Refine
Has Fulltext
- yes (21)
Is part of the Bibliography
- yes (21)
Year of publication
Document Type
- Journal article (20)
- Doctoral Thesis (1)
Keywords
- melanoma (8)
- Merkel cell carcinoma (7)
- polyomavirus (4)
- large T antigen (2)
- peptide vaccination (2)
- therapy (2)
- A375 (1)
- CD274 (1)
- CD30 (1)
- CRISPR (1)
- Cutaneous lymphoma (1)
- DNA repair (1)
- Epigenetic regulation (1)
- FoxO3 (1)
- GSK3 (1)
- HDAC (1)
- Hautkrebs (1)
- Hippo signaling (1)
- Histone deacetylase (1)
- Immunoconjugate (1)
- JAK2 (1)
- Krebs <Medizin> (1)
- LSD1 (1)
- Large T antigen (1)
- Lysine-specific methylase (1)
- MCC (1)
- MHC class I chain-related protein (1)
- Melanom (1)
- Melanoma (1)
- Merkel cell polyomavirus (1)
- PD-L1 (1)
- PIK3CA mutations (1)
- Priming (1)
- STAT3 (1)
- T antigen (1)
- T-Lymphozyt (1)
- T-Zelle (1)
- T-antigen (1)
- T-cell (1)
- T-cell reactivity (1)
- TP53 (1)
- TRRAP (1)
- Tubulin (1)
- XPA (1)
- akt (1)
- antagomiRs (1)
- antibody–drug conjugates (1)
- apoptosis (1)
- artesunate (1)
- cancer (1)
- cancer treatment (1)
- cell cycle (1)
- cell staining (1)
- cultured fibroplasts (1)
- cutaneous T-cell lymphoma and Merkel cell carcinoma (1)
- cutaneous T-cell-lymphoma (1)
- epigenetic silencing (1)
- expression (1)
- ferroptosis (1)
- focal adhesion (1)
- glycogen synthase kinase 3 (1)
- heat shock response (1)
- immune suppression (1)
- immunoconjugate (1)
- interferon (1)
- knockout (1)
- large cell transformation (1)
- membrans proteins (1)
- miR-375 (1)
- mycosis fungoides (1)
- nucleotide excision repair (1)
- oncodermatology (1)
- p53 (1)
- panel sequencing (1)
- phthalazinone pyrazole (1)
- polymavirus (1)
- polymerase chain reaction (1)
- predictive marker (1)
- priming (1)
- protein variant (1)
- rare (1)
- regulatory T cell (1)
- retinoblastoma protein (1)
- single nucleotide polymorphism (1)
- skin cancer (1)
- skin squamous cell carcinoma (1)
- squamous cell (1)
- survivin (1)
- survivin T-cell reactivity (1)
- tertiary lymphoid tissue (1)
- tertiäres lymphatisches Gewebe (1)
- transcription factors (1)
- tumor-draining lymph node (1)
- tumors (1)
- viral carcinogenesis (1)
Institute
- Klinik und Poliklinik für Dermatologie, Venerologie und Allergologie (21)
- Theodor-Boveri-Institut für Biowissenschaften (2)
- Comprehensive Cancer Center Mainfranken (1)
- Institut für Klinische Transfusionsmedizin und Hämotherapie (1)
- Medizinische Klinik und Poliklinik II (1)
- Pathologisches Institut (1)
- Rudolf-Virchow-Zentrum (1)
EU-Project number / Contract (GA) number
- 277775 (2)
Merkel cell carcinoma (MCC) is an aggressive skin cancer frequently caused by the Merkel cell polyomavirus (MCPyV), and proliferation of MCPyV-positive MCC tumor cells depends on the expression of a virus-encoded truncated Large T antigen (LT) oncoprotein. Here, we asked in which phases of the cell cycle LT activity is required for MCC cell proliferation. Hence, we generated fusion-proteins of MCPyV-LT and parts of geminin (GMMN) or chromatin licensing and DNA replication factor1 (CDT1). This allowed us to ectopically express an LT, which is degraded either in the G1 or G2 phase of the cell cycle, respectively, in MCC cells with inducible T antigen knockdown. We demonstrate that LT expressed only in G1 is capable of rescuing LT knockdown-induced growth suppression while LT expressed in S and G2/M phases fails to support proliferation of MCC cells. These results suggest that the crucial function of LT, which has been demonstrated to be inactivation of the cellular Retinoblastoma protein 1 (RB1) is only required to initiate S phase entry.