Förderzeitraum 2020
Refine
Has Fulltext
- yes (216)
Is part of the Bibliography
- yes (216)
Document Type
- Journal article (216)
Language
- English (216)
Keywords
- multiple myeloma (5)
- hypophosphatasia (4)
- relapse (4)
- toxicity (4)
- binding (3)
- brain (3)
- cell death (3)
- chemistry (3)
- expression (3)
- hypoxia (3)
- inflammation (3)
- management (3)
- mortality (3)
- mouse (3)
- pain (3)
- prediction (3)
- stem cells (3)
- technology (3)
- wearable (3)
- Acromyrmex fracticornis (2)
- B cells (2)
- Bone marrow (2)
- Bordetella pertussis (2)
- DNA double-strand breaks (2)
- Expansion microscopy (2)
- Fabry disease (2)
- HGF (2)
- HNSCC (2)
- Lolium perenne (2)
- MPS1 (2)
- MSC (2)
- Met (2)
- Metastatic breast cancer (2)
- SARS-CoV-2 (2)
- Staphylococcus aureus (2)
- amyotrophic lateral sclerosis (2)
- association (2)
- astrocytoma (2)
- autophagy (2)
- cardiorespiratory fitness (2)
- cell cycle (2)
- chondrocytes (2)
- circadian clock (2)
- criteria (2)
- crystal structure (2)
- diagnosis (2)
- evolution (2)
- gene expression (2)
- glioblastoma multiforme (2)
- head and neck cancer (2)
- human (2)
- identification (2)
- immune response (2)
- in vitro (2)
- in vivo (2)
- innovation (2)
- invasion (2)
- leaf-cutting ants (2)
- locomotion (2)
- low-grade glioma (2)
- mHealth (2)
- mRNA (2)
- machine learning (2)
- marine natural products (2)
- molecular docking (2)
- obesity (2)
- quality (2)
- quantification (2)
- recognition (2)
- recurrence (2)
- refractory (2)
- repair (2)
- replication (2)
- self-organization (2)
- sequence (2)
- smartwatch (2)
- stimulation (2)
- stroke (2)
- therapy (2)
- transcriptome (2)
- transplantation (2)
- 18F-FDG PET/CT (1)
- 28 (1)
- 4D-MRI (1)
- 53BP1 (1)
- 6-benzylaminopurine (1)
- 68Ga-PSMA ligand PET/CT (1)
- 8-oxoguanine (1)
- ACPC (1)
- ADAM9 (1)
- ADME analysis (1)
- AKT-signaling (1)
- ALPL (1)
- ASE formula (1)
- ATP-adenosine triphosphate (1)
- Acute lymphocytic leukaemia (1)
- Adaptive cell transfer (1)
- Adenovirus (1)
- Adenylate cyclase toxin (1)
- African agriculture (1)
- Agalsidase beta (1)
- Agro-ecology (1)
- Alzheimers disease (1)
- Amplitude (1)
- Anatomic reattachment (1)
- Apis mellifera (1)
- Arrhenius equation (1)
- Arthrography (1)
- Aspergillus medium (1)
- Attitude (1)
- Autologous hematopoietic stem cell transplantation (1)
- Automated analysis (1)
- Axon degeneration (1)
- BPD (1)
- BRAF (1)
- Blood–brain barrier (1)
- Brownian ratchet (1)
- C-tactile fibers (1)
- C. elegans (1)
- CAR T cell (1)
- CCL3 (1)
- CCL4 (1)
- CCL5 (1)
- CCS (1)
- CD10 (1)
- CD38 (1)
- CIT (1)
- CMV reactivation (1)
- COVID 19 (1)
- CT-angiography (1)
- CX3CL1 (1)
- CXCL13 (1)
- CXCR4 (1)
- CXCR7 (1)
- Cancellous bone (1)
- Candida albicans (1)
- Candida auris (1)
- Cardiac dysfunction| Brain natriuretic peptide (1)
- Cardiomyopathy (1)
- Career choice (1)
- Cartilage (1)
- Cartilage regeneration (1)
- Case report (1)
- Charcot–Marie–Tooth disease type 1A (1)
- Chondrogenesis (1)
- Complex medium (1)
- Computer science (1)
- Concealed Information Test (1)
- Cone-beam computed tomography (1)
- Coronavirus Disease 2019 (1)
- CubeSat (1)
- DNA damage (1)
- Design patterns (1)
- Disaster response (1)
- Distal biceps tendon repair (1)
