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Characterization of \([^3H]\)Phenobarbital Binding to Rat Brain Membranes
Please always quote using this URN: urn:nbn:de:bvb:20-opus-127894
- The binding of \([^3H]\)phenobarbital to rat brain membranes was studied in order to determine its characteristics and specificity. The binding reaction was rapid and occurred at sites of low affinity. \((K_d = 700 μM)\) and very high density \((B_{max} = 2.7 nmoll/mg protein)\). It was unaffected by temperature changes from O°C to 95°C and was maximal at pH 5. Detergents in low concentrations markedly decreased the binding, apparently without solubilizing the binding sites. It is concluded that the binding of \([^3H]\) phenobarbital is aThe binding of \([^3H]\)phenobarbital to rat brain membranes was studied in order to determine its characteristics and specificity. The binding reaction was rapid and occurred at sites of low affinity. \((K_d = 700 μM)\) and very high density \((B_{max} = 2.7 nmoll/mg protein)\). It was unaffected by temperature changes from O°C to 95°C and was maximal at pH 5. Detergents in low concentrations markedly decreased the binding, apparently without solubilizing the binding sites. It is concluded that the binding of \([^3H]\) phenobarbital is a rather non-specific interaction with the plasma membrane.…
Author: | M. J. Lohse, K.-N. Klotz, D. Ukena, U. Schwabe |
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URN: | urn:nbn:de:bvb:20-opus-127894 |
Document Type: | Journal article |
Faculties: | Medizinische Fakultät / Institut für Pharmakologie und Toxikologie |
Language: | English |
Parent Title (English): | Neuroscience Letters |
Year of Completion: | 1984 |
Volume: | 52 |
Pagenumber: | 97-101 |
Source: | Neuroscience Letters, 52 (1984) 97-101 |
Dewey Decimal Classification: | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 615 Pharmakologie, Therapeutik |
Tag: | barbiturates; radioligand binding; rat brain membranes |
Release Date: | 2016/02/24 |
Licence (German): | Deutsches Urheberrecht |