Refine
Has Fulltext
- yes (23)
Is part of the Bibliography
- yes (23)
Year of publication
Document Type
- Journal article (22)
- Doctoral Thesis (1)
Language
- English (23)
Keywords
- Phylogenie (2)
- bee decline (2)
- foraging (2)
- honey bees (2)
- metabarcoding (2)
- next generation sequencing (2)
- solitary bees (2)
- 16S metabarcoding (1)
- 26S RDNA Data (1)
- Algen (1)
- Alps (1)
- AmGr1 (1)
- AmGr2 (1)
- AmGr3 (1)
- American foulbrood (1)
- Angiopoietin-like 4 (1)
- Anticoagulants (1)
- Bacillus (1)
- Barcodes (1)
- Biologie (1)
- Bocas-del-Toro (1)
- Bone disease (1)
- COI (1)
- Coagulation factor IX (1)
- Coexpression (1)
- Colonial volvocales chlorophyta (1)
- Coumarin (1)
- DNA (1)
- DNA barcoding (1)
- DNS-Sequenz (1)
- Dasycladales chlorophyta (1)
- Endothelial growth-factor (1)
- European foulbrood (1)
- Evolution (1)
- Gene expression profiling (1)
- Halictidae (1)
- ITS2 (1)
- Land plants (1)
- Llullaillaco Volcano (1)
- Meliponini (1)
- Mesenchymal stem cells (1)
- Molecular systematics (1)
- Multiple myeloma (1)
- NGS (1)
- Nuclear RDNA (1)
- Orthoptera (1)
- Osmia bicornis (1)
- Osteogenic precursor cells (1)
- Paenibacillus (1)
- Paenibacterin (1)
- Pollen (1)
- Polymerase chain reaction (1)
- Profile distances (1)
- RBCL Gene-sequences (1)
- Ribosomale RNS (1)
- Secondary structure (1)
- Sekundärstruktur (1)
- Sporosarcina (1)
- Subtercola vilae (1)
- VKORC1 (1)
- Vitamin K epoxide reductase (1)
- Warfarin (1)
- Williamsia sp. ARP1 (1)
- adaption (1)
- aerobiology (1)
- allergy (1)
- angiogenic cytokines (1)
- annotation (1)
- anti-microbial activit (1)
- arabidopsis thaliana (1)
- assembly (1)
- bacteria (1)
- bacterial genomics (1)
- bacterial pathogens (1)
- bacterial transmission (1)
- bee disease (1)
- bees (1)
- bioassays (1)
- biodiversity (1)
- biodiversity exploratories (1)
- chemical mimicry (1)
- chrysididae (1)
- cold adaptation (1)
- cuticular chemistry (1)
- cuticular hydrocarbons (1)
- dendrobates pumilio (1)
- diet breadth (1)
- distance‐based specialization index (1)
- diversity (1)
- draft genome (1)
- elevational gradient (1)
- environmental monitoring (1)
- evolutionary arms race (1)
- floral resources (1)
- food safety (1)
- foraging patterns (1)
- functional complementarity (1)
- functional redundancy (1)
- gene-expression (1)
- genome analysis (1)
- gray tree frogs (1)
- gustatory receptors (Grs) (1)
- gut bacteria (1)
- herbivores (1)
- high throughput sequencing (1)
- honeybee taste perception (1)
- hymenoptera (1)
- illumina MiSeq platform (1)
- in-vitro (1)
- interaction networks (1)
- larvae (1)
- male mating success (1)
- marrow stromal cells (1)
- mesenchymal stem-cells (1)
- microbiome (1)
- microbiome metabarcoding (1)
- microclimate (1)
- monoclonial gammopathy (1)
- nest microbiota (1)
- nutrition (1)
- nutritional ecology (1)
- oophaga pumilio (1)
- operational sex ratio (1)
- osmia (1)
- paleoecology (1)
- palynolog (1)
- palynology (1)
- pathogen (1)
- pathogen vector (1)
- peripheral-blood (1)
- philanthidae (1)
- phyllosphere (1)
- phylogenetics (1)
- phylogenomics (1)
- phylogeny evolution (1)
- pied flycatchers (1)
- plant richness (1)
- plant-insect interactions (1)
- plant–insect interactions (1)
- plant–microbe–pollinator triangle (1)
- pollen (1)
- pollen metabarcoding (1)
- pollen nutrients (1)
- pollen provisions (1)
- pollination (1)
- pollination ecology (1)
- pollination network (1)
- pollinator decline (1)
- precedes multiple-myeloma (1)
- proboscis extension response (PER) (1)
- rRNA (1)
- sampling behavior (1)
- season (1)
- secondary invader (1)
- secondary structure (1)
- sequence (1)
- sequential mate choice (1)
- solitary bee (1)
- sugar responsiveness (1)
- systematic affiliation (1)
- temperature gradient (1)
- treefrogs hyla-gratiosa (1)
- undetermined significance (1)
- whole genome sequencing (1)
- wild bees (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (23) (remove)
Sonstige beteiligte Institutionen
ResearcherID
- D-1221-2009 (1)
EU-Project number / Contract (GA) number
- 289706 (1)
Cryptic species and hidden ecological interactions of halictine bees along an elevational gradient
(2021)