- Doctor (1)
- Donor lymphocytes (1)
- Drosophila melanogaster (1)
- ECM (1)
- Ecosystem services (1)
- Egypt (1)
- Elbow (1)
- Elbow joint (1)
- Elderly patients (1)
- English version (1)
- Enzyme replacement therapy (1)
- Epichloë (1)
- Epichloë spp. (1)
- F-18-FDG PET (1)
- Fabry-associated pain (1)
- Female patients (1)
- Feminisation (1)
- Fifth Dynasty (1)
- FinO family (1)
- Fourthcorner analysis (1)
- Foxp3 (1)
- Ga-68 (1)
- Gender (1)
- Gene expression vectors (1)
- Gene therapy (1)
- Gram-positive bacteria (1)
- Guillain-Barre-Syndrome (1)
- HFQ (1)
- HMDP hydroxymethylene diphosphonate (1)
- HPLC/UPLC methods (1)
- HPP (1)
- HRV (1)
- Health care service research (1)
- Heart failure (1)
- High-throughput data (1)
- Hip joint (1)
- Holothuria spinifera (1)
- Hospital emergency plan (1)
- Hypertrophy (1)
- ICP27 (1)
- IL-4 (1)
- IL-6 (1)
- Image processing (1)
- ImageJ plugin (1)
- In vitro models (1)
- Infectious complications (1)
- Inoculum production (1)
- Insect pests (1)
- Internet of Things (1)
- IoT (1)
- Ionizing radiation (1)
- Ips typographus (1)
- JCV (1)
- Joint capsule (1)
- KRAS (1)
- Kenyon cells (1)
- Kidney function (1)
- LC-HRESIMS (1)
- LSVT-big therapy (1)
- Landscape ecology (1)
- Legume crops (1)
- Ligamentum capitis femoris (1)
- Lower extremity reconstruction (1)
- MEK/ERK-signaling (1)
- MOR202 (1)
- MR neurography (1)
- MSCs (1)
- MYC (1)
- Macrophages (1)
- Mass critical care (1)
- Medical education (1)
- Medical student (1)
- Merkel cell carcinoma (1)
- Merkel cell density (1)
- Merkel cell polyomavirus (1)
- Merlin (1)
- Mesenchymal stem cell (1)
- Microarray analysis (1)
- Microglia (1)
- Mitochondria (1)
- Multidetector computed tomography (1)
- Multivariate analysis (1)
- Myeloma (1)
- NETs (1)
- NFκB (1)
- NOTCH (1)
- NanoFEEP (1)
- Neisseria meningitidis (1)
- Neurodegeneration (1)
- Neuronal ceroid lipofuscinosis (1)
- Nile Delta (1)
- Non-rigid image registration (1)
- Non-simultaneous bilateral distal biceps tendon rupture (1)
- Open-source tool (1)
- Ordination methods (1)
- Osteoarthritis (1)
- P300 (1)
- PD-L1 (1)
- PEComa (1)
- PET (1)
- PET/CT (1)
- PI stacking (1)
- PLEKHG5 (1)
- PML (1)
- PSMA (1)
- Pain questionnaire (1)
- Parkinson’s disease (1)
- Parkionson's disease (1)
- Patient-centered registry (1)
- Patient’s needs (1)
- Pedicled perforator flap (1)
- Plant growth promotion (1)
- Plant root endophyte (1)
- Plant-insect interactions (1)
- Pointing error (1)
- Pollination (1)
- Polymerase chain reaction (1)
- Pom‐PAD‐Dara (1)
- Prodigy (1)
- Propeller flap (1)
- Proprioception (1)
- RLQ analysis (1)
- RNA-Seq analysis (1)
- RNA-dependent RNA polymerase (1)
- RNA-seq (1)
- Radiation biology (1)
- Radiochemotherapy (1)
- Radiotherapy treatment planning (1)
- Rectal cancer (1)
- Red Sea (1)
- Respiratory induced tumor motion (1)
- SAR safety (1)
- SOX9 (1)
- SPECT (1)
- ST-elevation myocardial infarction (1)
- Salmonella (1)
- Sanger sequencing (1)
- Schwann cells (1)
- Scleractinia (1)
- Seahorse XF (1)
- Serendipita indica (1)
- Sert-deficient mice (1)
- Severe Acute Respiratory Syndrome Coronavirus 2 (1)
- Shotgun method (1)
- Skin (1)
- Small-holder agriculture (1)
- Snf1 (1)
- Stroke (1)
- Sunitinib (1)
