Refine
Has Fulltext
- yes (11)
Is part of the Bibliography
- yes (11)
Year of publication
Document Type
- Journal article (11)
Language
- English (11) (remove)
Keywords
- Aspergillus (2)
- COVID-19 (2)
- T cells (2)
- infectious diseases (2)
- invasive aspergillosis (2)
- Aspergillus fumigalus (1)
- Aspergillus fumigatus (1)
- B cell receptors (1)
- CAPA (1)
- CAR T cells (1)
- CMV (1)
- Candida albicans (1)
- HBV (1)
- HCV (1)
- HIV (1)
- Medizin (1)
- Pseudomonas aeruginosa (1)
- RNA extraction (1)
- Rhizopus (1)
- allogeneic stem cell transplantation (1)
- antimicrobial responses (1)
- aspergillus fumigatus (1)
- biofilm formation (1)
- biological fluorescence (1)
- carcinoma cells (1)
- cytokine production (1)
- dendritic cells (1)
- denritic cells (1)
- diagnostics (1)
- fluorescence imaging (1)
- fungal aerosolization (1)
- fungal infection (1)
- gene expression (1)
- gene expression data (1)
- gene regulation (1)
- granulocytes (1)
- human biomarker (1)
- human dendritic cells (1)
- imaging the immune system (1)
- immune impairment (1)
- immune receptors (1)
- immune response (1)
- immunogenetics (1)
- induced apoptosis (1)
- infection (1)
- mAb engineering (1)
- metabolic modelling (1)
- mouse model (1)
- oxidative stress (1)
- porcine large animal model (1)
- pulmonary immune response (1)
- signalling (1)
- super-resolution microscopy (1)
- systemic candidiasis (1)
Institute
EU-Project number / Contract (GA) number
- 847507 (1)
Early onset sepsis due to group B streptococcus leads to neonatal morbidity, increased mortality, and long-term neurological deficencies. Interaction between septicemic GBS and confluent monolayers of human coronary artery endothelial cells (HCAECs) was analyzed by genome wide expression profiling. In total, 124 genes were differentially expressed (89 upregulated, 35 downregulated) based on a more than 3-fold difference to control HCAEC. Regulated genes are involved in apoptosis, hemostasis, oxidative stress response, infection, and inflammation. Regulation of selected genes and proteins identified in the gene array analysis was confirmed by Real-time RT-PCR assay (granulocy te chemotactic protein 2), ELISA (urokinase, cyclooxygenase 2, granulocyte chemotactic protein 1), and western blotting (Heme oxygenase1, BCL2 interacting protein) at various time points between 4 and 24 hours. These results indicate that GBS infection might influence signalling pathways leading to impaired function of the innate immune system and hemorrhagic and inflammatory complications during GBS sepsis.