Refine
Has Fulltext
- yes (33)
Is part of the Bibliography
- yes (33)
Year of publication
Document Type
- Journal article (33)
Language
- English (33)
Keywords
- in vitro (3)
- tissue engineering (3)
- Neisseria gonorrhoeae (2)
- bioengineering (2)
- collagens (2)
- extracellular matrix (2)
- gene expression (2)
- inflammation (2)
- stem cells (2)
- 1st-line treatment (1)
- 3D in vitro models (1)
- 3D tissue model (1)
- 3D tissue models (1)
- 5-Fluorouracil (1)
- Alternative test methods (1)
- Articular-Cartilage (1)
- Assay (1)
- BRAF mutation (1)
- Beta-catenin (1)
- BioVaSc (1)
- Biomechanical Properties (1)
- Biomedical engineering (1)
- CYP induction (1)
- CYP inhibition (1)
- Carcinoma cells (1)
- Cardiac ventricles (1)
- Chemicals (1)
- Collagen (1)
- Compressive Properties (1)
- Data acquisition (1)
- Diffusion tensor imaging (1)
- Draize eye test (1)
- Eigenvectors (1)
- Episkin (1)
- Fibroblasts (1)
- Fluorouracil (1)
- Heart (1)
- Human Knee (1)
- Human Medial Meniscus (1)
- In vitro skin irritation testing (1)
- Injuries (1)
- Invasion (1)
- Magnetic resonance imaging (1)
- Matrix (1)
- Model (1)
- Models (1)
- Multicenter randomized-trial (1)
- OECD guideline (1)
- Open source reconstructed epidermis (1)
- Osteoarthritis (1)
- RHE (1)
- Repair (1)
- Salmonella (1)
- Stem-cell biotechnology (1)
- Swine (1)
- Tissue (1)
- Tractography (1)
- Triple co-culture (1)
- Validation (1)
- Vivo (1)
- adenocarcinoma of the lung (1)
- adhesion (1)
- adults (1)
- air-liquid interface (1)
- albumins (1)
- algorithm (1)
- alternative to animal testing (1)
- alternatives (1)
- anatomy (1)
- antennas (1)
- artificial membrane-permeability (1)
- asthmatic bronchial epithelium (1)
- astrocytes (1)
- autologous chondrocyte implantation (1)
- bioinformatics (1)
- biological models (1)
- biomaterial tests (1)
- biomedical engineering (1)
- biomimetic 3D tissue model (1)
- bioreactor (1)
- bioreactor culture (1)
- biotechnology (1)
- blood-brain barrier (BBB) model (1)
- calcium fluoride nanoparticles (1)
- cancer treatment (1)
- cell culture (1)
- cell engineering (1)
- cellular biology (1)
- cellular microenvironment (1)
- central nervous system (1)
- co-culture (1)
- colony-stimulating factor (1)
- colorectal cancer (1)
- combination of physical vapor deposition and electrochemical etching (1)
- combinatorial drug predictions (1)
- core depression (1)
- corneal equivalent (1)
- culture techniques (1)
- cultured (1)
- cytokines (1)
- cytotoxicity (1)
- decellularization (1)
- defined humanized test system (1)
- drug metabolism (1)
- dynamic culture conditions (1)
- early diagnosis (1)
- embryonic stem cells (1)
- enzyme metabolism (1)
- epidermis (1)
- epithelial cell culture (1)
- epithelial cells (1)
- equipment and supplies (1)
- establishment (1)
- experimental models of disease (1)
- expression (1)
- eye irritation testing (1)
- factor-VIII (1)
- fibroblast chemotaxis (1)
- foreign body reaction (1)
- gastrointestinal tract (1)
- gels (1)
- gene therapy (1)
- heptaocytes (1)
- host (1)
- human (1)
- human heptocytes (1)
- human induced pluripotent stem cells (hiPSCs)human induced pluripotent stem cells (hiPSCs) (1)
- immunotherapeutics (1)
- immunotherapies (1)
- in silico simulation (1)
- in situ guided tissue regeneration (1)
- in vitro models (1)
- in vivo (1)
- induction (1)
- infection (1)
- infectious disease (1)
- inflammation-induced tissue demage (1)
- inflammatory bowel disease (1)
- inflammatory response (1)
- invasion (1)
- invasiveness (1)
- label-free analysis (1)
- living cells (1)
- lncRNAs (1)
- lung and intrathoracic tumors (1)
- lung cancer (1)
- magnetic resonance imaging (MRI) (1)
- mechanisms of disease (1)
- medicine (1)
- mesenchymal cells (1)
- mesenchymal stem cells (1)
- mesenchymal tissues (1)
- miRNAs (1)
- microenvironment (1)
- microwave radiation (1)
- modular tumor tissue models (1)
- molecular biology (1)
- multifunctional nanoparticles (1)
- multimodal imaging (1)
- multipotent fetal neural stem cells (fNSCs) (1)
- murine (1)
- nanotopographical surfaces (1)
- neurovascular unit in vitro (1)
- neutrophil transmigration (1)
- non-invasive biomarkers (1)
- on-a-chip (1)
- oragnoids (1)
- osteochondral allografts (1)
- osteogenesis imperfecta (1)
- outgrowth endothelial cells (1)
- parasitology (1)
- perfusion-based bioreactor system (1)
- permeability (1)
- phenotype (1)
- photoluminescence (1)
- physiology (1)
- primary cell isolation (1)
- primary cells (1)
- progenitor cells (1)
- pulmonary drug-delivery (1)
- pulmonary imaging (1)
- quanititative characterization (1)
- quantification (1)
- raman spectroscopy (1)
- real time PCR (1)
- reconstructed human epidermis (1)
- reflection (1)
- regenerative medicine (1)
- respiratory syncytial virus (1)
- responses (1)
- scaffolds (1)
- secondary lung tumors (1)
- segmental collapse (1)
- sheep model (1)
- skin anatomy (1)
- skin equivalents (1)
- skin physiology (1)
- small intestinal submucosa scaffold (1)
- stratification (1)
- stromal cells (1)
- systemic inflammatory response syndrome (1)
- targeted therapy (1)
- tissue remodeling (1)
- trachea (1)
- transcriptomics (1)
- translational research (1)
- translocation (1)
- transport studies (1)
- tumor cells (1)
- tumor test system (1)
- upcyte hepatocytes (1)
- vascularization (1)
- vesicle-based barrier (1)
- virulence (1)
- xenotransplantation (1)
Institute
