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Permissivity of the NCI-60 cancer cell lines to oncolytic Vaccinia Virus GLV-1h68

Please always quote using this URN: urn:nbn:de:bvb:20-opus-141503
  • Background: Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo. Methods: In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also testedBackground: Oncolytic viral therapy represents an alternative therapeutic strategy for the treatment of cancer. We previously described GLV-1h68, a modified Vaccinia Virus with exclusive tropism for tumor cells, and we observed a cell line-specific relationship between the ability of GLV-1h68 to replicate in vitro and its ability to colonize and eliminate tumor in vivo. Methods: In the current study we surveyed the in vitro permissivity to GLV-1h68 replication of the NCI-60 panel of cell lines. Selected cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain. In order to identify correlates of permissity to viral infection, we measured transcriptional profiles of the cell lines prior infection. Results: We observed highly heterogeneous permissivity to VACV infection amongst the cell lines. The heterogeneity of permissivity was independent of tissue with the exception of B cell derivation. Cell lines were also tested for permissivity to another Vaccinia Virus and a vesicular stomatitis virus (VSV) strain and a significant correlation was found suggesting a common permissive phenotype. While no clear transcriptional pattern could be identified as predictor of permissivity to infection, some associations were observed suggesting multifactorial basis permissivity to viral infection. Conclusions: Our findings have implications for the design of oncolytic therapies for cancer and offer insights into the nature of permissivity of tumor cells to viral infection.show moreshow less

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Metadaten
Author: Maria Libera Ascierto, Andrea Worschech, Zhiya Yu, Sharon Adams, Jennifer Reinboth, Nanhai G Chen, Zoltan Pos, Rahul Roychoudhuri, Giovanni Di Pasquale, Davide Bedognetti, Lorenzo Uccellini, Fabio Rossano, Paolo A Ascierto, David F Stroncek, Nicholas P Restifo, Ena Wang, Aladar A Szalay, Francesco M Marincola
URN:urn:nbn:de:bvb:20-opus-141503
Document Type:Journal article
Faculties:Medizinische Fakultät / Medizinische Klinik und Poliklinik I
Fakultät für Chemie und Pharmazie / Lehrstuhl für Biochemie
Language:English
Parent Title (English):BMC Cancer
Year of Completion:2011
Volume:11
Issue:451
Pagenumber:1-14
Source:BMC Cancer 2011, 11:451
DOI:https://doi.org/10.1186/1471-2407-11-451
Dewey Decimal Classification:6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit
Tag:CD9; adenovirus; gene-therapy; identification; infection; panel; receptor
Release Date:2019/01/09
Licence (German):License LogoCC BY: Creative-Commons-Lizenz: Namensnennung