Refine
Has Fulltext
- yes (52)
Is part of the Bibliography
- yes (52)
Year of publication
Document Type
- Journal article (52) (remove)
Language
- German (52) (remove)
Keywords
- Physiologische Chemie (3)
- Zoologie (2)
- Abbe-Limit (1)
- Ameisen (1)
- Baum (1)
- Biene (1)
- Bienenwolf (1)
- Cataglyphis-Wüstenameisen (1)
- Chromatin (1)
- Elektrofusion (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (52) (remove)
The diffraction limit of light confines fluorescence imaging of subcellular structures in fungi. Different super-resolution methods are available for the analysis of fungi that we briefly discuss. We exploit the filamentous fungus Fusarium fujikuroi expressing a YFP-labeled membrane protein showing the benefit of correlative light- and electron microscopy (CLEM), that combines structured illumination microscopy (SIM) and scanning election microscopy (SEM).
Advanced tissue imaging techniques and super resolution microscopy are opening new avenues of investigations in life sciences. These mainly instrumentation-driven innovations require the development of appropriate molecular labelling tools. Here, we discuss currently used and upcoming manipulation-free protein labelling strategies and their potential for the precise and interference-free visualization of endogenous proteins.