- Suprascapular nerve (1)
- Surgery (1)
- Suture anchor (1)
- Synthetic biology (1)
- Systemic sclerosis (1)
- T cell receptor (1)
- T cells (1)
- T lymphocytes (1)
- TNAP (1)
- TNF ligand superfamily (1)
- TNF receptor (TNFR) family (1)
- TRP channel (1)
- TRPA1 (1)
- TTK (1)
- Tdp-43 (1)
- Teichholz formula (1)
- Thalassodendron ciliatum (1)
- Time interval (1)
- Tissue engineering (1)
- Toll-like receptor 4 (TLR4) (1)
- Tomography (1)
- Training (1)
- Transcriptomics (1)
- Tregs (1)
- Triangular fibrocartilage (1)
- Tropical agriculture (1)
- Troponin (1)
- Tyrosine kinase inhibition (1)
- UWE-4 (1)
- VLA-1 (1)
- Vegetable juice (1)
- Virtual sequencing (1)
- Visualization (1)
- Western diet (1)
- Winston‐Lutz test (1)
- Wrist (1)
- X-ray computed (1)
- absorption (1)
- acceptance (1)
- acceptance-based strategy (1)
- access caity preparation (1)
- accuracy (1)
- acetabular bone defect (1)
- acids (1)
- action (1)
- activity rhythm (1)
- acute graft-versus-host disease (1)
- acute heart failure (1)
- acute ischemic stroke (1)
- acute pain (1)
- acute respiratory distress syndrome (1)
- adalimumab (1)
- adaptive conflict (1)
- adenosine diphosphate (1)
- adhesion (1)
- adipose-derived stromal/stem cells (ASCs) (1)
- adrenocortical carcinoma (1)
- aerospace engineering (1)
- affect-regulating massage therapy (1)
- affective touch (1)
- agarose (1)
- aging (1)
- agreement (1)
- airway epithelia (1)
- alignmen (1)
- alkaline phosphatase (1)
- alkaloid detection methods (1)
- alkaloids (1)
- alloreactive T cells (1)
- alpha oscillations (1)
- altitudinal gradient (1)
- ambrosia fungi (1)
- amoxicillin (1)
- ampicillin (1)
- amplicon sequencing (1)
- analgesia (1)
- analytics (1)
- anatomic center of rotation (1)
- androgen deprivation therapy (1)
- angioedema (1)
- angiogenesis (1)
- annotation (1)
- antenna (1)
- anterior approach (1)
- anthropometric measurements (1)
- antibody/autoantibody (1)
- anticoagulation (1)
- antithrombotic therapy (1)
- ants (1)
- anxiety (1)
- apolipoprotein-E (1)
- architecture (1)
- areas (1)
- arithmetic calculations (1)
- arthritis (1)
- artificial intelligence (1)
- asfotase alfa (1)
- assay (1)
- asymmetric implant (1)
- atheriosclerosis (1)
- atomic-force microscopy (1)
- atrial fibrillation (1)
- autoantibodies (1)
- autoimmune encephalitis (1)
- autoimmunity (1)
- autologous stem cell transplantation (1)
- autonomic nervous system (1)
- autotoxicity (1)
- axonal transcriptome (1)
- azole resistance (1)
- bacterial infection (1)
- bark and ambrosia beetles (1)
- bark beetle (1)
- basal ganglia (1)
- base of support (1)
- base pairs (1)
- beam computed tomography (1)
- bee conservation (1)
- beetle (1)
- behavioral plasticity (1)
- behavioural plasticity (1)
- beta-diversity (1)
- binding protein (1)
- bioactivity (1)
- biodiversity (1)
- biological macromolecules (1)
- biology (1)
- biomarker (1)
- biomarker variability (1)
- biomechanic (1)
- biomechanical engineering (1)
- biomimetics (1)
- biopsies (1)
- bioreactor culture (1)
- bird communities (1)
- blazars (1)
- blinks (1)
- blood brain barrier (1)
- blood coagulation factor XIII (1)
- blood flow (1)
- blood stream (1)
- blood-brain barrier (1)
- blood–brain barrier (1)
