Refine
Is part of the Bibliography
- yes (31)
Year of publication
- 2017 (31) (remove)
Document Type
- Doctoral Thesis (31)
Language
- English (31) (remove)
Keywords
- Motoneuron (3)
- Ackerschmalwand (2)
- Bioinformatics (2)
- Metagenom (2)
- Soziale Insekten (2)
- Spinale Muskelatrophie (2)
- Thrombozyt (2)
- Transkriptionsfaktor (2)
- Acetylation (1)
- Acetylierung (1)
- Actin (1)
- Actin Dynamics (1)
- Actinomyces (1)
- Actinomycetes (1)
- Adipogenesis (1)
- Angiogenese (1)
- Antiangiogenese (1)
- Aplysina aerophoba (1)
- Arabidopsis thaliana (1)
- Arbeitsteilung (1)
- Autotransporter (1)
- Axon (1)
- Axon Branching (1)
- B7-H1 (1)
- BMP-2 (1)
- Bacteria (1)
- Bakterien (1)
- Basalmembran (1)
- Basement membrane (1)
- Bestäubung (1)
- Bestäubung Pollination Honigbiene Hummel (1)
- Biene (1)
- Bienen Dressur Lernen (1)
- Bipolar (1)
- Bone marrow stromal cell (1)
- Bovine Mastitis (1)
- CNBP (1)
- Cataglyphis (1)
- Chaetomium thermophilum (1)
- Collagen gels (1)
- DNA-Reparatur (1)
- DREAM complex (1)
- Deep sequencing (1)
- Dereplicaiton (1)
- Entzündung (1)
- Epithelial-mesenchymale Transition (1)
- Escherichia coli (1)
- Farbensehen (1)
- Fettzelle (1)
- Fibroblastenwachstumsfaktor (1)
- GPCR (1)
- General Transcription Factor II H (1)
- Genexpression (1)
- Genomics (1)
- Glycoprotein hormone (1)
- Golgi apparatus (1)
- Golgi-Apparat (1)
- Hemostasis (1)
- Himmelskompass (1)
- Hitzestress (1)
- Hyaliner Knorpel (1)
- IEG (1)
- Illumina HiSeq (1)
- Immunotherapy (1)
- Inflammation (1)
- Isomer (1)
- K-Ras (1)
- Knochenbildung (1)
- Knochenmarkzelle (1)
- L-typ Calciumkanal Antagonist (1)
- LC-MS (1)
- Langzeitgedächtnis (1)
- Lipidumbau (1)
- Lungenkrebs (1)
- MMB (1)
- Marine natural products (1)
- Marine sponges (1)
- Mass Spectrometry (1)
- Meeresschwämme (1)
- Megakaryopoese (1)
- Metabolomics (1)
- Metabolomik (1)
- Metagenomics (1)
- Mikroorganismus (1)
- Mitose (1)
- Motoneurons (1)
- Multiple Sklerose (1)
- Natural products (1)
- Nervensystem (1)
- Neuroethologie (1)
- Neuromodulation (1)
- Neuronale Plastizität (1)
- Neuropeptide (1)
- Neuropeptidom (1)
- Nicht-kleinzelliges Bronchialkarzinom (NSCLC) (1)
- Nimodipin (1)
- Osteogenesis (1)
- PKA signaling (1)
- PTMs (1)
- PacBio sequencing (1)
- Phosphorylation (1)
- Phosphorylierung (1)
- Phyllosphere (1)
- Phyllosphäre (1)
- Pilzkörper (1)
- Platelet granules (1)
- Platelets (1)
- Posttranslationale Änderung (1)
- Protein chemistry (1)
- Quantifizierung (1)
- Quantitation (1)
- RNA binding potein CNBP (1)
- Receptor internalization (1)
- Receptor signaling (1)
- Riesensynapsen (1)
- SMN (1)
- STEC (1)
- Sap47 (1)
- Schlaganfall (1)
- Schwämme (1)
- Sekundärmetabolit (1)
- Skin (1)
- Spinal Muscular Atrophy (1)
- Sponges (1)
- Ssl1 (1)
- Staphylococcus (1)
- Stroke (1)
- Syap1 (1)
- Synapse (1)
- Synapse-associated protein (1)
- Synapsen assoziiert (1)
- TFIIH (1)
- Test system (1)
- Tfb4 (1)
- Thermotoleranz (1)
- Thrombosis (1)
- Thrombozyten (1)
- Thrombozytenaggregation (1)
- Thrombozytopathie (1)
- Thrombozytopoese (1)
- Tissue Engineering (1)
- Transkription (1)
- Triglyceride (1)
- Tumor (1)
- Tumorangiogenese (1)
- Tumorgefäßmorphologie (1)
- U snRNPs (1)
- Verhaltensplastizität (1)
- Zellskelett (1)
- Zytoskelett (1)
- alkaloids (1)
- amyotrophic lateral sclerosis (1)
- autotransporter (1)
- bac-genomics-scripts (1)
- cAMP signaling (1)
- cartilage regeneration (1)
- differential coverage binning (1)
- ecoli_VF_collection (1)
- endophyte (1)
- entero-aggregative-haemorrhagic Escherichia coli (EAHEC) (1)
- epithelial-mesenchymal transition (1)