Changes of abiotic and biotic conditions along elevational gradients represent serious challenges to organisms which may promote the turnover of species, traits and biotic interaction partners. Here, we used molecular methods to study cuticular hydrocarbon (CHC) profiles, biotic interactions and phylogenetic relationships of halictid bees of the genus Lasioglossum along a 2,900 m elevational gradient at Mt. Kilimanjaro, Tanzania. We detected a strong species turnover of morphologically indistinguishable taxa with phylogenetically clustered cryptic species at high elevations, changes in CHC profiles, pollen resource diversity, and a turnover in the gut and body surface microbiome of bees. At high elevations, increased proportions of saturated compounds in CHC profiles indicate physiological adaptations to prevent desiccation. More specialized diets with higher proportions of low‐quality Asteraceae pollen imply constraints in the availability of food resources. Interactive effects of climatic conditions on gut and surface microbiomes, CHC profiles, and pollen diet suggest complex feedbacks among abiotic conditions, ecological interactions, physiological adaptations, and phylogenetic constraints as drivers of halictid bee communities at Mt. Kilimanjaro.
Honeybees (Apis mellifera) need their fine sense of taste to evaluate nectar and pollen sources. Gustatory receptors (Grs) translate taste signals into electrical responses. In vivo experiments have demonstrated collective responses of the whole Gr-set. We here disentangle the contributions of all three honeybee sugar receptors (AmGr1-3), combining CRISPR/Cas9 mediated genetic knock-out, electrophysiology and behaviour. We show an expanded sugar spectrum of the AmGr1 receptor. Mutants lacking AmGr1 have a reduced response to sucrose and glucose but not to fructose. AmGr2 solely acts as co-receptor of AmGr1 but not of AmGr3, as we show by electrophysiology and using bimolecular fluorescence complementation. Our results show for the first time that AmGr2 is indeed a functional receptor on its own. Intriguingly, AmGr2 mutants still display a wildtype-like sugar taste. AmGr3 is a specific fructose receptor and is not modulated by a co-receptor. Eliminating AmGr3 while preserving AmGr1 and AmGr2 abolishes the perception of fructose but not of sucrose. Our comprehensive study on the functions of AmGr1, AmGr2 and AmGr3 in honeybees is the first to combine investigations on sugar perception at the receptor level and simultaneously in vivo. We show that honeybees rely on two gustatory receptors to sense all relevant sugars.
The composition and richness of herbivore and plant assemblages change along climatic gradients, but knowledge about associated shifts in specialization is scarce and lacks controlling for the abundance and phylogeny of interaction partners. Thus, we aimed to test whether the specialization of phytophagous insects in insect‐plant interaction networks decreases toward cold habitats as predicted by the ‘altitude niche‐breadth hypothesis’ to forecast possible consequences of interaction rewiring under climate change. We used a non‐invasive, standardized metabarcoding approach to reconstruct dietary relationships of Orthoptera species as a major insect herbivore taxon along a broad temperature gradient (~12°C) in Southern Germany. Based on Orthoptera surveys, feeding observations, collection of fecal pellets from >3,000 individuals of 54 species, and parallel vegetation surveys on 41 grassland sites, we quantified plant resource availability and its use by herbivores. Herbivore assemblages were richer in species and individuals at sites with high summer temperatures, while plant richness peaked at intermediate temperatures. Corresponding interaction networks were most specialized in warm habitats. Considering phylogenetic relationships of plant resources, however, the specialization pattern was not linear but peaked at intermediate temperatures, mediated by herbivores feeding on a narrow range of phylogenetically related resources. Our study provides empirical evidence of resource specialization of insect herbivores along a climatic gradient, demonstrating that resource phylogeny, availability, and temperature interactively shape the specialization of herbivore assemblages. Instead of low specialization levels only in cold, harsh habitats, our results suggest increased generalist feeding due to intraspecific changes and compositional differences at both ends of the microclimatic gradient. We conclude that this nonlinear change of phylogeny‐based resource specialization questions predictions derived from the ‘altitude‐niche breadth hypothesis’ and highlights the currently limited understanding of how plant‐herbivore interactions will change under future climatic conditions.