Refine
Has Fulltext
- yes (197)
Is part of the Bibliography
- yes (197)
Year of publication
- 1992 (197) (remove)
Document Type
- Journal article (144)
- Book article / Book chapter (23)
- Conference Proceeding (15)
- Book (8)
- Preprint (2)
- Review (2)
- Jahresbericht (1)
- Other (1)
- Report (1)
Language
- English (118)
- German (75)
- French (2)
- Portuguese (2)
Keywords
- Anorganische Chemie (16)
- Organische Chemie (8)
- Psychologie (8)
- Infektionsbiologie (7)
- Toxikologie (7)
- Physiologische Chemie (6)
- Biochemie (5)
- Aufsatzsammlung (4)
- Biologie (4)
- Neurobiologie (4)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (31)
- Institut für Psychologie (bis Sept. 2007) (25)
- Institut für Anorganische Chemie (24)
- Institut für Molekulare Infektionsbiologie (10)
- Institut für Organische Chemie (10)
- Institut für Pharmakologie und Toxikologie (8)
- Klinik und Poliklinik für Allgemein-, Viszeral-, Gefäß- und Kinderchirurgie (Chirurgische Klinik I) (8)
- Physikalisches Institut (8)
- Institut für Psychologie (6)
- Institut für Pädagogik (bis Sept. 2007) (6)
Schriftenreihe
Band 2 : Sprung, Wurf, Stoß
(1992)
No abstract available.
No abstract available.
No abstract available.
No abstract available.
No abstract available.
Single crystal X-ray studies on bis[3,4,5,6-tetrabromo-1 ,2-benzenediolato(2- )](pyrrolidiniomethyl)silicate acetonitrile solvate [(C6Br40 2hSiCH2(H)NC4H8 · CH3CN; monoclinic, P2t/c, a = 808.5(4), b = 1533.0(8), c = 2212.6(1) pm, ß = 97.67(2)0 , Z = 4] revealed a zwitterionic structure with a pentacoordinate, formally negatively charged silicon atom and a positively charged ammonium moiety. The silicon atom is surrounded by four oxygen atoms and one carbon atom in a trigonalbipyramidal fashion, with the carbon atom in an equatorial position. The structure is displaced by 7.0% from the trigonal bipyramid towards the square pyramid. The zwitterion and the CH3CN molecule form intermolecular N-H · · · N hydrogen bonds.
Efficient expression systems are required for analysis of gene regulation and function in teleost fish. To develop such systems, a nurober of inducible or constitutive promoter and enhancer sequences of fish or higher vertebrate origin were tested for activity in a variety of fish celllines andin embryos of the Japanese medaka fish (Oryzias latipes) and Xiphophorus. The activity of the different promoterenhancer combinations were quantitated. Considerable differences were found for some constructs if tested in vitro or in vivo. From the data obtained, a set of expression vectors for basic research as weH as for aquaculture purposes were established.
The most commonly used assay to quantitate the response of peripheral T~cells upon stimulation with growth factors is determination of incorporated (JH]TdR. We compared thls test to three other methods: 1. direct countlog of cells with a Coulter type counter as reference assay, 2. a colorimetric assay using the tetrazolium dye 3-[ 4,S-dimethylthiazol-l-yl]-2,5diphenyl tetrazolium (MTT), which is a cheap and increasingly popular non-radioactive method and 3. incorporation of the thymidine analog 5-bromo-2'-deoxyuridine detection with a monoclonal antibody on cytospins. Primary human PHA-blasts from >30 healthy individuals were stimulated with IL-2, IL-4 aod IL-7 and assayed with up to four different methods. We discuss the advantages and disadvantages of the assays used and tbe effects of differences between cell preparations. We observed no significant variations between individuals for the dose dependence, but the relative emctency of IL4 compared to IL-2 and IL-7 was variable. This was probably due to the slower response observed upon stimulation with this factor.
No abstract available
No abstract available.
Escherichia coli 0139: K82: H1 strains originating from outbreaks and single cases of oedema disease in pigs were characterized by their genomic restriction fragment length polymorphism (RFLP), their virulence pattern, and by the occurrence as well as the genomic distribution of the determinants for hemolysin (hly) and verotoxins (shiga-like toxins; sltI, sltII). Whereas the RFLPs revealed considerable variation among the E. coli 0139: K82: H1 isolates depending the origin and epidemic source of the strains, the virulence gene slt II was found to be present in nearly all strains in a particular chromosomal region. Similar to RFLPs, the plasmid profiles are useful for epidemiological analysis.
