Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I)
Refine
Has Fulltext
- yes (503)
Is part of the Bibliography
- yes (503) (remove)
Year of publication
Document Type
- Doctoral Thesis (256)
- Journal article (231)
- Conference Proceeding (12)
- Other (2)
- Habilitation (1)
- Master Thesis (1)
Keywords
- Chirurgie (30)
- obesity (11)
- cytokines (10)
- Dickdarmkrebs (9)
- Medizin (9)
- Transplantation (9)
- inflammation (9)
- Immunbiologie (8)
- Langerhans-Inseln (8)
- colorectal cancer (8)
- Lebensqualität (7)
- Rezidiv (7)
- Sepsis (7)
- sepsis (7)
- therapy (7)
- Colonkrebs (6)
- Diabetes mellitus (6)
- Kolorektales Karzinom (6)
- Periphere arterielle Verschlusskrankheit (6)
- bariatric surgery (6)
- incisional hernia (6)
- surgery (6)
- transplantation (6)
- Adipositas (5)
- Diabetes (5)
- Gefäßprothese (5)
- Hernie (5)
- Immunobiologie (5)
- Kolonkarzinom (5)
- Magenbypass (5)
- Narbenhernie (5)
- Oxidativer Stress (5)
- Ratte (5)
- inflammatory bowel disease (5)
- intestinal epithelial barrier (5)
- pancreatic cancer (5)
- quality of life (5)
- Adenokarzinom (4)
- Antikörper (4)
- Crohn-Krankheit (4)
- Fettsucht (4)
- Hitzeschock-Proteine (4)
- Immunsuppression (4)
- Infektion (4)
- Insulin (4)
- Krebs <Medizin> (4)
- Laparoskopie (4)
- Leberresektion (4)
- Makrophage (4)
- Nebenschilddrüse (4)
- Neuromonitoring (4)
- Roux-en-Y gastric bypass surgery (4)
- Therapie (4)
- Zytokine (4)
- colorectal carcinoma (4)
- hernia (4)
- immunosuppression (4)
- ischemia (4)
- rat (4)
- Anastomose (3)
- Bariatrische Chirurgie (3)
- Bauchfellentzündung (3)
- Bauchspeicheldrüse (3)
- Bauchspeicheldrüsenkrebs (3)
- Bauchwandhernie (3)
- Cancer (3)
- Crohn's disease (3)
- Differenzierung (3)
- Dünndarmtransplantation (3)
- Endothel (3)
- Immunmodulation (3)
- Kinderchirurgie (3)
- Klassifikation (3)
- Kompressionstherapie (3)
- Krebs (3)
- Lebermetastase (3)
- MYC (3)
- Mikroverkapselung (3)
- Nabelhernie (3)
- Pankreaskarzinom (3)
- Peritonitis (3)
- RYGB (3)
- Recurrensparese (3)
- Resektion (3)
- Schilddrüse (3)
- Schwein (3)
- Simulation (3)
- Toll-like-Rezeptoren (3)
- Umbilikalhernie (3)
- Ureaplasma parvum (3)
- Viszeralchirurgie (3)
- Wundheilung (3)
- Xenotransplantation (3)
- adenocarcinoma (3)
- anastomotic leakage (3)
- animal model (3)
- bridging (3)
- cancer (3)
- chemotherapy (3)
- children (3)
- colon cancer (3)
- complications (3)
- evidence-based medicine (3)
- follow-up (3)
- gastric bypass (3)
- guidelines (3)
- indication for surgery (3)
- infection (3)
- laparoscopic ventral hernia repair (3)
- liraglutide (3)
- mortality (3)
- open abdomen (3)
- outcome (3)
- oxidative stress (3)
- parastomal hernia (3)
- parathyroid (3)
- perioperative management (3)
- peritonitis (3)
- primary hyperparathyroidism (3)
- prognostic factors (3)
- rectal cancer (3)
- recurrence (3)
- regulatorische T-Zellen (3)
- regulatory T cells (3)
- results (3)
- treatment (3)
- umbilical hernia (3)
- 2 (2)
- 3-DPG (2)
- 5-Fluorouracil (2)
- ATP (2)
- Allograft rejection (2)
- Analfistel (2)
- Anastomosenheilung (2)
- Antibiotika (2)
- Antibiotikum (2)
- Aortenaneurysma (2)
- Apoptose (2)
- Arterielle Verschlusskrankheit (2)
- Aspergillus (2)
- Avemar (2)
- Barrett-Ösophagus (2)
- Bauchwand (2)
- Benzochinone (2)
- Beschneidung (2)
- Bridging (2)
- CD4+ T cells (2)
- COVID-19 (2)
- CRC (2)
- Caco2 cells (2)
- Cadherine (2)
- Carcino-embryonales Antigen (2)
- Chemotherapieresistenz (2)
- Classification (2)
- Colorectal carcinoma (2)
- DNA damage (2)
- Dacron (2)
- Darmkrebs (2)
- Druckmessung (2)
- Duodenopankreatektomie (2)
- Durchflusscytometrie (2)
- Ellenbogen (2)
- Endobrachyösophagus (2)
- Endosonographie (2)
- Endothelbarriere (2)
- Ergebnisse (2)
- Erythrozyten (2)
- Fn14 (2)
- Fundoplikatio (2)
- GDNF (2)
- Gastric-banding (2)
- Gastrointestinaler Tumor (2)
- Gastroschisis (2)
- Gastroösophageale Refluxkrankheit (2)
- Gastroösophagealer Reflux (2)
- Gefäßchirurgie (2)
- Germany (2)
- Hepatozelluläres Karzinom (2)
- Hernia (2)
- Heterotransplantation (2)
- Hodenhochstand (2)
- Hypertherme Chemotherapie (2)
- Ileum-Conduit (2)
- Immuncytochemie (2)
- In-situ-Venenbypass (2)
- Inkontinenz (2)
- Insuffizienz (2)
- Intimahyperplasie (2)
- Inzisionalhernie (2)
- Ischämie (2)
- Kind (2)
- Kinder (2)
- Kollagenase (2)
- Komplikationen (2)
- Kontinenz (2)
- LPS (2)
- Langerhansinseln (2)
- Leberzellkrebs (2)
- Leistenhernie (2)
- Lymphadenektomie (2)
- MHC (2)
- MHC Klasse II (2)
- MRSA (2)
- Magenchirurgie (2)
- Modifizierte Sugarbaker-Technik (2)
- Monozyt (2)
- Monozyten (2)
- Morbus Crohn (2)
- NAFLD (2)
- NFκB (2)
- Nachuntersuchung (2)
- Naht (2)
- Nahtmaterial (2)
- Nebenschilddrüsen (2)
- Nervus recurrens (2)
- Netz (2)
- Nierentransplantation (2)
- Nierenzellkarzinom (2)
- Nissen (2)
- Omphalocele (2)
- Onkologie (2)
- Operation (2)
- Organtransplantation (2)
- Parastomale Hernie (2)
- Pathogenese (2)
- Pauli procedure (2)
- Pauli-Verfahren (2)
- Perifusion (2)
- Peritonealkarzinose (2)
- Phimose (2)
- Port (2)
- Prognose (2)
- Prognosefaktoren (2)
- Quality of life (2)
- Rectal cancer (2)
- Refluxkrankheit (2)
- Regulatory T cells (2)
- Rektumresektion (2)
- Rezidivrate (2)
- Risikofaktor (2)
- Robotik (2)
- Roux-en-Y (2)
- SARS-CoV-2 (2)
- SIRS (2)
- Schilddrüsenoperation (2)
- Schlussleistenkomplex (2)
- Silicone (2)
- Small bowel transplantation (2)
- Speiseröhre (2)
- Stammzelle (2)
- Sublay (2)
- Surgery (2)
- T-Lymphozyten-Rezeptor (2)
- TLR7 (2)
- TLR8 (2)
- TWEAK (2)
- Tiermodell (2)
- Tissue Engineering (2)
- Toleranz (2)
- Toleranzinduktion (2)
- Transplantatabstoßung (2)
- Transplantationsimmunologie (2)
- Trauma (2)
- Trichternetz (IPST) (2)
- Ulcus cruris (2)
- Ureaplasma urealyticum (2)
- VE-Cadherin (2)
- Vorhaut (2)
- Weizenkeim (2)
- Zellkultur (2)
- Zwerchfellbruch (2)
- abdominal trauma (2)
- adaptive immunity (2)
- adrenocortical carcinoma (2)
- agonistic antibodies (2)
- anastomosis (2)
- antibiotics (2)
- anticoagulation (2)
- antimicrobial stewardship (2)
- aortic aneurysm (2)
- apoptosis (2)
- appendicitis (2)
- atrial fibrillation (2)
- autophagy (2)
- benzoquinones (2)
- beta cells (2)
- biomarker (2)
- blood (2)
- case report (2)
- cell death (2)
- childhood (2)
- chronic rejection (2)
- colon (2)
- complication (2)
- compression therapy (2)
- deconstruction into key steps (2)
- definition (2)
- dendritic cells (2)
- desmosome (2)
- diabetes (2)
- diagnosis (2)
- endoluminal (2)
- endothelial barrier (2)
- enzyme activation (2)
- enzyme inhibitors (2)
- epidemiology (2)
- extracorporeal membrane oxygenation (2)
- flow cytometry (2)
- fundoplication (2)
- funnel mesh (IPST) (2)
- gastric cancer (2)
- giant ventral hernia (2)
- gut barrier (2)
- hepatic resection (2)
- hepatocellular carcinoma (2)
- hiatal hernia (2)
- ileocecal resection (2)
- immunoassay (2)
- immunomodulation (2)
- incontinence (2)
- induction of tolerance (2)
- inguinal hernia (2)
- insulin (2)
- islets of Langerhans (2)
- kidney transplantation (2)
- kolorektales Karzinom (2)
- laparoscopic course (2)
- laparoscopic skills (2)
- laparostomy (2)
- leg ulcer (2)
- linea alba (2)
- macrophages (2)
- malignant melanoma (2)
- meta-analysis (2)
- metabolism (2)
- metastasis (2)
- microbial spectrum (2)
- modified Sugarbaker technique (2)
- monocyte (2)
- monocytes (2)
- negative pressure (2)
- neuromonitoring (2)
- orale Antikoagulation (2)
- oxaliplatin (2)
- p53 (2)
- pediatric (2)
- peptide tyrosine tyrosine (PYY) (2)
- peritoneal carcinomatosis (2)
- phosphodiesterase (2)
- postoperative Blutung (2)
- postoperative bleeding (2)
- preterm infants (2)
- primary ventral hernia (2)
- primär ventrale Hernie (2)
- prognosis (2)
- rate of recurrence (2)
- rectal resection (2)
- rectum (2)
- recurrent nerve palsy (2)
- reflux disease (2)
- regulatory t-cells (2)
- retrospective study (2)
- risk factors (2)
- sacral nerve stimulation (2)
- score (2)
- shock (2)
- skin (2)
- small bowel transplantation (2)
- surgical therapy (2)
- survival (2)
- survival rate (2)
- swine (2)
- synthetic mesh (2)
- teaching methods (2)
- terminal bar (2)
- thromboembolism (2)
- thyroid gland (2)
- tissue engineering (2)
- tolerance (2)
- translational research (2)
- vacuum conditioning (2)
- vacuum-assisted closure (2)
- vascular (2)
- ventral hernia (2)
- Überleben (2)
- 15-Deoxyspergualin (1)
- 1st-line treatment (1)
- 2,6-Dimethoxybenzochinon (1)
- 2,6-Dimethoxybenzoquinone (1)
- 5-FU (1)
- 5-fluorouracil (1)
- 6-percent hydroxyethyl starch (1)
- AAA (1)
- ABC-Transporter (1)
- ALCL (1)
- ARM (1)
- AVEMAR (1)
- Abdominaltrauma (1)
- Abstoßung (1)
- Abszess (1)
- Abszesse (1)
- Achalasie (1)
- Achilles tendo (1)
- Achillessehne (1)
- Activation (1)
- Acute Limb Ischemia (1)
- Adenocarcinom (1)
- Adrenerge Stimu (1)
- Adrenocortical Carcinoma (1)
- Aktivierung <Physiologie> (1)
- Akute Extremitätenischämie (1)
- Allergie (1)
- Allgemeine Entzündungsreaktion (1)
- Alloantigen (1)
- Alloantigenerkennung (1)
- Allopeptidb (1)
- Allotransplantatabstossung (1)
- Allotransplantation (1)
- Alpha Fetoprotein (1)
- Alpha-Lipoic acid (1)
- Alternative Medizin (1)
- Alveolar (1)
- Alveolarmakrophagen (1)
- Amputation (1)
- Analabszess (1)
- Analatresie (1)
- Anastomosendurchblutung (1)
- Anastomoseninsuffizienz (1)
- Anastomosenrezidiv (1)
- Anastomosis healing (1)
- Anastomotic leaks (1)
- Aneurysma (1)
- Angiogenese (1)
- Angiologie (1)
- Antibiose (1)
- Antibiotic Stewardship (1)
- Antigen (1)
- Antigen CD14 (1)
- Antigen CD25 (1)
- Antigen CD4 (1)
- Antigen CD44 (1)
- Antigen CD45 (1)
- Antikoerper (1)
- Antioxidans (1)
- Antirefluxchirugie (1)
- Antirefluxoperation (1)
- Aorta (1)
- Aortenbifurkationssyndrom (1)
- Apherese (1)
- Apoptosis (1)
- Appendizitis (1)
- Arteriosclerosis (1)
- Arteriosklerose (1)
- Arthroskopie (1)
- Aspiration (1)
- Aspirationspneumonie (1)
- Atresie (1)
- Attentional performance (1)
- Ausbildungsforschung (1)
- Autologer In-Situ-Venenbypass (1)
- Autologous (1)
- Autophagie (1)
- Autostimulation (1)
- Axilla (1)
- B-Zel (1)
- B7DC (1)
- B7H1 (1)
- BALB/c Maus (1)
- BAR (1)
- BM (1)
- BMP (1)
- Bakterielle Infektion (1)
- Bakterien (1)
- Bandspannung (1)
- Bariatric surgery (1)