- Lehrstuhl für Tissue Engineering und Regenerative Medizin (33) (remove)
Sonstige beteiligte Institutionen
Cancer is one of the leading causes of death worldwide. Current therapeutic strategies are predominantly developed in 2D culture systems, which inadequately reflect physiological conditions in vivo. Biological 3D matrices provide cells an environment in which cells can self-organize, allowing the study of tissue organization and cell differentiation. Such scaffolds can be seeded with a mixture of different cell types to study direct 3D cell-cell-interactions. To mimic the 3D complexity of cancer tumors, our group has developed a 3D in vitro tumor test system.
Our 3D tissue test system models the in vivo situation of malignant peripheral nerve sheath tumors (MPNSTs), which we established with our decellularized porcine jejunal segment derived biological vascularized scaffold (BioVaSc). In our model, we reseeded a modified BioVaSc matrix with primary fibroblasts, microvascular endothelial cells (mvECs) and the S462 tumor cell line For static culture, the vascular structure of the BioVaSc is removed and the remaining scaffold is cut open on one side (Small Intestinal Submucosa SIS-Muc). The resulting matrix is then fixed between two metal rings (cell crowns).
Another option is to culture the cell-seeded SIS-Muc in a flow bioreactor system that exposes the cells to shear stress. Here, the bioreactor is connected to a peristaltic pump in a self-constructed incubator. A computer regulates the arterial oxygen and nutrient supply via parameters such as blood pressure, temperature, and flow rate. This setup allows for a dynamic culture with either pressure-regulated pulsatile or constant flow.
In this study, we could successfully establish both a static and dynamic 3D culture system for MPNSTs. The ability to model cancer tumors in a more natural 3D environment will enable the discovery, testing, and validation of future pharmaceuticals in a human-like model.
In situ guided tissue regeneration, also addressed as in situ tissue engineering or endogenous regeneration, has a great potential for population-wide “minimal invasive” applications. During the last two decades, tissue engineering has been developed with remarkable in vitro and preclinical success but still the number of applications in clinical routine is extremely small. Moreover, the vision of population-wide applications of ex vivo tissue engineered constructs based on cells, growth and differentiation factors and scaffolds, must probably be deemed unrealistic for economic and regulation-related issues. Hence, the progress made in this respect will be mostly applicable to a fraction of post-traumatic or post-surgery situations such as big tissue defects due to tumor manifestation. Minimally invasive procedures would probably qualify for a broader application and ideally would only require off the shelf standardized products without cells. Such products should mimic the microenvironment of regenerating tissues and make use of the endogenous tissue regeneration capacities. Functionally, the chemotaxis of regenerative cells, their amplification as a transient amplifying pool and their concerted differentiation and remodeling should be addressed. This is especially important because the main target populations for such applications are the elderly and diseased. The quality of regenerative cells is impaired in such organisms and high levels of inhibitors also interfere with regeneration and healing. In metabolic bone diseases like osteoporosis, it is already known that antagonists for inhibitors such as activin and sclerostin enhance bone formation. Implementing such strategies into applications for in situ guided tissue regeneration should greatly enhance the efficacy of tailored procedures in the future.
Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
(2012)
Non-destructive, non-contact and label-free technologies to monitor cell and tissue cultures are needed in the field of biomedical research.1-5 However, currently available routine methods require processing steps and alter sample integrity. Raman spectroscopy is a fast method that enables the measurement of biological samples without the need for further processing steps. This laser-based technology detects the inelastic scattering of monochromatic light.6 As every chemical vibration is assigned to a specific Raman band (wavenumber in cm-1), each biological sample features a typical spectral pattern due to their inherent biochemical composition.7-9 Within Raman spectra, the peak intensities correlate with the amount of the present molecular bonds.1 Similarities and differences of the spectral data sets can be detected by employing a multivariate analysis (e.g. principal component analysis (PCA)).10
Here, we perform Raman spectroscopy of living cells and native tissues. Cells are either seeded on glass bottom dishes or kept in suspension under normal cell culture conditions (37 °C, 5% CO2) before measurement. Native tissues are dissected and stored in phosphate buffered saline (PBS) at 4 °C prior measurements. Depending on our experimental set up, we then either focused on the cell nucleus or extracellular matrix (ECM) proteins such as elastin and collagen. For all studies, a minimum of 30 cells or 30 random points of interest within the ECM are measured. Data processing steps included background subtraction and normalization.