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- D-1221-2009 (1)
Motoneuron diseases represent a m&jor challenge to modern neurology, yet their clinical manifestations ware first described more than hundred years ago, and despite many studies the etiology of these diseases ramd,ns obscure with no effective treatments having been reported. Although progress has been made in establishing genetic linkage in the rare inherited for.ms of these diseases such as familial amyotrophic lateral scleriosisl , spinal mDscular atrophy and X-linked bulbo-spinal-mDscular atrophy, this new information has not yet affected therapeutic techniques. During the last few years several important steps have been taken concerning the physiological mechanisms involved in motoneuron survival during development, after lesion and in animal models of degenerative diseases, the molecular clOning of several new neurotrophic factors (brain-derived neurotrophic factor (BDNP), neurotrophin-3 and-4 (NT-3 and NT-4) and ciliary neurotrophic factor (CNTP)); the identification of a gene family of receptor molecules for same of these factors, progress in the understanding of the effects of polypeptide growth factors on muscle cell differentiation, neuronal sprouting (insulin-like growth factor-I and -11 (IGF-I and IGF-II), and in vitro motoneuronal survival (CNTF, IGF-I and -II and basic FGF). These findings have raised new hopes in that they could lead to a better understanding of the pathophysiological processes underlying these diseases, and that the pharmacological use of same of these newly characterized neurotrophic factors could present new possibilities for the treatment of these diseases.
An Metabasiten der Zone Erbendorf-Vohenstrauß, der Erbendorfer Grünschieferzone und der Zone Tirschenreuth-Mähring wurden petrographische, geochemische und phasenpetrologische Untersuchungen sowie K-Ar-Mineraldatierungen durchgeführt. In der Zone Erbendorf-Vohenstrauß treten in regionaler Abhängigkeit drei Haupttypen von Metabasiten auf: 1. Flaseramphibolite mit der Paragenese Hornblende + Oligoklas/ Andesin + Granat ± Salit und mit Plagioklas- und / oder Kalksilikat-reichen Flasern findet man hauptsächlich im nördlichen und zentralen Teil der Zone Erbendorf-Vohenstrauß. Geochemisch zeigen sie eine deutliche Anreicherung der inkompatiblen Elemente und der leichten Seltenerdelemente und sind vergleichbar mit modernen Tholeiiten von ozeanischen Inseln oder anomalen Abschnitten mittelozeanischer Rükken. Ein kontinentaler Intraplatten-Charakter scheint nicht gegeben. In der Kontaktaureole von Windisch-Eschenbach wurde ein Teil der Flaseramphibolite kontaktmetamorph überprägt. 2. Schiefrige, teils streifige Amphibolite mit der Paragenese Hornblende + Andesin / Labrador ± Salit dominieren im Süd teil der Zone Erbendorf-Vohenstrauß, erscheinen aber in einzelnen Ausstrichen auch im Norden. Die Streifung entsteht durch Plagioklas- und / oder Kalksilikat-reiche Zwischenlagen. Geochemisch sind diese Amphibolite bei flachen Spurenelement- und Seltenerdelement- Mustern vergleichbar mit modernen Tholeiiten von normalen Abschnitten mittelozeanischer Rücken. Sowohl Flaseramphibolite als auch schiefrige, teils streifige Amphibolite zeigen Übergänge zu massigen, homogenen Amphiboliten. 