- Bariatric/metabolic surgery (1)
- Barrett's esophagus (1)
- Barrett-Karzinom (1)
- Barrettösophagus (1)
- Basale Stimulation (1)
- Basisfertigkeiten Laparoskopie (1)
- Bauchdeckenverschluss (1)
- Bauchspalte (1)
- Bauchspeicheldruesenkrebs (1)
- Bauchwanddefekt (1)
- Bauspeicheldrüse (1)
- Beckenbodendysfunktion (1)
- Behandlungsmethoden (1)
- Behandlungsverfahren (1)
- Beinachse (1)
- Beinamputation (1)
- Beta-catenin (1)
- Bewertungsplattform (1)
- Biofeedback (1)
- Biofeedback-Therapie (1)
- Biofilm (1)
- Biomaterial (1)
- Biseko (1)
- Blasenaugmentation (1)
- Blinddarmentzündung (1)
- Blood–brain barrier (1)
- Blut (1)
- Bougierung (1)
- Brain μ-opioid receptors (1)
- Breischluckuntersuchung (1)
- Brustimplantate (1)
- Bupivacain (1)
- Bypassoperation (1)
- Bypassverschluss (1)
- C-MYC (1)
- C-Peptid (1)
- CA 19-9 (1)
- CA19-9 (1)
- CASP (1)
- CASPAR (1)
- CCR7 (1)
- CD 4+ T-Lymphozyten (1)
- CD137 (1)
- CD137-Antigen (1)
- CD137L (1)
- CD24 (1)
- CD4+ T-Lymphozyten (1)
- CD44 (1)
- CD45RC (1)
- CD62L (1)
- CD8 (1)
- CD8+ T cells (1)
- CD8-Lymphoyzten (1)
- CDH (1)
- CEA (1)
- CED (1)
- CFDASE (1)
- CIP2A (1)
- CK-20 (1)
- CNS integrity (1)
- COVID-19 pandemic (1)
- CPR-Prothese (1)
- CPR-prothesis (1)
- CT angiography (1)
- CX-5461 (1)
- CX3CL1 (1)
- CX5461 (1)
- CXCL13 (1)
- Ca-antagonisten (1)
- Cancer immunotherapy (1)
- Capillarization (1)
- Carcinoma cells (1)
- Carcinomatosis (1)
- Catgut (1)
- Cellculture (1)
- Central venous access (1)
- Central venous-pressure (1)
- ChIPseq (1)
- Chemoperfusion (1)
- Chemotherapeutic resistance (1)
- Chemotherapeutikum (1)
- Chemotherapie (1)
- Children (1)
- Chimärismus (1)
- Chinonderivate (1)
- Chirurgische Ausbildung (1)
- Cholangiocarcinoma (1)
- Cholezystektomie (1)
- Chondrozyten (1)
- Chronic rejection (1)
- Chronic wound (1)
- Chronische Abstoßung (1)
- Chronische Darmentzündung (1)
- Chronische Wunde (1)
- Class II antigen blockade (1)
- Claudicatio intermittens (1)
- Claudin2 (1)
- Clinical capillaroscopy (1)
- Cognitive (1)
- Colorectal liver metastases (1)
- Colorimetrie (1)
- Compression Injury (1)
- Compression injury (1)
- Compression therapy (1)
- Congenital abdominal wall defects (1)
- Coronary artery bypass graft (1)
- Coronavirus Disease 2019 (1)
- Critically-ill patients (1)
- Crohn’s Disease (1)
- Crohn’s disease (1)
- Cryolesion (1)
- Cryopreservation (1)
- Cryptorchidism (1)
- Cushing (1)
- Cyclo-AMP (1)
- Cyclophosphamid (1)
- Cyclosporin (1)
- Cytoplasmatische Therapie (1)
- Cytoprotektion (1)
- Cytoreductive surgery (1)
- DNA repair (1)
- DW-MRI (1)
- Damage control surgery (1)
- Danish hernia database (1)
- Darmanastomose (1)
- Darmchirurgie (1)
- Darmdekontamination (1)
- Darminkontinenz (1)
- Darmvorbereitung (1)
- Data Warehouse (1)
- Definition (1)
- Defäkographie (1)
- Dekubitalulkus (1)
- Dendritische Zelle (1)
- Diabetes mellitus Extracellular matrix Polyelectrolytes (1)
- Diabetic foot (1)
- Diabetisches Fußsyndrom (1)
- Diagnosesicherheit (1)
- Diagnostik (1)
- Dialyse (1)
- Dialysis (1)
- Dichlorvinylcystein (1)
- Dichtegradient (1)
- Dichtegradientenzentrifugation (1)
- Dichtheit (1)
- Differentiation (1)
- Diffusions-MRT (1)
- Diffusionsgewichtete Magnetresonanztomographie (1)
- Distale Radiusfraktur (1)
- Distale Radiusfrakturen (1)
- Druckoptimum (1)
- Druckschädigung (1)
- Druckwerte (1)
- Drug releasing graft (1)
- Dsg2 (1)
- Duodenalatresie (1)
- Durchflußzytometrie (1)
- Dünndarm (1)
- Dünndarm-Transplantation . Mikrochirurgie (1)
- E-Vac (1)
- EDS (1)
- EHS classification (1)
- ENPWT (1)
- ERK (1)
- ESIN (1)
- EVAC (1)
- Effizienz (1)
- Ehlers-Danlos syndrome (1)
- Eierstocktumor (1)
- Elective cesarean-section (1)
- Electrical impedance tomography (1)
- End-zu-End-Anastomose (1)
- End-zu-End-Anastomosierung (1)
- Endo-vacuum (1)
- EndoVAC and small bowel (1)
- Endoluminal vacuum (1)
- Endorectal Ultrasound (1)
- Endoscopic closure (1)
- Endoskopische Vakuumtherapie (1)
- Endotoxin (1)
- Endotoxinämie (1)
- Endotoxämie (1)
- Endovascular Therapie (1)
- Endovaskuläre Intervention (1)
- Enteroide (1)
- Entzündung (1)
- Enzyme-linked immunosorbent assay (1)
- Epidemiologie (1)
- Epithel (1)
- Epithelgewebe (1)
- Epithelkörperchen (1)
- Epstein-Barr virus (1)
- Ergebnis (1)
- Ersatzblase (1)
- Erythrozytenkonzentrat (1)
- Esophageal leakage (1)
- EuraHS (1)
- Exostose (1)
- Expansion (1)
- Experimental brain trauma (1)
- Expression (1)
- Extra-anatomical (1)
- Extrazellulärvesikel (1)
- FK 506 (1)
- FWGE (1)
- Fascia transversalis (1)
- Fast Track (1)
- Fatty Triangle (1)
- FcγR (1)
- Femoral vein (1)
- Fermentierte Weizenkeimlinge (1)
- Fetoprotein <alpha-> (1)
- Fibrin gel Alginat (1)
- Fibroblasts (1)
- Fluorouracil (1)
- Fontaine Stadien (1)
- Fontaine classification (1)
- Forearm fracture (1)
- Foxp3 (1)
- Fractures of the ankle joint (1)
- Fragebogen (1)
- Frailty (1)
- Fraktur (1)
- Frakturen (1)
- Fundoplicatio (1)
- Funktion (1)
- Fußamputation (1)
- GDNF5 (1)
- GERD (1)
- GIST (1)
- GITR (1)
- GLP-1 (1)
- GMS (1)
- Gallengangskarzinom (1)
- Gastroenterologische Endoskopie (1)
- Gastroesophageal reflux disease (1)
- Gastrointestinaler Lebensqualitätsindex nach Eypasch (GIQLI) (1)
- Gebrechlichkeit (1)
- Gedächtniszellen (1)
- Gefäßanastomose (1)
- Gefäßersatz (1)
- Gefäßprothesen (1)
- Gefäßprotheseninfektion (1)
- Gefäßresektion (1)
- Gefäßverletzung (1)
- General anaesthesia (1)
- Genexpression (1)
- Gerinnbarkeit (1)
- Geruchsmpfinden (1)
- Gewaschene Erythrozytenkonzentrate (1)
- Gewichtsverlust (1)
- Gist (1)
- Glia (1)
- Glucocorticosteroide (1)
- Glutaminstoffwechsel (1)
- Gore-Tex (1)
- Groin infection (1)
- Growth-factor receptor (1)
- Göttingen (1)
- HAI (1)
- HBMEC (1)
- HCC (1)
- HIPEC (1)
- HWI (1)
- Handgelenk (1)
- Handgelenksarthroskopie (1)
- Handnaht (1)
- Harnableitung (1)
- Harnwegsinfektion (1)
- Harris Hip Score (1)
- Heat shock protein (1)
- Hepatischer Ischämie-Reperfusionsschaden (1)
- Hepatozyten-Wachstumsfaktor (1)
- Hernienchirurgie (1)
- Herniengesellschaft (1)
- Hiatushernie (1)
- Hickman catheter (1)
- Hickman-Broviac-Katheter (1)
- High-fat diet (1)
- Hirntod (1)
- Histamin H1 Rezeptor (1)
- Histamine H1 receptor (1)
- Histokompatibilität (1)
- Histologie (1)
- Hormonsubstitution (1)
- Human Leukocyte antigen (1)
- Human-Leukozyten-Antigen (1)
- Hybrideingriffe (1)
- Hydatide (1)
- Hygiene (1)
- Hyperparathyreoidismus (1)
- Hypertonic saline 7.5-percent (1)
- Hypertonie (1)
- Hypertrophe Narbe (1)
- Hypocalcämie (1)
- Hypoparathyreoidismus (1)
- Hüftgelenkarthrose (1)
- Hüfttotalendoprothese (1)
- ICD-10 (1)
- ICU (1)
- IDO (1)
- IL-10 (1)
- IL-13 (1)
- IL-6 (1)
- IPOM (1)
- IVF-methods (1)
- Ileal conduit (1)
- Ileitis terminalis (1)
- Ileostomarückverlagerung (1)
- Ileozökalresektion (1)
- Immun-Checkpoint (1)
- Immunantwort (1)
- Immunglobulin (1)
- Immunhistologie (1)
- Immunologie (1)
- Immunomodulation (1)
- Immunosenescence (1)
- Immunotherapy (1)
- Immunreaktion (1)
- Immunregulation (1)
- Immuntherapie (1)
- Immuntoleranz (1)
- Implantation (1)
- Improved survival (1)
- In vitro models (1)
- In-Vitro-Untersuchungen (1)
- Incisional hernia (1)
- Indolamin-2,3-Dioxygenase (1)
- Indoleamine-2,3-dioxygenase (1)
- Infektiösität (1)
- Inhibition (1)
- Inhibitor of Growth (1)
- Injektion (1)
- Inkompetenz unterer ösophagealer Sphinkter (1)
- Inselzellen (1)
- Inselzelltransplantation (1)
- Insulinsensitivität (1)
- Insulinstoffwechsel (1)
- Intensivstation (1)
- Interferon (1)
- Interleukine (1)
- International Conference on Tumor Microenvironment (4 : 2007 : Florenz) (1)
- Intervascular (1)
- Intestinale Epithelbarriere (1)
- Intestinale Metaplasie (1)
- Intimal hyperplasia (1)
- Intraabdominelle Infektion (1)
- Intravenös (1)
- Invasion (1)
- Inzidenz (1)
- Inzisionale Hernie (1)
- IsaA (1)
- IsaB (1)
- Islets of Langerhans (1)
- Isolierung <Mikrobiologie> (1)
- J-Pouch (1)
- JQ1 (1)
- Juvenile Knochenzyste (1)
- K-wire (1)
- KIT (1)
- Kanzerogenese (1)
- Kapillarisierung (1)
- Kapillarmikroskopie (1)
- Karzinomzellen (1)
- Keimspektrum (1)
- Keimzelltumor (1)
- Keloide (1)
- Kernspintomografie (1)
- Ketonkörper (1)
- Kienboeck's disease (1)
- Kienböck (1)
- Kinderheilkunde (1)
- Kindertraumatologie (1)
- Kindesalter (1)
- Kindliche Schaftfraktur (1)
- Kirschner-Draht (1)
- Knieprothese (1)
- Knochentumor (1)
- Kolon (1)
- Kolorektale Lebermetastasen (1)
- Koloskopie (1)
- Kombinationsbehandlung (1)
- Kommunikation (1)
- Komorbidität (1)
- Komplikationsmanagement (1)
- Kongenitale Bauchwanddefekte (1)
- Kono-S anastomosis (1)
- Konstruktvalidität (1)
- Kontrollierte klinische Studie (1)
- Kortisoninstillation (1)
- Kosten (1)
- Kosten-Wirksamkeits-Analyse (1)
- Kostimulatorische Signalwege (1)
- Kritische Extremitätenischämie (1)
- Kruraler Bypass (1)
- Kryokonservierung (1)
- Kryptorchismus (1)
- Kunststoffbypass (1)
- LAMN (1)
- LND (1)
- LNE (1)
- Lactate (1)
- Lactated ringers solution (1)
- Langenbecks Archiv für Chirurgie (1)
- Langerhans (1)
- Langerhans Inseln (1)
- Langzeiteffektivität (1)
- Langzeitergebnisse (1)
- Laparoscopic adjustable Gastric Banding (1)
- Laparoscopic adjustable gastric banding (1)
- Laparoscopy (1)
- Laparoskopietraining (1)
- Laparoskopische Cholecystektomie (1)
- Laparoskopische Fundoplikatio (1)
- Laparoskopische Heller Myotomie (LHM) (1)
- Laparostomie (1)
- Laparotomie (1)
- Latenzzeit (1)
- Leber/Dünndarmtransplantation (1)
- Leberchirurgie (1)
- Leberfunktion (1)
- Leberfunktionsevaluation (1)
- Leberfunktionsreserve (1)
- Leberkrebs (1)
- Lebermetastasen (1)
- Lebertransplantat-Spontantoleranz (1)
- Lebertransplantation (1)
- Lebertumor (1)
- Leberversagen (1)
- Leckage (1)
- Lehrmethoden (1)
- Leiste (1)
- Leitlinie (1)
- Lendenwirbelsäule (1)
- Leptin (1)
- Lernkurve (1)
- Lerntechnik (1)
- LiMAx-Test (1)
- Linea alba (1)
- Lipopolysaccharid (1)
- Liver metastasis (1)
- Lokalisation (1)
- Lokaltherapie (1)
- Lokoregionäre Hyperthermie (1)
- Long-term efficiency (1)
- Low-fat diet (1)
- Lunatumnekrose (1)
- Lung metastasis (1)
- Lunge (1)
- Lungenentzündung (1)
- Lungenmetastase (1)
- LyP (1)
- Lymph nodes (1)
- Lymphfistel (1)
- Lymphozele (1)
- Lyoplant (1)
- MAPK pathway (1)
- MAPK signaling cascades (1)