- body (1)
- body mass index (1)
- body psychotherapy (1)
- body size (1)
- bone (1)
- bone tumour (1)
- brain metabolic alterations (1)
- brain pathology (1)
- brain stem (1)
- brain-computer interface (BCI) (1)
- breast cancer (1)
- bridging (1)
- bronchopulmonary dysplasia (1)
- building behavior (1)
- bypass (1)
- cAMP (1)
- cGMP (1)
- calcaneus (1)
- calcium signaling pathway (1)
- canal calcification (1)
- cancer (1)
- cancer metabolism (1)
- candidate genes (1)
- canine adipose-derived mesenchymal stem cells (cAdMSCs) (1)
- canine cancer cell lines (1)
- canine cancer therapy (1)
- canine soft tissue sarcoma (CSTS) (1)
- capsaicin (1)
- capture (1)
- cardiac magnetic resonance imaging (1)
- cardiogenetics (1)
- cardiomyopathy (1)
- care tempis (1)
- cartilage (1)
- cascade (1)
- caspases (1)
- cell-free therapeutics (1)
- cement (1)
- cerebEND cells (1)
- cerebellar tDCS (1)
- cerebellum (1)
- cerebrosides (1)
- channel transport (1)
- chaperone (1)
- characterization (1)
- chemokine receptor (1)
- childhood and adolescence (1)
- chondrogenic differentiation (1)
- chondroprogenitors (1)
- chronic heart failure (1)
- ciliostasis (1)
- classification (1)
- click chemistry (1)
- climate change (1)
- clinical diagnosis (1)
- clinical study (1)
- clinical trial (1)
- clonal hematopoiesis of indeterminate potential (1)
- co-culture (1)
- cognitive impairment (1)
- cognitive strategies (1)
- cold storage (1)
- collective building (1)
- collective pattern (1)
- combination of physical vapor deposition and electrochemical etching (1)
- combinatorial drug predictions (1)
- common garden experiment (1)
- common variable immunodeficiency (1)
- community data (1)
- comparability (1)
- complex behavior (1)
- complexes (1)
- complexity (1)
- complications (1)
- compression syndrome (1)
- concealed identity information (1)
- conditional mutants (1)
- conformational auto-epitope (1)
- connectome (1)
- consciousness (1)
- conservation (1)
- consolidation (1)
- continuous-time SLAM (1)
- contrast agent (1)
- cool-season grass species (1)
- cooperative breeding (1)
- corticosteroids (1)
- coupling-constant dependence (1)
- course (1)
- covalent organic frameworks (1)
- craniosynostosis (1)
- creatine kinase (1)
- cristae (1)
- cross-link repair (1)
- crowdsensing (1)
- crystals (1)
- cutaneous PEComa (1)
- cutaneous innervation (1)
- cybersickness (1)
- cycle (1)
- cyclic adenosine monophosphate (1)
- cyclic nucleotides (1)
- cyclic peptides/cyclopeptides (1)
- cyclopeptide therapy (1)
- cytokines (1)
- cytokinins (1)
- cytome biomarkers (1)
- cytotoxic activity (1)
- cytotoxicity (1)
- daratumumab (1)
- data visualization (1)
- database (1)
- decision support techniques (1)
- decision-making (1)
- defects (1)
- defined humanized test system (1)
- definition (1)
- deformation-based method (1)
- dementia (1)
- dendritic specializations (1)
- dentin dysplasia (1)
- depression (1)
- design (1)
- detection rate (1)
- detoxification (1)
- detrended fluctuation analysis (1)
- development (1)
- developmental biology (1)
- diachronicity (1)
- diapause (1)
- dictator game (1)
- diet (1)
- differentiated thyroid carcinoma (1)
- differentiation (1)
- differentiation capacity (1)
- digital health (1)