3. Metagabbros mit der Paragenese Hornblende + Plagioklas ± Biotit und einer grobkörnigen, gabbro iden Textur treten hauptsächlich im Bereich der Schuppenzone Michldorf-Kaimling (VOLL 1960) auf. Geochemisch nehmen sie bei einer schwachen Anreicherung der inkompatiblen Elemente eine Zwischenstellung zwischen Flaseramphiboliten und schiefrigen, teils streifigen Amphiboliten ein. In der Erbendorfer Grünschieferzone treten Metagabbros und schiefrige, teils gestreifte Amphibolite mit der Paragenese Hornblende + Oligoklas ± Epidot ± Chlorit auf. Die Amphibolite wurden z. T. stark retrograd überprägt und dabei auch deformiert. Dies wird auf größere, post-regionalmetamorphe, tektonische Bewegungen, möglicherweise Deckenüberschiebungen zurückgeführt. Geochemisch sind die Metabasite der Erbendorfer Grünschieferzone mit modernen tholeiitischen bis kalkalkalischen, subduktionsgebundenen Basalten vergleichbar. Sie unterscheiden sich damit völlig von den südlich angrenzenden Flaseramphiboliten der Zone Erbendorf-Vohenstrauß und den nördlich benachbarten Amphiboliten des Fichtelgebirges. Dies unterstreicht die Stellung der Erbendorfer Grünschieferzone als eigenständige tektonische Einheit. Im Vergleich mit dem Münchberger Komplex zeigt die Erbendorfer Grünschieferzone große Ähnlichkeit mit der Prasinit-Phyllit-Serie hinsichtlich der tektonischen Stellung, der Lithologie und insbesondere des geochemischen Charakters der Metabasite. Zwischen der Zone Erbendorf-Vohen-strauß und den Münchberger Serien konnten bezüglich der Metabasite dagegen keine Parallelen festgestellt werden. Schiefrige, teils streifige Amphibolite der Zone Tirschenreuth-Mähring mit der Paragenese Hornblende + Andesin ± Salit (± Granat) und hellen Plagioklas- oder Kalksilikat-reichen Lagen sind geochemisch mit modernen Basalten normaler mittel ozeanischer Rücken vergleichbar. Die untersuchten Metabasite dürften die Zusammensetzung des basaltischen Ausgangsmaterials zumindest annähernd wiedergeben. In einigen Fällen wurde jedoch eine sekundäre Mobilisation von Elementen festgestellt: Die kontaktmetamorphe Überprägung eines Teils der Flaseramphibolite in der Kontaktaureole von Windisch-Eschenbach führte zu einer deutlichen Anreicherung von Rb, Li und K und zu einer Abreicherung von Ca, Sr und V, insbesondere aber von Cr und Ni. Die sog. immobilen Elemente Nb, Ce, (P), Zr, Ti, Y, Sc streuen in einem deutlich breiteren Bereich als bei den nicht kontaktmetamorphen Flaseramphiboliten. In einer Probe sind die Seltenerdelemente insgesamt angereichert. Eine Mobilisation von P20 S und den leichten Seltenerdelementen in einigen schiefrigen, teils streifigen Amphiboliten der Zone Erbendorf-Vohenstrauß und in einigen kontaktmetamorphen Flaseramphiboliten wird auf einen Abbau von Apatit im Zuge einer post-regionalmetamorphen, möglicherweise postgranitischen, hydrothermalen Überprägung zurückgeführt. Mineralchemische Untersuchungen ergaben für die Zusammensetzung der Hornblenden aus Amphiboliten der Zone Erbendorf-Vohenstrauß deutliche regionale Unterschiede, die weitgehend unabhängig von der regionalen Verteilung der einzelnen Metabasit-Haupttypen sind. Vergleichende Untersuchungen an Granat untermauern eine vermutete, frühere, eklogitfazielle Überprägung eines Granatamphibolits vom Nordrand der Zone Erbendorf-Vohenstrauß bei Hauxdorf. K-Ar-Altersdatierungen an Hornblende- und Glimmerkonzentraten belegen, daß das letzte, (Mitteldruck-) metamorphe Ereignis im Westteil der Zone Erbendorf-Vohenstrauß vor etwa 380 Ma beendet war. Dieses Alter wird korreliert mit einer letzten Metamorphose der höhermetamorphen Serien des Münchberger Komplexes zur gleichen Zeit. Im Ostteil der Zone Erbendorf-Vohenstrauß treten jüngere Altersdaten mit einem Schwerpunkt bei 324 Ma auf, die entweder auf einen Einfluß der etwa gleichalten Niederdruck-Metamorphose im unmittelbar benachbarten Moldanubikum i. e. S. oder auf die Intrusion des 324 Ma alten (KÖHLER et al. 1974) Leuchtenberger Granits zurückzuführen sind. Im Übergangsbereich vom Saxothuringikum zum Moldanubikum bei Mähring zeigen Altersdaten um 320Ma das Ende der letzten (Niederdruck-)Metamorphose an.