- MCS+ cell separator (1)
- MHC I (1)
- MHC II peptides (1)
- MHC class II (1)
- MHC class II molecules (1)
- MHC class II-positive T cells (1)
- MHC molecules (1)
- MHC-II Peptide (1)
- MHC-Klass I (1)
- MHC-Klasse II Moleküle (1)
- MHC-Klasse-II positive T-Lymphozyten (1)
- MITF (1)
- MIZ1 (1)
- MMP-9 (1)
- MPFL (1)
- MRI (1)
- MRT (1)
- MRT Diffusionswichtung (1)
- MTL30 (1)
- Macrophage (1)
- Magenballon (1)
- Magenbandoperation (1)
- Major abdominal surgery (1)
- Makrophagen (1)
- Maldescensus Testis (1)
- Maldescensus testis (1)
- Malnutration (1)
- Mammalian target (1)
- Mammographie (1)
- Mastdarmchirurgie (1)
- Mastdarmkrebs (1)
- Matrix (1)
- Maus (1)
- Medikamente (1)
- Medizinische Lehre (1)
- Mehretagen-AVK (1)
- Melanom (1)
- Melanoma (1)
- Mensch (1)
- Merkel cell carcinoma (1)
- Merkelzellkarzinom (1)
- Merle d´Aubigné Index (1)
- Mesenchymal progenitor cells (1)
- Mesenchymale Stammzellen (1)
- Mesenchymzelle (1)
- Mesh (1)
- Mesh Augmentation (1)
- Messerstichverletzung (1)
- Messungen (1)
- Metabolom (1)
- Metalloproteinasen (1)
- Metastase (1)
- Metastatic breast cancer (1)
- Metformin (1)
- Mice (1)
- Microsuergery (1)
- Mikroperifusion (1)
- Milz (1)
- Minimal-invasive Chirurgie (1)
- Minimalinvasiv (1)
- Minimalinvasive Leistenhernienversorgung (1)
- Minimally invasive surgery (1)
- Minipig (1)
- Minischwein (1)
- Mitochondrium (1)
- Moncyte (1)
- Monoclonal antibody (1)
- Monocytes (1)
- Monozyten-Makrophagen-System (1)
- Morbidität (1)
- Morphologie (1)
- Morphologie- und funktionsgerechte Schilddrüsenchirurgie (1)
- Morphology (1)
- Multicenter randomized-trial (1)
- Multikomponentenspende (1)
- Multilevel-PAD (1)
- Multimodal therapy (1)
- Multimodale Therapie (1)
- Multimorbidität (1)
- Multiport (1)
- Multizentrisches Register (1)
- NASH (1)
- NEKAR-Studie (1)
- NFATc1 (1)
- NK-Zellen (1)
- NK-cells (1)
- NPWT (1)
- NXA (1)
- Nabelbruch (1)
- Nabelhernien-Operation (1)
- Nabelschnur (1)
- Nabelschnurbruch (1)
- Nachsorge (1)
- Nacktmaus (1)
- Nadeln (1)
- Nahrungsergänzungsmittel (1)
- Nahtmaterialien (1)
- Narben (1)
- Narbenhernienchirurgie (1)
- Narbenhernieninzidenz (1)
- Narbenhernienklassifikation (1)
- Narbenqualitätsmessung (1)
- Nebenniere (1)
- Nebennierenkarzinom (1)
- Nebenschilddrüsenkarzinom (1)
- Netzversorgung (1)
- Neurotrophe Faktoren (1)
- Niere (1)
- No-Reflow (1)
- Nährstoffmangel (1)
- OPTOCAT-3D (1)
- OSG Fraktur (1)
- Oberflächenmodifikation (1)
- Obesity (1)
- Obturator bypass (1)
- Octreotid (1)
- Offene Abdomen Therapie (1)
- Offenheitsrate (1)
- Omphalozele (1)
- Oncology (1)
- Open abdomen therapy (1)
- Orchidopexie (1)
- Organoids (1)
- Osteochondrom (1)
- Osteoporose (1)
- Outcome (1)
- Ovarialteratom (1)
- Ovarialzyste (1)
- Oxidative Stress (1)
- P-glycoprotein (1)
- PAD (1)
- PAOD (1)
- PAVK (1)
- PBMC (1)
- PBSC (1)
- PBSCT (1)
- PCI (1)
- PCNA (1)
- PCR (1)
- PCR-ELISA (1)
- PD-1 (1)
- PD-1-Antigen (1)
- PDE (1)
- PDE4-I (1)
- PDGF (1)
- PDK (1)
- PDL-1 (1)
- PDL-2 (1)
- PET (1)
- PET imaging (1)
- PI-3-kinase (1)
- PI3K/Akt/mTOR (1)
- PLAG1 rearrangement (1)
- POEM (1)
- POEM vs. LHM bei Achalasie (1)
- PTFE (1)
- PYY3-36 (1)
- PaDaWaN (1)
- Pankreasfistel (1)
- Parathyreoidektomie (1)
- Parese (1)
- Pars flaccida (1)
- Pathway (1)
- Patienten (1)
- Patientenselektion (1)
- Patientenzufriedenheit (1)
- Pediatric malignancy (1)
- Pentaglobin (1)
- Peptidanaloge (1)
- Perfusion (1)
- Perfusionskultur (1)
- Pergastric technique (1)
- Pergastrische Technik (1)
- Periduralkatheter (1)
- Perineuralscheidenbefall (1)
- Periphere Stammzellentransplantation (1)
- Peritonectomy (1)
- Peritonektomie (1)
- Perkutane transluminale Angioplastie (1)
- Perorale endoskopische Myotomie (POEM) (1)
- Peyton-Methode (1)
- Pfortader (1)
- Pharmakologie (1)
- Phimosis (1)
- Physikalische (1)
- Physiological studies (1)
- Phänotyp (1)
- Pilonfraktur (1)
- Pionfracture (1)
- Plateau-Quotient (1)
- Plattenosteosynthese (1)
- Platzwunden (1)
- Polyesterprothese (1)
- Polymerase-Kettenreaktion (1)
- Polymermembran (1)
- Polytrauma (1)
- Porcine (1)
- Posteriore Komponentenseparation (1)
- Postischämischer Reperfusionsschaden (1)
- Postoperative Analgesie (1)
- Postoperative complications (1)
- Pouchdilatation (1)
- Predict fluid responsiveness (1)
- Predictors (1)
- Prediktoren (1)
- Prevot-Nagelung (1)
- Primary hyperparathyroidism (pHPT) (1)
- Primärer Hyperparathyreoidismus (1)
- Probanden (1)
- Prognosefaktor (1)
- Prognosemarker (1)
- Prognosis (1)
- Prognostic marker (1)
- Proliferation (1)
- Proliferationsmarker (1)
- Prostaglandine (1)
- Prostaglandins (1)
- Protein p53 (1)
- Proteintyrosinphosphatase (1)
- Protonenpumpenblocker (1)
- Prävention (1)
- Pulmonary function tests (1)
- Puls-pressure variation (1)
- Pyloromyotomie (1)
- Pylorusstenose (1)
- Quality assurance (1)
- Quality of packed red cells (1)
- Qualität (1)
- Qualitätssicherung (1)
- RET6 (1)
- RNAPOL1 (1)
- RNS Polymerase I (1)
- Radical prostatectomy (1)
- Radikalität (1)
- Radiochemotherapy (1)
- Randomisierung (1)
- Randomized controlled-trial (1)
- Raucherbein (1)
- Real time quantitative PCR (1)
- Recurrence (1)
- Red Blood Cells (1)
- Redifferenzierung (1)
- Refluxrezidive (1)
- Regenerative Medizin (1)
- Registerdaten (1)
- Registerstudie (1)
- Regulationsmechanismen immunologischer Toleranz (1)
- Regulator T-Lymphozyten (1)
- Regulatorische T-Lymphozyten (1)
- Regulatorische T-Zellen (1)
- Regulatorischer T-Lymphozyt (1)
- Reifung (1)
- Rekonvaleszenz (1)
- Rektumchirurgie (1)
- Rektumkarzinom (1)
- Rektumtumor (1)
- Resection (1)
- Resektionsabstand (1)
- Retromuskuläres Netz (1)
- Retrorektus Netz (1)
- Retrospektive Studie (1)
- Reverse Transkriptase-Polymerase-Kettenreaktion (1)
- Rheologie (1)
- Rho-Kinasen (1)
- RhoA (1)
- Ribosom (1)
- Risikofaktoren (1)
- Risk (1)
- Roboter (1)
- Roux-en-Y gastric bypass (1)
- Ruptur (1)
- Rupturiertes abdominelles Aortenaneurysma (1)
- SAPS 3 (1)
- SAterilschlauchschweißgerät (1)
- SOFA (1)
- SSI (1)
- ST18 (1)
- STT Fusion (1)
- Schaf (1)
- Schilddrüsenkarzinom (1)
- Schmerz (1)
- Schockraum (1)
- Schussverletzung (1)
- Score (1)
- Secondary traumatic brain damage (1)
- Seit-zu-End-Anastomose (1)
- Sekundärkrankheit (1)
- Sekundärnaht (1)
- Selection of patients (1)
- Serom (1)
- Severe Acute Respiratory Syndrome Coronavirus 2 (1)
- Sicherheit (1)
- Sigmadivertikulitis (1)
- Signaltransduktion (1)
- Silber (1)
- Silikon (1)
- Simulationmodel (1)
- Simulationsmodell (1)
- Single Port Cholezystektomie (1)
- Single-Port (1)
- Sirolimus (1)
- Sleeve-Resektion (1)
- Slippage (1)
- Soft tissue sarcoma (1)
- Speiseröhrenkrebs (1)
- Spinalanästhesie (1)
- Spondylodese (1)
- Spontantoleranz (1)
- Spätresultate (1)
- Stabilität (1)
- Staging (1)
- Stammzellenfaktor (1)
- Staphylococcus aureus (1)
- Stapler (1)
- Stationäre Behandlung (1)
- Stellenwert (1)
- Stent (1)
- Sterblichkeit (1)
- Sterilisierung (1)
- Steroidapplikation (1)
- Steroidtherapie (1)
- Stickstoffmonoxid (1)
- Stimulation (1)
- Stoma (1)
- Stomarückverlagerung (1)
- Strahlendurchlässigkeit (1)
- Stromal tumor (1)
- Stromatumor (1)
- Supportivprodukt (1)
- Supragenualer femoropoplitealer Bypass (1)
- Suprakondyläre Humerus Fraktur (1)
- Surface modification (1)
- Surgical and invasive medical procedures (1)
- Surgical education (1)
- Surgical oncology (1)
- T cell clone (1)
- T cell mediated immune response (1)
- T cell receptors (1)
- T cells (1)
- T lymphocytes (1)
- T-Lymphozyten (1)
- T-Zell Klon (1)
- T-Zell-Aktivierung (1)
- T-Zell-Immunantwort (1)
- T-Zellrezeptor-Aufbau (1)
- T-cell activation (1)
- T-cell inhibition (1)
- T-lymphocyte (1)
- TCR (1)
- TCR signaling cascade (1)
- TEER (1)
- TGF ß (1)
- TLR2 (1)
- TLR4 (1)
- TLR9 (1)
- TME (1)
- TNF (1)
- TNF receptor superfamily (1)
- TREC (1)
- Target (1)
- Taurolin (1)
- Telomere (1)
- Teratom (1)
- Terroranschlagtrauma (1)
- Th1 (1)
- Th2 (1)
- Therapie HCC (1)
- Therapieerfolg (1)
- Therapieergebnisse (1)
- Thermotolerance (1)
- Thermotoleranz (1)
- Thoraxmagen (1)
- Thrombosis (1)
- Thyreoidektomie (1)
- Thyroid gland (1)
- Thyroid surgery (1)
- Tigecyclin (1)
- Time interval (1)
- Tissue (1)
- Tolerance (1)
- Tolerance-Induction (1)
- Toleranz-Induktion (1)
- Toll-like Rezeptoren (1)
- Toll-like receptor signaling (1)
- Toll-like receptors (1)
- Topometrie (1)
- Toupet (1)
- Trampolin (1)
- Trampoline injuries (1)
- Trampolinverletzungen (1)
- Transplantat (1)
- Transplantate (1)
- Trendelenburg´s sign (1)
- Tryptophanstoffwechsel (1)
- Tumor Microenvironment (1)
- Tumor suppressor genes (1)
- Tumorantigen CA 19-9 (1)
- Tumorbiopsie (1)
- Tumorerkrankungen (1)
- Tumorimmunantwort (1)
- Tumorstammzellmarker (1)
- Tumorstoffwechsel (1)
- Tumorsuppressorgene (1)
- Tumorwachstum (1)
- Tumorzelladhäsion (1)
- Umbilical Hernia Operation (1)
- Umbilical-hernia (1)
- Unterarmbruch (1)
- Unterarmfraktur (1)
- Unterdrucktherapie (1)
- Unterversorgung (1)
- Up-regulation (1)
- Urachus (1)
- V. saphena magna (1)
- V.A.C.-Therapie (1)
- VAC (1)
- VE-PTP (1)
- VLBW (1)
- Va (1)
- Vakuumtherapie (1)
- Validation (1)
- Validierung (1)
- Valtrac (1)
- Vene (1)
- Ventrale Hernie (1)
- Venöse (1)
- Verbrennung (1)
- Verbrennung <Medizin> (1)
- Verbrennungen und Verbrühungen (1)
- Verbrühung (1)
- Vergleich (1)
- Verkapselung (1)
- Verlauf (1)
- Versorgungsstudie (1)
- Vitamin C (1)
- Vogt (1)
- Vorsorgekoloskopie (1)
- Warburg Effekt (1)
- Warburg effect (1)
- Weichteilsarkom (1)
- Weichteilsarkome der Extremität (1)
- Weichteilsarkome des Retroperitoneums (1)
- Weizen (1)
- Weizenkeimextrakt (1)
- Wharton´s jelly (1)
- Wistarratte (1)
- Wound-healing (1)
- Wuerzburg Woundscore (1)
- Wundbehandlung (1)
- Wundfarbe (1)
- Wundheilungsstörung (1)
- Wundinfektion (1)
- Wurmfortsatzentzündung (1)
- Würzburger Wundscore (1)
- Xenogen Transplantation (1)
- Xenogene Transplantation (1)
- Y-gastric bypass (1)
- Zellulartherapie (1)
- ZfKD (1)
- Zucker (1)
- Zucker (fa/fa) Ratten (1)
- Zucker (fa/fa) rats (1)
- Zucker fatty fa/fa rats (1)
- Zwerchfellkrankheit (1)
- Zytokeratin (1)
- Zytokinetisch (1)
- Zytoreduktion (1)
- [11C]-Choline PET/CT (1)
- [11C]-Methionine (1)
- [18F]FDG-PET-CT (1)
- [99mTc]-Sestamibi scan (1)
