Filtern
Volltext vorhanden
- ja (24)
Gehört zur Bibliographie
- ja (24)
Dokumenttyp
Sprache
- Englisch (24)
Schlagworte
- positron emission tomography (8)
- PET (7)
- Positronen-Emissions-Tomografie (6)
- 18F-LMI1195 (3)
- norepinephrine transporter (3)
- sympathetic nervous system (3)
- 11C-HED (2)
- SPECT (2)
- ageing (2)
- diabetes (2)
- heart failure (2)
- molecular medicine (2)
- precision medicine (2)
- 11C-Hydroxyephedrine (1)
- 11C-hydroxyephedrine (1)
- 123I-metaiodobenzylguanidine (1)
- 18F-FDS (1)
- 18F-flurpiridaz (1)
- 18FFBnTP (1)
- 99mTc-DTPA (1)
- Alpha (1)
- Alpha power (1)
- Alzheimer’s disease (1)
- Body movement (1)
- Cardiovascular diseases (1)
- Cognition (1)
- Cognitive processing (1)
- ECG (1)
- FV45 (1)
- Glomerular filtration (1)
- HFmrEF (1)
- HMDP hydroxymethylene diphosphonate (1)
- Heart failure (1)
- Herz (1)
- Kognition (1)
- MPI (1)
- MRI (1)
- Mobie EEG (1)
- Myocardial-perfusion SPECT (1)
- Natural walking (1)
- Nierenfunktionsstörung (1)
- Positronenemissionstomografie (1)
- SPECT Scanner (1)
- Sodium-Glucose Cotransporters (SGLTs) (1)
- T-shaped π-π stacking (1)
- T-shaped π–π stacking (1)
- Walking (1)
- ZDF rats (1)
- \(^{18}\)F-FDG (1)
- \(^{18}\)F-fluorodeoxyglucose (1)
- alpha oscillations (1)
- amyloid-β (Aβ) (1)
- angiotensin II type 1 receptor (1)
- antidepressant (1)
- autonomic nervous system (1)
- benzylguanidine (1)
- biomarkers (1)
- blinks (1)
- butyrylcholinesterase (1)
- carbamate (1)
- cardiac (1)
- cardiac innervation imaging (1)
- cardiac neurohormonal system (1)
- cardiac sympathetic nervous system (1)
- coronary artery disease (1)
- diabetic cardiomyopathy (1)
- ejection fraction (1)
- endocrinology (1)
- enzyme kinetics (1)
- fluorine-18 (1)
- heart (1)
- heart failure with mid-range ejection fraction (1)
- hydroxyephedrine (1)
- left-ventricular function (1)
- locomotion (1)
- medium-sized animals (1)
- mobile EEG (1)
- motor entrainment (1)
- moycardial sympathetic innervation (1)
- myocardial perfusion imaging (1)
- myocardial sympathetic innervation imaging (1)
- nonhuman primates (1)
- nuclear cardiology (1)
- organic cation transporter (1)
- performance (1)
- personalized treatment (1)
- phaeochromocytoma (1)
- phenethylguanidine (1)
- pupil size (1)
- quality (1)
- radiotracer (1)
- radiotracer kinetics (1)
- radiotracers (1)
- rats (1)
- renal failure (1)
- renin-angiotensin system (1)
- saccades (1)
- scanner (1)
- single photon emission computed tomography: sympathetic nerve (1)
- skeletal (1)
- small animal SPECT (1)
- stem cells (1)
- stem-cell research (1)
- storage vesicle turnover (1)
- structure–activity relationships (1)
- sympathetic nerve (1)
- unilateral ureteral obstruction (1)
- valsartan (1)
- walking phase (1)
Institut
- Klinik und Poliklinik für Nuklearmedizin (22)
- Institut für Pharmazie und Lebensmittelchemie (6)
- Deutsches Zentrum für Herzinsuffizienz (DZHI) (5)
- Institut für Psychologie (2)
- Medizinische Klinik und Poliklinik I (2)
- Graduate School of Life Sciences (1)
- Institut für diagnostische und interventionelle Radiologie (Institut für Röntgendiagnostik) (1)
Sonstige beteiligte Institutionen
- Johns Hopkins School of Medicine (4)
- Johns Hopkins School of Medicine, Baltimore, MD, USA (1)
- Johns Hopkins University School of Medicine (1)
- Johns Hopkins University School of Medicine, Baltimore, MD, U.S. (1)
- Johns Hopkins University, Baltimore, MD, U.S. (1)
- National Cardiovascular and Cerebral Center, Suita, Japan (1)
Objectives. This study is aimed at investigating the impact of frame numbers in preclinical electrocardiogram- (ECG-) gated \(^{18}\)F-fluorodeoxyglucose (\(^{18}\)F-FDG) positron emission tomography (PET) on systolic and diastolic left ventricular (LV) parameters in rats. Methods. \(^{18}\)F-FDG PET imaging using a dedicated small animal PET system with list mode data acquisition and continuous ECG recording was performed in diabetic and control rats. The list-mode data was sorted and reconstructed with different numbers of frames (4, 8, 12, and 16) per cardiac cycle into tomographic images. Using an automatic ventricular edge detection software, left ventricular (LV) functional parameters, including ejection fraction (EF), end-diastolic (EDV), and end-systolic volume (ESV), were calculated. Diastolic variables (time to peak filling (TPF), first third mean filling rate (1/3 FR), and peak filling rate (PFR)) were also assessed. Results. Significant differences in multiple parameters were observed among the reconstructions with different frames per cardiac cycle. EDV significantly increased by numbers of frames (353.8 & PLUSMN; 57.7 mu l*, 380.8 & PLUSMN; 57.2 mu l*, 398.0 & PLUSMN; 63.1 mu l*, and 444.8 & PLUSMN; 75.3 mu l at 4, 8, 12, and 16 frames, respectively; *P < 0.0001 vs. 16 frames), while systolic (EF) and diastolic (TPF, 1/3 FR and PFR) parameters were not significantly different between 12 and 16 frames. In addition, significant differences between diabetic and control animals in 1/3 FR and PFR in 16 frames per cardiac cycle were observed (P < 0.005), but not for 4, 8, and 12 frames. Conclusions. Using ECG-gated PET in rats, measurements of cardiac function are significantly affected by the frames per cardiac cycle. Therefore, if you are going to compare those functional parameters, a consistent number of frames should be used.
