Institut für Virologie und Immunbiologie
Refine
Has Fulltext
- yes (42)
Is part of the Bibliography
- yes (42)
Year of publication
Document Type
- Journal article (39)
- Conference Proceeding (3)
Keywords
- Virologie (24)
- HIV (8)
- Spumaviren (2)
- AIDS (1)
- Affenimmundefizienzvirus (1)
- DNA sequence (1)
- Einfluss (1)
- Evaluation (1)
- Fanconi Anemia (1)
- Foamy virus (1)
Institute
Sonstige beteiligte Institutionen
EU-Project number / Contract (GA) number
- 260862 (1)
During molecular cloning of proviral DNA of human. spumaretroVirus, various recombinant clones were estabUshed and analyzed. Blot hybridization revealed that one of the recoinbinant plasmids bad the characteristic features of a member of the long interspersed repetitive sequences famlly. The DNA element was analyzed by restrictioil mapping and nuelootide sequencing. It showed a high degree of amino acid sequence homology of 54.3% when conipared with the 5'-terminal part of the pol gelie product of the murine retrotransposon LIMd. The 3' region of the cloned DNA element encodes proteins witb an even higher degree of homology of 67.4% in comparison to the corresponding parts of a member of the primate Kpnl sequence family.
A virus derived from cells of a Iymphoblastoid line originating from the lymph node of a healthy African green monkey was characterized as a typical member of the foamy virus subgroup of rctroviridac by its morphological, physicochemical, biological and biochemical properties (reverse transcriptase actvity). Besides the usual host range of foamy viruses, the isolated strain revealed a remarkable T -lymphotropism, distinguishing it from the prototypes of foamy viruses previously isolated from African green monkeys. Two foamy virus infectious are demonstrated in human contacts of the African green monkey colony, with the animal barbauring the isolate.