- Treffer 1 von 1
GATA-dependent regulatory switches establish atrioventricular canal specificity during heart development
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-121437
- The embryonic vertebrate heart tube develops an atrioventricular canal that divides the atrial and ventricular chambers, forms atrioventricular conduction tissue and organizes valve development. Here we assess the transcriptional mechanism underlying this localized differentiation process. We show that atrioventricular canal-specific enhancers are GATA-binding site-dependent and act as switches that repress gene activity in the chambers. We find that atrioventricular canal-specific gene loci are enriched in H3K27ac, a marker of activeThe embryonic vertebrate heart tube develops an atrioventricular canal that divides the atrial and ventricular chambers, forms atrioventricular conduction tissue and organizes valve development. Here we assess the transcriptional mechanism underlying this localized differentiation process. We show that atrioventricular canal-specific enhancers are GATA-binding site-dependent and act as switches that repress gene activity in the chambers. We find that atrioventricular canal-specific gene loci are enriched in H3K27ac, a marker of active enhancers, in atrioventricular canal tissue and depleted in H3K27ac in chamber tissue. In the atrioventricular canal, Gata4 activates the enhancers in synergy with Bmp2/Smad signalling, leading to H3K27 acetylation. In contrast, in chambers, Gata4 cooperates with pan-cardiac Hdac1 and Hdac2 and chamber-specific Hey1 and Hey2, leading to H3K27 deacetylation and repression. We conclude that atrioventricular canal-specific enhancers are platforms integrating cardiac transcription factors, broadly active histone modification enzymes and localized co-factors to drive atrioventricular canal-specific gene activity.…
Autor(en): | Sonia Stefanovic, Phil Barnett, Karel van Duijvenboden, David Weber, Manfred Gessler, Vincent M. Christoffels |
---|---|
URN: | urn:nbn:de:bvb:20-opus-121437 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Theodor-Boveri-Institut für Biowissenschaften |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Nature Communications |
ISSN: | 2041-1723 |
Erscheinungsjahr: | 2014 |
Band / Jahrgang: | 5 |
Heft / Ausgabe: | 3680 |
Originalveröffentlichung / Quelle: | Nature Communications 5:3680 doi: 10.1038/ncomms4680 (2014). |
DOI: | https://doi.org/10.1038/ncomms4680 |
PubMed-ID: | https://pubmed.ncbi.nlm.nih.gov/24770533 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | biological sciences; developmental biology; molecular biology |
Datum der Freischaltung: | 19.02.2016 |
Lizenz (Deutsch): | CC BY-NC-ND: Creative-Commons-Lizenz: Namensnennung, Nicht kommerziell, Keine Bearbeitung |