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Erscheinungsjahr
- 1990 (1) (entfernen)
Dokumenttyp
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- Englisch (1)
Schlagworte
- 8-Hydroxy-deoxyguanosine (1)
- Choline deficiency (1)
- DNA (1)
- Genotoxicity (1)
- Oxygen radical (1)
- Rat liver peroxisome (1)
- Thymidine glycol (1)
- Toxikologie (1)
Institut
Male rats were treated for 2 months with 1000 ppm nafenopin in the diet or for 4 or 7 days with a choline-devoid low-methionine diet. DNA was isolated from the livers and analyzed for the presence of cis-thymidine glycol-3'-phosphate (cis-dTGp) by 32P-postlabeling and for the Ievel of 8-hydroxy-deoxyguanosine (8-0H-dG) by electrochemical detection (ECD). In no DNA sample was the Ievel of cis-dTGp above the Iimit of detection of 1 modified thymidine per 106 nucleotides. With 8-0H-dG, a background Ievel of this modification of 20 8-0H-dG per 106 nucleosides was found in liver DNA of control rats, which was not affected by either treatment. It is postulated for thymidine glycol that a potential increase was below the Iimit of detection or was rapidly repaired in vivo and that the steady-state Ievel of endogenous 8-hydroxydeoxyguanosine appears not tobe influenced by the treatments chosen.