- diketopyrrolopyrroles (1)
- discharge definition (1)
- disease (1)
- dispersal ability (1)
- divergence times (1)
- diversity (1)
- docking studies (1)
- domain (1)
- dopamine (1)
- dosimetry (1)
- drosophila (1)
- drug adverse reaction (1)
- drug allergy (1)
- drug hypersensitivity (1)
- drug transporter (1)
- drugs (1)
- dual function (1)
- dual-energy (1)
- duplicate genes (1)
- e-learning (1)
- eHealth (1)
- echocardiography (1)
- ecological momentary assessment (1)
- ecological network (1)
- effects (1)
- efflux pumps (1)
- electric propulsion (1)
- electron (1)
- electron tomography (1)
- element (1)
- embolism/thrombosis (1)
- emotion regulation (1)
- endocytosis (1)
- endophyte (1)
- endurance (1)
- endurance exercise (1)
- endurance training (1)
- energy (1)
- entropy production (1)
- environmental filtering (1)
- enzyme replacement therapy (1)
- epidermis (1)
- epithelial cells (1)
- ergosterol derivative (1)
- essential genes (1)
- establishment (1)
- ethanol (1)
- event-related-potential (ERP) (1)
- evolutionary (1)
- exercise intensity (1)
- exit (1)
- exosomes (1)
- exotic plants (1)
- experimental evaluation (1)
- extracellular vesicle (1)
- extracellular vesicles (1)
- factor-VIII (1)
- facultatively intracellular pathogens (1)
- fear (1)
- fibromyalgia syndrome (1)
- fire (1)
- fixation (1)
- flagellar pocket (1)
- flippase (1)
- flow patterns (1)
- fluconazole resistance (1)
- fluorescence (1)
- fluorescence microscopy (1)
- folliculin (1)
- forest (1)
- forest physiognomy (1)
- forms (1)
- fracture (1)
- free energy (1)
- functional traits (1)
- fungi (1)
- fungus community (1)
- fungus-farming (1)
- fused in sarcoma (1)
- gait initiation (1)
- gametogenesis (1)
- gamma H2AX-foci (1)
- gastric bypass (1)
- gene (1)
- gene therapy (1)
- generalization (1)
- generation (1)
- glacier–permafrost interaction (1)
- glucose starvation (1)
- glucose tolerance (1)
- glycine receptor (1)
- gonococcal invasion (1)
- gradients (1)
- granular (1)
- graph adaptivity (1)
- graph ergonomics (1)
- grass endophytes (1)
- grasslands (1)
- growth (1)
- growth differentiation factor 15 (1)
- guided endodontics (1)
- guidelines (1)
- hMSCs (1)
- habits (1)
- healing and remodelling processes (1)
- heat shock response (1)
- helicase (1)
- hemodialysis (1)
- heterosis (1)
- high contrast (1)
- high-field MRI (1)
- high-risk Prostate Cancer (1)
- hip joint (1)
- homing (1)
- honey bee (1)
- horses (1)
- hospitals (1)
- host (1)
- human neurons (1)
- human respiratory epithelial cells (1)
- hybrid materials (1)
- hydrogels (1)
- hyphae (1)
- hypovitaminosis D (1)
- hypthesis (1)
- iMP (1)
- images (1)
- immune therapy (1)
- immunity (1)
- immunofuorescence double staining (1)
- immunohistochemistry (1)
- immunotherapeutics (1)
- immunotherapies (1)
- immunotherapy (1)
- impact (1)
- implant positioning (1)
- in situ hybridization (1)
- in-orbit experiments (1)
- induced neural stem cells (1)
- infarction volume (1)
- infected-cell protein (1)
- infection rates (1)
- infectious disease (1)
- inflammation-induced tissue demage (1)
- inflammatory demyelinating polyradiculoneuropathy (1)
- inflammatory response (1)
- inhibitory signaling (1)
- insect agriculture (1)