- abdominal compartment syndrome (1)
- abdominal lymph node metastases (1)
- abdominal surgery (1)
- abdominal wall hernia (1)
- abdominal wall surgery (1)
- above-knee femoropopliteal bypass (1)
- accumulation (1)
- achalasia (1)
- acute (1)
- acute and chronic mesenteric ischemia (1)
- acute appendicitis (1)
- acute kidney injury (1)
- acute liver failure (1)
- acute respiratory distress syndrome (1)
- adipose-derived stromal/stem cells (ASCs) (1)
- adiposity (1)
- adjuvant (1)
- adrenal cancer (1)
- adrenal surgery (1)
- adrenal tumors (1)
- adrenalectomia (1)
- adrenocortical (1)
- adrenocortical adenocarcinoma (1)
- advancement flap (1)
- afp (1)
- akute und chronische mesenteriale Ischämie (1)
- alignment (1)
- alloantigen (1)
- allogene MHC-Peptide (1)
- allogene Nierentransplantation (1)
- allogene Transplantation (1)
- allogeneic transplantation (1)
- allograft (1)
- allograft rejection (1)
- allopeptide (1)
- allorecognition (1)
- allotransplantation (1)
- alpha-Liponsäure (1)
- alternative splicing (1)
- alveolar epithelium (1)
- alveolar macrophages . rat . lung (1)
- anal abszess (1)
- anal atresia (1)
- anal fistula (1)
- anal incontinence (1)
- anale Inkontinenz (1)
- anaplastic large cell lymphoma (1)
- anastomotic leak (1)
- anastomotic recurrence (1)
- aneurysm (1)
- anorectal abscess (1)
- anorectal angl (1)
- anorectal malformation (1)
- anteriore 180°-Hemifundoplikatio (1)
- anti-VEGF Antikörper (1)
- anti-VEGF-antibody (1)
- anti-tumor effects (1)
- antibiotic prescribing quality (1)
- antibiotic prescription behavior (1)
- antibiotic resistance (1)
- antigen (1)
- antigen-specific therapy (1)
- antigenspezifische Therapie (1)
- antioxidant (1)
- antireflux surgery (1)
- antithrombotic therapy (1)
- aortobifemoral bypass (1)
- aortobifemoraler Bypass (1)
- aortodistal graft (1)
- aortodistale Prothese (1)
- appendectomy (1)
- appendix (1)
- arterial grafts (1)
- artery (1)
- arthroscopy (1)
- arthroscopy of the wrist (1)
- ascorbic acid (1)
- assisted reproductive techniques (1)
- autostimulation (1)
- axilla (1)
- axilläre (1)
- bacterial overgrowth (1)
- bakterielle Besiedelung (1)
- bariatric (1)
- bariatrische (1)
- bariatrische Operation (1)
- barium swallow (1)
- barrett-esophagus (1)
- barrier models (1)
- benign (1)
- benigne (1)
- benzoquinone (1)
- biliopancreatic diversion (1)
- biocompatible patch (1)
- biofeedback therapy (1)
- biokompatibel (1)
- biokompatibler Patch (1)
- biological models (1)
- biologische Aktivität (1)
- biopsy (1)
- bioreactor (1)
- birth defects (1)
- bladder (1)
- blood samples (1)
- blood-nerve barrier (1)
- blood–brain barrier (1)
- body fat (1)
- body mass index (BMI; kg/m\(^2\)) (1)
- body weight (1)
- bone cyst (1)
- bone marrow (1)
- bone mineral density measurement (1)
- bone tumor (1)
- brain dead (1)
- brain metastases (1)
- brain plasticity (1)
- breast cancer (1)
- breast implants (1)
- bronchopulmonary dysplasia (1)
- brown adipose tissue (1)
- bsiphosphonate treatment (1)
- bullae (1)
- burn (1)
- burns (1)
- burns and scalds (1)
- burst abdomen (1)
- bypass (1)
- bypass surgery (1)
- cAMP (1)
- ca-antagonists (1)
- caco-2 cells (1)
- cadaver multiorgan preservation (1)
- caloric-restriction (1)
- cancer cells (1)
- cancer diagnosis (1)
- cancer in childhood (1)
- cancer risk (1)
- cancer treatment (1)
- cancers and neoplasms (1)
- capillary leak (1)
- capture (1)
- carcinogenesis (1)
- carcinoma metastases to pancreas (1)
- carcinomas (1)
- cardiogenic (1)
- catgut (1)
- catheterization (1)
- catheters (1)
- cea (1)
- cell cycle and cell division (1)
- cell replacement therapy (1)
- cellular senescence (1)
- cementless total hip arthroplasties (1)
- cerebEND (1)
- cerebral metastases (1)
- certainty of diagnosis (1)
- chemistry (1)
- chemokine receptor (1)
- chemoradiation (1)
- chemotherapeutics (1)
- child (1)
- children born (1)
- chimerism (1)
- chirurgische Expertise (1)
- chirurgisches Nahtmaterial (1)
- cholangiozellulär (1)
- cholestasis (1)
- chondrocytes (1)
- chronic wounds (1)
- chronisch venöse Insuffizienz (1)
- chronisch-entzündliche Darmerkrankung (1)
- chronische Abstossung (1)
- chronische Abstoßung (1)
- chronische Schmerzen (1)
- chronische Wunde (1)
- chylomicron (1)
- classification (1)
- clinical outcome (1)
- colloids (1)
- colon carcinoma cells (1)
- colon resection (1)
- colorectal abdominal closure (1)
- colorectal surgery (1)
- colorektal cancer (1)
- colorimetry (1)
- combined treatment (1)
- comet assay (1)
- comorbidities (1)
- comparison (1)
- computed tomography angiography (CTA) (1)
- congenital malformations (1)
- container (1)
- continuous extracorporeal femoral perfusion model (1)
- convalescence (1)
- conventional laparoscopic appendectomy (1)
- cord blood (1)
- correlation between cd34+ in peripheral blood (1)
- cost effectiveness (1)
- cost-effectiveness (1)
- costimulatorische Signalwege (1)
- costimulatory pathways (1)
- costs (1)
- cotransporter SGLT1 (1)
- course (1)
- creatinine (1)
- curative resection (1)
- cyclophosphamide (1)
- cyclosporine (1)
- cytapheresis (1)
- cytostatic (1)
- cytotoxicity (1)
- damage control surgery (1)
- dass II antigen blockade (1)
- datawarehousing (1)
- defaecography (1)
- dendritische Zellen (1)
- descriptive epidemiology (1)
- desmoglein (1)
- dexamethasone (1)
- diabetes mellitus (1)
- diagnostics (1)
- difference (1)
- differentiation (1)
- digital subtraction angiography (DSA) (1)
- discharge definition (1)
- distal radiusfracture (1)
- distale Radiusfraktur (1)
- distale Unterschenkelfraktur (1)
- doppel-blind (1)
- drug-eluting beads (1)
- drugs (1)
- dual-room whole-body CT (1)
- duodenal atresia (1)
- duodenal jejunal bypass (1)
- duodenal obstruction (1)
- duodenal perforation (1)
- duodenal trauma (1)
- duodenale Obstruktion (1)
- edovascular (1)
- efficiency (1)
- elbow (1)
- elective surgery (1)
- ellbow (1)
- emergency room (1)
- encapsulated islets alginate (1)
- end-to-end-anastomosis (1)
- endocrine (1)
- endokrin (1)
- endoscopic (1)
- endoscopic full thickness resection (eFTR) (1)
- endoscopic groin hernia repair (1)
- endosonography (1)
- endosponge (1)
- endotoxemia (1)
- endotoxinemia (1)
- endovascular repair (1)
- endovaskulär (1)
- energy homeostasis (1)
- enteric glial cells (1)
- enteric nervous system (1)
- enteroid (1)
- enteroids (1)
- enteroinsulinäre Achse (1)
- ephitelial cells (1)
- epidural block (1)
- epigastric hernia (1)
- epithelial (1)
- epithelial cells (1)
- epithelium (1)
- erratum (1)
- erythrocyte (1)
- esophageal perforation (1)
- esophagus (1)
- expansion (1)
- expenditure (1)
- experimental models of disease (1)
- experimental therapy (1)
- extreme Adipositas (1)
- extremity trauma (1)
- fascial closure (1)
- fast track (1)
- fat absorption (1)
- fat appetite (1)
- fatty liver (1)
- fecal (1)
- fecal incontinence (1)
- femoral arteries (1)
- femoral hernia (1)
- femoro-crural (1)
- fermented wheat germ (1)
- fibrin gel in vitro (1)
- fish bone (1)
- fistulizing Crohn’s Disease (1)
- focused surgical approach (1)
- folinic acid (1)
- follow-up examination (1)
- food intake (1)
- foreign body ingestion (1)
- foxp3 (1)
- fracture (1)
- fracture-associated vascular damage (1)
- full-thickness resection device (FTRD) (1)
- fundoplikation (1)
- fäkal (1)
- gastric banding operation (1)
- gastric-bypass (1)
- gastric-bypass surgery (1)
- gastroesophageal reflux disease (1)
- gastrointestinal cancer (1)
- gastrointestinal infections (1)
- gastrointestinal perforation (1)
- gastrointestinal tract (1)
- gastroschisis (1)
- gemcitabine (1)
- gemischte Leukozytenkultur (1)
- gene expression (1)
- gene regulation (1)
- generation (1)
- geriatric (1)
- germ cell tumor (1)
- germline mutation (1)
- gesundheitsbezogene Lebensqualität (1)
- glucagon like peptide-1 (1)
- glucocorticoid receptor (1)
- glucose homeostasis (1)
- glucose metabolism (1)
- glucose starvation (1)
- glutamine (1)
- graft (1)
- groin (1)
- groin hernia (1)
- growth differentiation factor 15 (1)
- gut hormones (1)
- health-related quality of life (1)
- hematology (1)
- hemorrhagic (1)
- henoch-schönlein purpura (1)
- hepatic surgery (1)
- hernia defect (1)
- hernia repair material (1)
- heterologous (1)
- histocompatibility (1)
- histologic diversity (1)
- histopathology (1)
- hormonsubstitution (1)
- hrQOL (1)
- human (1)
- human biomonitoring (1)
- human cancer (1)
- human melanoma (1)
- human protein solution (1)
- hypersensitivity (1)
- hypertension (1)
- hypertrophe Narben (1)
- hypertrophic scar (1)
- hypertrophic scars (1)
- hypocalcemia (1)
- hypothalamic gene expression (1)
- hypoxia (1)
- idiopathic fecal incontinence (1)
- ileal conduit (1)
- ileostomy reversal (1)
- imaging (1)
- immaturity (1)
- immundominant (1)
- immune check inhibitor (1)
- immune response (1)
- immunity (1)
- immunodominant (1)
- immunoglobulin (1)
- immunohistology (1)
- immunological tolerance (1)
- immunoreaction (1)
- immunotherapeutics (1)
- impaired woundhealing (1)
- impedance spectroscopy (1)
- imperforate anus (1)
- in vitro analyses (1)
- in vitro model (1)
- in vivo (1)
- in-vitro (1)
- in-vitro fertilization (1)
- incidence (1)
- incisional abdominal wall hernia (1)
- incompetence of lower esophageal sphincter (1)
- indirect pathway of allorecognition (1)
- indirekte Alloantigenerkennung (1)
- induced obesity (1)
- infant (1)
- infants born (1)
- infectivity (1)
- infektion (1)
- inflammation-induced tissue demage (1)
- inflammatory cell model (1)
- infragenual (1)
- inguinal lymph node dissection (1)
- inguinale Lymphadenektomie (1)
- injury (1)
- insulin resistance (1)
- insulinsensitivity (1)
- intercellular junctions (1)
- intestinal barrier (1)
- intestinal glucose (1)
- intestinal metaplasia (1)
- intestinal microbiota (1)
- intestinal organoids (1)
- intestinale Epithelbarriere (1)
- intestine (1)
- intima hyperplasia (1)
- intraabdominal abscess (1)
- intragastric balloon (1)
- intrahepatic (1)
- intrahepatisch (1)
- intramedullary nailing (1)
- intramedulläre Nagelung (1)
- intravascular ultrasound (IVUS) (1)
- intravenous (1)
- intravenous glucose tolerance test (1)
- inzisionale Hernie (1)
- ionizing radiation (1)
- irinotecan (1)