We aimed to determine a detailed regional ventricular distribution pattern of the novel cardiac nerve PET radiotracer \(^{18}\)F-LMI1195 in healthy rabbits. Ex-vivo high resolution autoradiographic imaging was conducted to identify accurate ventricular distribution of \(^{18}\)F-LMI1195. In healthy rabbits, \(^{18}\)F-LMI1195 was administered followed by the reference perfusion marker \(^{201}\)Tl for a dual-radiotracer analysis. After 20 min of \(^{18}\)F-LMI1195 distribution time, the rabbits were euthanized, the hearts were extracted, frozen, and cut into 20-μm short axis slices. Subsequently, the short axis sections were exposed to a phosphor imaging plate to determine \(^{18}\)F-LMI1195 distribution (exposure for 3 h). After complete \(^{18}\)F decay, sections were re-exposed to determine 201Tl distribution (exposure for 7 days). For quantitative analysis, segmental regions of Interest (ROIs) were divided into four left ventricular (LV) and a right ventricular (RV) segment on mid-ventricular short axis sections. Subendocardial, mid-portion, and subepicardial ROIs were placed on the LV lateral wall. \(^{18}\)F-LMI1195 distribution was almost homogeneous throughout the LV wall without any significant differences in all four LV ROIs (anterior, posterior, septal and lateral wall, 99 ± 2, 94 ± 5, 94 ± 4 and 97 ± 3%LV, respectively, n.s.). Subepicardial \(^{201}\)Tl uptake was significantly lower compared to the subendocardial portion (subendocardial, mid-portion, and subepicardial activity: 90 ± 3, 96 ± 2 and *80 ± 5%LV, respectively, *p < 0.01 vs. mid-portion). This was in contradistinction to the transmural wall profile of \(^{18}\)F-LMI1195 (90 ± 4, 96 ± 5 and 84 ± 4%LV, n.s.). A slight but significant discrepant transmural radiotracer distribution pattern of \(^{201}\)Tl in comparison to \(^{18}\)F-LMI1195 may be a reflection of physiological sympathetic innervation and perfusion in rabbit hearts.
Brown adipose tissue (BAT) is an attractive therapeutic target to combat diabetes and obesity due to its ability to increase glucose expenditure. In a genetic rat model (ZDF fa/fa) of type-2 diabetes and obesity, we aimed to investigate glucose utilization of BAT by \(^{18}\)F-FDG PET imaging. Male Zucker diabetic fatty (ZDF) and Male Zucker lean (ZL) control rats were studied at 13 weeks. Three weeks prior to imaging, ZDF rats were randomized into a no-restriction (ZDF-ND) and a mild calorie restriction (ZDF-CR) group. Dynamic \(^{18}\)F-FDG PET using a dedicated small animal PET system was performed under hyperinsulinemic-euglycemic clamp. \(^{18}\)F-FDG PET identified intense inter-scapular BAT glucose uptake in all ZL control rats, while no focally increased \(^{18}\)F-FDG uptake was detected in all ZDF-ND rats. Mild but significant improved BAT tracer uptake was identified after calorie restriction in diabetic rats (ZDF-CR). The weight of BAT tissue and fat deposits were significantly increased in ZDF-CR and ZDF-ND rats as compared to ZL controls, while UCP-1 and mitochondrial concentrations were significantly decreased. Whitening and severely impaired insulin-stimulated glucose uptake in BAT was confirmed in a rat model of type-2 diabetes. Additionally, calorie restriction partially restored the impaired BAT glucose uptake.