Refine
Has Fulltext
- yes (15)
Is part of the Bibliography
- yes (15)
Year of publication
Document Type
- Journal article (15)
Language
- English (15)
Keywords
- feeding (3)
- Drosophila melanogaster (2)
- circadian rhythms (2)
- drosophila melanogaster (2)
- neurons (2)
- neuropeptides (2)
- CCAP (1)
- Cimex lectularius (1)
- Drosophila (1)
- Hirschsprung disease (1)
- Learning and memory (1)
- M14 carboxypeptidasses (1)
- MALDI imaging (1)
- Neural circuits (1)
- Olig2 (1)
- acylcarnitine (1)
- adult drosophila (1)
- bed bug (1)
- biological locomotion (1)
- blowfly calliphora-vomitoria (1)
- boundary cap (1)
- brain (1)
- bursicon (1)
- cardioactive factor (1)
- cell staining (1)
- central clocks (1)
- central-nervous-system (1)
- chronobiology (1)
- circadian clock (1)
- circadian rhythm (1)
- differentiation (1)
- direct muss spectrometric profiling (1)
- ecdysis (1)
- eclosion (1)
- expression (1)
- factor Sox10 (1)
- feeding behavior (1)
- food consumption (1)
- friut fly behaviour (1)
- fruit fly (1)
- functional characterization (1)
- gene expression (1)
- genome (1)
- hemolymph lipids (1)
- human ectoparasite (1)
- hybridomas (1)
- immune serum (1)
- immunoreactive neurons (1)
- infrared radiation (1)
- intestinal control (1)
- kisspeptin/galanin/spexin signalling (1)
- learning (1)
- library screening (1)
- light pulses (1)
- light-driven metabolism (1)
- lipidomics (1)
- locomotor activity (1)
- mass spectometry (1)
- metabolism and growth (1)
- metabolomics (1)
- mitochondrial activity (1)
- molting (1)
- monoclonal antibodies (1)
- mouse model (1)
- myoinhibitory peptide (1)
- neobellieria bullata (1)
- neuroimaging (1)
- neuromodulation (1)
- neuropeptide pathway (1)
- neuropeptide signalling (1)
- neuropeptidomics (1)
- optogenetics (1)
- paracrine release (1)
- peptidomoics (1)
- peripheral clocks (1)
- peripheral nervous system (1)
- protein processing (1)
- signal peptides (1)
- sleep (1)
- sleep/activity (1)
- social insect (1)
- spinal-cord (1)
- stem cells (1)
- synaptic signaling (1)
- transcriptomics (1)
- tryptophan (1)
- volume transmission (1)
Institute
The fruit fly Drosophila is a prime model in circadian research, but still little is known about its circadian regulation of metabolism. Daily rhythmicity in levels of several metabolites has been found, but knowledge about hydrophobic metabolites is limited. We here compared metabolite levels including lipids between period\(^{01}\) (per\(^{01}\)) clock mutants and Canton-S wildtype (WT\(_{CS}\)) flies in an isogenic and non-isogenic background using LC–MS. In the non-isogenic background, metabo-lites with differing levels comprised essential amino acids, kynurenines, pterinates, glycero(phospho)lipids, and fatty acid esters. Notably, detectable diacylglycerols (DAG) and acylcarnitines (AC), involved in lipid metabolism, showed lower levels in per\(^{01}\) mutants. Most of these differences disappeared in the isogenic background, yet the level differences for AC as well as DAG were consistent for fly bodies. AC levels were dependent on the time of day in WTCS in phase with food consumption under LD conditions, while DAGs showed weak daily oscillations. Two short-chain ACs continued to cycle even in constant darkness. per\(^{01}\) mutants in LD showed no or very weak diel AC oscillations out of phase with feeding activity. The low levels of DAGs and ACs in per\(^{01}\) did not correlate with lower total food consumption, body mass or weight. Clock mutant flies showed higher sensitivity to starvation independent of their background-dependent activity level. Our results suggest that neither feeding, energy storage nor mobilisation is significantly affected in per\(^{01}\) mutants, but point towards impaired mitochondrial activity, supported by upregulation of the mitochondrial stress marker 4EBP in the clock mutants