- islet transplantation (1)
- isocaloric intake (1)
- isosteviol sodium (STVNA) (1)
- kappa-B (1)
- keloids (1)
- ketogenic dients (1)
- ketogenic diet (1)
- keton bodies (1)
- ketone bodies (1)
- kidney (1)
- kidney allograft (1)
- kidneys (1)
- klinische Ergebnisse (1)
- knee-endoprothesis (1)
- kolorektal (1)
- kolorektale Chirurgie (1)
- kongenital (1)
- kongenitale Zwerchfellhernie (1)
- konservative Therapie (1)
- künstlicher Darmausgang (1)
- la antigens (1)
- lactate (1)
- lapararoskopische IPOM (1)
- laparoscopic appendectomy (1)
- laparoscopic cholecystectomy (1)
- laparoscopic fundoplication (1)
- laparoscopic right colectomy (1)
- laparoscopy (1)
- late onset sepsis (1)
- latency time (1)
- learning curve (1)
- left hemicolectomy (1)
- leitlinienorientierte Therapie (1)
- leptin (1)
- leptin system (1)
- leukemia (1)
- level of evidence: IV (1)
- ligament-tension (1)
- lipoblastoma (1)
- lipodystrophy (1)
- liver function capacity (1)
- liver metastases (1)
- liver resection (1)
- liver transplantation (1)
- liver/small bowel transplantation (1)
- local steroid application (1)
- localization (1)
- locally advanced disease (1)
- long term follow-up (1)
- longbone-fracture (1)
- low molecular heparin (1)
- low-grade mucinous neoplasm (1)
- low-molecular heparin (1)
- low-risk intra-abdominal infections (1)
- lumbar spine (1)
- lung resection (1)
- lymph node dissection (1)
- lymph nodes (1)
- lymphadenctomy (1)
- lymphadenectomy (1)
- lymphatic fistula (1)
- lymphocele (1)
- lymphocytes (1)
- lymphomatoid papulosis (1)
- macrophage (1)
- magnetic resonance imaging (1)
- maintain immune homeostasis (1)
- malignes Melanom (1)
- malnutrition (1)
- mammographie (1)
- management (1)
- manometry (1)
- manual suture (1)
- margin (1)
- mass (1)
- mastocytosis (1)
- measles virus (1)
- measurable criterion (1)
- mechanical bowel preparation (1)
- medial patellofemoral ligament (1)
- meningitis (1)
- mesenchymal stem cells (1)
- mesh augmentation (1)
- mesh repair (1)
- meshes (1)
- metaanalysis (1)
- metabolic profile (1)
- metabolically unhealthy obesity (1)
- metabolisch-bariatrische Operation (1)
- metachronous (1)
- meßbare Kriterien (1)
- mhc (1)
- miRNA expression (1)
- mice (1)
- microbiota (1)
- microencapsulated (1)
- microencapsulation (1)
- mikroverkapselt (1)
- mikroverkapselte Inseln in vitro (1)
- mild hypothermia (1)
- minimal invasive Parathyreiodektomie (1)
- minimal invasive parathyroidectomy (1)
- minimalinvasiv (1)
- minimally invasive (1)
- mixed leukocyte culture (1)
- mixed leukocyte reaction (1)
- mold exposure (1)
- molecular diagnostics (1)
- monocyte subsets (1)
- morphology- and the function-appropriate thyroid surgery (1)
- mortality rate (1)
- mouse (1)
- mucormycosis (1)
- mucosal healing (MH) (1)
- multimodal (1)
- multizentrisch (1)
- myeloid (1)
- nab-paclitaxel (1)
- needle (1)
- negative pressure wound therapy (1)
- neoadjuvant (1)
- neoadjuvant chemotherapy (1)
- neonatal immunology (1)
- neonatal meningitis (1)
- neonatal outcome (1)
- netrin-1 (1)
- neue Klassifikation (1)
- neuroblastoma – diagnosis (1)
- neuroendocrine tumor (NET) (1)
- neuroinflammation (1)
- neuropathic pain (1)
- neurotrophic factors (1)
- nitric oxide (1)
- no-reflow (1)
- noradrenaline (1)
- noradrenaline transporter (1)
- nosocomial pneumonia (1)
- nosokomial (1)
- nosokomiale Pneumonie (1)
- novel human cadaveric perfusion model (1)
- obesity surgery (1)
- occlusion (1)
- octogenarians (1)
- octogenerians (1)
- octreotide (1)
- offenes IPOM (1)
- older adult (1)
- omphalocele (1)
- oncogene-induced senescence (1)
- oncology (1)
- open appendectomy (1)
- open wound treatment (1)
- operative (1)
- operative Therapie (1)
- operative therapy (1)
- oral anticoagulants (1)
- organoid (1)
- osmotic resistence (1)
- osmotische Resistenz (1)
- osteochondroma (1)
- osteoporosis (1)
- osteosynthesis with plates (1)
- outcome forschung (1)
- outcome investigation (1)
- outcome of therapy (1)
- ovarian cyst (1)
- pH (1)
- pain (1)
- pancreas Islets (1)
- pancreatectomy (1)
- pancreatic adenocarcinoma (PDAC) (1)
- pancreatic fistula (1)
- pancreatic head cancer (1)
- pancreaticoduodenectomy (1)
- parathyroid adenoma (1)
- parathyroid carcinoma (1)
- parathyroid cells (1)
- parathyroid glands (1)
- parathyrroid gland (1)
- paresis (1)
- patella alta (1)
- patella dislocation (1)
- patella instability (1)
- patency-rate (1)
- pathway analysis (1)
- patient satisfaction (1)
- patient-derived organoid (PDOs) (1)
- patient-derived tumor organoid (PDTO) (1)
- patients (1)
- pediatric adrenocortical adenoma (1)
- pediatric adrenocortical cancer (1)
- pediatric adrenocortical tumor (1)
- pediatric surgery (1)
- pelvic floor disorders (1)
- pelvic trauma (1)
- pembrolizumab (1)
- pemphigus (1)
- penetrating ileitis (1)
- pentaglobin (1)
- peptide analogs (1)
- peptide tyrosine tyrosine 3-36 (PYY3-36) (1)
- peptide tyrosine tyrosine 3-36 (PYY\(_{3-36}\)) (1)
- perfusion-culture (1)
- perianal fistulas (1)
- perifusion (1)
- perineural invasion (1)
- perioperative Kinetik (1)
- perioperative alterations (1)
- perioperative antibiotic prophylaxis (1)
- perioperative chemotherapy (1)
- peripartal management (1)
- peripartales Management (1)
- peripheral arterial occlusive disease (1)
- peripheral nerve evaluation (1)
- periphere Nervenevaluation (1)
- periphery-brain interactions (1)
- permeability (1)
- peroral endoscopic myotomy (1)
- phimosis (1)
- phosphodiesterase-4 (1)
- phosphodiesterase-inhibitors (1)
- photon-counting computed tomography (CT) (1)
- pig or swine (1)
- placebo-kontrolliert (1)
- plateau-ratio (1)
- platelet dysfunction (1)
- polyester graft (1)
- popliteal aneurysm (1)
- porcine islets (1)
- porzine Langerhans-Inseln (1)
- positron emission tomography imaging (1)
- post-mortem heart recovery (1)
- post-operative antibiotic treatment (1)
- posterior component separation (1)
- posteriore Komponentenseparation (1)
- postoperative Komplikationen (1)
- postoperative Morbidität (1)
- postoperative Schmerzen (1)
- postoperative analgesia (1)
- postoperative inflammation (1)
- postoperative morbidity (1)
- preclinical research (1)
- predictors of failure (1)
- preoperative localization (1)
- pressure measurement (1)
- pressure otimum (1)
- pressure sore (1)
- pressure values (1)
- preterm birth (1)
- prevalence (1)
- primärer Hyperparathyreoidismus (1)
- probands (1)
- prognostic factor (1)
- prognostische Faktoren (1)
- prospective studies (1)
- prosthesis (1)
- protein expression (1)
- protein kinase D2/PKD2/PRKD2 (1)
- protein synthesis (1)
- proton pump inhibitors (1)
- pyloric stenosis (1)
- pyloromyotomy (1)
- quality (1)
- questionnaire (1)
- r-TAPP (1)
- radicality (1)
- radiolucency (1)
- radiotracer (1)
- randomised double-blind study (1)
- rapamycin (1)
- reactivating p53 and inducing tumor apoptosis (RITA) (1)
- reactive oxygen (1)
- reactive oxygen species (1)
- real-time glucose monitoring (1)
- rectal carcinoma (1)
- rectal surgery (1)
- rectal tumour (1)
- recurrence rate (1)
- recurrence survival (1)
- recurrent abdominal wall hernia (1)
- recurrent nerve (1)
- recurrent nerve paralysis (1)
- redifferentiation (1)
- reflux recurrence (1)
- regeneration (1)
- regenerative Medicine (1)
- regenerative medicine (1)
- regional recurrence (1)
- regionalization and organoids (1)
- registries (1)
- regulatory T-cells (1)
- rejection (1)
- reliability (1)
- renal cell carcinoma (1)
- renal transplantation (1)
- repeat surgery (1)
- repeated surgery (1)
- reproductive medicine (1)
- rerouting procedure (1)
- resection (1)
- resuscitation time (1)
- retromuscular mesh (1)
- retromuskuläres Netz (1)
- retroperitoneal sarcoma (1)
- retroperitoneal tumor (1)
- retrorectus mesh (1)
- retrorektus Netz (1)
- retrospective (1)
- retrospective analysis (1)
- retrospektiv (1)
- retrospektive Analyse (1)
- reverse transcriptase-polymerase chain reaction (1)
- review (1)
- rheology (1)
- ribosomale RNS (1)
- ribosome (1)
- risk factor (1)
- riskfaktors (1)
- robot-assisted hip replacement surgery (1)
- roboterassistierte Hüftprothetik (1)
- robotic surgery (1)
- robotic surgical procedures (1)
- robotische Leistenhernienversorgung (1)
- rolipram (1)
- roux y (1)
- roux y Magenbypass (1)
- roux-en-y gastric bypass (1)
- rupture (1)
- ruptured abdominal aortic aneurysm (1)
- rygb (1)
- sVE-Cadherin (1)
- sacral neuromodulation (1)
- sakrale Nervenstimulation (1)
- sarcoma of the extremity (1)
- scald (1)
- scars (1)
- schock (1)
- schwere Sepsis (1)
- screening (1)
- screening colonoscopy (1)
- seahorse (1)
- secondary skin closure (1)
- secretion (1)
- selective and systematic lymphadenectomy (1)
- selektive und systematische Lymphadenektomie (1)
- semifundoplikation (1)
- seminoma (1)
- semizirkulärer Umbilikalschnitt (1)
- septic (1)
- seroma (1)
- serotonin (1)
- serotonin transporter (1)
- severe Sepsis (1)
- severity score (1)
- sheep (1)
- shooting injuries (1)
- sichtbare Netze (1)
- side-to-end-anastomosis (1)
- silicone (1)
- silver (1)
- single-port appendectomy (1)
- sirs (1)
- sleeve gastrectomy (1)
- small bowel (1)
- small interferring RNA (1)
- small pixel effect (1)
- spondylodesis (1)
- spontaneous liver allograft tolerance (1)
- stab wounds (1)
- stem cell based therapies (1)
- stem cell yield (1)
- sterile docking (1)
- sterilization (1)
- stimulation (1)
- streptozotocin (1)
- super-obesity (1)
- superior (1)
- supportive agent (1)
- supracondylar humerus fracture (1)
- suprapubisch (1)
- surgical (1)
- surgical and invasive medical procedures (1)
- surgical care (1)
- surgical complications (1)
- surgical intra-abdominal infections (1)
- surgical management (1)
- surgical oncology (1)
- surgical outcome (1)
- surgical procedures (1)
- surgical proficiency level (1)
- surgical resection (1)
- surgical site infection (1)
- surgical site infections (1)
- surgical sutures (1)
- surgical trauma room (1)
- surgical treatment (1)
- surgical zipper (1)
- survival analysis (1)
- survival rates (1)
- suture material (1)
- sympathetic nervous system (1)
- systematic review (1)
- systemic and cutaneous CD30+ lymphoproliferations (1)
- systemic endotoxemia (1)
