Refine
Has Fulltext
- yes (1)
Is part of the Bibliography
- yes (1) (remove)
Year of publication
- 2022 (1)
Document Type
- Book article / Book chapter (1) (remove)
Language
- English (1)
Keywords
- RNA (1)
- RNA cleavage (1)
- catalytic DNA (1)
- deoxyribozymes (1)
- epitranscriptomics (1)
- in vitro selection (1)
- modified RNA nucleotides (1)
Institute
Schriftenreihe
- Methods in Molecular Biology 2533 (1) (remove)
EU-Project number / Contract (GA) number
- 682586 (1)
Deoxyribozymes are artificially evolved DNA molecules with catalytic abilities. RNA-cleaving deoxyribozymes have been recognized as an efficient tool for detection of modifications in target RNAs and provide an alternative to traditional and modern methods for detection of ribose or nucleobase methylation. However, there are only few examples of DNA enzymes that specifically reveal the presence of a certain type of modification, including N6-methyladenosine, and the knowledge about how DNA enzymes recognize modified RNAs is still extremely limited. Therefore, DNA enzymes cannot be easily engineered for the analysis of desired RNA modifications, but are instead identified by in vitro selection from random DNA libraries using synthetic modified RNA substrates. This protocol describes a general in vitro selection stagtegy to evolve new RNA-cleaving DNA enzymes that can efficiently differentiate modified RNA substrates from their unmodified counterpart.