Refine
Has Fulltext
- yes (82)
Is part of the Bibliography
- yes (82)
Year of publication
- 2017 (82) (remove)
Document Type
- Journal article (65)
- Doctoral Thesis (17)
Keywords
- biology (8)
- Apis mellifera (4)
- Trypanosoma (4)
- ants (4)
- social systems (4)
- Drosophila (3)
- Drosophila melanogaster (3)
- animal sociality (3)
- fungi (3)
- symbiosis (3)
- Biology (2)
- Chlamydia trachomatis (2)
- DNA damage (2)
- DNA methylation (2)
- Latrophilin (2)
- Neisseria (2)
- Trypanosoma brucei (2)
- carbon dioxide (2)
- cell differentiation (2)
- ceramide (2)
- chemotherapy (2)
- comparative genomics (2)
- cytokines (2)
- fibroblasts (2)
- foraging (2)
- genetic variation (2)
- humidity (2)
- learning (2)
- lung cancer (2)
- memory (2)
- metabolism (2)
- methylation (2)
- mushroom bodies (2)
- nesting habits (2)
- pollen (2)
- toxins (2)
- 3D Ko-kulture (1)
- 3D cell culture (1)
- 3D-Zellkulturen (Krebstherapie) (1)
- 5-fluorouracil (1)
- Acyrthosiphon pisum (1)
- Adhesion-GPCR (1)
- Agrobacterium vitis (1)
- Angewandte Mikrobiologie (1)
- Angiotensin II (1)
- Angiotensin II Typ 1a-Rezeptor (1)
- Antimikrobieller Wirkstoff (1)
- Apoptosis (1)
- Aspergillus fumigatus (1)
- B cell receptors (1)
- B cells (1)
- BMP (1)
- BRAF inhibition (1)
- Bauchspeicheldrüsenkrebs (1)
- Bruchpilot (1)
- Burkina Faso (1)
- CHO cell culture (1)
- CHO-Zelle (1)
- Caenorhabditis elegans (1)
- Cell surface proteomics (1)
- Chlamydia (1)
- Circadian rhythms and sleep (1)
- CoA (1)
- Complexin (1)
- Computer software (1)
- DNA Barcoding (1)
- DNS-Schädigung (1)
- DREAM complex (1)
- Detektion (1)
- Diagnostik (1)
- Drugtargets (1)
- Dunce isoforms (1)
- EZH2 (1)
- Enzyme Regulation (1)
- Enzyme kinetics (1)
- Enzyme metabolism (1)
- Enzyme regulation (1)
- Enzymes (1)
- Epicardium-derived cells (1)
- Epigenetics (1)
- Exposure Risk (1)
- Fibroblasten (1)
- GPCR (1)
- Genetics (1)
- Genexpression <Molekulargenetik> (1)
- Geschlechtsbestimmung (1)
- Geschlechtsdifferenzierung (1)
- HECT Ligase (1)
- HUWE1 (1)
- Holobiont (1)
- Human atrial stromal cells (1)
- Immune Escape (1)
- Interaktionen (1)
- Japankärpfling (1)
- K-Ras (1)
- Kryptolebias marmoratus (1)
- Landschaftsstruktur (1)
- Lungenkrebs (1)
- MAP-Kinase (1)
- MICA (1)
- MICB (1)
- MITE (1)
- MMB (1)
- Mcl-1 (1)
- Medaka (1)
- Medicine (1)
- Megalobrama amblycephala (1)
- Megaponera analis (1)
- Meiose (1)
- Meiosis (1)
- Melanom (1)
- Meliponini (1)
- Merkel cell carcinoma (1)
- Merkel-Zellkarzinom (1)
- Metabolic pathways (1)
- Mikrobiota (1)
- Mitose (1)
- Molecular Biophysics (1)
- Molekulare Hybridisierung (1)
- Monozytendifferenzierung (1)
- N-terminal domain (1)
- NAD (1)
- NADPH oxidase (1)
- NADPH-Oxidase (1)
- NFATc1 (1)
- Neisseria gonorrhoeae (1)
- Neisseria meningitidis (1)
- Neuromuscular junctions (1)
- Next-Generation Sequenzierung (1)
- Nicht-kleinzelliges Bronchialkarzinom (NSCLC) (1)
- Nährboden (1)
- OGEE v2 (1)
- Oilseed Rape (1)
- Oxidativer Stress (1)
- P14ARF (1)
- PDE4d (1)
- PER (1)
- Pipecolinsäurederivate (1)
- Pollen (1)
- Predictive toxicology (1)
- Produktivität (1)
- RNA in situ hybridization (1)
- RNA interference (1)
- Resource Use (1)
- Retinoesäure (1)
- Retinoic acid (1)
- Saccharomyces cerevisiae (1)
- Sammeldistanzen (1)
- Senescence (1)
- Septins (1)
- Sex determination (1)
- Staphylococcus aureus (1)
- Stoffwechsel (1)
- Structural Biology (1)
- Synapse (1)
- Synapses (1)
- Synaptic vesicles (1)
- Systembiologie (1)
- Systembiologische Analysen (1)
- T cells (1)
- T-cadherin (1)
- TAMs (1)
- TP53 (1)
- Telomer (1)
- Toxicity (1)
- Transcriptional control (1)
- Tumor Immunology (1)
- Ubiquitin (1)
- Vaccinia virus (1)
- Vesicles (1)
- Weinrebe (1)
- Wilms tumour (1)
- Wurzelhalsgalle (1)
- X-Ray Chrystallography (1)
- Zea mays (1)
- Zebrafish (1)
- Zellkultur (1)
- Zytokinine (Pflanzenpathogene) (1)
- active zone (1)
- acute myeloid leukaemia (1)
- adhesion GPCR (1)
- agroecology (1)
- alcohol tolerance (1)
- alkaloids (1)
- alu elements (1)
- anaplasia (1)
- angiotensin II type 1a receptor (1)
- antibiotics (1)
- antimicrobials (1)
- bakterielle Flora (1)
- bees (1)
- behavior (1)
- behavioural ecology (1)
- biogenic amines (1)
- bioinformatics (1)
- biomarker (1)
- biomaterials (1)
- biophysics (1)
- blood (1)
- blood platelets (1)
- bone (1)
- brain (1)
- brain development (1)
- breast cancer (1)
- cadherin-13 (CDH13) (1)
- cancer (1)
- cancer treatment (1)
- cash crops (1)
- catabolism (1)
- cell binding (1)
- cell cycle (1)
- cell cycle and cell division (1)
- cell proliferation (1)
- cell-autonomous defense (1)
- cellular function (1)
- cellular stress (1)
- central clocks (1)
- central complex (1)
- ceramide analogs (1)
- cholera (1)
- chromatin remodeling (1)
- chronobiology (1)
- circadian clock (1)
- circadian mechanisms (1)
- clinical genetics (1)
- cloning (1)
- cognition (1)
- colorectal carcinoma (1)
- compound conditioning (1)
- confocal laser scanning microscopy (1)
- confocal-microscopy based automated quantification (1)
- cotton (1)
- crotonase (1)
- cryptic (1)
- cytokinesis (1)
- cytotoxic T cells (1)
- dCIRL (1)
- damped circadian clock (1)
- decision-making (1)
- deformed wing virus (1)
- dehydrogenase (1)
- desert ants (1)
- deubiquitinase (1)
- developmental differentiation (1)
- differential olfactory conditioning (1)
- division of labor (1)
- dorsal raphe (1)
- dunce (1)
- eEF1A1 (1)
- eclosion (1)
- ecological genetics (1)
- ecosystem services (1)
- endophyte (1)
- epigenetics (1)
- eukaryota (1)
- evolutionary genetics (1)
- evolutionary response (1)
- fetal cord blood (1)
- fetal programming (1)
- floral resource distribution (1)
- fluorescence imaging (1)
- forager (1)
- foraging behaviour (1)
- foraging distances (1)
- functional complementarity (1)
- functional redundancy (1)
- fungal endophytes of grasses (1)
- fungal infection (1)
- fungal physiology (1)
- fungal structure (1)
- gambiense (1)
- gene essentiality database (1)
- gene expression (1)
- gene regulation (1)
- genetic loci (1)
- genome collection (1)
- genome integrity (1)
- genome sequencing (1)
- genomic libraries (1)
- genomic sequence (1)
- genomics research (1)
- gestational diabetes mellitus (1)
- glioblastoma multiforme (1)
- glycoprotein Ib (1)
- glycosylation (1)
- gonococcal (1)
- gonococcal infection (1)
- grass (1)
- gut microflora (1)
- habitat information (1)
- halbnatürliche Habitate (1)
- hangover (1)
- herbivorous diet (1)
- herpes virus (1)
- high throughput (1)
- high-throughput screening (1)
- histones (1)
- honey bees (1)
- honeybees (1)
- human cancer cell lines (1)
- humanized mice (1)
- hyperexpression techniques (1)
- ichthyology (1)
- image analysis (1)
- imaging the immune system (1)
- immune cells (1)
- immune evasion (1)
- infectious diseases (1)
- infectious-disease diagnostics (1)
- infrared radiation (1)
- insect (1)
- insulin treatment (1)
- intermuscular bone (1)
- kinesin (1)
- landscape ecology (1)
- landscape structure (1)
- leaves (1)
- light pulses (1)
- lipids (1)
- look-back behavior (1)
- lymphocyte activation (1)
- macrophages (1)
- mass spectrometry (1)
- mating success (1)
- mechanobiology (1)
- mechanosensing (1)
- mechanotransduction (1)
- media design (1)
- megakaryocytes (1)
- membrane receptor signaling (1)
- messenger RNA (1)
- metabotropic signalling (1)
- metagenomics (1)
- miRNA-Detektion (1)
- miRNS (1)
- microRNAs (1)
- microRNA–target interaction (1)
- microbiome (1)
- microswimmer (1)
- migration (1)
- mitochondria (1)
- mitotic gene expression (1)
- molecular diagnostics (1)
- molecular neuroscience (1)
- molting (1)
- monocyte (1)
- monocyte differentiation (1)
- morphometry (1)
- multiple myeloma (1)
- mushroom body (1)
- natural killer cells (1)
- neisseria meningitidis (1)
- nephroblastoma (1)
- nesting habit (1)
- networks (1)
- neuroanatomy (1)
- neurodevelopment (1)
- neuronal development (1)
- neuropeptide pathway (1)
- next-generation sequencing (1)
- none (1)
- nurse bee (1)
- nutrition (1)
- nutritional ecology (1)
- octopamine (1)
- optogenetics (1)
- orientation (1)
- osteocytes (1)
- oxaliplatin (1)
- oxidative stress (1)
- pancreatic cancer (1)
- parasitic cell cycles (1)
- parasitic diseases (1)
- pattern recognition receptors (1)
- perception (1)
- peripheral clocks (1)
- plant defense (1)
- plant-insect interactions (1)
- plant–insect interactions (1)
- point-of-care (1)
- pollination (1)
- pollinator decline (1)
- population structure (1)
- prefrontal cortex (1)
- proGenomes (1)
- proboscis extension reflex (1)
- product qualitymodulation (1)
- prokaryotic clade (1)
- prokaryotic subspecies (1)
- protein structure (1)
- proteins (1)
- proteomes (1)
- psychiatric disorders (1)
- quality control (1)
- radial glia (1)
- reactivating p53 and inducing tumor apoptosis (RITA) (1)
- recruitment (1)
- regulation (1)
- regulatory T cells (1)
- regulatory circuit downstream (1)
- relA (1)
- replication (1)
- reproductive asynchrony (1)
- rescue behavior (1)
- resin (1)
- retinal development (1)
- sacbrood virus (1)
- season (1)
- semi-natural habitat (1)
- sensory physiology (1)
- sept3 (1)
- sept5a (1)
- sept5b (1)
- septin (1)
- sequence assembly tools (1)
- serotonin (1)
- sesame (1)
- sex-specific mortality (1)
- sexual dimorphism in timing (1)
- signal transduction (1)
- signaling (1)
- sky-compass pathway (1)
- sleeping sickness (1)
- small interfering RNAs (1)
- social immunity (1)
- sphingolipids (1)
- sphingomyelinase (1)
- spider (1)
- spidroin (1)
- string database (1)
- stringent response (1)
- structural biology (1)
- substrate channeling (1)
- super-resolution microscopy (1)
- synaptic vesicle tethering (1)
- systems biology (1)
- taxonomic description (1)
- telomere-binding protein (1)
- tetracyclines (1)
- therapeutic strategy (1)
- throat (1)
- transcriptome (1)
- treatment (1)
- tsetse fly (1)
- tumour heterogeneity (1)
- vaccinia virus (1)
- vector navigation (1)
- video recording (1)
- virulenceregulatory evolution (1)
- visual orientation (1)
- whole genome (1)
- yellow fluorescent protein (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (82) (remove)
Sonstige beteiligte Institutionen
Staphylococcus aureus (S. aureus) infections are a major clinical problem and range from mild skin and soft-tissue infections to severe and even lethal infections such as pneumonia, endocarditis, sepsis, osteomyelitis, and toxic shock syndrome. Toxins that are released from S. aureus mediate many of these effects. Here, we aimed to identify molecular mechanisms how α-toxin, a major S. aureus toxin, induces inflammation. Methods: Macrophages were isolated from the bone marrow of wildtype and acid sphingomyelinase-deficient mice, stimulated with S. aureus α-toxin and activation of the acid sphingomyelinase was quantified. The subcellular formation of ceramides was determined by confocal microscopy. Release of cathepsins from lysosomes, activation of inflammasome proteins and formation of Interleukin-1β (IL-1β) and Tumor Necrosis Factor-α (TNF-α) were analyzed by western blotting, confocal microscopy and ELISA. Results: We demonstrate that S. aureus α-toxin activates the acid sphingomyelinase in ex vivo macrophages and triggers a release of ceramides. Ceramides induced by S. aureus α-toxin localize to lysosomes and mediate a release of cathepsin B and D from lysosomes into the cytoplasm. Cytosolic cathepsin B forms a complex with Nlrc4. Treatment of macrophages with α-toxin induces the formation of IL-1β and TNF-α. These events are reduced or abrogated, respectively, in cells lacking the acid sphingomyelinase and upon treatment of macrophages with amitriptyline, a functional inhibitor of acid sphingomyelinase. Pharmacological inhibition of cathepsin B prevented activation of the inflammasome measured as release of IL-1β, while the formation of TNF-α was independent of cathepsin B. Conclusion: We demonstrate a novel mechanism how bacterial toxins activate the inflammasome and mediate the formation and release of cytokines: S. aureus α-toxin triggers an activation of the acid sphingomyelinase and a release of ceramides resulting in the release of lysosomal cathepsin B and formation of pro-inflammatory cytokines.
A system-wide understanding of cellular function requires knowledge of all functional interactions between the expressed proteins. The STRING database aims to collect and integrate this information, by consolidating known and predicted protein–protein association data for a large number of organisms. The associations in STRING include direct (physical) interactions, as well as indirect (functional) interactions, as long as both are specific and biologically meaningful. Apart from collecting and reassessing available experimental data on protein–protein interactions, and importing known pathways and protein complexes from curated databases, interaction predictions are derived from the following sources: (i) systematic co-expression analysis, (ii) detection of shared selective signals across genomes, (iii) automated text-mining of the scientific literature and (iv) computational transfer of interaction knowledge between organisms based on gene orthology. In the latest version 10.5 of STRING, the biggest changes are concerned with data dissemination: the web frontend has been completely redesigned to reduce dependency on outdated browser technologies, and the database can now also be queried from inside the popular Cytoscape software framework. Further improvements include automated background analysis of user inputs for functional enrichments, and streamlined download options. The STRING resource is available online, at http://string-db.org/.
The Kryptolebias marmoratus is unique because it is the only selffertilizing hermaphroditic vertebrate, known to date. It primarily reproduces by internal self-fertilization in a mixed ovary/testis gonad. Here, we report on a high-quality genome assembly for the K. marmoratus South Korea (SK) strain highlighting the diversity and distribution of transposable elements (TEs). We find that K. marmoratus genome maintains number and composition of TEs. This can be an important genomic attribute promoting genome recombination in this selfing fish, while, in addition to a mixed mating strategy, it may also represent a mechanism contributing to the evolutionary adaptation to ecological pressure of the species. Future work should help clarify this point further once genomic information is gathered for other taxa of the family Rivulidae that do not self-fertilize. We provide a valuable genome resource that highlights the potential impact of TEs on the genome evolution of a fish species with an uncommon life cycle.
OGEE is an Online GEne Essentiality database. To enhance our understanding of the essentiality of genes, in OGEE we collected experimentally tested essential and non-essential genes, as well as associated gene properties known to contribute to gene essentiality. We focus on large-scale experiments, and complement our data with text-mining results. We organized tested genes into data sets according to their sources, and tagged those with variable essentiality statuses across data sets as conditionally essential genes, intending to highlight the complex interplay between gene functions and environments/experimental perturbations. Developments since the last public release include increased number of species and gene essentiality data sets, inclusion of non-coding essential sequences and genes with intermediate essentiality statuses. In addition, we included 16 essentiality data sets from cancer cell lines, corresponding to 9 human cancers; with OGEE, users can easily explore the shared and differentially essential genes within and between cancer types. These genes, especially those derived from cell lines that are similar to tumor samples, could reveal the oncogenic drivers, paralogous gene expression pattern and chromosomal structure of the corresponding cancer types, and can be further screened to identify targets for cancer therapy and/or new drug development. OGEE is freely available at http://ogee.medgenius.info.
Alterations to the gene encoding the EZH2 (KMT6A) methyltransferase, including both gain-of-function and loss-of-function, have been linked to a variety of haematological malignancies and solid tumours, suggesting a complex, context-dependent role of this methyltransferase. The successful implementation of molecularly targeted therapies against EZH2 requires a greater understanding of the potential mechanisms by which EZH2 contributes to cancer. One aspect of this effort is the mapping of EZH2 partner proteins and cellular targets. To this end we performed affinity-purification mass spectrometry in the FAB-M2 HL-60 acute myeloid leukaemia (AML) cell line before and after all-trans retinoic acid-induced differentiation. These studies identified new EZH2 interaction partners and potential non-histone substrates for EZH2-mediated methylation. Our results suggest that EZH2 is involved in the regulation of translation through interactions with a number of RNA binding proteins and by methylating key components of protein synthesis such as eEF1A1. Given that deregulated mRNA translation is a frequent feature of cancer and that eEF1A1 is highly expressed in many human tumours, these findings present new possibilities for the therapeutic targeting of EZH2 in AML.
Automatic image reconstruction is critical to cope with steadily increasing data from advanced microscopy. We describe here the Fiji macro 3D ART VeSElecT which we developed to study synaptic vesicles in electron tomograms. We apply this tool to quantify vesicle properties (i) in embryonic Danio rerio 4 and 8 days past fertilization (dpf) and (ii) to compare Caenorhabditis elegans N2 neuromuscular junctions (NMJ) wild-type and its septin mutant (unc-59(e261)). We demonstrate development-specific and mutant-specific changes in synaptic vesicle pools in both models. We confirm the functionality of our macro by applying our 3D ART VeSElecT on zebrafish NMJ showing smaller vesicles in 8 dpf embryos then 4 dpf, which was validated by manual reconstruction of the vesicle pool. Furthermore, we analyze the impact of C. elegans septin mutant unc-59(e261) on vesicle pool formation and vesicle size. Automated vesicle registration and characterization was implemented in Fiji as two macros (registration and measurement). This flexible arrangement allows in particular reducing false positives by an optional manual revision step. Preprocessing and contrast enhancement work on image-stacks of 1nm/pixel in x and y direction. Semi-automated cell selection was integrated. 3D ART VeSElecT removes interfering components, detects vesicles by 3D segmentation and calculates vesicle volume and diameter (spherical approximation, inner/outer diameter). Results are collected in color using the RoiManager plugin including the possibility of manual removal of non-matching confounder vesicles. Detailed evaluation considered performance (detected vesicles) and specificity (true vesicles) as well as precision and recall. We furthermore show gain in segmentation and morphological filtering compared to learning based methods and a large time gain compared to manual segmentation. 3D ART VeSElecT shows small error rates and its speed gain can be up to 68 times faster in comparison to manual annotation. Both automatic and semi-automatic modes are explained including a tutorial.
\(Ambra1\) is linked to autophagy and neurodevelopment. Heterozygous \(Ambra1\) deficiency induces autism-like behavior in a sexually dimorphic manner. Extraordinarily, autistic features are seen in female mice only, combined with stronger Ambra1 protein reduction in brain compared to males. However, significance of \(AMBRA1\) for autistic phenotypes in humans and, apart from behavior, for other autism-typical features, namely early brain enlargement or increased seizure propensity, has remained unexplored. Here we show in two independent human samples that a single normal \(AMBRA1\) genotype, the intronic SNP rs3802890-AA, is associated with autistic features in women, who also display lower \(AMBRA1\) mRNA expression in peripheral blood mononuclear cells relative to female GG carriers. Located within a non-coding RNA, likely relevant for mRNA and protein interaction, rs3802890 (A versus G allele) may affect its stability through modification of folding, as predicted by \(in\) \(silico\) analysis. Searching for further autism-relevant characteristics in \(Ambra1^{+/−}\) mice, we observe reduced interest of female but not male mutants regarding pheromone signals of the respective other gender in the social intellicage set-up. Moreover, altered pentylentetrazol-induced seizure propensity, an \(in\) \(vivo\) readout of neuronal excitation–inhibition dysbalance, becomes obvious exclusively in female mutants. Magnetic resonance imaging reveals mild prepubertal brain enlargement in both genders, uncoupling enhanced brain dimensions from the primarily female expression of all other autistic phenotypes investigated here. These data support a role of \(AMBRA1/Ambra1\) partial loss-of-function genotypes for female autistic traits. Moreover, they suggest \(Ambra1\) heterozygous mice as a novel multifaceted and construct-valid genetic mouse model for female autism.
Intracellular pathogenic microorganisms and toxins exploit host cell mechanisms to enter, exert their deleterious effects as well as hijack host nutrition for their development. A potential approach to treat multiple pathogen infections and that should not induce drug resistance is the use of small molecules that target host components. We identifed the compound 1-adamantyl (5-bromo-2-methoxybenzyl) amine (ABMA) from a cell-based high throughput screening for its capacity to protect human cells and mice against ricin toxin without toxicity. This compound efciently protects cells against various toxins and pathogens including viruses, intracellular bacteria and parasite. ABMA provokes Rab7-positive late endosomal compartment accumulation in mammalian cells without affecting other organelles (early endosomes, lysosomes, the Golgi apparatus, the endoplasmic reticulum or the nucleus). As the mechanism of action of ABMA is restricted to host-endosomal compartments, it reduces cell infection by pathogens that depend on this pathway to invade cells. ABMA may represent a novel class of broad-spectrum compounds with therapeutic potential against diverse severe infectious diseases.
Quantifying protein densities on cell membranes using super-resolution optical fluctuation imaging
(2017)
Quantitative approaches for characterizing molecular organization of cell membrane molecules under physiological and pathological conditions profit from recently developed super-resolution imaging techniques. Current tools employ statistical algorithms to determine clusters of molecules based on single-molecule localization microscopy (SMLM) data. These approaches are limited by the ability of SMLM techniques to identify and localize molecules in densely populated areas and experimental conditions of sample preparation and image acquisition. We have developed a robust, model-free, quantitative clustering analysis to determine the distribution of membrane molecules that excels in densely labeled areas and is tolerant to various experimental conditions, i.e. multiple-blinking or high blinking rates. The method is based on a TIRF microscope followed by a super-resolution optical fluctuation imaging (SOFI) analysis. The effectiveness and robustness of the method is validated using simulated and experimental data investigating nanoscale distribution of CD4 glycoprotein mutants in the plasma membrane of T cells.
Multifunctional enzyme, type-1 (MFE1) is a monomeric enzyme with a 2E-enoyl-CoA hydratase and a 3S-hydroxyacyl-CoA dehydrogenase (HAD) active site. Enzyme kinetic data of rat peroxisomal MFE1 show that the catalytic efficiencies for converting the short-chain substrate 2E-butenoyl-CoA into acetoacetyl-CoA are much lower when compared with those of the homologous monofunctional enzymes. The mode of binding of acetoacetyl-CoA (to the hydratase active site) and the very similar mode of binding of NAD\(^+\) and NADH (to the HAD part) are described and compared with those of their monofunctional counterparts. Structural comparisons suggest that the conformational flexibility of the HAD and hydratase parts of MFE1 are correlated. The possible importance of the conformational flexibility of MFE1 for its biocatalytic properties is discussed.