Refine
Has Fulltext
- yes (56)
Is part of the Bibliography
- yes (56)
Year of publication
Document Type
- Journal article (45)
- Conference Proceeding (10)
- Other (1)
Language
- English (56) (remove)
Keywords
- Chirurgie (24)
- Immunbiologie (7)
- Immunobiologie (4)
- Langerhans-Inseln (2)
- Transplantation (2)
- enzyme activation (2)
- enzyme inhibitors (2)
- islets of Langerhans (2)
- rat (2)
- swine (2)
- 15-Deoxyspergualin (1)
- Allergie (1)
- Class II antigen blockade (1)
- Dünndarm (1)
- Göttingen (1)
- Heterotransplantation (1)
- Immunologie (1)
- MHC Klasse II (1)
- Makrophage (1)
- Minischwein (1)
- Monoclonal antibody (1)
- Niere (1)
- Ratte (1)
- beta cells (1)
- bone marrow (1)
- dass II antigen blockade (1)
- diabetes (1)
- experimental therapy (1)
- hematology (1)
- immunosuppression (1)
- intravenous glucose tolerance test (1)
- islet transplantation (1)
- kidney (1)
- la antigens (1)
- pancreas Islets (1)
- pig or swine (1)
- real-time glucose monitoring (1)
- regeneration (1)
- renal transplantation (1)
- small bowel (1)
- streptozotocin (1)
- total pancreatectomy (1)
- transplantation (1)
- xenogeneic transplantation (1)
- xenogenic transplantation (1)
To decrease immunogenicity of the rat kidney, grafts were perfused with an anti-MHC class li monoclonal antibody (mAb ). How effectively this procedure blocked dass li-positive cells, which were mainly dendritic in appearance, was checked by immunostaining renal sections after perfusion and comparing them with in vitro stained sections. Optimum conditions were applied for graft pretreatment before transplantation. This procedure prolonged graft survival, though not satisfactorily from the biological point ofview (9.6 ± 0.8 versus 7.7 ± 0.5 days in the control group; P < 0.02). The dendritic cells were not killed but blocked. Several hours after transplantation, the mAb dissociated from these dass li-positive cells. It was also shown that donor cells migrate into the recipient's spieen early after transplantation. The number of these cells was smaller when the transplanted organ was perfused with the mAb. Further studies are suggested to deplete the graft of donor dendritic cells more adequately. They should also combine graft perfusion with antidass II mAb and recipient immunosuppression at reduced doses.
Abstract: For isolating islets from the porcine pancreas, we established a semiautomated digestion method. Although the isolation technique was standardized and collagenase of controlled quality was used, until now the reproducibility of high islet yields was unsatisfactory. Our hypothesis was that pancreatic trypsin was responsible for this failure. The aim of this study was to investigate the effect of endogenous trypsin on islet yield. Our results demonstrate that a high trypsin level correlates with poor islet yield, whereas low trypsin activity always correlates with high islet yield. Specific inhibition of trypsin results in low trypsin activity and reproducible, high islet yields.
Because successful human islet transplantation requires large quantities of viable islets that must be separated from the highly immunogenic exocrine tissue and because handpicking is too time-consuming and laborious to be clinically relevant, a new approach for solving this problem has been established in rat models. It is based on the principle that magnetic microspheres (MMSs) coupled to lectins with binding specificity for the exocrine tissue portion are trapped in an electromagnetic field, thus providing effluent islets of a high degree of purity. In this study our aim was to adapt this princip'le to human islet preparations. In this context our prime interest was focused on a lectin suitable for human pancreatic tissue. Of 19 different lectins tested, only 1, Wisteria floribunda agglutinin (WFA), is suitable, as shown by immunofluorescence, MMS-Iectin binding, and magnetic separation