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Treatment of focal cartilage defects in minipigs with zonal chondrocyte/mesenchymal progenitor cell constructs

Please always quote using this URN: urn:nbn:de:bvb:20-opus-285118
  • Despite advances in cartilage repair strategies, treatment of focal chondral lesions remains an important challenge to prevent osteoarthritis. Articular cartilage is organized into several layers and lack of zonal organization of current grafts is held responsible for insufficient biomechanical and biochemical quality of repair-tissue. The aim was to develop a zonal approach for cartilage regeneration to determine whether the outcome can be improved compared to a non-zonal strategy. Hydrogel-filled polycaprolactone (PCL)-constructs with aDespite advances in cartilage repair strategies, treatment of focal chondral lesions remains an important challenge to prevent osteoarthritis. Articular cartilage is organized into several layers and lack of zonal organization of current grafts is held responsible for insufficient biomechanical and biochemical quality of repair-tissue. The aim was to develop a zonal approach for cartilage regeneration to determine whether the outcome can be improved compared to a non-zonal strategy. Hydrogel-filled polycaprolactone (PCL)-constructs with a chondrocyte-seeded upper-layer deemed to induce hyaline cartilage and a mesenchymal stromal cell (MSC)-containing bottom-layer deemed to induce calcified cartilage were compared to chondrocyte-based non-zonal grafts in a minipig model. Grafts showed comparable hardness at implantation and did not cause visible signs of inflammation. After 6 months, X-ray microtomography (µCT)-analysis revealed significant bone-loss in both treatment groups compared to empty controls. PCL-enforcement and some hydrogel-remnants were retained in all defects, but most implants were pressed into the subchondral bone. Despite important heterogeneities, both treatments reached a significantly lower modified O’Driscoll-score compared to empty controls. Thus, PCL may have induced bone-erosion during joint loading and misplacement of grafts in vivo precluding adequate permanent orientation of zones compared to surrounding native cartilage.show moreshow less

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Metadaten
Author: Friederike Bothe, Anne-Kathrin Deubel, Eliane Hesse, Benedict Lotz, Jürgen Groll, Carsten Werner, Wiltrud Richter, Sebastien Hagmann
URN:urn:nbn:de:bvb:20-opus-285118
Document Type:Journal article
Faculties:Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde
Language:English
Parent Title (English):International Journal of Molecular Sciences
ISSN:1422-0067
Year of Completion:2019
Volume:20
Issue:3
Article Number:653
Source:International Journal of Molecular Sciences (2019) 20:3, 653. https://doi.org/10.3390/ijms20030653
DOI:https://doi.org/10.3390/ijms20030653
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:MSC; cartilage repair; chondrocyte; minipig; osteochondral defect; starPEG hydrogel; tissue engineering; zonal construct
Release Date:2023/06/05
Date of first Publication:2019/02/02
EU-Project number / Contract (GA) number:309962
OpenAIRE:OpenAIRE
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International