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Year of publication

  • 2011 (1) (remove)

Keywords

  • antigen processing and recognition (1)
  • drosophila melanogaster (1)
  • gel electrophoresis (1)
  • hybridomas (1)
  • immunohistochemistry techniques (1)
  • immunoprecipitation (1)
  • neuropil (1)
  • silver staining (1)

Author

  • Hofbauer, Alois (1) (remove)

Institute

  • Institut für Klinische Neurobiologie (1)
  • Rudolf-Virchow-Zentrum (1)
  • Theodor-Boveri-Institut für Biowissenschaften (1)

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Identification of Eps15 as Antigen Recognized by the Monoclonal Antibodies aa2 and ab52 of the Wuerzburg Hybridoma Library against Drosophila Brain (2011)
Partho, Halder ; Chen, Yi-chun ; Brauckhoff, Janine ; Hofbauer, Alois ; Dabauvalle, Marie-Christine ; Lewandrowski, Urs ; Winkler, Christiane ; Sickmann, Albert ; Buchner, Erich
The Wuerzburg Hybridoma Library against the Drosophila brain represents a collection of around 200 monoclonal antibodies that bind to specific structures in the Drosophila brain. Here we describe the immunohistochemical staining patterns, the Western blot signals of one- and two-dimensional electrophoretic separation, and the mass spectrometric characterization of the target protein candidates recognized by the monoclonal antibodies aa2 and ab52 from the library. Analysis of a mutant of a candidate gene identified the Drosophila homolog of the Epidermal growth factor receptor Pathway Substrate clone 15 (Eps15) as the antigen for these two antibodies.
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