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Targeting In-Stent-Stenosis with RGD- and CXCL1-Coated Mini-Stents in Mice
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-179745
- Atherosclerotic lesions that critically narrow the artery can necessitate an angioplasty and stent implantation. Long-term therapeutic effects, however, are limited by excessive arterial remodeling. We here employed a miniaturized nitinol-stent coated with star-shaped polyethylenglycole (star-PEG), and evaluated its bio-functionalization with RGD and CXCL1 for improving in-stent stenosis after implantation into carotid arteries of mice. Nitinol foils or stents (bare metal) were coated with star-PEG, and bio-functionalized with RGD, orAtherosclerotic lesions that critically narrow the artery can necessitate an angioplasty and stent implantation. Long-term therapeutic effects, however, are limited by excessive arterial remodeling. We here employed a miniaturized nitinol-stent coated with star-shaped polyethylenglycole (star-PEG), and evaluated its bio-functionalization with RGD and CXCL1 for improving in-stent stenosis after implantation into carotid arteries of mice. Nitinol foils or stents (bare metal) were coated with star-PEG, and bio-functionalized with RGD, or RGD/CXCL1. Cell adhesion to star-PEG-coated nitinol foils was unaltered or reduced, whereas bio-functionalization with RGD but foremost RGD/CXCL1 increased adhesion of early angiogenic outgrowth cells (EOCs) and endothelial cells but not smooth muscle cells when compared with bare metal foils. Stimulation of cells with RGD/CXCL1 furthermore increased the proliferation of EOCs. In vivo, bio-functionalization with RGD/CXCL1 significantly reduced neointima formation and thrombus formation, and increased re-endothelialization in apoE\(^{-/-}\) carotid arteries compared with bare-metal nitinol stents, star-PEG-coated stents, and stents bio-functionalized with RGD only. Bio-functionalization of star-PEG-coated nitinol-stents with RGD/CXCL1 reduced in-stent neointima formation. By supporting the adhesion and proliferation of endothelial progenitor cells, RGD/CXCL1 coating of stents may help to accelerate endothelial repair after stent implantation, and thus may harbor the potential to limit the complication of in-stent restenosis in clinical approaches.…
Autor(en): | Sakine Simsekyilmaz, Elisa A. Liehn, Stefan Weinandy, Fabian Schreiber, Remco T. A. Megens, Wendy Theelen, Ralf Smeets, Stefan Jockenhövel, Thomas Gries, Martin Möller, Doris Klee, Christian Weber, Alma Zernecke |
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URN: | urn:nbn:de:bvb:20-opus-179745 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Experimentelle Biomedizin |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | PLoS ONE |
Erscheinungsjahr: | 2016 |
Band / Jahrgang: | 11 |
Heft / Ausgabe: | 5 |
Aufsatznummer: | e0155829 |
Originalveröffentlichung / Quelle: | PLoS ONE 2016, 11(5):e0155829. DOI:10.1371/journal.pone.0155829 |
DOI: | https://doi.org/10.1371/journal.pone.0155829 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | carotid arteries; coatings; endothelial cells; fluorescence microscopy; mice; polymers; stem cells; stent implantation |
Datum der Freischaltung: | 11.12.2020 |
EU-Projektnummer / Contract (GA) number: | 249929 |
OpenAIRE: | OpenAIRE |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |