International Journal of Molecular Sciences |
ISSN: | 1422-0067 |
Year of Completion: | 2021 |
Volume: | 22 |
Issue: | 17 |
Article Number: | 9161 |
Source: | International Journal of Molecular Sciences (2021) 22:17, 9161. https://doi.org/10.3390/ijms22179161 |
DOI: | https://doi.org/10.3390/ijms22179161 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Tag: | cerebral ischemia; collateral circulation; ischemic stroke; large vessel occlusion; leukocytes; mechanical thrombectomy; neutrophils |
Release Date: | 2023/06/28 |
Date of first Publication: | 2021/08/25 |
Licence (German): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |