Characterisation of worldwide Helicobacter pylori strains reveals genetic conservation and essentiality of serine protease HtrA
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-190774
- HtrA proteases and chaperones exhibit important roles in periplasmic protein quality control and stress responses. The genetic inactivation of htrA has been described for many bacterial pathogens. However, in some cases such as the gastric pathogen Helicobacter pylori, HtrA is secreted where it cleaves the tumour-suppressor E-cadherin interfering with gastric disease development, but the generation of htrA mutants is still lacking. Here, we show that the htrA gene locus is highly conserved in worldwide strains. HtrA presence was confirmed inHtrA proteases and chaperones exhibit important roles in periplasmic protein quality control and stress responses. The genetic inactivation of htrA has been described for many bacterial pathogens. However, in some cases such as the gastric pathogen Helicobacter pylori, HtrA is secreted where it cleaves the tumour-suppressor E-cadherin interfering with gastric disease development, but the generation of htrA mutants is still lacking. Here, we show that the htrA gene locus is highly conserved in worldwide strains. HtrA presence was confirmed in 992 H.pylori isolates in gastric biopsy material from infected patients. Differential RNA-sequencing (dRNA-seq) indicated that htrA is encoded in an operon with two subsequent genes, HP1020 and HP1021. Genetic mutagenesis and complementation studies revealed that HP1020 and HP1021, but not htrA, can be mutated. In addition, we demonstrate that suppression of HtrA proteolytic activity with a newly developed inhibitor is sufficient to effectively kill H.pylori, but not other bacteria. We show that Helicobacter htrA is an essential bifunctional gene with crucial intracellular and extracellular functions. Thus, we describe here the first microbe in which htrA is an indispensable gene, a situation unique in the bacterial kingdom. HtrA can therefore be considered a promising new target for anti-bacterial therapy.…
Autor(en): | Nicole Tegtmeyer, Yoshan Moodley, Yoshio Yamaoka, Sandy Ramona Pernitzsch, Vanessa Schmidt, Francisco Rivas Traverso, Thomas P. Schmidt, Roland Rad, Khay Guan Yeoh, Ho Bow, Javier Torres, Markus Gerhard, Gisbert Schneider, Silja Wessler, Steffen Backert |
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URN: | urn:nbn:de:bvb:20-opus-190774 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Experimentelle Biomedizin |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Molecular Microbiology |
Erscheinungsjahr: | 2016 |
Band / Jahrgang: | 99 |
Heft / Ausgabe: | 5 |
Seitenangabe: | 925-944 |
Originalveröffentlichung / Quelle: | Molecular Microbiology (2016) 99:5, Seiten 925-944. https://doi.org/10.1111/mmi.13276 |
DOI: | https://doi.org/10.1111/mmi.13276 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | Differential RNA-sequencing; Helicobacter pylori; cag pathogenicity island; campylobacter jejuni infection; epithelial cells |
Datum der Freischaltung: | 05.02.2021 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |