Refine
Has Fulltext
- yes (74)
Is part of the Bibliography
- yes (74)
Year of publication
- 2019 (74) (remove)
Document Type
- Journal article (74) (remove)
Language
- English (74)
Keywords
- apoptosis (4)
- infection (3)
- leukemic cells (3)
- 3D tissue model (2)
- Bees (2)
- HeLa cells (2)
- Neural circuits (2)
- autophagy (2)
- cytotoxicity (2)
- genome annotation (2)
- metagenomics (2)
- p53 (2)
- sphingolipids (2)
- (classical and atypical) Werner syndrome (1)
- ABP1 (1)
- AUX1 (1)
- Accurate (1)
- Acids (1)
- Alpine habitats (1)
- Annotation (1)
- Anthropocene (1)
- Apidae (1)
- Apis mellifera (1)
- Aspergillus fumigalus (1)
- B-MYB (1)
- Barrier (1)
- Behavioural ecology (1)
- Berberine (1)
- C-60 fullerene (1)
- C60 fullerene (1)
- CD274 (1)
- CIDP (1)
- C\(_{60}\) fullerene (1)
- Cancer (1)
- Cancer Cell (1)
- Cell stainin (1)
- Cellular neuroscience (1)
- Central nervous system (1)
- Chemical composition (1)
- Chlamydia trachomatis (1)
- Circular dichroism (1)
- Colonization (1)
- Computational and Systems Biology (1)
- Computer modelling (1)
- Confocal microscopy (1)
- Conifers (1)
- Conservation (1)
- CpG (1)
- Cushing’s disease (1)
- Cytosol (1)
- DLS and AFM measurements (1)
- DNA damage (1)
- Dopamine (1)
- Doxorubicin (1)
- Dynamics (1)
- ERK signaling (1)
- Ecology (1)
- Embryonic induction (1)
- Environmental impact (1)
- Evolutionary developmental biology (1)
- Evolutionary emergence (1)
- Extracellular matrix (1)
- Fgf-signalling (1)
- Flowering plants (1)
- Flowers (1)
- Fluorescence spectroscopy (1)
- Forests (1)
- GABA (1)
- GABAA receptors (1)
- GAD1 (1)
- Gene expression analysis (1)
- Genome (1)
- Genomics (1)
- Germline (1)
- Health (1)
- Honey bees (1)
- Host-parasite interaction (1)
- Hypothalamus (1)
- Immunoprecipitation (1)
- Insect flight (1)
- JAK2 (1)
- Laparoscopy (1)
- Learning and memory (1)
- Limb development (1)
- Llullaillaco Volcano (1)
- Lymph nodes (1)
- M14 carboxypeptidasses (1)
- Maculinea butterfly (1)
- Mc4r (1)
- Melanoma (1)
- Methylation (1)
- Microbiology and Infectious Disease (1)
- Minimally invasive surgery (1)
- Mitochondria (1)
- Myb-MuvB (1)
- Myrmica ant non-equilibrium dynamics (1)
- Neisseria gonorrhoeae (1)
- Neisseria meningitidis (1)
- Oncology (1)
- Outer membrane proteins (1)
- PD-L1 (1)
- PTEN (1)
- Phenols (1)
- Plants (1)
- Poplars (1)
- Protein folding (1)
- Protein kinase D3 (PKD3) (1)
- Proteus vulgaris (1)
- R package (1)
- RNA metabolism (1)
- RNA sequencing (1)
- RNA-seq transcriptome (1)
- RNAi (1)
- Research Article (1)
- Scarabaeidae (1)
- Self-renewal (1)
- Serotonin (1)
- Sex chromosome (1)
- Sex determination (1)
- Sexual development and function (1)
- Small interfering RNAs (1)
- Solid tumors (1)
- Solution-state NMR (1)
- Species delimitation (1)
- Strains (1)
- Subtercola vilae (1)
- Surgical and invasive medical procedures (1)
- Surgical oncology (1)
- TNNI3 (1)
- TP53 (1)
- Transcription (1)
- Transcriptomic (1)
- Translation (1)
- Transposable element (1)
- Trees (1)
- UV–Vis (1)
- Ustilago maydis (1)
- V-ATPase (1)
- YAP (1)
- abdominal surgery (1)
- accumulation (1)
- acetate (1)
- alternative splicing (1)
- altitudinal gradients (1)
- animal physiology (1)
- anti-contactin-1 (1)
- autoantibody (1)
- auxin (1)
- behavioral plasticity (1)
- bioinformatics tool (1)
- biomarker signature (1)
- biotic interaction (1)
- bisulfite pyrosequencing (1)
- bladder (1)
- boolean modeling (1)
- brain (1)
- brain development (1)
- brain disorders (1)
- burnt-wood (1)
- cancer (1)
- cancer metabolism (1)
- cancer therapy (1)
- carabid beetles (1)
- cardiolipin (1)
- cardiomyopathy (1)
- catheterization (1)
- catheters (1)
- cell biology (1)
- cell migration (1)
- ceramide (1)
- chlamydia (1)
- cholesterol (1)
- circRNA (1)
- circular transcriptome sequencing (1)
- cisplatin (1)
- co-culture (1)
- cold adaptation (1)
- collybistin (1)
- colorectal cancer (1)
- comparative genomics (1)
- competition (1)
- complement deposition (1)
- conservation (1)
- dead-wood enrichment (1)
- dendritic cell (1)
- dendritic cells (1)
- deubiquitinases (1)
- developmental forms (1)
- diacylglycerol (DAG) (1)
- dimeric peptide (1)
- direct muss spectrometric profiling (1)
- diversity gradients (1)
- domain-specific language (1)
- doxorubicin (1)
- drug release (1)
- ecology (1)
- ecosystem service (1)
- efficient intervention points (1)
- elementary body (1)
- endocytosis (1)
- enercy-richness hypothesis (1)
- energy homeostasis (1)
- enhancer (1)
- epidural block (1)
- eugenol (1)
- evolutionary genetics (1)
- expansion microscopy (1)
- extinction dynamics (1)
- fertility (1)
- fission (1)
- fluorescent probes (1)
- food resources (1)
- foraging patterns (1)
- forest fire (1)
- forest management (1)
- friut fly behaviour (1)
- functional analysis (1)
- fungal rhodopsins (1)
- gangliosides and lipid rafts (1)
- gastrointestinal tract (1)
- gene expression analysis (1)
- genetic engineering (1)
- genetics (1)
- genome analysis (1)
- genome assembly (1)
- genomics (1)
- gephyrin (1)
- glioblastoma multiforme (1)
- global change (1)
- glucose transporter (1)
- ground-dwelling predators (1)
- growth (1)
- histone H2AX (1)
- honeybee (1)
- honeybees (1)
- human xenografted mouse models (1)
- ichthyology (1)
- imaging (1)
- indole-3-acetic acid (1)
- infection biology (1)
- insect abundance (1)
- insect collection (1)
- integrative management strategy (1)
- intracellular bacterial pathogens (1)
- irradiation (1)
- knockout (1)
- land sharing (1)
- land use (1)
- lipid metabolism (1)
- liver (1)
- lowland beech forests (1)
- lysosome (1)
- mRNA (1)
- mTOR (1)
- machine learning (1)
- mating preference (1)
- measles virus (1)
- mechanisms of disease (1)
- mechanistic modelling (1)
- medaka (1)
- meta-analysis (1)
- metabolic adaptation (1)
- metabolic flux (1)
- metabolic modeling (1)
- metabolic modelling (1)
- metabolism (1)
- metabolism of infected and uninfected host cells (1)
- methods (1)
- methylation array (1)
- microbial rhodopsins (1)
- microbiome (1)
- migration (1)
- mitochondria (1)
- mitochondrial genome (1)
- mitotic genes (1)
- molecular biology (1)
- mutants (1)
- nanocomplex (1)
- native populations (1)
- natural pest control (1)
- nervous system (1)
- nest microbiota (1)
- neuronal (1)
- next-generation sequencing (1)
- noncovalent complex (1)
- noncovalent nanocomplex (1)
- nuclear envelope (1)
- nuclear export (1)
- oncogenes (1)
- oncology (1)
- oncolytic virus (1)
- optimal drug combination (1)
- optimal drug targeting (1)
- optimal pharmacological modulation (1)
- optimal treatment strategies (1)
- paranodopathy (1)
- parasite biology (1)
- passive transfer (1)
- patch-clamp (1)
- pathogenesis (1)
- pathogenic bacteria (1)
- pediatrics (1)
- peptide inhibitor design (1)
- peptidomoics (1)
- photodynamic chemotherapy (1)
- piRNA (1)
- plant–microbe–pollinator triangle (1)
- plasma membrane depolarization (1)
- pollination (1)
- pollination network (1)
- population genetics (1)
- post-fire management (1)
- premature aging (1)
- protected forests (1)
- protein processing (1)
- protein-protein interaction (PPI) (1)
- psychiatric disorders (1)
- puberty (1)
- radiation (1)
- radiation sensitivity (1)
- rectum (1)
- reprogamming of host cell metabolism (1)
- resonance theory (1)
- reticulate body (1)
- rheumatoid arthritis (1)
- risk factors (1)
- saproxylic organisms (1)
- sarcomere (1)
- secreted effectors (1)
- segmental progeria (1)
- sentinel prey (1)
- signalling (1)
- single-molecule tracking (1)
- small intestinal submucosa scaffold (1)
- small-cell lung cancer (1)
- soil fauna (1)
- solitary bees (1)
- somatic mutations (1)
- species richness (1)
- species‐area hypothesis (1)
- sphingosine-1-phosphate (1)
- sporidia (1)
- structured illumination microscopy (1)
- super resolution microscopy (1)
- super-resolution microscopy (1)
- superresolution (1)
- surgical and invasive medical procedures (1)
- survival analysis (1)
- synergistic effect (1)
- systematic affiliation (1)
- targeted therapies (1)
- temperature‐mediated resource exploitation hypothesis (1)
- temperature‐richness hypothesis (1)
- time lag (1)
- topminnow (1)
- transcription (1)
- transcription deficiency (1)
- transcriptomics (1)
- transient dynamics (1)
- transposable elements (1)
- tree cavities (1)
- trypanosomes (1)
- unmanaged broadleaved forests (1)
- uptake (1)
- urinary tract infections (1)
- viruses (1)
- whole-genome sequencing (1)
- wild bees (1)
- wound healing (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (74) (remove)
Fanconi anemia (FA) is a genetically heterogeneous disorder with 22 disease-causing genes reported to date. In some FA genes, monoallelic mutations have been found to be associated with breast cancer risk, while the risk associations of others remain unknown. The gene for FA type C, FANCC, has been proposed as a breast cancer susceptibility gene based on epidemiological and sequencing studies. We used the Oncoarray project to genotype two truncating FANCC variants (p.R185X and p.R548X) in 64,760 breast cancer cases and 49,793 controls of European descent. FANCC mutations were observed in 25 cases (14 with p.R185X, 11 with p.R548X) and 26 controls (18 with p.R185X, 8 with p.R548X). There was no evidence of an association with the risk of breast cancer, neither overall (odds ratio 0.77, 95%CI 0.44–1.33, p = 0.4) nor by histology, hormone receptor status, age or family history. We conclude that the breast cancer risk association of these two FANCC variants, if any, is much smaller than for BRCA1, BRCA2 or PALB2 mutations. If this applies to all truncating variants in FANCC it would suggest there are differences between FA genes in their roles on breast cancer risk and demonstrates the merit of large consortia for clarifying risk associations of rare variants.
Animals must slow or halt locomotion to integrate sensory inputs or to change direction. In Caenorhabditis elegans, the GABAergic and peptidergic neuron RIS mediates developmentally timed quiescence. Here, we show RIS functions additionally as a locomotion stop neuron. RIS optogenetic stimulation caused acute and persistent inhibition of locomotion and pharyngeal pumping, phenotypes requiring FLP-11 neuropeptides and GABA. RIS photoactivation allows the animal to maintain its body posture by sustaining muscle tone, yet inactivating motor neuron oscillatory activity. During locomotion, RIS axonal Ca2+ signals revealed functional compartmentalization: Activity in the nerve ring process correlated with locomotion stop, while activity in a branch correlated with induced reversals. GABA was required to induce, and FLP-11 neuropeptides were required to sustain locomotion stop. RIS attenuates neuronal activity and inhibits movement, possibly enabling sensory integration and decision making, and exemplifies dual use of one cell across development in a compact nervous system.
In most organisms, ribosomal RNA (rRNA) contributes to >85% of total RNA. Thus, to obtain useful information from RNA-sequencing (RNA-seq) analyses at reasonable sequencing depth, typically, mature polyadenylated transcripts are enriched or rRNA molecules are depleted. Targeted depletion of rRNA is particularly useful when studying transcripts lacking a poly(A) tail, such as some non-coding RNAs (ncRNAs), most bacterial RNAs and partially degraded or immature transcripts. While several commercially available kits allow effective rRNA depletion, their efficiency relies on a high degree of sequence homology between oligonucleotide probes and the target RNA. This restricts the use of such kits to a limited number of organisms with conserved rRNA sequences. In this study we describe the use of biotinylated oligos and streptavidin-coated paramagnetic beads for the efficient and specific depletion of trypanosomal rRNA. Our approach reduces the levels of the most abundant rRNA transcripts to less than 5% with minimal off-target effects. By adjusting the sequence of the oligonucleotide probes, our approach can be used to deplete rRNAs or other abundant transcripts independent of species. Thus, our protocol provides a useful alternative for rRNA removal where enrichment of polyadenylated transcripts is not an option and commercial kits for rRNA are not available.
Icefishes (suborder Notothenioidei; family Channichthyidae) are the only vertebrates that lack functional haemoglobin genes and red blood cells. Here, we report a high-quality genome assembly and linkage map for the Antarctic blackfin icefish Chaenocephalus aceratus, highlighting evolved genomic features for its unique physiology. Phylogenomic analysis revealed that Antarctic fish of the teleost suborder Notothenioidei, including icefishes, diverged from the stickleback lineage about 77 million years ago and subsequently evolved cold-adapted phenotypes as the Southern Ocean cooled to sub-zero temperatures. Our results show that genes involved in protection from ice damage, including genes encoding antifreeze glycoprotein and zona pellucida proteins, are highly expanded in the icefish genome. Furthermore, genes that encode enzymes that help to control cellular redox state, including members of the sod3 and nqo1 gene families, are expanded, probably as evolutionary adaptations to the relatively high concentration of oxygen dissolved in cold Antarctic waters. In contrast, some crucial regulators of circadian homeostasis (cry and per genes) are absent from the icefish genome, suggesting compromised control of biological rhythms in the polar light environment. The availability of the icefish genome sequence will accelerate our understanding of adaptation to extreme Antarctic environments.