Alkohol: Konsum, Wirkung, Gefahren für die Verkehrssicherheit. Zur Diskussion um neue Grenzwerte
(1992)
Im ersten Teil werden die Ergebnisse einer umfangreichen Literaturstudie zu Auswirkungen niedriger Alkoholkonzentrationen auf die Fahrsicherheit vorgestellt. Es wird gezeigt, daß bei Konzentration über 0.6 Promille die Leistungsausfälle drastisch ansteigen, dies umso mehr, je mehr es sich um komplexe Handlungen handelt und je näher der Zeitpunkt der Leistungsabgabe dem Trinkende liegt. Diese Ergebnisse decken sich mit Studien aus Verkehrsunfällen, bei denen ebenfalls über 0.7 Promille ein explosionsartiges Ansteigen des Risikos gezeigt wurde. Demgegenüber ist ein deutlich erhöhtes Risiko für den Bereich unter 0.5 Promille nicht nachgewiesen. An Untersuchungen zum Alkoholkonsum wird aufgezeigt, daß die Alkoholtrinker in mehrere Subpopulationen aufzuteilen sind. Dabei wird insbesondere eine Gruppe von Konsumtrinkern identifiziert, deren BAK regelhaft 0.6/0.7 Promille nicht überschreitet. Es wird gezeigt, daß diese Gruppe wahrscheinlich nicht überproportional zu ihrem Auftreten an Unfällen beteiligt ist. Daraus wird die Forderung nach der Einführung eines einzigen, strafrechtlich bewehrten Grenzwerts abgeleitet, der bei etwa 0.7/0.8 Promille liegen sollte.
No abstract available.
No abstract available.
Zum eblaitischen Gott Kura
(1992)
No abstract available.
We have previously reported that in several renal cell types, adenosine receptor agonists inhibit adenylyl cyclase and activate phospholipase C via a pertussis toxin-sensitive G protein. In the present study, in 28A cells, both uf these adenosine receptor-mediated responses were inhibited by 8-cyclopentyl-1,3-dipropylxanthine (DPCPX). a highly selective A1 adenosine receptor antagonist. The binding characteristics of the adenosine A 1 receptor in the 28A renal cell line were studied using the radiolabeled antagonist f:1H]DPCPX to determine whether two separate binding sites could account for these responses. Saturation binding of [: 1H]DPCPX to 28A cell membranes revealed a single class of A1 binding sites with an apparent Kd value of 1.4 nM and maximal binding capacity of 64 fmol/mg protein. Competition experiments with a variety of adenosine agonists gave biphasic displacement curves with a pharmacological profile characteristic of A1 receptors. Comparison of [: 1H]DPCPX competition binding data from 28A cell membranes with rabbit brain membranes, a tissue with well-characterized A1 receptors, reveals that the A 1 receptor population in 28A cells has similar agonist binding affinities to the receptor population in brain but has a considerably lower density. Addition of guanosine ;)' -triphosphate ( 100 ,uM) to 28A cell membranes caused the competition curves to shift from biphasic to monophasic. indicating that the A1 receptors exist in two interconvertible affinity states because of their coupling to G proteins. In the absence of evidence for subpopulations of the A1 receptor, it appears that in 28A cells. A single A1 receptor population. As defined by ligand binding characteristics, couples via one or more pertussis toxin-sensitive guanine nucleotide binding proteins to two different biological signaling mechanisms.
No abstract available.
E. coli strain 536 (06: K15: H31) isolated from a case of acute pyelonephritis, expresses S-fimbrial adhesins, P-related fimbriae, common type I fimbriae, and hemolysins. The respective chromosomally encoded determinants were cloned by constructing a genomic library of this strain. Furthermore, the strain produces the iron uptake substance, enterocheline, damages HeLa cells, and behaves in a serum-resistant mode. Genetic analysis of spontaneously arising non-hemolytic variants revealed that some of the virulence genes were physically linked to large unstable DNA regions, termed "pathogenicity islands", which were mapped in the respective positions on the E. coli K-12linkage map. By comparing the wild type strain and mutants in in vitro and in vivo assays, virulence features have been evaluated. In addition, a regulatory cross talk between adhesin determinants was found for the wild-type isolate. This particular mode of virulence regulation is missing in the mutant strain.
In 55 chronic schizophrenics, the occurrence of infectious diseases during their mothers' pregnancies was investigated. Different psychiatrie diagnostic systems were compared. Infections were reported by the mothers of familial and sporadic DSM I1I-R schizophrenics in equal proportion. However, applying Leonhard's classification, the frequency of infections was found to be significantly increased in 'systematic' schizophrenia (mainly exogenously induced in the view of Leonhard) compared to 'unsystematic' schizophrenia (mainly genetically determined according to Leonhard's findings). Most of the infections occurred during the second trimester (nine out of 13). Thus, in the 'systematic' forms of schizophrenia (low genetic loading), maternal infections in this crucial period of neurodevelopment would appear to be important causative factors in the cytoarchitectural deviance detected in the central nervous system of schizophrenics.
Der Aufsatz beschreibt die portugiesischen Wörter arabischen Ursprungs, und zwar unter verschiedenen Gesichtspunkten: im Vergleich mit den Arabismen des Spanischen, Katalanischen und Italienischen; die Anzahl der portugiesischen Arabismen, ihre lexikalische Struktur, ihre Verteilung nach Sachbereichen und ihre geographische Verbreitung. Untersucht werden nur sichere und direkte Arabismen, die heute noch gebäuchlich sind. Einige Anmerkungen zum inneren Lehngut (Lehnübersetzung, Lehnbedeutung u.ä.) schließen den Artikel ab.
In one of the simplest metazoan organisms, the sponge Spongilla lacustris, at least four different src-related kin ase genes (srkl-4) are expressed, aD of which show a high degree of similarity to the c-src genes of vertebrates. Whereas srk2 and srk3 are c1early unrelated at the nucleic acid level, srkl and srk4 share identical sequences in the 5' parts of their cDNAs. The cloning of several primer extension clones and genomic polymerase chain re action experiments confirmed the hypo thesis of an alternative splicing of tandemly arranged carboxyterminal parts of srkl and srk4. The genomic sequence encoding both proteins was found to be interrupted at the splice point by an intron which is located in the same position as one of the introns in the chicken src gene, which is the only gene conserved in invertebrates and vertebrates. All four srk genes are expressed in adult sponges as mRNA transcripts of about 2.2 kb. Tyrosine kin ase activity of a src-related kin ase could be detected in adult sponges but not in their resting form (gemmulae), and may reflect the activity of the srk protein products. Spongilla lacustris is the simplest organism from which a pro tein tyrosine kinase gene has been isolated. The presence of at least four such genes in the evolutionary ancient and primitive phylum Porifera suggests that tyrosine kinase genes arose concomitantly with or shortly after the appearance of multicellular organisms and that their activity may be involved in aggregation and cell-cell recognition.
Da organische Ursachen für die Non-Ulcer Dyspepsia nicht nachweisbar sind und außerdem bekannt ist, daß die Psyche einen wichtigen Einfluß auf die Magenfu nktion hat. werden häufig psychologische Faktoren als Ursache der NUD angesehen. Bisher liegen empirische Arbeiten über die Psychopathologie und Persönlichkeitsstruktur der NUD-Patienten. deren Krankheitsverhalten sowie über den Zusammenhang zwischen Streß und Magenbeschwerden vor. Eine kritische Sichtung dieser Arbeiten ergab, daß umer den psychologischen Variablen die Angst und das Krankheitsverhalten der NUD-Patienten eine besondere Rolle zu spielen scheinen. In zukünftigen Studien sollte außerdem mehr als bisher auf die Abgrenzung gegenüber anderen funktionellen Störungsbildern und auf eine bessere Differenzierung (u. a. hinsichtlich physiologischer Funktionsveränderungen) innerhalb der heterogenen Gruppe der NUD-Patienten geachtet werden. Lohnenswert erscheint es auch, die bisher noch gar nicht untersuchten Personen mit Magenbeschwerden, aber ohne Arztkomakt. genauer psychologisch zu untersuchen.
Der Aufsatz beschäftigt sich mit der lautlichen Anpassung der arabischen Lehnwörter im Portugiesischen. Behandelt werden die Betonung, der Vokalismus und der Konsonantismus sowie verschiedene kontextabhängige Lautwandelerscheinungen.
Die vorliegende Arbeit eines personzentrierten Psychotherapeuten will zu einer einheitlichen Theorie von Psychotherapie und so "zum allmählichen Ableben der verschiedenen ‚Schulen‘ der Psychotherapie" (Rogers, 1961) beitragen. Schulgebundene Begriffe erscheinen nach dem "Prinzip der Aspektivität und Komplementarität" als "standortbedingte Teilansichten eines gemeinsamen Gegenstandes" (Pongratz, 1975). Dies wird durch die Formulierung der Personzentrierten Psychotherapie als Therapeutisches Basisverhalten und Differenzielle Gesprächspsychotherapie verdeutlicht und deren Wirksamkeit durch experimentelle und ex-post-facto Forschung belegt.
The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca\(^{2+}\)·ionophore (A23187), or tumor necrosis factor (TNF) and interleukin·l (IL.l). Treatment ofcultured BMECs fron. WKY and SHR with all of these factors dose·dependently increased their total amount of TF; no substantive differences in the Ieveis of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.
Opioid peptidesandmultiple opioid receptors are found in brain cardiovascular nuclei, autonomic ganglia, the heart, and blood vessels, and opioids induce potent cardiovascular changes. The role of endogenaus opioids in normal cardiovascular homeostasis is unclear; however, current data suggest opioid involvement in stress.
Cardio-respiratory changes and mortality in the conscious rat induced by (+)- and (±)- anatoxin-a
(1992)
0. M. ADEYEMO and A.-L. SIREN. Cardio-respiratory changes and mortality in the conscious rat induced by ( + )- and ( ± )-anatoxin-a. Toxicon 30, 899-905, 1992.-Anatoxin-a (AnTx-a) isapotent nicotinic cholinergic receptor agonist. The relative potencies of the ( + )-AnTx-a and the racemic mixture ( ± )-AnTxa were investigated in the conscious rat by comparing their effects on mean arterial blood pressure (BP), heart rate (HR), blood oxygen and carbon dioxide pressures (p02 and pC02, respective1y), acid-base balance (pH) and mortality. The present experiments show that while both forms of AnTx-a produce dose-dependent increases in BP and decreases in HR, ( + )-AnTx-a is about IO-fo1d morepotent than the optically inactive isomer. ( + )-AnTx-a was also 6-fo1d more potent than ( ± )-AnTx-a in produclog severe hypoxemia, and more than 4-fold as potent as the (±}-AnTx-a in producing significant hypercapnia accompanied with severe acidosis. The approximate median Iethai dose (Ln so) of ( + )-AnTx-a was about 5-fold less than that of ( ± )-AnTx-a. We conclude that ( + )-AnTx-a is more potent than the ( ± )-AnTx-a racemic mixture in causing detrimental cardio-respiratory changes and therefore increased mortality in the rat.
The possibility that \(\mu\)Opioid-induced tachycardia and bradycardia could be mediated by different subtypes of the \(\mu\)·receptor was studied in conscious Sprague-Dawley rats. The selective \(\mu\)·receptor agonist dermorphin and its analog, TAPS (Tyr-o-Arg-Phe-sarcosine), a putative \(\mu _1\)-receptor agonist, were given centrally. Tyr-o-Arg-Phe-sarcosine increased the heart rate, the response being inversely correlated to the dose (an increase of 71 ± 22, 49 ± 14 and 30 ± 17 beats/min at doses of 0.3, 3 and 30 pmol, respectively). Dermorphin induced less clear changes in heart rate (maximum increase of 39 ± 14 beats/min at the dose of 1 pmol). Aftertreatment with the Jl 1-selective antagonist naloxonazine (NAZ), TAPS 30 pmol and dennorphin I pmol decreased heart rate by -22 ± 10 and -24 ± 7 bpm, respectively. The bradycardic effect oflarger doses of dennorphin was potentiated by NAZ (from -25 ± 8 to -97 ± 22 bpm) but abolished by the non-selective antagonist naloxone. These data suggest that the high affinity \(\mu _1\)-opioid receptors mediate tachycardic responses and \(\mu _2\)-receptors mediate bradycardic responses.