Refine
Has Fulltext
- yes (80)
Is part of the Bibliography
- yes (80)
Year of publication
- 2023 (80) (remove)
Document Type
- Journal article (56)
- Doctoral Thesis (21)
- Preprint (3)
Language
- English (80) (remove)
Keywords
- Biene (5)
- animal behaviour (4)
- altitudinal gradient (3)
- bee (3)
- global warming (3)
- toe (3)
- winter wheat (3)
- Alps (2)
- Apis mellifera (2)
- CRISPR/Cas9 (2)
- Mikroskopie (2)
- Monarchfalter (2)
- Orientierung (2)
- Zellzyklus (2)
- abandonment (2)
- cancer microenvironment (2)
- central complex (2)
- cosmology (2)
- crystallization (2)
- decoherence (2)
- division of labor (2)
- emergent time (2)
- global change (2)
- grading (2)
- honeybee (2)
- immunotherapy (2)
- infection (2)
- machine learning (2)
- qubit (2)
- sustainable agriculture (2)
- transcription (2)
- translational research (2)
- 18S rRNA (1)
- 3D tissue model (1)
- 3D-Zellkultur (1)
- A. thaliana (1)
- ADSCs (1)
- ATF4 (1)
- Agentenbasierte Modellierung (1)
- AmGr1 (1)
- AmGr1, AmGr2, AmGr3 (1)
- AmGr2 (1)
- AmGr3 (1)
- Antikörper (1)
- B-MYB (1)
- Baumhöhle (1)
- Bestäubung (1)
- Biene <Gattung> (1)
- Bienenkrankheiten (1)
- Bienenschwarm (1)
- Bildverarbeitung (1)
- Bimolekulare Lipidschicht (1)
- Bioinformatics (1)
- Biologie (1)
- Bispecific T-cell engager (1)
- Brassicaceae (1)
- Brownsche Bewegung (1)
- CD117 (1)
- CHAC1 (1)
- CNV (1)
- Cancer (1)
- Candida albicans (1)
- Cell cycle (1)
- Chromatin (1)
- Colorectal Cancer (1)
- Compressed Sensing (1)
- Coxiella burnetii (1)
- DNA (1)
- DNA methylation (1)
- DNA storage (1)
- DNS-Reparatur (1)
- DNS-Schädigung (1)
- Danaus plexippus (1)
- Datenanalyse (1)
- Dickdarmkrebs (1)
- Differentialgleichung (1)
- EEG (1)
- Elektrophysiologie (1)
- Embryonalentwicklung (1)
- Endocytose (1)
- Endocytosis (1)
- Endosomes (1)
- European orchard bee (Osmia cornuta) (1)
- Fabaceae (1)
- Flight muscle (1)
- Freies Molekül (1)
- Fructosebisphosphat-Aldolase (1)
- Förster Resonance Energy Transfer (1)
- G2/M genes (1)
- GIS (1)
- GPVI (1)
- GSH (1)
- Gehirn (1)
- Genexpression (1)
- Genomics (1)
- Genotyping (1)
- Genregulation (1)
- Hemibodies (1)
- Hemibody (1)
- Honeybee (1)
- IL-4 antagonists (1)
- Imkerei (1)
- Immunreaktion (1)
- Insekten (1)
- Insektensterben (1)
- Interleukin-4 (IL-4) (1)
- KIT (1)
- Kakao (1)
- Ki67 (1)
- Kolloid (1)
- Kolorektales Karzinom (1)
- Krebsforschung (1)
- Künstliche Intelligenz (1)
- Landsat 8 (1)
- Landschaftspflege (1)
- LeishBASEedit (1)
- Leishmania (1)
- Lepidoptera (1)
- Lifetime Imaging (1)
- Lysosome (1)
- MMB (1)
- MOD13Q1 (1)
- MODIS (1)
- MYC (1)
- MYCNv (1)
- Mamestra brassicae (1)
- Mathematische Modellierung (1)
- Mc4r (1)
- Monitoring (1)
- Monoklonaler bispezifischer Antikörper (1)
- Multi-Unit Aufnahmen (1)
- NDVI (1)
- NHX1 (1)
- NOTCH (1)
- NRF2 (1)
- NaCl transport (1)
- Narrow escape problem (1)
- Neisseria gonorrhoeae (1)
- Nisthöhle (1)
- OLFM4 (1)
- Octopamin (1)
- Octopamine (1)
- Octopaminergic signaling (1)
- Olfaktorik (1)
- Oncogenes (1)
- Organoid (1)
- PCR (1)
- PER (1)
- Phylogenie (1)
- Phylogeny (1)
- RCC (1)
- RNA (1)
- RNA transport (1)
- RNA-binding proteins (1)
- Random-walk simulations (1)
- Rbm8a (1)
- Ribosom (1)