Nonnucleolar chromatin from interphase nuclei of Physarum polycephalum plasmodia occurs in two different structural configurations as seen in electron microscopic spread preparations. While the majority of the chromatin is devoid of nascent ribonucleoprotein (RNP) fibrils and compacted into nucleosomal particles, a minor proportion (10- 20%) is organized differently and reveals a smooth contour. It is this form of smooth chromatin which is rich in transcription units (mean length: 1.36±0.21 11m). Only occasionally are solitary nascent RNP fibrils observed which are associated with beaded strands of chromatin. In transcribed smooth chromatin nucleosomal particles are not only absent from the transcription units but also from their nontranscribed flan king regions, indicating that this special structural aspect is not merely a direct consequence of the transcriptional process. The existence of ca. 10- 20% of Physarum chromatin in the smoothly contoured form is discussed in relation to reports of a preferential digestibility of a similar proportion of Physarum chromatin by DNAse I (Jalouzot et al. , 1980) and to the altered configuration of "peak A" chromatin subunits after micrococcal nuclease digestion (Johnson et al., 1978a, b).
Oocytes of the water beetle, Dytiscus marginalis, contain large amounts of rDNA most of which is present in the form of rings containing one or several pre-rRNA genes. Electron microscopy of spread preparations of vitellogenic oocytes has shown that the rDNA is extended in chromatin rings with transcribed pre- rRNA genes and is not packed into nucleosomes (Trendelenburg eta!. , 1976). When similar preparations are made from previtellogenic ooytes in which a large proportion of the nuc1eolar chromatin is transcriptionally inactive, a different morphological form of this chromatin is recognized. In contrast to the transcribed chromatin rings the inactive nucleolar chromatin circles show the characteristic beaded configuration, indicative of nucleosomal packing. Nuc1eosomal packing is also indicated by the comparison of the lengths of these chromatin rings with both iso lated rDNA circ1es and transcribed chromatin rings. In addition, these inactive nuc1eofilaments often appear to be compacted into globular higher order structures of diameters from 21 to 34nm, each composed of an aggregate of 6-9 nuc1eosomes. While the estimated reduction of the overall length of rDNA, as seen in our preparations, is, on the average, only 2.2 - 2.4 fold in the nuc1eosomal state it is 10- 13 fold when supranuc1eosomal globules are present. These data show that the extrachromosomal rDNA of these oocytes goes through a cycle of condensation and extensio n, as a function of the specific transcriptional activity, and that the beaded state described here is exc1usively found in the non-transcribed state.
Lengths and patterns of transcriptional units in the amplified nucleoli of oocytes of Xenopus laevis
(1977)
Transcriptionally active chromatin from peripheral amplified nuc1eoli of lampbrush-chromosome stage oocytes of Xenopus laevis was dispersed and spread in various solutions of low salt concentrations (incIuding some with additions of detergents) and examined by electron microscopy. Nucleolar material from oocytes of animals with normal (2-nu) and mutant (I-nu) genetical constitution of nucleolus organizers was compared. Histograms showing the distributions of the lengths of matrix units, apparent spacer intercepts, and the total repeating units of the rDNA containing chromatin axes revealed a significant degree of heterogeneity, with indications of subclasses and predominant repeat unit size c1asses of 3.3 and 3.8 11m length. The correspondence of matrix unit length to the molecular weight of the first stable product of rDNA transcription was studied using gel electrophoresis of labelIed pre-rRNA under non-denaturing and denaturing conditions. Evaluations of individual strands of nucleolar chromatin furt her demonstrated the existence of both (i) strands with obviously homogeneous repeating units and (ii) strands with intra-axial heterogeneity of rDNA subunits. " Preludecomplexes ", i.e. groups of transcriptional complexes in apparent spacer intercepts, were not infrequently noted. The data are compared with the measurements of lengths of repeating units in fragments of rDNA obtained by digestion with EcoRI endonuclease as described by Morrow et al. (1974) and Wellauer et al. (1974, 1976a, b). The results are discussed in relation to problems of variations in the modes of arrangement of the pre-rRNA genes, the state of packing of rDNA during transcription, and possible mechanisms of the amplification process.