- technology (1)
- terminal ileitis (1)
- terror attack trauma (1)
- thermogenesis (1)
- thrombogenicity (1)
- thyroid (1)
- thyroid cancer (1)
- thyroid gland surgery (1)
- thyroiditis (1)
- tight junction (1)
- tight junction proteins (1)
- tissue (1)
- total pancreatectomy (1)
- transanal endoscopic microsurgery (TEM) (1)
- transarterial chemoembolization (1)
- translation (1)
- translation initiation (1)
- transpedicular (1)
- transpedikulär (1)
- transplant (1)
- transplantation immunology (1)
- transstomal endoluminal vacuum therapy (1)
- transverse fascia (1)
- trauma care (1)
- trauma centre (1)
- trauma management (1)
- trochlear dysplasia (1)
- trophic factors (1)
- tumor cell adhesion (1)
- tumor disease (1)
- tumor growth (1)
- tumor-associated antigen (1)
- tumorassoziiertes Antigen (1)
- type 2 diabetes (1)
- ultrahigh resolution (1)
- umbilical cord (1)
- umbilical incision (1)
- uncoupling protein 1 (1)
- undertreatment of osteoporosis (1)
- urinary tract infections (1)
- urine (1)
- urothelial tissue engineering (1)
- vascualr surgery (1)
- vascular prosthesis (1)
- vascular type (1)
- vasculitis (1)
- ve-cadherin (1)
- venous ulcer (1)
- ventral hernia model (1)
- ventrale Hernie (1)
- visceral fat (1)
- vitality-staining (1)
- vitamin D (1)
- volume (1)
- warfarin interruption (1)
- washed erythrocytes (1)
- weight loss (1)
- weight-gain (1)
- wheat (1)
- wheat germ extract (1)
- wound healing (1)
- wound infection (1)
- wound management (1)
- wound-coulour (1)
- wrist (1)
- x (1)
- xenogene Inselzelltransplantation (1)
- xenogeneic transplantation (1)
- xenogenic transplantation (1)
- xenotransplantation (1)
- xx (1)
- »differential stress resistance« (1)
- Ösophagitis (1)
- Ösophagusatresie (1)
- Ösophaguskarzinom (1)
- Überlebensrate (1)
- Überlebensraten (1)
- Überlebenswahrscheinlichkeit (1)
- Überlebenszeitanalyse (1)
- β-Hydroxybutyrate (1)
Institute
- Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I) (503)
- Theodor-Boveri-Institut für Biowissenschaften (34)
- Medizinische Klinik und Poliklinik II (23)
- Comprehensive Cancer Center Mainfranken (20)
- Medizinische Klinik und Poliklinik I (19)
- Institut für diagnostische und interventionelle Radiologie (Institut für Röntgendiagnostik) (16)
- Klinik und Poliklinik für Anästhesiologie (ab 2004) (15)
- Kinderklinik und Poliklinik (14)
- Pathologisches Institut (13)
- Institut für Anatomie und Zellbiologie (8)
Sonstige beteiligte Institutionen
- Krankenhaushygiene und Antimicrobial Stewardship (2)
- Abteilung für Molekulare Onkoimmunologie (1)
- Harvard Medical School, Boston, USA (1)
- Institut für Medizinische Lehre und Ausbildungsforschung, Universität Würzburg (1)
- Klinikum Fulda (1)
- Klinikum Main-Spessart Lohr (1)
- Krankenhaushygiene und Antimicrobial Stewardship (Universitätsklinikum) (1)
- Krankenhaushygiene und Antimicrobial Stewardship, Universitätsklinikum Würzburg (1)
- Lehrkrankenhaus der Universität Würzburg: Klinikum Main-Spessart (1)
- Lehrstuhl für Tissue Engineering und Regenerative Medizin der Universität Würzburg (1)
The immune suppressants cyclosporin A (CsA) and tacrolimus (FK506) are used worldwide in transplantation medicine to suppress graft rejection. Both CsA and FK506 inhibit the phosphatase calcineurin (CN) whose activity controls the immune receptor-mediated activation of lymphocytes. Downstream targets of CN in lymphocytes are the nuclear factors of activated T cells (NFATs). We show here that the activity of NFATc1, the most prominent NFAT factor in activated lymphocytes supports the acute rejection of heterotopic heart allografts. While ablation of NFATc1 in T cells prevented graft rejection, ectopic expression of inducible NFATc1/αA isoform led to rejection of heart allografts in recipient mice. Acceptance of transplanted hearts in mice bearing NFATc1-deficient T cells was accompanied by a reduction in number and cytotoxicity of graft infiltrating cells. In CD8\(^+\) T cells, NFATc1 controls numerous intracellular signaling pathways that lead to the metabolic switch to aerobic glycolysis and the expression of numerous lymphokines, chemokines, and their receptors, including Cxcr3 that supports the rejection of allogeneic heart transplants. These findings favors NFATc1 as a molecular target for the development of new strategies to control the cytotoxicity of T cells upon organ transplantation.
Background
Deregulated expression of MYC is a driver of colorectal carcinogenesis, suggesting that decreasing MYC expression may have significant therapeutic value. CIP2A is an oncogenic factor that regulates MYC expression. CIP2A is overexpressed in colorectal cancer (CRC), and its expression levels are an independent marker for long-term outcome of CRC. Previous studies suggested that CIP2A controls MYC protein expression on a post-transcriptional level.
Methods
To determine the mechanism by which CIP2A regulates MYC in CRC, we dissected MYC translation and stability dependent on CIP2A in CRC cell lines.
Results
Knockdown of CIP2A reduced MYC protein levels without influencing MYC stability in CRC cell lines. Interfering with proteasomal degradation of MYC by usage of FBXW7-deficient cells or treatment with the proteasome inhibitor MG132 did not rescue the effect of CIP2A depletion on MYC protein levels. Whereas CIP2A knockdown had marginal influence on global protein synthesis, we could demonstrate that, by using different reporter constructs and cells expressing MYC mRNA with or without flanking UTR, CIP2A regulates MYC translation. This interaction is mainly conducted by the MYC 5′UTR.
Conclusions
Thus, instead of targeting MYC protein stability as reported for other tissue types before, CIP2A specifically regulates MYC mRNA translation in CRC but has only slight effects on global mRNA translation. In conclusion, we propose as novel mechanism that CIP2A regulates MYC on a translational level rather than affecting MYC protein stability in CRC.
Antibody-based soluble and membrane-bound TWEAK mimicking agonists with FcγR-independent activity
(2023)
Fibroblast growth factor (FGF)-inducible 14 (Fn14) activates the classical and alternative NFκB (nuclear factor ‘kappa-light-chain-enhancer’ of activated B-cells) signaling pathway but also enhances tumor necrosis factor (TNF)-induced cell death. Fn14 expression is upregulated in non-hematopoietic cells during tissue injury and is also often highly expressed in solid cancers. In view of the latter, there were and are considerable preclinical efforts to target Fn14 for tumor therapy, either by exploiting Fn14 as a target for antibodies with cytotoxic activity (e.g. antibody-dependent cellular cytotoxicity (ADCC)-inducing IgG variants, antibody drug conjugates) or by blocking antibodies with the aim to interfere with protumoral Fn14 activities. Noteworthy, there are yet no attempts to target Fn14 with agonistic Fc effector function silenced antibodies to unleash the proinflammatory and cell death-enhancing activities of this receptor for tumor therapy. This is certainly not at least due to the fact that anti-Fn14 antibodies only act as effective agonists when they are presented bound to Fcγ receptors (FcγR). Thus, there are so far no antibodies that robustly and selectively engage Fn14 signaling without triggering unwanted FcγR-mediated activities. In this study, we investigated a panel of variants of the anti-Fn14 antibody 18D1 of different valencies and domain architectures with respect to their inherent FcγR-independent ability to trigger Fn14-associated signaling pathways. In contrast to conventional 18D1, the majority of 18D1 antibody variants with four or more Fn14 binding sites displayed a strong ability to trigger the alternative NFκB pathway and to enhance TNF-induced cell death and therefore resemble in their activity soluble (TNF)-like weak inducer of apoptosis (TWEAK), one form of the natural occurring ligand of Fn14. Noteworthy, activation of the classical NFκB pathway, which naturally is predominately triggered by membrane-bound TWEAK but not soluble TWEAK, was preferentially observed with a subset of constructs containing Fn14 binding sites at opposing sites of the IgG scaffold, e.g. IgG1-scFv fusion proteins. A superior ability of IgG1-scFv fusion proteins to trigger classical NFκB signaling was also observed with the anti-Fn14 antibody PDL192 suggesting that we identified generic structures for Fn14 antibody variants mimicking soluble and membrane-bound TWEAK.
TNF-like weak inducer of apoptosis (TWEAK) and inhibition of protein synthesis with cycloheximide (CHX) sensitize for poly(I:C)-induced cell death. Notably, although CHX preferentially enhanced poly(I:C)-induced apoptosis, TWEAK enhanced primarily poly(I:C)-induced necroptosis. Both sensitizers of poly(I:C)-induced cell death, however, showed no major effect on proinflammatory poly(I:C) signaling. Analysis of a panel of HeLa-RIPK3 variants lacking TRADD, RIPK1, FADD, or caspase-8 expression revealed furthermore similarities and differences in the way how poly(I:C)/TWEAK, TNF, and TRAIL utilize these molecules for signaling. RIPK1 turned out to be essential for poly(I:C)/TWEAK-induced caspase-8-mediated apoptosis but was dispensable for this response in TNF and TRAIL signaling. TRADD-RIPK1-double deficiency differentially affected poly(I:C)-triggered gene induction but abrogated gene induction by TNF completely. FADD deficiency abrogated TRAIL- but not TNF- and poly(I:C)-induced necroptosis, whereas TRADD elicited protective activity against all three death inducers. A general protective activity against poly(I:C)-, TRAIL-, and TNF-induced cell death was also observed in FLIPL and FLIPS transfectrants.
Eine gefürchtete Komplikation nach Resektionen am Kolon mit Wiederherstellung der Kontinuität ist das Auftreten einer Anastomoseninsuffizienz (AI). Der Prozess der Diagnosestellung und das therapeutische Vorgehen sind zentrumsspezifisch und sehr heterogen. Ziel dieser Promotionsarbeit war die deskriptive Darstellung der Prävalenz, Diagnostik und Therapie von AI, um anhand dieser Daten ein bestimmtes zu favorisierendes Vorgehen zur AI-Behandlung herauszuarbeiten.
Es wurde eine retrospektive unizentrische Analyse durchgeführt. Diese umfasste eine Kohorte von 744 Patienten, welche von 2009 bis 2013 am Universitätsklinikum Würzburg unter Kontinuitätserhalt kolorektal reseziert wurden. Es erfolgte eine deskriptive und statistische Auswertung mittels uni- und multivariater Analysen in Bezug auf Demographie, Risikofaktoren für die Entwicklung einer AI und den Erfolg der einzelnen Therapiekonzepte.
Während der Nachbeobachtungsphase vom im Mittel 2,5 Jahren entwickelten 10,48% der Patienten eine AI. 60% der Insuffizienzen wurden während der ersten 7 postoperativen Tage detektiert. Als Risikofaktoren konnten indikationsunabhängig männliches Geschlecht, offener Zugangsweg und pulmonale Erkrankungen herausgearbeitet werden. Indikationsspezifisch zeigte sich eine Zunahme des AI-Risikos bei Divertikulitis-Patienten mit pulmonalen Erkrankungen (OR 4,5) und Cortisoneinnahme (OR 5,4). Auffällig wurden Patienten mit AI durch heterogene und teils unspezifische Symptome – am häufigsten durch Fieber (28,21%) und auffällige Laborwerte (48,72%). Eine folgende CT-Diagnostik bestätigte die Diagnose in 76,32% der Fälle und war in 24,48% falsch negativ. Patienten mit schlechtem AZ bei Diagnose der AI zeigten eine signifikant höhere Mortalität. Ein protektives Stoma konnte eine AI nicht verhindern, aber ihre Symptome und die Schwere des Verlaufs abmildern. Gemessen an der Überlebensrate und der Revisionspflichtigkeit unterschieden sich die durchgeführten Maßnahmen beim Versuch der kontinuitätserhaltenden Therapie nicht in Bezug auf den Erfolg der Therapie.
Wie Insuffizienz- und Mortalitätsrate nach AI zeigen, ist diese unizentrische Analyse international vergleichbar. Die Ableitung einer generellen Empfehlung zur therapeutischen Vorgehensweise bei AI ist nicht möglich. Vielmehr sind alle dargestellten Maßnahmen zur Beherrschung der AI sinnvoll, während die Wahl der Vorgehensweise weiterhin eine Individualentscheidung bleibt.
Background
With the emergence of photon-counting CT, ultrahigh-resolution (UHR) imaging can be performed without dose penalty. This study aims to directly compare the image quality of UHR and standard resolution (SR) scan mode in femoral artery angiographies.
Methods
After establishing continuous extracorporeal perfusion in four fresh-frozen cadaveric specimens, photon-counting CT angiographies were performed with a radiation dose of 5 mGy and tube voltage of 120 kV in both SR and UHR mode. Images were reconstructed with dedicated convolution kernels (soft: Body-vascular (Bv)48; sharp: Bv60; ultrasharp: Bv76). Six radiologists evaluated the image quality by means of a pairwise forced-choice comparison tool. Kendall’s concordance coefficient (W) was calculated to quantify interrater agreement. Image quality was further assessed by measuring intraluminal attenuation and image noise as well as by calculating signal-to-noise ratio (SNR) and contrast-to-noise ratios (CNR).
Results
UHR yielded lower noise than SR for identical reconstructions with kernels ≥ Bv60 (p < 0.001). UHR scans exhibited lower intraluminal attenuation compared to SR (Bv60: 406.4 ± 25.1 versus 418.1 ± 30.1 HU; p < 0.001). Irrespective of scan mode, SNR and CNR decreased while noise increased with sharper kernels but UHR scans were objectively superior to SR nonetheless (Bv60: SNR 25.9 ± 6.4 versus 20.9 ± 5.3; CNR 22.7 ± 5.8 versus 18.4 ± 4.8; p < 0.001). Notably, UHR scans were preferred in subjective assessment when images were reconstructed with the ultrasharp Bv76 kernel, whereas SR was rated superior for Bv60. Interrater agreement was high (W = 0.935).
Conclusions
Combinations of UHR scan mode and ultrasharp convolution kernel are able to exploit the full image quality potential in photon-counting CT angiography of the femoral arteries.
Relevance statement
The UHR scan mode offers improved image quality and may increase diagnostic accuracy in CT angiography of the peripheral arterial runoff when optimized reconstruction parameters are chosen.
Key points
• UHR photon-counting CT improves image quality in combination with ultrasharp convolution kernels.
• UHR datasets display lower image noise compared with identically reconstructed standard resolution scans.
• Scans in UHR mode show decreased intraluminal attenuation compared with standard resolution imaging.
Objectives
We developed a novel human cadaveric perfusion model with continuous extracorporeal femoral perfusion suitable for performing intra-individual comparison studies, training of interventional procedures and preclinical testing of endovascular devices. Objective of this study was to introduce the techniques and evaluate the feasibility for realistic computed tomography angiography (CTA), digital subtraction angiography (DSA) including vascular interventions, and intravascular ultrasound (IVUS).
Methods
The establishment of the extracorporeal perfusion was attempted using one formalin-fixed and five fresh-frozen human cadavers. In all specimens, the common femoral and popliteal arteries were prepared, introducer sheaths inserted, and perfusion established by a peristaltic pump. Subsequently, we performed CTA and bilateral DSA in five cadavers and IVUS on both legs of four donors. Examination time without unintentional interruption was measured both with and without non-contrast planning CT. Percutaneous transluminal angioplasty and stenting was performed by two interventional radiologists on nine extremities (five donors) using a broad spectrum of different intravascular devices.
Results
The perfusion of the upper leg arteries was successfully established in all fresh-frozen but not in the formalin-fixed cadaver. The experimental setup generated a stable circulation in each procedure (ten upper legs) for a period of more than six hours. Images acquired with CT, DSA and IVUS offered a realistic impression and enabled the sufficient visualization of all examined vessel segments. Arterial cannulating, percutaneous transluminal angioplasty as well as stent deployment were feasible in a way that is comparable to a vascular intervention in vivo. The perfusion model allowed for introduction and testing of previously not used devices.
Conclusions
The continuous femoral perfusion model can be established with moderate effort, works stable, and is utilizable for medical imaging of the peripheral arterial system using CTA, DSA and IVUS. Therefore, it appears suitable for research studies, developing skills in interventional procedures and testing of new or unfamiliar vascular devices.
Multiorgan recovery in a cadaver body using mild hypothermic ECMO treatment in a murine model
(2023)
Background
Transplant candidates on the waiting list are increasingly challenged by the lack of organs. Most of the organs can only be kept viable within very limited timeframes (e.g., mere 4–6 h for heart and lungs exposed to refrigeration temperatures ex vivo). Donation after circulatory death (DCD) using extracorporeal membrane oxygenation (ECMO) can significantly enlarge the donor pool, organ yield per donor, and shelf life. Nevertheless, clinical attempts to recover organs for transplantation after uncontrolled DCD are extremely complex and hardly reproducible. Therefore, as a preliminary strategy to fulfill this task, experimental protocols using feasible animal models are highly warranted. The primary aim of the study was to develop a model of ECMO-based cadaver organ recovery in mice. Our model mimics uncontrolled organ donation after an “out-of-hospital” sudden unexpected death with subsequent “in-hospital” cadaver management post-mortem. The secondary aim was to assess blood gas parameters, cardiac activity as well as overall organ state. The study protocol included post-mortem heparin–streptokinase administration 10 min after confirmed death induced by cervical dislocation under full anesthesia. After cannulation, veno-arterial ECMO (V–A ECMO) was started 1 h after death and continued for 2 h under mild hypothermic conditions followed by organ harvest. Pressure- and flow-controlled oxygenated blood-based reperfusion of a cadaver body was accompanied by blood gas analysis (BGA), electrocardiography, and histological evaluation of ischemia–reperfusion injury. For the first time, we designed and implemented, a not yet reported, miniaturized murine hemodialysis circuit for the treatment of severe hyperkalemia and metabolic acidosis post-mortem.
Results
BGA parameters confirmed profound ischemia typical for cadavers and incompatible with normal physiology, including extremely low blood pH, profound negative base excess, and enormously high levels of lactate. Two hours after ECMO implantation, blood pH values of a cadaver body restored from < 6.5 to 7.3 ± 0.05, pCO2 was lowered from > 130 to 41.7 ± 10.5 mmHg, sO2, base excess, and HCO3 were all elevated from below detection thresholds to 99.5 ± 0.6%, − 4 ± 6.2 and 22.0 ± 6.0 mmol/L, respectively (Student T test, p < 0.05). A substantial decrease in hyperlactatemia (from > 20 to 10.5 ± 1.7 mmol/L) and hyperkalemia (from > 9 to 6.9 ± 1.0 mmol/L) was observed when hemodialysis was implemented. On balance, the first signs of regained heart activity appeared on average 10 min after ECMO initiation without cardioplegia or any inotropic and vasopressor support. This was followed by restoration of myocardial contractility with a heart rate of up to 200 beats per minute (bpm) as detected by an electrocardiogram (ECG). Histological examinations revealed no evidence of heart injury 3 h post-mortem, whereas shock-specific morphological changes relevant to acute death and consequent cardiac/circulatory arrest were observed in the lungs, liver, and kidney of both control and ECMO-treated cadaver mice.
Conclusions
Thus, our model represents a promising approach to facilitate studying perspectives of cadaveric multiorgan recovery for transplantation. Moreover, it opens new possibilities for cadaver organ treatment to extend and potentiate donation and, hence, contribute to solving the organ shortage dilemma.
Loss of intestinal epithelial barrier function is a hallmark in digestive tract inflammation. The detailed mechanisms remain unclear due to the lack of suitable cell-based models in barrier research. Here we performed a detailed functional characterization of human intestinal organoid cultures under different conditions with the aim to suggest an optimized ex-vivo model to further analyse inflammation-induced intestinal epithelial barrier dysfunction. Differentiated Caco2 cells as a traditional model for intestinal epithelial barrier research displayed mature barrier functions which were reduced after challenge with cytomix (TNFα, IFN-γ, IL-1ß) to mimic inflammatory conditions. Human intestinal organoids grown in culture medium were highly proliferative, displayed high levels of LGR5 with overall low rates of intercellular adhesion and immature barrier function resembling conditions usually found in intestinal crypts. WNT-depletion resulted in the differentiation of intestinal organoids with reduced LGR5 levels and upregulation of markers representing the presence of all cell types present along the crypt-villus axis. This was paralleled by barrier maturation with junctional proteins regularly distributed at the cell borders. Application of cytomix in immature human intestinal organoid cultures resulted in reduced barrier function that was accompanied with cell fragmentation, cell death and overall loss of junctional proteins, demonstrating a high susceptibility of the organoid culture to inflammatory stimuli. In differentiated organoid cultures, cytomix induced a hierarchical sequence of changes beginning with loss of cell adhesion, redistribution of junctional proteins from the cell border, protein degradation which was accompanied by loss of epithelial barrier function. Cell viability was observed to decrease with time but was preserved when initial barrier changes were evident. In summary, differentiated intestinal organoid cultures represent an optimized human ex-vivo model which allows a comprehensive reflection to the situation observed in patients with intestinal inflammation. Our data suggest a hierarchical sequence of inflammation-induced intestinal barrier dysfunction starting with loss of intercellular adhesion, followed by redistribution and loss of junctional proteins resulting in reduced barrier function with consecutive epithelial death.
Background
Perioperative bridging of oral anticoagulation increases the risk of bleeding complications after elective general and visceral surgery. The aim of this study was to explore, whether an individual risk-adjusted bridging regimen can reduce bleeding events, while still protecting against thromboembolic events.
Methods
We performed a quality improvement study comparing bridging parameters and postoperative outcomes before (period 1) and after implementation (period 2) of a new risk-adjusted bridging regimen. The primary endpoint of the study was overall incidence of postoperative bleeding complications during 30 days postoperatively. Secondary endpoints were major postoperative bleeding, minor bleeding, thromboembolic events, postoperative red blood cell transfusion, perioperative length-of-stay (LOS) and in-hospital mortality.
Results
A total of 263 patients during period 1 and 271 patients during period 2 were compared. The included elective operations covered the entire field of general and visceral surgery. The overall incidence of bleeding complications declined from 22.1% during period 1 to 10.3% in period 2 (p < 0.001). This reduction affected both major as well as minor bleeding events (8.4% vs. 4.1%; p = 0.039; 13.7% vs. 6.3%; p = 0.004). The incidence of thromboembolic events remained low (0.8% vs. 1.1%). No changes in mortality or length-of-stay were observed.
Conclusion
It is important to balance the individual thromboembolic and bleeding risks in perioperative bridging management. The risk adjusted bridging regimen reduces bleeding events in general and visceral surgery while the risk of thromboembolism remains comparably low.
