Refine
Has Fulltext
- yes (21)
Is part of the Bibliography
- yes (21)
Year of publication
Document Type
- Journal article (21)
Language
- English (21)
Keywords
- Merkel cell carcinoma (8)
- polyomavirus (4)
- cell staining (3)
- melanoma (3)
- Merkel cell polyomavirus (2)
- PD-L1 (2)
- apoptosis (2)
- large T antigen (2)
- melanomas (2)
- polymavirus (2)
- B-cell lymphoma (1)
- B-cells (1)
- BRAF (1)
- CD274 (1)
- CD30 (1)
- CD8(+) (1)
- Cutaneous lymphoma (1)
- EGR1 (1)
- Epigenetic regulation (1)
- GSK3 (1)
- HDAC (1)
- Hautkrebs (1)
- Histone deacetylase (1)
- JAK2 (1)
- Krebs <Medizin> (1)
- LSD1 (1)
- Large T antigen (1)
- Lysine-specific methylase (1)
- MCC (1)
- MCPyV (1)
- MCV-negative (1)
- Medizin (1)
- Melanoma (1)
- PIK3CA mutations (1)
- RNA modification (1)
- T antigen (1)
- T-antigen (1)
- T-antigens (1)
- TP53 (1)
- Tubulin (1)
- YTHDF1 (1)
- akt (1)
- antibody–drug conjugates (1)
- antitumor immunity (1)
- artesunate (1)
- breast cancer (1)
- cancer (1)
- cancer treatment (1)
- case report (1)
- cell cycle (1)
- cell membranes (1)
- chromatin regulator (1)
- copy number variations (1)
- coronavirus disease 2019 (1)
- cultured fibroplasts (1)
- cutaneous T-cell lymphoma and Merkel cell carcinoma (1)
- cutaneous T-cell-lymphoma (1)
- epigenetic reader (1)
- epitranscriptome (1)
- expression (1)
- ferroptosis (1)
- glycogen synthase kinase 3 (1)
- heat shock response (1)
- histology (1)
- histone (1)
- histone methyltransferase PRDM8 (1)
- immune cell infiltration (1)
- immunohistochemistry techniques (1)
- large cell transformation (1)
- lung cancer (1)
- lymphocytes (1)
- m\(^6\)A (1)
- melanin (1)
- melanocytes (1)
- melanoma cell (1)
- melanoma cells (1)
- membrane proteins (1)
- membrans proteins (1)
- miRNA (1)
- microvessel density (1)
- migraine (1)
- mycosis fungoides (1)
- oncodermatology (1)
- p53 (1)
- panel sequencing (1)
- phthalazinone pyrazole (1)
- polymerase chain reaction (1)
- rare (1)
- reporter genes (1)
- responses (1)
- retinoblastoma protein (1)
- senescence (1)
- severe acute respiratory syndrome coronavirus 2 (1)
- skin squamous cell carcinoma (1)
- squamous cell (1)
- subtypes (1)
- survival (1)
- tertiary lymphoid structures (1)
- triptan (1)
- tumor (1)
- tumor suppressor genes (1)
- tumors (1)
- vascular endothelial growth (1)
- vesicles (1)
- viral carcinogenesis (1)
Institute
Merkel cell carcinoma (MCC) is an aggressive skin cancer frequently caused by the Merkel cell polyomavirus (MCPyV), and proliferation of MCPyV-positive MCC tumor cells depends on the expression of a virus-encoded truncated Large T antigen (LT) oncoprotein. Here, we asked in which phases of the cell cycle LT activity is required for MCC cell proliferation. Hence, we generated fusion-proteins of MCPyV-LT and parts of geminin (GMMN) or chromatin licensing and DNA replication factor1 (CDT1). This allowed us to ectopically express an LT, which is degraded either in the G1 or G2 phase of the cell cycle, respectively, in MCC cells with inducible T antigen knockdown. We demonstrate that LT expressed only in G1 is capable of rescuing LT knockdown-induced growth suppression while LT expressed in S and G2/M phases fails to support proliferation of MCC cells. These results suggest that the crucial function of LT, which has been demonstrated to be inactivation of the cellular Retinoblastoma protein 1 (RB1) is only required to initiate S phase entry.