In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-279208
- Deoxyribozymes are artificially evolved DNA molecules with catalytic abilities. RNA-cleaving deoxyribozymes have been recognized as an efficient tool for detection of modifications in target RNAs and provide an alternative to traditional and modern methods for detection of ribose or nucleobase methylation. However, there are only few examples of DNA enzymes that specifically reveal the presence of a certain type of modification, including N6-methyladenosine, and the knowledge about how DNA enzymes recognize modified RNAs is still extremelyDeoxyribozymes are artificially evolved DNA molecules with catalytic abilities. RNA-cleaving deoxyribozymes have been recognized as an efficient tool for detection of modifications in target RNAs and provide an alternative to traditional and modern methods for detection of ribose or nucleobase methylation. However, there are only few examples of DNA enzymes that specifically reveal the presence of a certain type of modification, including N6-methyladenosine, and the knowledge about how DNA enzymes recognize modified RNAs is still extremely limited. Therefore, DNA enzymes cannot be easily engineered for the analysis of desired RNA modifications, but are instead identified by in vitro selection from random DNA libraries using synthetic modified RNA substrates. This protocol describes a general in vitro selection stagtegy to evolve new RNA-cleaving DNA enzymes that can efficiently differentiate modified RNA substrates from their unmodified counterpart.…
Autor(en): | Anam Liaqat, Maksim V. Sednev, Claudia HöbartnerORCiD |
---|---|
URN: | urn:nbn:de:bvb:20-opus-279208 |
Dokumentart: | Aufsatz in einem Sammelband / Buchkapitel |
Institute der Universität: | Fakultät für Chemie und Pharmazie / Institut für Organische Chemie |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Ribosome Biogenesis: Methods and Protocols |
Erscheinungsjahr: | 2022 |
Verlag: | Humana Press |
Schriftenreihe: | Methods in Molecular Biology 2533 |
ISBN: | 978-1-0716-2501-9 |
Seitenangabe: | 167-179 |
Originalveröffentlichung / Quelle: | Liaqat, A., Sednev, M.V., Höbartner, C. (2022). In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications. In: Entian, KD. (eds) Ribosome Biogenesis. Methods in Molecular Biology, vol 2533. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2501-9_10 |
DOI: | https://doi.org/10.1007/978-1-0716-2501-9_10 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 5 Naturwissenschaften und Mathematik / 54 Chemie / 547 Organische Chemie |
Freie Schlagwort(e): | RNA; RNA cleavage; catalytic DNA; deoxyribozymes; epitranscriptomics; in vitro selection; modified RNA nucleotides |
Datum der Freischaltung: | 13.07.2022 |
EU-Projektnummer / Contract (GA) number: | 682586 |
OpenAIRE: | OpenAIRE |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |