Refine
Has Fulltext
- yes (194)
Is part of the Bibliography
- yes (194)
Year of publication
Document Type
- Journal article (173)
- Conference Proceeding (8)
- Doctoral Thesis (7)
- Preprint (6)
Language
- English (194) (remove)
Keywords
- PET (29)
- positron emission tomography (25)
- Positronen-Emissions-Tomografie (21)
- prostate cancer (19)
- theranostics (15)
- CXCR4 (13)
- PET/CT (13)
- PRRT (11)
- PSMA (11)
- SPECT (11)
- multiple myeloma (10)
- neuroendocrine tumor (9)
- dosimetry (8)
- molecular imaging (8)
- radioligand therapy (8)
- SSTR (6)
- 18F-FDG (5)
- 53BP1 (5)
- RADS (5)
- chemokine receptor (5)
- prostate-specific membrane antigen (5)
- somatostatin receptor (5)
- FDG (4)
- NET (4)
- PSMA-RADS (4)
- Parkinson’s disease (4)
- biokinetics (4)
- inflammation (4)
- lymphoma (4)
- medicine (4)
- norepinephrine transporter (4)
- sympathetic nervous system (4)
- γ-H2AX (4)
- 18F-DCFPyL (3)
- 18F-FDG PET/CT (3)
- 18F-LMI1195 (3)
- DaTscan (3)
- MIBG (3)
- MRI (3)
- Prostate Cancer (3)
- SPECT/CT (3)
- Thyroid cancer (3)
- adrenocortical carcinoma (3)
- ageing (3)
- cardiac (3)
- dopamine (3)
- imaging (3)
- machine learning (3)
- peptide receptor radionuclide therapy (3)
- prognosis (3)
- prostate-specific membrane antigen (PSMA) (3)
- radioiodine therapy (3)
- radionuclide therapy (3)
- radiotherapy (3)
- reporting and data system (3)
- salvage radiotherapy (3)
- vandetanib (3)
- 11C-HED (2)
- 123I-mIBG (2)
- 123I-metaiodobenzylguanidine (2)
- 177Lu (2)
- 18F-FDS (2)
- Biokinetics (2)
- C-X-C motif chemokine receptor 4 (2)
- DNA damage (2)
- DNA repair (2)
- DOTATOC (2)
- Dosimetry (2)
- FDG PET/CT (2)
- Ioflupane (2)
- MAG3 (2)
- Myokarditis (2)
- Neuroendocrine Tumor (2)
- Oncology (2)
- PSMA I&T (2)
- PSMA-PET (2)
- Parkinson (2)
- Parkinson Disease (2)
- Parkinson's disease (2)
- Parkinson-Krankheit (2)
- Positron Emission Tomography (2)
- Positron emission tomography (2)
- Radionuclide Therapy (2)
- SSTR-RADS (2)
- SUV (2)
- Schilddrüsenkrebs (2)
- Stammzelle (2)
- TKI (2)
- Virchow Node (2)
- [177Lu]-DOTATATE/-DOTATOC (2)
- [68Ga] (2)
- [68Ga]PentixaFor (2)
- antidepressant (2)
- biodosimetry (2)
- biomarkers (2)
- bone disease (2)
- brain (2)
- cancer (2)
- cancer treatment (2)
- cardiac innervation imaging (2)
- cardiac nerve (2)
- cardiomyocytes (2)
- children (2)
- diabetes (2)
- diabetic cardiomyopathy (2)
- endoradiotherapy (2)
- fatty acid (2)
- fibroblast activation protein (2)
- follow-up (2)
- glioblastoma multiforme (2)
- guidelines (2)
- heart (2)
- heart failure (2)
- hiPSC-CM (2)
- immunohistochemistry (2)
- in vivo imaging (2)
- induced pluripotent stem cells (2)
- involvement (2)
- isotopes (2)
- kidney (2)
- magnetic resonance imaging (2)
- management (2)
- medullary thyroid carcinoma (2)
- metabolism (2)
- molecular medicine (2)
- myocardial sympathetic innervation imaging (2)
- myocarditis (2)
- nuclear medicine (2)
- personalized medicine (2)
- personalized treatment (2)
- positron emission tomography/computed tomography (2)
- precision medicine (2)
- radial (2)
- radiopharmaceuticals (2)
- relapse (2)
- repair (2)
- risk (2)
- sarcoidosis (2)
- somatostatin receptor (SSTR) (2)
- stem cell therapy (2)
- survival (2)
- thyroid cancer (2)
- tracer (2)
- tumor heterogeneity (2)
- tyrosine kinase inhibitor (2)
- 11C-Hydroxyephedrine (1)
- 11C-Methionine PET/CT (1)
- 11C-hydroxyephedrine (1)
- 123I-Ioflupane (1)
- 177Lu SPECT/CT imaging (1)
- 177Lu-DOTATATE (1)
- 177Lu-DOTATOC (1)
- 18-F-fluorothymidine uptake (1)
- 18F-DCFPL (1)
- 18F-flurpiridaz (1)
- 18FFBnTP (1)
- 1st International Workshop (1)
- 2- deoxy-2-(18F)fluoro-D-glucose (1)
- 2-deoxy-2-(18F)fluoro-D-glucose (1)
- 2-deoxy-2-18F-fluoro-D-sorbitol (1)
- 2015 (1)
- 223Ra (1)
- 224Ra (1)
- 3D printing (1)
- 5-Fluorouracil (1)
- 5IA-SPECT (1)
- 68Ga-DOTANOC (1)
- 68Ga-DOTATATE (1)
- 68Ga-DOTATATE/-TOC (1)
- 68Ga-DOTATOC (1)
- 68Ga-PSMA ligand PET/CT (1)
- 68Ga-Pentixafor PET/CT (1)
- 99mTc-DTPA (1)
- <sup>18</sup>F-FDG (1)
- <sup>68</sup>Ga-Pentixafor (1)
- AI (1)
- Ablation <Medizin> (1)
- Absorbed Doses (1)
- Administered Activities (1)
- Alzheimer’s disease (1)
- American Thyroid Association (1)
- Antibodies (1)
- Antidepressants (1)
- Arginine (1)
- B-cell lymphoma (1)
- BON-1 (1)
- BRAF mutation (1)
- BRAF(V600E) mutation (1)
- BSS directive (1)
- C-11-methionine pet (1)
- CD38 (1)
- CNS cancer (1)
- COVID-19 (1)
- CTCAE (1)
- CTNNB1 (1)
- CXCR4-targeting (1)
- CXCR4/SDF-1 (1)
- CXCR7 (1)
- CYP11B enzymes (1)
- Capicua transcriptional repressor (1)
- Carbon-11 (1)
- Cardiology (1)
- Cardiovascular diseases (1)
- Central venous access (1)
- Chernobyl (1)
- Conjugate arc therapy (1)
- Cushing’s disease (1)
- Cushing’s syndrome (1)
- DCGAN (1)
- DLBCL (1)
- DNA Breaks (1)
- DNA Damage Repair (1)
- DNA double-strand breaks (1)
- DOPA-responsive-dystonia (1)
- DOTA-EB-TATE (1)
- DSB damage (1)
- DSB focus substructure (1)
- DWI (1)
- DYT1 (1)
- Deep learning (1)
- Denoising (1)
- Diagnostic Imaging Exams (1)
- Diagnostic radiopharmaceuticals (1)
- Drug resistance (1)
- Dystonia (1)
- EANM (1)
- EANM dosage card (1)
- ECG (1)
- ECG-gated PET (1)
- Effective dose (1)
- Extramedullary disease (1)
- Extraocular eye muscles (1)
- F-18-FDG PET (1)
- FAP (1)
- FAPI PET/CT (1)
- FDG-PET (1)
- FDG-PET/CT (1)
- FDG-PET/MRI (1)
- FLT-PET (1)
- FV45 (1)
- Fabry Disease (FD) (1)
- GAN (1)
- GCA (1)
- GCH1 (1)
- GI (1)
- Ga-68 (1)
- Ga-68-labelled Peptides (1)
- Ganglia (1)
- Gastrointestinal (1)
- Gb3 and lyso-Gb3 biomarkers (1)
- German population (1)
- Gleason score (1)
- Glomerular filtration (1)
- HFmrEF (1)
- HFpEF (1)
- HMDP hydroxymethylene diphosphonate (1)
- Hamburg (1)
- Heart failure (1)
- Herz (1)
- Hickman catheter (1)
- Highlights Lecture (1)
- Hodgkin-lymphoma (1)
- Hyperkalaemia (1)
- I-131 ablation (1)
- IBA-1 (1)
- ICD (1)
- Image Quality (1)
- Imaging pitfalls (1)
- JR11 (1)
- Journal of Nuclear Cardiology (1)
- KWIC (1)
- Knochendichte (1)
- Knochenstoffwechsel (1)
- Langfristige Prognose (1)
- Levothyroxine (1)
- Lu-177 (1)
- Lutetium (1)
- Lysine (1)
- MDD (1)
- MI-RADS (1)
- MIBG scintigraphy (1)
- MOR202 (1)
- MPI (1)
- MS-18 (1)
- Magnetresonanztomografie (1)
- Matlab (1)
- Medullärer Schilddrüsenkrebs (1)
- Merkel cell carcinoma (1)
- Metaiodobenzylguanidine (1)
- Metastases (1)
- Micronuclei (1)
- Molecular Imaging (1)
- Molecular imaging (1)
- Molekulare Bildgebung (1)
- Monte Carlo (1)
- Multiple myeloma (1)
- Muskelkraft (1)
- Myeloma cells (1)
- Myelomas (1)
- Myocardial perfusion (1)
- Myocardial-perfusion SPECT (1)
- NEC (1)
- NR3C1 (1)
- NVP-BGT226 (1)
- Neuroendocrine (1)
- Neurosciences (1)
- Nierenfunktionsstörung (1)
- Nuclear Medicine (1)
- OPS201 (1)
- OXPHOS (1)
- P-glycoprotein expression (1)
- PET/MR systems (1)
- PMR (1)
- PROMISE (1)
- PSA (1)
- PSA response (1)
- PSMA PET/CT (1)
- PSMA-617 (1)
- PSMA-RADS-3A (1)
- PSMA-RADS-3B (1)
- PSMA-TV (1)
- PSMA-targeted PET (1)
- PSMA‐617 (1)
- Pancreas (1)
- Parkinson disease (1)
- Parkinsonism (1)
- Parkionson's disease (1)
- Pediatric Nuclear Medicine (1)
- Pediatric Patients (1)
- Pediatric malignancy (1)
- Pentixafor (1)
- Phase-II (1)
- Physics and instrumentation (1)
- Pitfall (1)
- Port (1)
- Positron-Emission Tomography (1)
- Positronenemissionstomografie (1)
- Preclinical evaluation (1)
- Primary hyperparathyroidism (pHPT) (1)
- Prostata (1)
- Prostate-cancer (1)
- QGP-1 (1)
- RLT (1)
- ROS (1)
- Ra-224 (1)
- Radiation Protection (1)
- Radiation-associated Cancer Risk (1)
- Radiofluorine (1)
- Radioiod (1)
- Radioiodine (1)
- Radioiodine Therapy (1)
- Radionuclide therapy (1)
- Radiopharmacy (1)
- Radiotracer (1)
- Radium (1)
- Raman micro-spectroscopy (1)
- Rhabdomyosarcoma (1)
- Risk Assessment (1)
- Rodents (1)
- SAH (1)
- SARS-CoV-2 (1)
- SPECT Scanner (1)
- SSTR-PET (1)
- Schilddrüse (1)
- Schilddrüsenhormontherapie (1)
- Simvastatin (1)
- Single Molecule Localization Microscopy (SMLM) (1)
- Single-Photon-Emissions-Computertomographie (1)
- Sodium-Glucose Cotransporters (SGLTs) (1)
- Somatostatin receptor expression (1)
- Standardisierung (1)
- T-Lymphozyten-Rezeptor (1)
- T-cell receptor assay (1)
- T-shaped π-π stacking (1)
- T-shaped π–π stacking (1)
- T1rho (1)
- T1ρ (1)
- TBI (1)
- TSPO (1)
- TT\(_{1rho}\) mapping (1)
- T\(_{1P}\) dispersion (1)
- T\(_{1P}\) mapping (1)
- Targeted therapy (1)
- Tc-99m-MAG3 Scans (1)
- Technetium Tc 99m Sestamibi Rats (1)
- Thrombosis (1)
- Thyroid carcinoma (1)
- Tracer (1)
- Transferrin-positive reticulocytes (1)
- USP28 (1)
- USP8 (1)
- WB-DW-MRI (1)
- Waldeyer’s tonsillar ring (1)
- Wnt (1)
- ZDF rats (1)
- [11C]-Choline PET/CT (1)
- [11C]-Methionine (1)
- [177Lu]/[90Y]PentixaTher (1)
- [177Lu]Lu-PSMA I&T (1)
- [177Lu]PentixaTher (1)
- [18F]FDG PET/CT (1)
- [18F]FDG-PET-CT (1)
- [18F]Fluorodeoxythymidine (1)
- [68Ga]DOTATOC (1)
- [68Ga]Pentixafor (1)
- [90Y]PentixaTher (1)
- [99mTc]-Sestamibi scan (1)
- [\(^{223}\)Ra]RaCl\(_{2}\) (1)
- [\(^{68}\)Ga] Pentixafor (1)
- [\(^{68}\)Ga] pentixafor (1)
- [\(^{68}\)Ga]Ga-FAPI (1)
- [\(^{68}\)Ga]Pentixafor (1)
- \(^{11}\)C-Methionine-PET (1)
- \(^{11}\)C-methionine (1)
- \(^{177}\)Lu (1)
- \(^{177}\)Lu-OPS201 (1)
- \(^{18}\)F (1)
- \(^{18}\)F-DCFPyL PET/CT (1)
- \(^{18}\)F-FDG (1)
- \(^{18}\)F-FDG PET/CT (1)
- \(^{18}\)F-PSMA-1007 (1)
- \(^{18}\)F-fluorodeoxyglucose (1)
- \(^{68}\)Ga (1)
- \(^{68}\)Ga-Pentixafor (1)
- \(^{99m}\)Tc-MAG3 (1)
- abdominal lymph node metastases (1)
- absorbed dose (1)
- absorbed dose to the blood (1)
- accuracy (1)
- acute myeloid leukemia (1)
- acute renal failure (1)
- adenocarcinoma of the lung (1)
- adrenal incidentaloma (1)
- adrenocortical (1)
- adsorption (1)
- advanced stages (1)
- agreement (1)
- alpha particles (1)
- alpha-emitters (1)
- amino acids (1)
- amyloid-β (Aβ) (1)
- analysis (1)
- androgen deprivation therapy (1)
- angiogenesis (1)
- angiotensin II type 1 receptor (1)
- antagonist (1)
- anthropometric measurements (1)
- antidepressants (1)
- areas (1)
- arrhythmia (1)
- artificial intelligence (1)
- association (1)
- attention deficit/hyperactivity disorder (ADHD) (1)
- autoimmune thyroiditis (1)
- autologous transplantation (1)
- autonomic nervous system (1)
- autoradiography (1)
- basal ganglia (1)
- base of support (1)
- benzylguanidine (1)
- beta oscillations (1)
- biological dosimetry (1)
- biomarker (1)
- biosynthesis (1)
- blood (1)
- blood flow (1)
- bone marrow cells (1)
- bone metabolism (1)
- bone mineral density (1)
- bone-marrow (1)
- bone-targeting radiopharmaceuticals (1)
- brain metabolic alterations (1)
- brain tumors (1)
- breast cancer (1)
- buparlisib (1)
- butyrylcholinesterase (1)
- c-MYC (1)
- calcitonin (1)
- cancer associated fibroblasts (1)
- cancer of unknown primary (CUP) (1)
- cancer-associated fibroblast (1)
- carbamate (1)
- carboxylation (1)
- carcinoma (1)
- carcinoma metastases to pancreas (1)
- cardiac neurohormonal system (1)
- cardiac sympathetic nerve system (1)
- cardiac sympathetic nervous system (1)
- cardioprotective potential (1)
- cardiovascular diseases (1)
- caudate nucleus (1)
- cell biology (1)
- cell staining (1)
- cells (1)
- cerebral gliomas (1)
- chemokine receptor-4 (1)
- childhood and adolescence (1)
- cholinergic activity (1)
- chromatin mobility (1)
- clinical diagnosis (1)
- coefficient (1)
- cognitive decline (1)
- coherence analysis (1)
- coherent anti-Stokes Raman scattering (CARS) microscopy (1)
- collagens (1)
- collimator (1)
- combination (1)
- comparability (1)
- complex DNA damage (1)
- computational biology and bioinformatics (1)
- contractility (1)
- contrast agent (1)
- coronary artery disease (1)
- correction (1)
- criteria (1)
- damage (1)
- daratumumab (1)
- data analysis (1)
- delineation (1)
- dementia (1)
- depression (1)
- detection rate (1)
- diagnostic medical radiation exposure (1)
- diagnostics (1)
- diastolic dysfunction (1)
- differentiated thyroid cancer (1)
- differentiated thyroid carcinoma (1)
- diffuse (1)
- diffusion weighted MRI (1)
- diffusion weighted mri (1)
- dilated cardiomyopathy with ataxia (1)
- disease (1)
- dissection (1)
- distant metastases (1)
- dopamine acetylcholine (1)
- dopamine transporter (DAT) (1)
- dose response (1)
- double-stranded (1)
- drug discovery (1)
- early response (1)
- editorial (1)
- ejection fraction (1)
- endocrinology (1)
- enzyme kinetics (1)
- epidemiology (1)
- esophagogastric junction (1)
- evans blue (1)
- exome sequencing (1)
- experience (1)
- exposure (1)
- extracellular matrix (1)
- extramedullary hematopoiesis (1)
- flare phenomenon (1)
- fluorine-18 (1)
- focal (1)
- focused surgical approach (1)
- folinic acid (1)
- follicular lymphoma (1)
- free‐breathing (1)
- gait initiation (1)
- gamma rays (1)
- gefitinib (1)
- gemcitabine (1)
- genetics (1)
- giant cell arteritis (1)
- glioblastoma (1)
- glioma (1)
- glomerular filtration rate (1)
- glucocorticoid excess (1)
- harmonization of SPECT/CT imaging (1)
- head and neck cancer (1)
- health care (1)
- healthy volunteers (1)
- heart failure with mid-range ejection fraction (1)
- heart-to-mediastinum ratio (1)
- hematotoxicity (1)
- heptacellular carcinoma (1)
- high LET irradiation (1)
- high risk (1)
- histone H2AX (1)
- humans (1)
- hydroxyephedrine (1)
- hypercortisolism (1)
- hyperkalemia (1)
- hypothyroidism (1)
- imaging proliferation (1)
- imaging techniques (1)
- immune check inhibitor (1)
- immune infiltration (1)
- immunostaining (1)
- improves (1)
- in vivo formation (1)
- in-vivo (1)
- inhibition (1)
- initial experience (1)
- international multicenter comparison exercise (1)
- interobserver (1)
- interreader (1)
- intraindividual comparison (1)
- iodine contrast (1)
- iodine nutrition (1)
- iodine-131 (1)
- irinotecan (1)
- irradiation (1)
- isotopic labelling (1)
- kidney function (1)
- kinase inhibitor (1)
- late response (1)
- left-ventricular function (1)
- lesions (1)
- leukocytes (1)
- levodopa-induced dyskinesia (1)
- linear conversion (1)
- locally advanced disease (1)
- long-term complications (1)
- long-term outcome (1)
- lung (1)
- lung and intrathoracic tumors (1)
- lung cancer (1)
- lutetium-177 (1)
- mIBG (1)
- mRNA (1)
- macrophages (1)
- macroscopic recurrence (1)
- major depressive disorder (1)
- malignancies (1)
- malignant lymphoma (1)
- mammalian target of rapamycin (1)
- mapping (1)
- matched pair (1)
- mechanisms retention (1)
- medium-sized animals (1)
- medullary thyroid cancer (1)
- meningioma (1)
- metabolic tumor volume (MTV) (1)
- metabolic tumour volume (MTV) (1)
- metastasis-directed therapy (1)
- methionine (1)
- methionine pet (1)
- methylation (1)
- methylphenidate (1)
- miRNA (1)
- mice (1)
- microenvironment (1)
- microglial cells (1)
- mitochondria (1)
- molecular biology (1)
- molecular diagnostics (1)
- molecular radiotherapy (1)
- molecular radiotherapy (MRT) (1)
- motor control (1)
- mouse (1)
- movement disorders (1)
- moycardial sympathetic innervation (1)
- multi-pinhole collimation (1)
- multiple system atrophy (1)
- multivariate data analysis (1)
- muscle force (1)
- myocardial nerve (1)
- myocardial perfusion imaging (1)
- nab-paclitaxel (1)
- neoadjuvant chemotherapy (1)
- nephrology (1)
- nephrotoxicity (1)
- neuroblastoma (1)
- neuroendocrine neoplasia (1)
- neuroendocrine neoplasms (NEN) (1)
- neuroendocrine tumor (NET) (1)
- neuroendocrine tumors (1)
- neuroendocrine tumors (NET) (1)
- neuroinflammation (1)
- neurology (1)
- nicotinic receptors (1)
- nitrate and thyroid carcinogenesis (1)
- non-Hodgkin's lymphoma (1)
- non-hodgkins-lymphoma (1)
- nonhuman primates (1)
- nuclear cardiology (1)
- nuclear medicine therapy (1)
- oligorecurrence (1)
- ollimator (1)
- optimization (1)
- organic cation transporter (1)
- osteoporosis (1)
- other radiation exposure (atomic bombing/nuclear accidents) (1)
- outcomes research (1)
- overall survival (1)
- oxaliplatin (1)
- pancreas (1)
- pancreatic cancer (1)
- papillary (1)
- papillary thyroid carcinoma (PTC) (1)
- parathyroid adenoma (1)
- parathyroid carcinoma (1)
- pattern (1)
- pediatric patients (1)
- pediatric thyroid cancer after Chernobyl and Fukushima (1)
- pembrolizumab (1)
- peptide receptor (1)
- performance (1)
- performance evaluation (1)
- peripheral injury (1)
- peripheral nervous system (1)
- phaeochromocytoma (1)
- phantom (1)
- phenethylguanidine (1)
- phosphatidylinositol-3-kinase (1)
- phosphorylation (1)
- photons (1)
- pig model (1)
- pleural mesothelioma (1)
- polymyalgia rheumatica (1)
- pooled (1)
- positron-emission-tomography (1)
- post-reconstruction filtering (1)
- power-station accident (1)
- preclinical PET (1)
- preclinical imaging (1)
- prediction (1)
- preoperative localization (1)
- primary hyperparathyroidism (1)
- prognostic value (1)
- progression (1)
- prostate-specific antigen (1)
- pulmonary imaging (1)
- quality (1)
- quantification (1)
- quantitative MRI (1)
- quantitative SPECT/CT (1)
- quantitative imaging (1)
- radiation (1)
- radiation effects (1)
- radiobiology (1)
- radiochemistry (1)
- radiogenomics (1)
- radioiodine (1)
- radiotracer (1)
- radiotracer kinetics (1)
- radiotracers (1)
- radium (1)
- rats (1)
- recurrence (1)
- recurrent prostate cancer (1)
- refractory (1)
- renal (1)
- renal failure (1)
- renal function (1)
- renal imaging (1)
- renal scintigraphy (1)
- renin-angiotensin system (1)
- repeat surgery (1)
- repeated surgery (1)
- reporting and data systems (1)
- response evaluation (1)
- responsivity (1)
- rising incidence of thyroid cancer (1)
- risk assessment (1)
- scanner (1)
- screening and overdiagnosis (1)
- second hit (1)
- second primary malignancy (1)
- secondary lung tumors (1)
- self‐gated (1)
- selpercatinib (1)
- sepsis (1)
- sigma-1 receptor-directed molecular imaging (1)
- signal to noise ratio (1)
- signaling pathway (1)
- simultaneous integrated boost (1)
- single photon emission computed tomography (SPECT) (1)
- single photon emission computed tomography: sympathetic nerve (1)
- skeletal (1)
- skin biopsy (1)
- skin hemagioma (1)
- small animal (1)
- small animal SPECT (1)
- small-animal SPECT (1)
- small-animal imaging (1)
- smoldering myeloma (1)
- software (1)
- solid tumors (1)
- somatostatin (1)
- somatostatin receptors (1)
- sorbents (1)
- spin lock (1)
- spin-lock (1)
- spleen (1)
- split renal function (1)
- staging (1)
- standardization (1)
- standardization of SPECT/CT imaging (1)
- standardized reporting (1)
- standardized reporting system (1)
- statin (1)
- stem cells (1)
- stem-cell research (1)
- stem-cell transplantation (1)
- stimulation (1)
- storage vesicle turnover (1)
- striatum (1)
- stroke (1)
- structure–activity relationships (1)
- subthalamic nucleus (1)
- super ultraviolet (1)
- surgery (1)
- surgical treatment (1)
- sympathetic nerve (1)
- target (1)
- taxane (1)
- therapeutic medical radiation exposure (EBRT/ RAI) (1)
- therapeutic target (1)
- thyroid (1)
- thyroid carcinoma (TC) (1)
- thyroid carcinomas (1)
- total lesion PSMA (1)
- total lesion glycolysis (TLG) (1)
- total lesion methionine uptake (TLMU) (1)
- traceability of SPECT/CT imaging (1)
- trachea (1)
- transcriptome (1)
- treatment (1)
- treatment response (1)
- trial (1)
- tumor (1)
- tumor burden (1)
- tumor microenvironment (1)
- unilateral ureteral obstruction (1)
- urology (1)
- valsartan (1)
- various cancer diseases (1)
- vasculature (1)
- vasculitis (1)
- vemurafenib (1)
- vestibular schwannoma (1)
- wave‐CAIPI (1)
- weight drop (1)
- whole body MRI (1)
- whole-body (1)
- young females (1)
- α-Emitter (1)
- α-Particle (1)
- α-emitter (1)
- β-catenin (1)
- γ-h2ax (1)
Institute
- Klinik und Poliklinik für Nuklearmedizin (194) (remove)
Sonstige beteiligte Institutionen
- Johns Hopkins School of Medicine (17)
- Johns Hopkins University School of Medicine (5)
- Johns Hopkins School of Medicine, Baltimore, MD, U.S. (4)
- Department of Biomedical Imaging, National Cerebral and Cardiovascular Research Center, Suita, Japan (2)
- Division of Medical Technology and Science, Department of Medical Physics and Engineering, Course of Health Science, Osaka University Graduate School of Medicine, Suita Japan (2)
- Institut for Molecular Biology and CMBI, Department of Genomics, Stem Cell Biology and Regenerative Medicine, Leopold-Franzens-University Innsbruck, Innsbruck, Austria (2)
- Johns Hopkins School of Medicine, The Russell H Morgan Department of Radiology and Radiological Science, Baltimore, MD, USA (2)
- Bundeswehr Institute of Radiobiology affiliated to the University of Ulm, Munich, Germany (1)
- CAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior - the development agency of the Brazilian Federal Government (1)
- DAAD - Deutscher Akademischer Austauschdienst (1)
Highlights
• Loss of DNAJC19's DnaJ domain disrupts cardiac mitochondrial structure, leading to abnormal cristae formation in iPSC-CMs.
• Impaired mitochondrial structures lead to an increased mitochondrial respiration, ROS and an elevated membrane potential.
• Mutant iPSC-CMs show sarcomere dysfunction and a trend to more arrhythmias, resembling DCMA-associated cardiomyopathy.
Background
Dilated cardiomyopathy with ataxia (DCMA) is an autosomal recessive disorder arising from truncating mutations in DNAJC19, which encodes an inner mitochondrial membrane protein. Clinical features include an early onset, often life-threatening, cardiomyopathy associated with other metabolic features. Here, we aim to understand the metabolic and pathophysiological mechanisms of mutant DNAJC19 for the development of cardiomyopathy.
Methods
We generated induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) of two affected siblings with DCMA and a gene-edited truncation variant (tv) of DNAJC19 which all lack the conserved DnaJ interaction domain. The mutant iPSC-CMs and their respective control cells were subjected to various analyses, including assessments of morphology, metabolic function, and physiological consequences such as Ca\(^{2+}\) kinetics, contractility, and arrhythmic potential. Validation of respiration analysis was done in a gene-edited HeLa cell line (DNAJC19tv\(_{HeLa}\)).
Results
Structural analyses revealed mitochondrial fragmentation and abnormal cristae formation associated with an overall reduced mitochondrial protein expression in mutant iPSC-CMs. Morphological alterations were associated with higher oxygen consumption rates (OCRs) in all three mutant iPSC-CMs, indicating higher electron transport chain activity to meet cellular ATP demands. Additionally, increased extracellular acidification rates suggested an increase in overall metabolic flux, while radioactive tracer uptake studies revealed decreased fatty acid uptake and utilization of glucose. Mutant iPSC-CMs also showed increased reactive oxygen species (ROS) and an elevated mitochondrial membrane potential. Increased mitochondrial respiration with pyruvate and malate as substrates was observed in mutant DNAJC19tv HeLa cells in addition to an upregulation of respiratory chain complexes, while cellular ATP-levels remain the same. Moreover, mitochondrial alterations were associated with increased beating frequencies, elevated diastolic Ca\(^{2+}\) concentrations, reduced sarcomere shortening and an increased beat-to-beat rate variability in mutant cell lines in response to β-adrenergic stimulation.
Conclusions
Loss of the DnaJ domain disturbs cardiac mitochondrial structure with abnormal cristae formation and leads to mitochondrial dysfunction, suggesting that DNAJC19 plays an essential role in mitochondrial morphogenesis and biogenesis. Moreover, increased mitochondrial respiration, altered substrate utilization, increased ROS production and abnormal Ca\(^{2+}\) kinetics provide insights into the pathogenesis of DCMA-related cardiomyopathy.
Analysis of cerebral glucose metabolism following experimental subarachnoid hemorrhage over 7 days
(2023)
Little is known about changes in brain metabolism following SAH, possibly leading towards secondary brain damage. Despite sustained progress in the last decade, analysis of in vivo acquired data still remains challenging. The present interdisciplinary study uses a semi-automated data analysis tool analyzing imaging data independently from the administrated radiotracer. The uptake of 2-[18F]Fluoro-2-deoxy-glucose ([\(^{18}\)F]FDG) was evaluated in different brain regions in 14 male Sprague–Dawley rats, randomized into two groups: (1) SAH induced by the endovascular filament model and (2) sham operated controls. Serial [\(^{18}\)F]FDG-PET measurements were carried out. Quantitative image analysis was performed by uptake ratio using a self-developed MRI-template based data analysis tool. SAH animals showed significantly higher [\(^{18}\)F]FDG accumulation in gray matter, neocortex and olfactory system as compared to animals of the sham group, while white matter and basal forebrain region showed significant reduced tracer accumulation in SAH animals. All significant metabolic changes were visualized from 3 h, over 24 h (day 1), day 4 and day 7 following SAH/sham operation. This [\(^{18}\)F]FDG-PET study provides important insights into glucose metabolism alterations following SAH—for the first time in different brain regions and up to day 7 during course of disease.
[\(^{223}\)Ra]RaCl\(_2\) and [\(^{224}\)Ra]RaCl\(_2\) are bone seekers, emitting high LET, and short range (< 100 μm) alpha-particles. Both radionuclides show similar decay properties; the total alpha energies are comparable (\(^{223}\)Ra: ≈28 MeV, \(^{224}\)Ra: ≈26 MeV). [\(^{224}\)Ra]RaCl\(_2\) has been used from the mid-1940s until 1990 for treating different bone and joint diseases with activities of up to approximately 50 MBq [\(^{224}\)Ra]RaCl\(_2\). In 2013 [\(^{223}\)Ra]RaCl\(_2\) obtained marketing authorization by the FDA and by the European Union for the treatment of metastatic prostate cancer with an activity to administer of 0.055 MBq per kg body weight for six cycles. For intravenous injections in humans a model calculation using the biokinetic model of ICRP67 shows a ratio of organ absorbed dose coefficients (\(^{224}\)Ra:\(^{223}\)Ra) between 0.37 (liver) and 0.97 except for the kidneys (2.27) and blood (1.57). For the red marrow as primary organ-at-risk, the ratio is 0.57. The differences are mainly caused be the differing half-lives of the decay products of both radium isotopes. Both radionuclides show comparable DNA damage patterns in peripheral blood mononuclear cells after internal ex-vivo irradiation. Data on the long-term radiation-associated side effects are only available for treatment with [\(^{224}\)Ra]RaCl\(_2\). Two epidemiological studies followed two patient groups treated with [\(^{224}\)Ra]RaCl\(_2\) for more than 25 years. One of them was the “Spiess study”, a cohort of 899 juvenile patients who received several injections of [\(^{224}\)Ra]RaCl\(_2\) with a mean specific activity of 0.66 MBq/kg. Another patient group of ankylosing spondylitis patients was treated with 10 repeated intravenous injections of [\(^{224}\)Ra]RaCl\(_2\), 1 MBq each, 1 week apart. In total 1,471 of these patients were followed-up in the “Wick study”. In both studies, an increased cancer mortality by leukemia and solid cancers was observed. Similar considerations on long-term effects likely apply to [\(^{223}\)Ra]RaCl\(_2\) as well since the biokinetics are similar and the absorbed doses in the same range. However, this increased risk will most likely not be observed due to the much shorter life expectancy of prostate cancer patients treated with [\(^{223}\)Ra]RaCl\(_2\).
(1) Background: C-X-C Motif Chemokine Receptor 4 (CXCR4) and Fibroblast Activation Protein Alpha (FAP) are promising theranostic targets. However, it is unclear whether CXCR4 and FAP positivity mark distinct microenvironments, especially in solid tumors. (2) Methods: Using Random Forest (RF) analysis, we searched for entity-independent mRNA and microRNA signatures related to CXCR4 and FAP overexpression in our pan-cancer cohort from The Cancer Genome Atlas (TCGA) database — representing n = 9242 specimens from 29 tumor entities. CXCR4- and FAP-positive samples were assessed via StringDB cluster analysis, EnrichR, Metascape, and Gene Set Enrichment Analysis (GSEA). Findings were validated via correlation analyses in n = 1541 tumor samples. TIMER2.0 analyzed the association of CXCR4 / FAP expression and infiltration levels of immune-related cells. (3) Results: We identified entity-independent CXCR4 and FAP gene signatures representative for the majority of solid cancers. While CXCR4 positivity marked an immune-related microenvironment, FAP overexpression highlighted an angiogenesis-associated niche. TIMER2.0 analysis confirmed characteristic infiltration levels of CD8+ cells for CXCR4-positive tumors and endothelial cells for FAP-positive tumors. (4) Conclusions: CXCR4- and FAP-directed PET imaging could provide a non-invasive decision aid for entity-agnostic treatment of microenvironment in solid malignancies. Moreover, this machine learning workflow can easily be transferred towards other theranostic targets.
Molecular imaging of rats is of great importance for basic and translational research. As a powerful tool in nuclear medicine, SPECT can be used to visualize specific functional processes in the body, such as myocardial perfusion or bone metabolism. Typical applications in laboratory animals are imaging diagnostics or the development of new tracers for clinical use. Innovations have enabled resolutions of up to a quarter of a millimeter with acceptable sensitivity. These advances have recently led to significantly more interest in SPECT both clinically and preclinically.
The objective of this thesis was to evaluate the performance of the new U-SPECT5/CT E-Class by MILabs with a dedicated ultra-high resolution multi-pinhole collimator for rats and its potential for in vivo imaging of rats. The unique features of the U-SPECT are the large stationary detectors and the new iterative reconstruction algorithm. In addition, compared to the conventional system, the "E-Class" uses only two detectors instead of three.
First, the sensitivity, maximum resolution, and uniformity were determined as performance parameters. Thereafter, CNRs for different activity levels comparable to those of typical in vivo activities were examined. Finally, two example protocols were carried out for imaging with 99mTc-MIBI and 99mTc-HMDP in healthy rats to evaluate the in vivo capabilities. For this purpose, CNR calculations and an image quality assessment were performed. The focus was on image quality as a function of scan time and post-reconstruction filter across a wide range of realistically achievable in vivo conditions.
Performance was reasonable compared to other systems in the literature, with a sensitivity of 567 cps/MBq, a maximum resolution of 1.20 mm, and a uniformity of 55.5%. At the lower activities, resolution in phantom studies decreased to ≥1.80 mm while maintaining good image quality. High-quality bone and myocardial perfusion SPECTs were obtained in rats with a resolution of ≥1.80 mm and ≥2.20 mm, respectively. Although limited sensitivity remains a weakness of SPECT, the U-SPECT5/CT E-Class with the UHR-RM collimator can achieve in vivo results of the highest standard despite the missing third detector. Currently, it is one of the best options for high-resolution radionuclide imaging in rats.
SPECT as a representative of molecular imaging allows visualization of metabolic processes in vivo. In clinical practice, single photon emission imaging is an established modality for myocardial perfusion imaging or the diagnosis of adrenal or neuroendocrine tumors, to name a few. With technical advances in scanner design and data processing leading to improved spatial resolution and image quality, SPECT has become a serious contender in small animal preclinical imaging. With multi-pinhole collimation, submillimeter spatial resolutions are achieved without limiting sensitivity, which has led to a significant increase of interest in SPECT for preclinical research in recent years.
In this dissertation, the potential of a two-detector system through an analysis of three dedicated mouse collimators with multi-pinhole configurations was demonstrated. For this, sensitivity, spatial resolution, and uniformity as key parameters were determined. In the second part of the present work, an evaluation of the image quality at different activity concentrations to allow prediction of the system performance related to in vivo studies was performed. Therefore, a visual evaluation, as well as a calculation of the contrastto-noise ratio, was performed using mini Derenzo phantoms for the respective three mouse collimators. To better classify the results, the study was extended by a comparison with the predecessor system.
Due to the absence of the third bottom detector, sensitivity and uniformity are slightly compromised. All three collimators were able to achieve a spatial resolution in the submillimeter range, XUHR-M offers a peak resolution of up to 0.35 mm. In terms of resolution, both evaluated systems performed on an equal level. Visual assessment of image quality indicates a slight advantage of the new two-detector system, and the contrast-to-noise ratio seems to benefit from the improved SROSEM algorithm. However, the differences between the two systems are marginal.
The U-SPECT5/CT E-Class is proven to be state-of-the-art for small animal imaging and is a powerful instrument for preclinical molecular imaging research. Improvements in system design compensate well for the reduction in the detection area, allowing excellent imaging even with low activity concentrations.
Purpose: A new PET radiotracer \(^{18}\)F-AF78 showing great potential for clinical application has been reported recently. It belongs to a new generation of phenethylguanidine-based norepinephrine transporter (NET)-targeting radiotracers. Although many efforts have been made to develop NET inhibitors as antidepressants, systemic investigations of the structure–activity relationships (SARs) of NET-targeting radiotracers have rarely been performed. Methods: Without changing the phenethylguanidine pharmacophore and 3-fluoropropyl moiety that is crucial for easy labeling, six new analogs of \(^{18}\)F-AF78 with different meta-substituents on the benzene-ring were synthesized and evaluated in a competitive cellular uptake assay and in in vivo animal experiments in rats. Computational modeling of these tracers was established to quantitatively rationalize the interaction between the radiotracers and NET. Results: Using non-radiolabeled reference compounds, a competitive cellular uptake assay showed a decrease in NET-transporting affinity from meta-fluorine to iodine (0.42 and 6.51 µM, respectively), with meta-OH being the least active (22.67 µM). Furthermore, in vivo animal studies with radioisotopes showed that heart-to-blood ratios agreed with the cellular experiments, with AF78(F) exhibiting the highest cardiac uptake. This result correlates positively with the electronegativity rather than the atomic radius of the meta-substituent. Computational modeling studies revealed a crucial influence of halogen substituents on the radiotracer–NET interaction, whereby a T-shaped π–π stacking interaction between the benzene-ring of the tracer and the amino acid residues surrounding the NET binding site made major contributions to the different affinities, in accordance with the pharmacological data. Conclusion: The SARs were characterized by in vitro and in vivo evaluation, and computational modeling quantitatively rationalized the interaction between radiotracers and the NET binding site. These findings pave the way for further evaluation in different species and underline the potential of AF78(F) for clinical application, e.g., cardiac innervation imaging or molecular imaging of neuroendocrine tumors.
Background: The American Thyroid Association (ATA) uses criteria to assess the risk for persistent disease in differentiated thyroid carcinoma (DTC) after radioiodine therapy (RAI). There are no data available showing that this classification can be adopted unadjusted by Germany. Aim: The aim of our study is to investigate whether the ATA classification can be applied to a German population for short-term prognosis. Furthermore, we investigated the influence of an age cutoff value. Methods: We retrospectively analyzed 121 patients who were referred to our tertiary referral center. Patients were classified into risk categories, and the therapy response was determined according to ATA. Results: A total of 73/83 (88%) ATA low-risk patients and 12/19 (63%) intermediate-risk patients showed an excellent response; 2/19 (11%) high-risk patients had a biochemical, and 6 (31%) had a structural incomplete response. Of all 39 patients ≥55 years, 84% had an excellent response. Using a cut off of 50 years, 50/62 (81%) of the older patients showed an excellent response. Conclusion: The ATA risk classification is able to estimate the response to RAI therapy in a German population. A shift from 55 to 50 years as an age cutoff value does not result in any relevant change in the treatment response.