- SARS-CoV-2 (1)
- SLC7A11 (1)
- Salmonella Typhimurium (1)
- Salt Overly Sensitive pathway (1)
- Schmetterlinge (1)
- Schädlingsbekämpfung (1)
- Sehen (1)
- Sentinel-2 (1)
- Sequence-Structure (1)
- Spechte (1)
- T cells (1)
- T-Lymphozyt (1)
- T-cell engager (1)
- Thermogenesis (1)
- Tissue Engineering (1)
- Transkription <Genetik> (1)
- Transkriptionelle Regulierung (1)
- Transkriptomanalyse (1)
- Trypanosoma (1)
- Trypanosoma brucei (1)
- Trypanosomiasis (1)
- Vaccinia (1)
- Vaccinia-virus (1)
- Vicia faba (L.) (1)
- Visuelle Orientierung (1)
- Visuelle Wahrnehmung (1)
- Volatile Organic Compound (VOC) (1)
- Wald (1)
- Web services (1)
- Wilde Honigbienen (1)
- Xiphophorus (1)
- YAP (1)
- Zellmigration (1)
- alternating management (1)
- amino acid transporter (1)
- animal physiology (1)
- annotation (1)
- anti-thrombotic therapies (1)
- artificial human skin (1)
- atopic diseases (1)
- azido-ceramides (1)
- bacterial migration (1)
- bacterial virulence (1)
- bee diseases (1)
- bee-lining (1)
- bees (1)
- biodiversity conservation (1)
- biological scaffold (1)
- biology (1)
- biomarker (1)
- biosynthetic glycosylation (1)
- bipartite metabolism (1)
- bit (1)
- bitter taste (1)
- blood platelets (1)
- body size (1)
- brain (1)
- brain signal complexity (1)
- breast cancer (1)
- breed predisposition (1)
- c-kit (1)
- calcium signaling (1)
- cancer (1)
- canine (1)
- canola (1)
- carbon dioxide (CO2) (1)
- cell migration (1)
- cell velocimetry (1)
- cellular signalling networks (1)
- ceramides (1)
- chemical ecology (1)
- chemical glycosylation (1)
- chlorantraniliprole (1)
- chromatin accessibility (1)
- circadian rhythm (1)
- climate change (1)
- climate‐smart pest management (1)
- closing of chromatin (1)
- cognitive ability (1)
- collagen (1)
- color (1)
- computer modelling (1)
- conservation biology (1)
- control group (1)
- crop modeling (1)
- crop models (1)
- crop rotation (1)
- cuticular hydrocarbon (1)
- cytosine base editor (CBE) toolbox (1)
- cytoskeleton (1)
- cytosolic pH (1)
- cytotoxic T cells (1)
- dSTORM (1)
- data storage (1)
- ddPCR (1)
- decay (1)
- decision making (1)
- decision-making (1)
- desiccation (1)
- developmental differentiation (1)
- diet (1)
- digit (1)
- dog (1)
- drought stress (1)
- dual targeting (1)
- dust microbiomes (1)
- dynamic protein-protein interactions (1)
- echinocytes (1)
- ecology (1)
- ecosystem function (1)
- electrophysiology (1)
- electroporation (1)
- enhancers (1)
- entomology (1)
- epigenetic modification (1)
- error (1)
- error-transfer (1)
- euglena (1)
- euglenids (1)
- explainability of machine learning (1)
- feature analysis (1)
- feature selection (1)
- feeding (1)
- feral bees (1)
- filamentous Salmonella Typhimurium (1)
- flowering plants (1)
- flowers (1)
- fluorescence lifetime imaging microscopy (1)
- forager (1)
- forest landscape (1)
- fusion (1)
- gene (1)
- gene editing (1)
- genetic screen (1)
- genetics (1)
- genomics (1)
- glycoengineering (1)
- grasshopper (1)
- grazing (1)
- gustatory receptors (Grs) (1)
- haircoat (1)
- hemoglobin jet (1)
- hemolymph lipids (1)
- herpes virus (1)
- honey bee density (1)
- honeybee taste perception (1)
- hymenoptera (1)
- in silico analysis (1)
- individual drug responses (1)
- indoxacarb (1)
- insect conservation (1)
- insecticide (1)
- integrated stress response (1)
- intelligence (1)
- intestinal enteroids (1)
- juvenile hormone (1)
- kidney cancer (1)
- kinase signaling (1)
- land-use intensification (1)
- landsat (1)
- landscape complexity (1)
- landscape management (1)
- landwirtschaftlicher Betrieb (1)
- leaf response (1)
- light-driven metabolism (1)
- lipidomics (1)
- local farm management (1)
- locomotor activity (1)
- lymphocyte differentiation (1)
- malnourishment (1)
- mean first passage time (1)
- mechanisms of disease (1)
- melanoma (1)
- membrane biophysics (1)
- meta-data (1)
- metagenomics (1)
- miR (1)
- microbial diversity (1)
- microbial ecology (1)
- microstates (1)
- miniature schnauzer (1)
- model reduction (1)
- modified inflation (1)
- molecular neuroscience (1)
- monarch butterfly (1)
- monitoring (1)
- mountain (1)
- mountain biodiversity (1)
- mouse xenografts (1)
- multi-unit recording (1)
- multisensory navigation (1)
- mushroom body (1)
- myeloma (1)
- natural processing (1)
- navigation (1)
- necrobiome (1)
- network analysis (1)
- network simulation (1)
- neuroblastoma cell (1)
- neuronal and synaptic plasticity (1)
- neuroscience (1)
- non-SBI fungicide (1)
- non-muscle myosin (1)
- nurse bee (1)
- oil seed rape (1)
- oil-seed rape (1)
- oilseed rape (1)
- olfaction (1)
- olfactomedin 4 (1)
- oncolytic virus (1)
- opening of chromatin (1)
- optogenetics (1)
- orientation (1)
- osmotic effects (1)
- ovarian tumor (1)
- ozone (O3) (1)
- parasitoid (1)
- parasitology (1)
- patient data (1)
- perfusion culture (1)
- personalized medicine (1)
- pesticide mixture (1)
- phase space (1)
- phase transition (1)
- phosphoproteome (1)
- phylogenetics (1)
- plant physiology (1)
- plant-herbivore interactions (1)
- plant-insect interactions (1)
- plant–herbivore interactions (1)
- pollen beetle (1)
- pollinator (1)
- pollinators (1)
- pollution (1)
- pore (1)
- posttranscriptional regulation (1)
- potassium channel (1)
- precision agriculture (1)
- precision-cut tumor slices (1)
- preclinical model (1)
- principal (1)
- proboscis extension response (PER) (1)
- proteasome system (1)
- protein analysis (1)
- protein folding (1)
- proteomics (1)
- puberty (1)
- qubit interaction (1)
- quiescence (1)
- random forest (1)
- range shifts (1)
- reporter screen (1)
- resolution (1)
- responsiveness (1)
- resting-state (1)
- ribosome biogenesis (1)
- risk stratification (1)
- sRNA (1)
- salt stress (1)
- satellite (1)
- scalable functional genomic screening (1)
- secondary structure (1)
- seed yield (1)
- sentinel-2 (1)
- sequencing (1)
- signalling pathways (1)
- single cell analysis (1)
- single particle tracking (1)
- single-cell RNA sequencing (1)
- single-molecule biophysics (1)
- single-molecule localization microscopy (1)
- skin equivalent (1)
- sky kinases (1)
- species range shifts (1)
- sphingolipids (1)
- spider (1)
- standard schnauzer (1)
- stem cells (1)
- stem weevil (1)
- sublethal effect (1)
- sublethal effects (1)
- succession (1)
- sugar perception (fructose, sucrose) (1)
- sugar receptor (1)
- sugar responsiveness (1)
- swarming (1)
- synergistic effect (1)
- synthetic biology (1)
- thrombosis (1)
- tools overview (1)
- toxicity (1)
- traditional almond orchard (1)
- traditional land use (1)
- transcriptional regulation (1)
- transcriptome (1)
- transcriptomics (1)
- translocation experiment (1)
- tree cavity (1)
- trispecific (1)
- tumor microenvironment (1)
- tumour (1)
- tumour immunology (1)
- upslope shift (1)
- urbanization (1)
- vacuolar calcium sensor (1)
- venous infiltration (1)
- vineyard terrace (1)
- vision (1)
- visual cue (1)
- visual memory (1)
- visual perception (1)
- voltage gating (1)
- waggle dance decoding (1)
- wild honey bees (1)
- wild-living honey bees (1)
- wildlife-friendly farming (1)
- wrong labelling (1)
- zoology (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (80) (remove)
Sonstige beteiligte Institutionen
EU-Project number / Contract (GA) number
- 101041177 (1)
- 721016 (1)
- 741491 (1)
- 955910 (1)
- ESF-ZDEX 4.0 (1)
To fire action-potential-like electrical signals, the vacuole membrane requires the two-pore channel TPC1, formerly called SV channel. The TPC1/SV channel functions as a depolarization-stimulated, non-selective cation channel that is inhibited by luminal Ca\(^{2+}\). In our search for species-dependent functional TPC1 channel variants with different luminal Ca\(^{2+}\) sensitivity, we found in total three acidic residues present in Ca\(^{2+}\) sensor sites 2 and 3 of the Ca\(^{2+}\)-sensitive AtTPC1 channel from Arabidopsis thaliana that were neutral in its Vicia faba ortholog and also in those of many other Fabaceae. When expressed in the Arabidopsis AtTPC1-loss-of-function background, wild-type VfTPC1 was hypersensitive to vacuole depolarization and only weakly sensitive to blocking luminal Ca\(^{2+}\). When AtTPC1 was mutated for these VfTPC1-homologous polymorphic residues, two neutral substitutions in Ca\(^{2+}\) sensor site 3 alone were already sufficient for the Arabidopsis At-VfTPC1 channel mutant to gain VfTPC1-like voltage and luminal Ca\(^{2+}\) sensitivity that together rendered vacuoles hyperexcitable. Thus, natural TPC1 channel variants exist in plant families which may fine-tune vacuole excitability and adapt it to environmental settings of the particular ecological niche.
Transmission of Trypanosoma brucei by tsetse flies involves the deposition of the cell cycle-arrested metacyclic life cycle stage into mammalian skin at the site of the fly’s bite. We introduce an advanced human skin equivalent and use tsetse flies to naturally infect the skin with trypanosomes. We detail the chronological order of the parasites’ development in the skin by single-cell RNA sequencing and find a rapid activation of metacyclic trypanosomes and differentiation to proliferative parasites. Here we show that after the establishment of a proliferative population, the parasites enter a reversible quiescent state characterized by slow replication and a strongly reduced metabolism. We term these quiescent trypanosomes skin tissue forms, a parasite population that may play an important role in maintaining the infection over long time periods and in asymptomatic infected individuals.
Immune checkpoint blockade therapy is beneficial and even curative for some cancer patients. However, the majority don’t respond to immune therapy. Across different tumor types, pre-existing T cell infiltrates predict response to checkpoint-based immunotherapy. Based on in vitro pharmacological studies, mouse models and analyses of human melanoma patients, we show that the cytokine GDF-15 impairs LFA-1/β2-integrin-mediated adhesion of T cells to activated endothelial cells, which is a pre-requisite of T cell extravasation. In melanoma patients, GDF-15 serum levels strongly correlate with failure of PD-1-based immune checkpoint blockade therapy. Neutralization of GDF-15 improves both T cell trafficking and therapy efficiency in murine tumor models. Thus GDF-15, beside its known role in cancer-related anorexia and cachexia, emerges as a regulator of T cell extravasation into the tumor microenvironment, which provides an even stronger rationale for therapeutic anti-GDF-15 antibody development.
Salt stress is a major abiotic stress, responsible for declining agricultural productivity. Roots are regarded as hubs for salt detoxification, however, leaf salt concentrations may exceed those of roots. How mature leaves manage acute sodium chloride (NaCl) stress is mostly unknown.
To analyze the mechanisms for NaCl redistribution in leaves, salt was infiltrated into intact tobacco leaves. It initiated pronounced osmotically‐driven leaf movements. Leaf downward movement caused by hydro‐passive turgor loss reached a maximum within 2 h.
Salt‐driven cellular water release was accompanied by a transient change in membrane depolarization but not an increase in cytosolic calcium ion (Ca\(^{2+}\)) level. Nonetheless, only half an hour later, the leaves had completely regained turgor. This recovery phase was characterized by an increase in mesophyll cell plasma membrane hydrogen ion (H\(^{+}\)) pumping, a salt uptake‐dependent cytosolic alkalization, and a return of the apoplast osmolality to pre‐stress levels. Although, transcript numbers of abscisic acid‐ and Salt Overly Sensitive pathway elements remained unchanged, salt adaptation depended on the vacuolar H\(^{+}\)/Na\(^{+}\)‐exchanger NHX1.
Altogether, tobacco leaves can detoxify sodium ions (Na\(^{+}\)) rapidly even under massive salt loads, based on pre‐established posttranslational settings and NHX1 cation/H+ antiport activity. Unlike roots, signaling and processing of salt stress in tobacco leaves does not depend on Ca\(^{2+}\) signaling.
Efficient spatial orientation in the natural environment is crucial for the survival of most animal species. Cataglyphis desert ants possess excellent navigational skills. After far-ranging foraging excursions, the ants return to their inconspicuous nest entrance using celestial and panoramic cues. This review focuses on the question about how naïve ants acquire the necessary spatial information and adjust their visual compass systems. Naïve ants perform structured learning walks during their transition from the dark nest interior to foraging under bright sunlight. During initial learning walks, the ants perform rotational movements with nest-directed views using the earth’s magnetic field as an earthbound compass reference. Experimental manipulations demonstrate that specific sky compass cues trigger structural neuronal plasticity in visual circuits to integration centers in the central complex and mushroom bodies. During learning walks, rotation of the sky-polarization pattern is required for an increase in volume and synaptic complexes in both integration centers. In contrast, passive light exposure triggers light-spectrum (especially UV light) dependent changes in synaptic complexes upstream of the central complex. We discuss a multisensory circuit model in the ant brain for pathways mediating structural neuroplasticity at different levels following passive light exposure and multisensory experience during the performance of learning walks.
Highlights
• The integrated stress response leads to a general ATF4-dependent activation of NRF2
• ATF4 causes a CHAC1-dependent GSH depletion, resulting in NRF2 stabilization
• An elevation of NRF2 transcript levels fosters this effect
• NRF2 supports the ISR/ATF4 pathway by improving cystine and antioxidant supply
Summary
The redox regulator NRF2 becomes activated upon oxidative and electrophilic stress and orchestrates a response program associated with redox regulation, metabolism, tumor therapy resistance, and immune suppression. Here, we describe an unrecognized link between the integrated stress response (ISR) and NRF2 mediated by the ISR effector ATF4. The ISR is commonly activated after starvation or ER stress and plays a central role in tissue homeostasis and cancer plasticity. ATF4 increases NRF2 transcription and induces the glutathione-degrading enzyme CHAC1, which we now show to be critically important for maintaining NRF2 activation. In-depth analyses reveal that NRF2 supports ATF4-induced cells by increasing cystine uptake via the glutamate-cystine antiporter xCT. In addition, NRF2 upregulates genes mediating thioredoxin usage and regeneration, thus balancing the glutathione decrease. In conclusion, we demonstrate that the NRF2 response serves as second layer of the ISR, an observation highly relevant for the understanding of cellular resilience in health and disease.
Herpes simplex virus 1 (HSV-1) infection and stress responses disrupt transcription termination by RNA Polymerase II (Pol II). In HSV-1 infection, but not upon salt or heat stress, this is accompanied by a dramatic increase in chromatin accessibility downstream of genes. Here, we show that the HSV-1 immediate-early protein ICP22 is both necessary and sufficient to induce downstream open chromatin regions (dOCRs) when transcription termination is disrupted by the viral ICP27 protein. This is accompanied by a marked ICP22-dependent loss of histones downstream of affected genes consistent with impaired histone repositioning in the wake of Pol II. Efficient knock-down of the ICP22-interacting histone chaperone FACT is not sufficient to induce dOCRs in ΔICP22 infection but increases dOCR induction in wild-type HSV-1 infection. Interestingly, this is accompanied by a marked increase in chromatin accessibility within gene bodies. We propose a model in which allosteric changes in Pol II composition downstream of genes and ICP22-mediated interference with FACT activity explain the differential impairment of histone repositioning downstream of genes in the wake of Pol II in HSV-1 infection.
Most of the studies in cell biology primarily focus on models from the opisthokont group of eukaryotes. However, opisthokonts do not encompass the full diversity of eukaryotes. Thus, it is necessary to broaden the research focus to other organisms to gain a comprehensive understanding of basic cellular processes shared across the tree of life. In this sense, Trypanosoma brucei, a unicellular eukaryote, emerges as a viable alternative. The collaborative efforts in genome sequencing and protein tagging over the past two decades have significantly expanded our knowledge on this organism and have provided valuable tools to facilitate a more detailed analysis of this parasite. Nevertheless, numerous questions still remain.
The survival of T. brucei within the mammalian host is intricately linked to the endo-lysosomal system, which plays a critical role in surface glycoprotein recycling, antibody clearance, and plasma membrane homeostasis. However, the dynamics of the duplication of the endo-lysosomal system during T. brucei proliferation and its potential relationship with plasma membrane growth remain poorly understood. Thus, as the primary objective, this thesis explores the endo-lysosomal system of T. brucei in the context of the cell cycle, providing insights on cell surface growth, endosome duplication, and clathrin recruitment. In addition, the study revisits ferritin endocytosis to provide quantitative data on the involvement of TbRab proteins (TbRab5A, TbRab7, and TbRab11) and the different endosomal subpopulations (early, late, and recycling endosomes, respectively) in the transport of this fluid-phase marker. Notably, while these subpopulations function as distinct compartments, different TbRabs can be found within the same region or structure, suggesting a potential physical connection between the endosomal subpopulations. The potential physical connection of endosomes is further explored within the context of the cell cycle and, finally, the duplication and morphological plasticity of the lysosome are also investigated. Overall, these findings provide insights into the dynamics of plasma membrane growth and the coordinated duplication of the endo-lysosomal system during T. brucei proliferation. The early duplication of endosomes suggests their potential involvement in plasma membrane growth, while the late duplication of the lysosome indicates a reduced role in this process. The recruitment of clathrin and TbRab GTPases to the site of endosome formation supports the assumption that the newly formed endosomal system is active during cell division and, consequently, indicates its potential role in plasma membrane homeostasis.
Furthermore, considering the vast diversity within the Trypanosoma genus, which includes ~500 described species, the macroevolution of the group was investigated using the combined information of the 18S rRNA gene sequence and structure. The sequence-structure analysis of T. brucei and other 42 trypanosome species was conducted in the context of the diversity of Trypanosomatida, the order in which trypanosomes are placed. An additional analysis focused on Trypanosoma highlighted key aspects of the group’s macroevolution. To explore these aspects further, additional trypanosome species were included, and the changes in the Trypanosoma tree topology were analyzed. The sequence-structure phylogeny confirmed the independent evolutionary history of the human pathogens T. brucei and Trypanosoma cruzi, while also providing insights into the evolution of the Aquatic clade, paraphyly of groups, and species classification into subgenera.
Background: The cabbage moth, Mamestra brassicae, is a polyphagous pest that attacks several crops. Here, the sublethal and lethal effects of chlorantraniliprole and indoxacarb were investigated on the developmental stages, detoxification enzymes, reproductive activity, calling behavior, peripheral physiology, and pheromone titer of M. brasssicae. Methods: To assess pesticide effects, the second instar larvae were maintained for 24 h on a semi-artificial diet containing insecticides at their LC\(_{10}\), LC\(_{30}\), and LC\(_{50}\) concentrations. Results: M. brassicae was more susceptible to chlorantraniliprole (LC\(_{50}\) = 0.35 mg/L) than indoxacarb (LC\(_{50}\) = 1.71 mg/L). A significantly increased developmental time was observed with both insecticides at all tested concentrations but decreases in pupation rate, pupal weight, and emergence were limited to the LC50 concentration. Reductions in both the total number of eggs laid per female and the egg viability were observed with both insecticides at their LC\(_{30}\) and LC\(_{50}\) concentrations. Both female calling activity and the sex pheromone (Z11-hexadecenyl acetate and hexadecenyl acetate) titer were significantly reduced by chlorantraniliprole in LC\(_{50}\) concentration. Antennal responses of female antennae to benzaldehyde and 3-octanone were significantly weaker than controls after exposure to the indoxocarb LC\(_{50}\) concentration. Significant reductions in the enzymatic activity of glutathione S-transferases, mixed-function oxidases, and carboxylesterases were observed in response to both insecticides.
Modern lifestyle is often at odds with endogenously driven rhythmicity, which can lead to circadian disruption and metabolic syndrome. One signature for circadian disruption is a reduced or altered metabolite cycling in the circulating tissue reflecting the current metabolic status. Drosophila is a well-established model in chronobiology, but day-time dependent variations of transport metabolites in the fly circulation are poorly characterized. Here, we sampled fly hemolymph throughout the day and analyzed diacylglycerols (DGs), phosphoethanolamines (PEs) and phosphocholines (PCs) using LC-MS. In wild-type flies kept on sugar-only medium under a light-dark cycle, all transport lipid species showed a synchronized bimodal oscillation pattern with maxima at the beginning and end of the light phase which were impaired in period01 clock mutants. In wild-type flies under constant dark conditions, the oscillation became monophasic with a maximum in the middle of the subjective day. In strong support of clock-driven oscillations, levels of the targeted lipids peaked once in the middle of the light phase under time-restricted feeding independent of the time of food intake. When wild-type flies were reared on full standard medium, the rhythmic alterations of hemolymph lipid levels were greatly attenuated. Our data suggest that the circadian clock aligns daily oscillations of DGs, PEs, and PCs in the hemolymph to the anabolic siesta phase, with a strong influence of light on phase and modality.