- experimental autoimmune encephalomyelitis (1)
- experimentelle autoimmune Enzephalomyelitis (1)
- fungal endophytes of grasses (1)
- gonococcal (1)
- gonococcal infection (1)
- grass (1)
- high-throughput sequencing (1)
- homeostasis (1)
- hyaline cartilage (1)
- hybrid assembly (1)
- immune system (1)
- inflammation (1)
- insect visual learning (1)
- l-type calcium channel antagonist (1)
- lipid remodeling (1)
- long-term memory formation (1)
- lung cancer (1)
- mastitis-associated Escherichia coli (MAEC) (1)
- megakaryocyte (1)
- metagenomics (1)
- miR-21 (1)
- miRNA (1)
- mitotic gene expression (1)
- multiple sclerosis (1)
- mushroom body calyx microglomeruli (1)
- neuronal activation (1)
- neuropathic pain (1)
- neuroprotection (1)
- p34 (1)
- p44 (1)
- pICln (1)
- pathotypes (1)
- phylogeny (1)
- plant defense (1)
- platelet aggregation (1)
- protein immobilization (1)
- single-cell genomics (1)
- site-specific immobilization (1)
- smell (1)
- smells (1)
- social cognition (1)
- social interaction (1)
- social stimuli (1)
- sphingolipids (1)
- sponge microbiome (1)
- symbiosis (1)
- thermotolerance (1)
- tumor angiogenesis (1)
- tumor vascular morphologie (1)
- virulence factors (1)
- visuelles Langzeitgedächtnis (1)
Institute
- Graduate School of Life Sciences (31) (remove)
Sonstige beteiligte Institutionen
The Role of DREAM/MMB-mediated mitotic gene expression downstream of mutated K-Ras in lung cancer
(2017)
The evolutionary conserved Myb-MuvB (MMB) multiprotein complex has an essential role in transcriptional activation of mitotic genes. MMB target genes as well as the MMB associated transcription factor B-Myb and FoxM1 are highly expressed in a range of different cancer types. The elevated expression of these genes correlates with an advanced tumor state and a poor prognosis. This suggests that MMB could contribute to tumorigenesis by mediating overexpression of mitotic genes. Although MMB has been extensively characterized biochemically, the requirement for MMB to tumorigenesis in vivo remains largely unknown and has not been tested directly so far.
In this study, conditional knockout of the MMB core member Lin9 inhibits tumor formation in vivo in a mouse model of lung cancer driven by oncogenic K-Ras and loss of p53. The incomplete recombination observed within tumors points towards an enormous selection pressure against the complete loss of Lin9. RNA interference (RNAi)-mediated depletion of Lin9 or the MMB associated subunit B-Myb provides evidence that MMB is required for the expression of mitotic genes in lung cancer cells. Moreover, it was demonstrated that proliferation of lung cancer cells strongly depends on MMB. Furthermore, in this study, the relationship of MMB to the p53 tumor suppressor was investigated in a primary lung cancer cell line with restorable p53 function. Expression analysis revealed that mitotic genes are downregulated after p53 re-expression. Moreover, activation of p53 induces formation of the repressive DREAM complex and results in enrichment of DREAM at mitotic gene promoters. Conversely, MMB is displaced at these promoters.
Based on these findings the following model is proposed: In p53-negative cells, mitogenic stimuli foster the switch from DREAM to MMB. Thus, mitotic genes are overexpressed and may promote chromosomal instability and tumorigenesis.
This study provides evidence that MMB contributes to the upregulation of G2/M phase-specific genes in p53-negative cells and suggests that inhibition of MMB (or its target genes) might be a strategy for treatment of lung cancer.