Hydrogels with Cell Adhesion Peptide‐Decorated Channel Walls for Cell Guidance
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- A method is reported for making hollow channels within hydrogels decorated with cell‐adhesion peptides exclusively at the channel surface. Sacrificial fibers of different diameters are used to introduce channels within poly(ethylene glycol) hydrogels crosslinked with maleimide‐thiol chemistry, which are backfilled with a cysteine‐containing peptide solution which is conjugated to the lumen with good spatial efficiency. This allows for peptide patterning in only the areas of the hydrogel where they are needed when used as cell‐guides, reducingA method is reported for making hollow channels within hydrogels decorated with cell‐adhesion peptides exclusively at the channel surface. Sacrificial fibers of different diameters are used to introduce channels within poly(ethylene glycol) hydrogels crosslinked with maleimide‐thiol chemistry, which are backfilled with a cysteine‐containing peptide solution which is conjugated to the lumen with good spatial efficiency. This allows for peptide patterning in only the areas of the hydrogel where they are needed when used as cell‐guides, reducing the amount of required peptide 20‐fold when compared to bulk functionalization. The power of this approach is highlighted by successfully using these patterned hydrogels without active perfusion to guide fibroblasts and olfactory ensheathing cells—the latter having unique potential in neural repair therapies.…
Autor(en): | Shuang Wang, Mariah Sarwat, Peng Wang, Denver C. Surrao, Damien G. Harkin, James A. St John, Eleonore C. L. Bolle, Aurelien Forget, Paul D. Dalton, Tim R. Dargaville |
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URN: | urn:nbn:de:bvb:20-opus-218031 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Abteilung für Funktionswerkstoffe der Medizin und der Zahnheilkunde |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Macromolecular Rapid Communications |
Erscheinungsjahr: | 2020 |
Band / Jahrgang: | 41 |
Heft / Ausgabe: | 15 |
Aufsatznummer: | 2000295 |
Originalveröffentlichung / Quelle: | Macromolecular Rapid Communications 2020, 41(15):2000295. DOI: 10.1002/marc.202000295 |
DOI: | https://doi.org/10.1002/marc.202000295 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | 3D printing; cell guidance; cell transplantation; melt electrowriting; synthetic hydrogels |
Datum der Freischaltung: | 19.08.2021 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |