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We model Milky Way like isolated disk galaxies in high resolution three-dimensional hydrodynamical simulations with the adaptive mesh refinement code Enzo. The model galaxies include a dark matter halo and a disk of gas and stars. We use a simple implementation of sink particles to measure and follow collapsing gas, and simulate star formation as well as stellar feedback in some cases. We investigate two largely different realizations of star formation. Firstly, we follow the classical approach to transform cold, dense gas into stars with an fixed efficiency. These kind of simulations are known to suffer from an overestimation of star formation and we observe this behavior as well. Secondly, we use our newly developed FEARLESS approach to combine hydrodynamical simulations with a semi-analytic modeling of unresolved turbulence and use this technique to dynamically determine the star formation rate. The subgrid-scale turbulence regulated star formation simulations point towards largely smaller star formation efficiencies and henceforth more realistic overall star formation rates. More work is necessary to extend this method to account for the observed highly supersonic turbulence in molecular clouds and ultimately use the turbulence regulated algorithm to simulate observed star formation relations.
Analytical ultracentrifugation (AUC) is a first principles based method to determine absolute sedimentation coefficients and buoyant molar masses of macromolecules and their complexes, reporting on their size and shape in free solution. The purpose of this multi-laboratory study was to establish the precision and accuracy of basic data dimensions in AUC and validate previously proposed calibration techniques. Three kits of AUC cell assemblies containing radial and temperature calibration tools and a bovine serum albumin (BSA) reference sample were shared among 67 laboratories, generating 129 comprehensive data sets. These allowed for an assessment of many parameters of instrument performance, including accuracy of the reported scan time after the start of centrifugation, the accuracy of the temperature calibration, and the accuracy of the radial magnification. The range of sedimentation coefficients obtained for BSA monomer in different instruments and using different optical systems was from 3.655 S to 4.949 S, with a mean and standard deviation of (4.304\(\pm\)0.188) S (4.4%). After the combined application of correction factors derived from the external calibration references for elapsed time, scan velocity, temperature, and radial magnification, the range of s-values was reduced 7-fold with a mean of 4.325 S and a 6-fold reduced standard deviation of \(\pm\)0.030 S (0.7%). In addition, the large data set provided an opportunity to determine the instrument-to-instrument variation of the absolute radial positions reported in the scan files, the precision of photometric or refractometric signal magnitudes, and the precision of the calculated apparent molar mass of BSA monomer and the fraction of BSA dimers. These results highlight the necessity and effectiveness of independent calibration of basic AUC data dimensions for reliable quantitative studies.