The Ringgold Knoll pegmatite, a late-stage member of the Granite Harbour Intrusives, crosscuts high-grade Wilson gneisses of the Oates Coast, which forms the westernmost part of the Wilson Terrane at the Pacific end of the Cambro-Ordovician Ross orogenic belt in West Antarctica. The pegmatite mineral assemblage consists of K-feldspar, plagioclase, quartz, garnet (almandinespessartine-pyrope), dark tourmaline (schorl-dravite), muscovite, apatite, monazite, zircon, blue AI-rich tourmaline and dumortierite in order of decreasing abundances. Major, minor and rare earth elements are reported for the greater part of the mineral assemblage. The time of pegmatite emplacement is constrained by Rb-Sr and Sm-Nd isochron ages of 492 ± 8 (2a) Ma and 500 ± 40 (2a) Ma, respectively. High initial 87Sr/86Sr of 0.7315 ± 0.0003 and low E Nd,t of -8.7 ± 1.2 strongly support an origin of the magma from highly evolved crustal source rocks. K-Ar and Ar-Ar model ages of about 470 to 475 Ma for igneous muscovite indicate that the pegmatite together with its wall rocks spent a prolonged period at elevated temperatures before final cooling below about 350 °C. The muscovite dates may give an estimate for the time of exhumation of the Oates Coast crystalline basement along two major late Ross orogenic detachment zones within the Wilson Terrane i.e. the Wilson and the Exiles thrusts (c.f. FLÖTTMANN and KLEINSCHMIDT, 1991).
The paleotropical tree genus Macaranga (Euphorbiaceae) comprises all stages of interaction with ants, from facultative associations to obligate myrmecophytes. In SE.-Asia food availability does not seem to be the limiting factor for the development of a close relationship since all species provide food for ants in form of extrafloral nectar and/or food bodies. Only myrmecophytic Macaranga species offer nesting space for ants (domatia) inside intern odes which become hollow due to degeneration of the pith. Non-myrmecophytic species have a solid stem with a compact and wet pith and many resin ducts. The stem interior of some transitional species remains solid, but the soft pith can be excavated. The role of different ant-attracting attributes for the development of obligate ant-plant interactions is discussed. In the genus Macaranga, the provision of nesting space seems to be the most important factor for the evolution of obligate myrmecophytism.
Nuclear envelopes of maturing oocytes of various amphibia contain an unusually high number of pore complexes in very close packing. Consequently, nuclear envelopes , which can be manually isolated in great purity, provide a remarkable enrichment of nuclear pore complex material, relative to membranous and other interporous structures. When the polypeptides of nuclear envelopes isolated from oocytes of Xenopl/s la evis and Triturus alpestris are examined by gel electrophoresis, visualized either by staining with Coomassie blue or by radiotluorography after in vitro reaction with [3H]dansyl chloride , a characteristic pattern is obtained (10 major and 15 minor bands). This polypeptide pattern is radically different from that of the nuclear contents isolated from the same cell. Extraction of the nuclear envelope with high salt concentrations and moderateIy ac tive detergents such as Triton X- 100 results in the removal of membrane material but leaves most of the non-membranous structure of the pore complexes. The dry weight of the pore complex (about 0.2 femtograms) remains essentially unchanged during such extractions as measured by quantitative electron microscopy . The extracted preparations which are highly enriched in nuclear pore complex material contain only two major polypeptide components with apparent molecular weights of 150000 and 73000. Components of such an electrophoretic mobility are not present as major bands , if at all , in nuclear contents extracted in the same way. lt is concluded that these two polypeptides are the major constituent protein(s) of the oocyte nuclear pore complex and are specific for this structure. When nuclear envelopes are isolated from rat liver and extracted with high salt buffers and Triton X- 100 similar bands are predominant, but two additional major components of molecular weights of 78000 and 66000 are also recognized. When the rat liver nuclear membranes are further subfractionated material enriched in the 66000 molecular weight component can be separated from the membrane material, indicating that this is relatively loosely associated material , probably a part of the nuclear matrix . The results suggest that the nuclear pore complex is not only a characteristic ubiquitous structure but also contains similar, if not identical , skeletal proteins that are remarkably re sistant to drastic changes of ionic strength as weil as to treatments with detergents and thiol reagents.
Sizes of chromosome loops and hnRNA molecules in oocytes of amphibia of different genome sizes
(1982)
The lengths of lampbrush chromosome loops and their tran scription units show a positive correlation with genome size in oocytes of amphibia with different C values. However, there is no such correlation with contour lengths of hnRNA molecu les isolated from these oocytes. These results indi cate th at more ON A sequences are transcribed in amphibia of higher C value , but that processing of RNA transc ripts occurs while they are still attached to the chromosomes as nascent ribonucleoprotein fibrils.