Refine
Has Fulltext
- yes (72)
Is part of the Bibliography
- yes (72)
Year of publication
Document Type
- Journal article (72) (remove)
Language
- English (72) (remove)
Keywords
- Biochemie (60)
- Neurospora crassa (4)
- bone morphogenetic proteins (2)
- receptor signalling (2)
- ATP carrier (1)
- ATP synthase (1)
- ATPase mutants (1)
- Aminosäuren (1)
- Apoptosis (1)
- B cell receptors (1)
- B cells (1)
- BMP (1)
- BMP antagonist (1)
- Biologie (1)
- Bone morphogenetic protein-2 (1)
- Bone regeneration (1)
- BrdU-Hoechst (1)
- CYR61 (1)
- Cancer (1)
- DNA mediated gene transfer (1)
- Dicyclohexylcarbodiimid (1)
- E. coli atp operon (1)
- Ectopic bone formation (1)
- Escherichia coli (1)
- Escherichia coli-derived recombinant human bone morphogenetic protein-2 (1)
- IL-2 (1)
- IL-4 (1)
- IL-7 (1)
- Interleukin 2 (1)
- Interleukin 4 (1)
- Interleukin 4 (human) (1)
- Interleukin 7 (1)
- Interleukin-4 (1)
- Mechanisms (1)
- Mund-Kiefer-Gesichts-Chirurgie (1)
- Myofibroblast differentiation (1)
- NMR (1)
- NMR spectroscopy (1)
- Oncogene (1)
- Oralchirurgie (1)
- Physiologische Chemie (1)
- Platelet-derived growthfactor (1)
- Polylactide-co-glycolide (1)
- Proliferation (1)
- R. sphaeroidesl (1)
- Receptor (1)
- Recombinant (1)
- Recombinant DNA (1)
- Smooth-muscle-cells (1)
- Structure-function (1)
- T-Lymphozyt (1)
- T-cells (1)
- TGF-beta (1)
- TGF-β superfamily (1)
- Tumor cell (1)
- amino acid sequence (1)
- antagonist (1)
- automated solid-phase Edman degradation (1)
- b/c1 complex (1)
- bacteriophage lambda (1)
- cDNA (1)
- carbenes (1)
- cartilage induction (1)
- cell binding (1)
- cell cycle (1)
- cell proliferation (1)
- cloning (1)
- conduction de protons (1)
- crystal structure (1)
- cytokines (1)
- drug design (1)
- drug design/partial agonists (1)
- electrostatic potential (1)
- expression plasmid (1)
- flow cytometry (1)
- gene (1)
- gene expression (1)
- growth and differentiation factor 5 (1)
- hematopoietic receptors (1)
- human growth factor (1)
- hybrid-selected translation (1)
- in vitro and in vivo expression (1)
- in vitro expression (1)
- inhibition of H+ translocation (1)
- interleukin 2 (1)
- interleukin 4 (1)
- interleukins (1)
- ligand-receptor complex (1)
- ln vitro mutagenesis (1)
- mRNA and gene (1)
- membrane proteins (1)
- membrane receptor signaling (1)
- mitochondrial ADP (1)
- mitochondrially translated (1)
- molecular recognition (1)
- multiple myeloma (1)
- neutralizing antibodies (1)
- nucleotide sequence (1)
- packaging (1)
- partial agonists (1)
- polycistronic mRNA (1)
- proliferation assays (1)
- protein pathway (1)
- protein structure (1)
- protein-protein recognition (1)
- proteines membranaires (1)
- proteolipid (1)
- proton conduction (1)
- screening (1)
- sequence subunit (1)
- signal transduction (1)
- signaling (1)
- subunit stoichiometry (1)
- translational initiation (1)
- von Willebrand type C domain (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (72) (remove)
Sonstige beteiligte Institutionen
Receptor binding properties of four-helix-bundle growth factors deduced from electrostatic analysis
(1994)
Hormones of the hematopoietin class mediate signal transduction by binding to specific transmembrane receptors. Structural data show that the human growth hormone (hGH) forms a complex with a homodimeric receptor and that hGH is a member of a class of hematopoietins possessing an antiparallel 4-a-helix bundle fold. Mutagenesis experiments suggest that electrostatic interactions may have an important influence on hormonereceptor recognition. In order to examine the specificity of hormone-receptor complexation, an analysis was made of the electrostatic potentials of hGH, interleukin-2 (IL-2), interleukin-4 (IL-4), granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), and the hGH and IL-4 receptors. The binding surfaces of hGH and its receptor, and of IL-4 and its receptor, show complementary electrostatic potentials. The potentials of the hGH and its receptor display approximately 2-fold rotational symmetry because the receptor subunits are identical. In contrast, the potentials of GM-CSF and IL-2 Iack such symmetry, consistent with their known high affinity for hetero-oligomeric receptors. Analysis of the electrostatic potentials supports a recently proposed hetero-oligomeric model for a high-affinity IL-4 receptor and suggests a possible new receptor binding mode for G-CSF; it also provides valuable information for guiding structural and mutagenesis studies of signal-transducing proteins and their receptors.
Nucleotide sequence of the cloned mRNA and gene of the ADP/ATP carrier from Neurospora crassa
(1984)
A cDNA complementary to the mRNA of the ADPIATP carrier from Neurospora crassa was identified among ordered cDNA clones by hybridizing total polyadenylated RNA to pools of 96 cDNA recombinant plasmids and subsequent cellfree translation of hybridization-selected mRNA. Further carrier cDNAs were found by colony fdter hybridization at a frequency of 0.2-0.3%. The gene of the carrier was cloned and isolated on a 4.6-kbp EcoRl fragment of total Neurospora DNA, and the start of the mRNA was determined by Sl nuclease mapping. From the nucleotide sequence of the cDNA and the genomic DNA, the primary structure of the gene, of the mRNA and of the ADP I ATP carrier protein could be deduced. The gene occurs in a single copy in the genome and related genes are absent. It contains two short introns, and a pyrimidine-rieb promoter region. The mRNA has a 46-bp 5 1 end and a 219-bp 3 1 end. There is an open reading frame coding for the 313 amino acid residues of the Neurospora carrier protein. The amino acid sequence is homologous in 148 positions with the established primary structure of the beef heart carrier.