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Modulation of the in vivo covalent binding of the carcinogen benzo(a)pyrene to rat liver DNA by selective induction of microsomal and nuclear aryl hydrocarbon hydroxylase activity

Please always quote using this URN: urn:nbn:de:bvb:20-opus-80132
  • The influence of microsomal (mAHH) and nuclear (nAHH) aryl hydrocarbon hydroxylase activity on the covalent binding of t:titiated benzo(a)pyrene to rat liver DNA was evaluated in vivo. Induction ofmAHH was obtained after phenobarbitone treatment (180% of control), which increased DNA binding to 210%, but left the nAHH unchanged. mAHH and nAHH were slightly indilced with dieldrin (130% and 120%), but the binding remairred unchanged. The increasing effect of mAHlt as weil as the possibly decreasing effect of nAHH induction on the binding becameThe influence of microsomal (mAHH) and nuclear (nAHH) aryl hydrocarbon hydroxylase activity on the covalent binding of t:titiated benzo(a)pyrene to rat liver DNA was evaluated in vivo. Induction ofmAHH was obtained after phenobarbitone treatment (180% of control), which increased DNA binding to 210%, but left the nAHH unchanged. mAHH and nAHH were slightly indilced with dieldrin (130% and 120%), but the binding remairred unchanged. The increasing effect of mAHlt as weil as the possibly decreasing effect of nAHH induction on the binding became obvious when the data of 11 individual rats were used to solve the equation Binding = aX(mAHH) + bX(nAHH) + c. Multiple linear regression analysis resulted in positive values for a and c, a negative value for b, and a multiple correlation coefficient R = 0.82. An influence of other enzymes involved in the metabolism of benzo(a)pyrene cannot be excluded. The Study shows clearly that the binding of a foreign compound to DNA in vivo is not only dependent on microsomal enzyme activities but also on nuclear activities even if the latter are considerably lower than those of mic'rosomes.show moreshow less

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Metadaten
Author: A. Viviani, Werner K. Lutz
URN:urn:nbn:de:bvb:20-opus-80132
Document Type:Conference Proceeding
Faculties:Medizinische Fakultät / Institut für Anatomie und Zellbiologie
Language:English
Year of Completion:1979
Source:In: Mechanism of toxic action on some target organs : drugs and other substances ; proc. of the Europ. Soc. of Toxicology ; meeting held in Berlin (West), june 25 - 28, 1978 / Chambers, Philip L. - Berlin: Springer, 1979. - [Archives of toxicology / Supplement] ; 2; ISBN: 3-540-09305-2, p. 285-8.
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
GND Keyword:DNA
Tag:Aryl Hydrocarbon Hydroxylase; Benzo(a)pyrene; Carcinogen; DNA-Binding; Dieldrin; Enzyme; Induction; Phenobarbitone; Rats
Release Date:2013/10/21
Licence (German):License LogoDeutsches Urheberrecht