Es zeigte sich, dass die unmittelbare postoperative gesundheitsbezogene Lebensqualität erwartungsgemäß deutlich eingeschränkt, jedoch nach circa sechs Monaten wieder auf dem Ausgangsniveau der präoperativen Ebene angekommen war. Sowohl die Symptomskalen als auch die Funktionsskalen zeigten statistisch signifikante Unterschiede der erhobenen Werte bezüglich des Vergleichs der präoperativen zu den postoperativen Daten, dasselbe ließ sich über die Werte im Rahmen der Verlaufskontrolle nach circa sechs Monaten erheben. Eine kurzfristige Einbuße der Lebensqualität durch einen stationären Krankenhausaufenthalt sowie einer operativen Versorgung erscheint logisch. Für die zukünftige Entscheidung vor allem auch für Personen, welche aufgrund einer benignen Leberraumforderung eine operative Versorgung erhalten sollen, ist zu sagen, dass die globale gesundheitsbezogene Lebensqualität postoperativ nach circa sechs Monaten gleich bzw. etwas gebessert ausfiel und somit eine Rechtfertigung der operativen Versorgung auch bei benignen Erkrankungen darstellen kann.
Ein wesentlicher Aspekt der Arbeit ist, dass gezeigt werden konnte, dass auch bei komplexen Lebereingriffen eine schnelle Rekonvaleszenz - mindestens auf das Niveau vor dem Eingriff - innerhalb der ersten sechs Monate zu erwarten ist. Die systematische Erfassung der Lebensqualität hilft die postoperativen Einschränkungen und die Rekonvaleszenz zu normieren.
Ziele: Das Ziel dieser Dissertation ist es, die empirischen antibiotische Therapien (PAT) bei komplizierten intraabdominellen Infektionen (cIAI) in den Jahren 2016 – 2018 in einem großen deutschen Maximalversorger zu evaluieren. Aktuelle Studien legen nahe, dass viele Patienten keine Nachteile durch kürzere Therapien mit schmaler wirksamen Antibiotika oder das vermeiden einer nicht notwendigen antibiotischen Therapie haben.
Methoden: Es wurde eine retrospektive Kohortenstudie durch Analyse von elektronischen Patientenakten an einem 1500-Betten-Universitätsklinikum in Deutschland durchgeführt, bei der die Dauer der Antibiotikatherapie nach Notfalloperationen erhoben und mit antibiotischen Leitlinien durch die hausinterne Antibiotic-Stewardship-Abteilung (AMS) verglichen.
Ergebnisse: 767 Patienten konnten eingeschlossen werden, davon erhielten 404 (52.7%) eine PAT. Die Gesamtanzahl der Therapietage pro 100 Patiententagen ging von 47,0 auf 42,2 Tage zurück (p = 0,035) ohne einen Anstieg an Komplikationen. Patienten ohne Sepsis, bei denen eine initiale chirurgischer Fokuskontrolle möglich war profitierten nicht von einer Therapiedauer über 4 Tage (160 vs 100 Patienten). Bei Patienten, bei denen diese Bedingungen nicht gegeben waren, zeigte sich ebenfalls kein Vorteil bei längeren Behandlungen (über >7 Tage, 74 lang vs. 32 kurz behandelte Patienten). Es zeigte sich ebenfalls kein Vorteil von empirischen Therapien mit Carbapenem statt mit Piperacillin-Tazobactam (n=51 C vs n=40 vs Pip/Taz).
Schlussfolgerung: Die Reduktion unnötiger, zu breiter und zu langer antibiotischer Therapien bei cIAI ist ohne einen Anstieg der postoperativen Komplikationen möglich. Weitere RCTs sind notwendig, um das Wissen um sichere Behandlungen zu vergrößern.
This study evaluated the influence of different vascular reconstruction kernels on the image quality of CT angiographies of the lower extremity runoff using a 1st-generation photon-counting-detector CT (PCD-CT) compared with dose-matched examinations on a 3rd-generation energy-integrating-detector CT (EID-CT). Inducing continuous extracorporeal perfusion in a human cadaveric model, we performed CT angiographies of eight upper leg arterial runoffs with radiation dose-equivalent 120 kVp acquisition protocols (CTDIvol 5 mGy). Reconstructions were executed with different vascular kernels, matching the individual modulation transfer functions between scanners. Signal-to-noise-ratios (SNR) and contrast-to-noise-ratios (CNR) were computed to assess objective image quality. Six radiologists evaluated image quality subjectively using a forced-choice pairwise comparison tool. Interrater agreement was determined by calculating Kendall’s concordance coefficient (W). The intraluminal attenuation of PCD-CT images was significantly higher than of EID-CT (414.7 ± 27.3 HU vs. 329.3 ± 24.5 HU; p < 0.001). Using comparable kernels, image noise with PCD-CT was significantly lower than with EID-CT (p ≤ 0.044). Correspondingly, SNR and CNR were approximately twofold higher for PCD-CT (p < 0.001). Increasing the spatial frequency for PCD-CT reconstructions by one level resulted in similar metrics compared to EID-CT (CNRfat; EID-CT Bv49: 21.7 ± 3.7 versus PCD-CT Bv60: 21.4 ± 3.5). Overall image quality of PCD-CTA achieved ratings superior to EID-CTA irrespective of the used reconstruction kernels (best: PCD-CT Bv60; worst: EID-CT Bv40; p < 0.001). Interrater agreement was good (W = 0.78). Concluding, PCD-CT offers superior intraluminal attenuation, SNR, and CNR compared to EID-CT in angiographies of the upper leg arterial runoff. Combined with improved subjective image quality, PCD-CT facilitates the use of sharper convolution kernels and ultimately bears the potential of improved vascular structure assessability.
Die Erkrankung MC zählt zusammen mit der Colitis Ulcerosa zu den CED. In Deutschland liegt die Prävalenz bei ca. 100-200 Personen pro 100000 Einwohner und steigt stetig. Überwiegend sind Menschen im jungen Erwachsenenalter betroffen, die fest im Berufsleben stehen. Die Erkrankung führt im Verlauf immer wieder zu Arbeitsausfällen und verursacht neben den gesundheitlichen Kosten für Medikamente und Therapie auch wirtschaftliche Ausfälle. Trotz der hohen sozioökonomischen Bedeutung und des Vorliegens gesicherter Erkenntnisse zu Risikofaktoren, anatomischen / histologischen Veränderungen, Symptomkomplexen und zahlreicher Hypothesen bezüglich der Entstehung, ist die Pathogenese nicht gänzlich verstanden. Ebenso komplex wie das Erkrankungsbild selbst ist der Prozess der Diagnosefindung. Ein Goldstandard ist nicht etabliert. Die Diagnose MC ist meist eine klinische, in Zusammenschau mit endoskopischen, histologischen, laborchemischen und radiologischen Befunden. In dieser Arbeit wurde die Versorgungsrealität der MC Erkrankten über die letzten 15 Jahre betrachtet. Es konnte ein Wandel in der chirurgischen Operations- und Anastomosentechnik gezeigt werden. Die Zukunft ist eine minimalinvasive und darmsparende Chirurgie. Im retrospektiven Vergleich der Therapiealgorithmen erfolgte nach damaliger S3-Leitlinie in der Mehrzahl der MC Fälle initial eine medikamentöse Therapie, alle untersuchten Fälle erhielten jedoch eine ICR. In der Subgruppenanalyse wurden Fälle mit isolierter Ileitis terminalis Crohn untersucht. Es konnte die Effektivität der chirurgischen Primärtherapie gegenüber einer medikamentösen Primärtherapie gezeigt werden, was die Daten der aktuellen Literatur stützt. So bestand ein Vorteil hinsichtlich des verringerten Bedarfs an einer medikamentösen Therapie im postoperativen Verlauf von zwei Jahren und bezüglich der rezidiv- und medikamentenfreien Zeit. Die Ergebnisse zeigten zudem, dass eine präventive, postoperative medikamentöse Therapie bei präoperativ vorliegenden Risikofaktoren für ein klinisches Rezidiv nicht zwingend notwendig ist und überdacht werden sollte. Diese Arbeit konnte den Stellenwert der Chirurgie als wichtige Säule der Therapie bei isolierter Ileitis terminalis Crohn untermauern.
Brain μ-opioid receptors (MORs) stimulate high-fat (HF) feeding and have been implicated in the distinct long term outcomes on body weight of bariatric surgery and dieting. Whether alterations in fat appetite specifically following these disparate weight loss interventions relate to changes in brain MOR signaling is unknown. To address this issue, diet-induced obese male rats underwent either Roux-en-Y gastric bypass (RYGB) or sham surgeries. Postoperatively, animals were placed on a two-choice diet consisting of low-fat (LF) and HF food and sham-operated rats were further split into ad libitum fed (Sham-LF/HF) and body weight-matched (Sham-BWM) to RYGB groups. An additional set of sham-operated rats always only on a LF diet (Sham-LF) served as lean controls, making four experimental groups in total. Corresponding to a stage of weight loss maintenance for RYGB rats, two-bottle fat preference tests in conjunction with small-animal positron emission tomography (PET) imaging studies with the selective MOR radioligand [\(^{11}\)C]carfentanil were performed. Brains were subsequently collected and MOR protein levels in the hypothalamus, striatum, prefrontal cortex and orbitofrontal cortex were analyzed by Western Blot. We found that only the RYGB group presented with intervention-specific changes: having markedly suppressed intake and preference for high concentration fat emulsions, a widespread reduction in [\(^{11}\)C]carfentanil binding potential (reflecting MOR availability) in various brain regions, and a downregulation of striatal and prefrontal MOR protein levels compared to the remaining groups. These findings suggest that the suppressed fat appetite caused by RYGB surgery is due to reduced brain MOR signaling, which may contribute to sustained weight loss unlike the case for dieting.
Ureaplasma species (spp.) are considered commensals of the adult genitourinary tract, but have been associated with chorioamnionitis, preterm birth, and invasive infections in neonates, including meningitis. Data on mechanisms involved in Ureaplasma-driven neuroinflammation are scarce. The present study addressed brain inflammatory responses in preterm lambs exposed to Ureaplasma parvum (UP) in utero. 7 days after intra-amniotic injection of UP (n = 10) or saline (n = 11), lambs were surgically delivered at gestational day 128–129. Expression of inflammatory markers was assessed in different brain regions using qRT-PCR and in cerebrospinal fluid (CSF) by multiplex immunoassay. CSF was analyzed for UP presence using ureB-based real-time PCR, and MRI scans documented cerebral white matter area and cortical folding. Cerebral tissue levels of atypical chemokine receptor (ACKR) 3, caspases 1-like, 2, 7, and C–X–C chemokine receptor (CXCR) 4 mRNA, as well as CSF interleukin-8 protein concentrations were significantly increased in UP-exposed lambs. UP presence in CSF was confirmed in one animal. Cortical folding and white matter area did not differ among groups. The present study confirms a role of caspases and the transmembrane receptors ACKR3 and CXCR4 in Ureaplasma-driven neuroinflammation. Enhanced caspase 1-like, 2, and 7 expression may reflect cell death. Increased ACKR3 and CXCR4 expression has been associated with inflammatory central nervous system (CNS) diseases and impaired blood–brain barrier function. According to these data and previous in vitro findings from our group, we speculate that Ureaplasma-induced caspase and receptor responses affect CNS barrier properties and thus facilitate neuroinflammation.
Endoscopic management of umbilical and incisional hernias has adapted to the limitations of conventional laparoscopic instruments over the past 30 years. This includes the development of meshes for intraperitoneal placement (intraperitoneal onlay mesh, IPOM), with antiadhesive coatings; however, adhesions do occur in a significant proportion of these patients. Minimally invasive procedures result in fewer perioperative complications, but with a slightly higher recurrence rate. With the ergonomic resources of robotics, which offers angled instruments, it is now possible to implant meshes in a minimally invasively manner in different abdominal wall layers while achieving morphologic and functional reconstruction of the abdominal wall. This video article presents the treatment of ventral and incisional hernias with mesh implantation into the preperitoneal space (robot-assisted transabdominal preperitoneal ventral hernia repair, r‑ventral TAPP) as well as into the retrorectus space (r-Rives and robotic transabdominal retromuscular umbilical prosthetic repair, r‑TARUP, respectively). The results of a cohort study of 118 consecutive patients are presented and discussed with regard to the added value of the robotic technique in extraperitoneal mesh implantation and in the training of residents.
The surgical treatment of parastomal hernias is considered complex and is known to be prone to complications. Traditionally, this condition was treated using relocation techniques or local suture repairs. Since then, several mesh-based techniques have been proposed and are nowadays used in minimally invasive surgery. Since the introduction of robot-assisted surgery to the field of abdominal wall surgery, several adaptations to these techniques have been made, which may significantly improve patient outcomes. In this contribution, we provide an overview of available techniques in robot-assisted parastomal hernia repair. Technical considerations and preliminary results of robot-assisted modified Sugarbaker repair, robot-assisted Pauli technique, and minimally invasive use of a funnel-shaped mesh in the treatment of parastomal hernias are presented. Furthermore, challenges in robot-assisted ileal conduit parastomal hernia repair are discussed. These techniques are illustrated by photographic and video material. Besides providing a comprehensive overview of robot-assisted parastomal hernia repair, this article focuses on the specific advantages of robot-assisted techniques in the treatment of this condition.