- insects (1)
- integrin (1)
- intensity distribution (1)
- intensity zones (1)
- interaction map (1)
- interactome (1)
- interacts (1)
- internal load (1)
- interoception (1)
- interparticle interaction (1)
- interspecies comparison (1)
- intraepidermal nerve fibre density (1)
- invasiveness (1)
- iodine-131 (1)
- ion channel (1)
- iron oxide nanoparticles (1)
- ischemia (1)
- ischemic stroke (1)
- isocenter (1)
- isosteviol sodium (1)
- isothiocyanates (1)
- jitter (1)
- kidney ischemia/reperfusion injury (1)
- kinease (1)
- kinetics (1)
- kink instability (1)
- knee axis (1)
- knee osteoarthritis (1)
- knock down (1)
- lactate dehydrogenase (1)
- large T antigen (1)
- latency (1)
- left ventricular ejection fraction (1)
- left ventricular hypertrophy (1)
- left ventricular mass index (1)
- lesions (1)
- life (1)
- lifestyle (1)
- ligands (1)
- lipid asymmetry (1)
- liquid chromatography electrospray ionization tandem mass spectrometry (1)
- liraglutide (1)
- literature search (1)
- livestock (1)
- locomotor adaptation (1)
- long-term complications (1)
- low fidelity (1)
- low molecular heparin (1)
- lymphocytes (1)
- m-Health (1)
- mRNA expression (1)
- magnetic resoncance (1)
- malignancy (1)
- mapping (1)
- marine bacteria (1)
- marine fungi (1)
- marine metagenomics (1)
- marine organisms (1)
- massage therapy (1)
- material composition (1)
- mechanical engineering (1)
- mechanical ventilation (1)
- mechanics (1)
- mechanism (1)
- mechanisms (1)
- medaka (1)
- medical education (1)
- meiosis (1)
- melanoma (1)
- membrane occupation (1)
- memory B cells (1)
- menstrual cycles (1)
- messenger RNA (1)
- metabolic adaptation (1)
- metabolic analysis (1)
- metabolic tumour volume (MTV) (1)
- metabolism (1)
- methyltransferase (1)
- mice (1)
- microRNA-221 (1)
- microenvironment (1)
- microglomeruli (1)
- microstructure (1)
- mimetic peptide (1)
- mineralization (1)
- mineraliztion (1)
- mismatches (1)
- mitochondria (1)
- mitochondrial dynamics (1)
- mobile EEG (1)
- mobile operating system differences (1)
- mobile phone (1)
- model (1)
- modular tumor tissue models (1)
- molecular cloning (1)
- molecular structure (1)
- monitoring (1)
- mononuclear (1)
- moon (1)
- moral balancing (1)
- morbidity (1)
- motor entrainment (1)
- mountain permafrost (1)
- mouse models (1)
- movement (1)
- multifocal growth (1)
- multiple sclerosis (1)
- murine (1)
- muscarinic M1 receptor (1)
- musculoskeletal diseases (1)
- mushroom body (1)
- musk myasthenia gravis (1)
- mustard oil bomb (1)
- mutualism (1)
- mutualistic interactions (1)
- mycotoxins (1)
- myelin (1)
- myeloid-derived suppressor cells (MDSCs) (1)
- nanoscale imaging (1)
- nanotopographical surfaces (1)
- natriuretic peptide (1)
- natural disturbance (1)
- natural killer cell (1)
- natural transformation (1)
- near surface geophysics (1)
- neonate (1)
- nerve-fibers (1)
- nervous system (1)
- network (1)
- network specialization index (H2′) (1)
- neural architecture (1)
- neural networks (1)
- neurofascin (1)
- neuromuscular junction (1)
- neuronal network (1)
- neuropathy (1)
- neuroprotection (1)
- neurotransmission (1)
- neutral processes (1)
- neutral sphingomyelinase-2 (1)
- neutrophils (1)
- nicotinamide (1)
- nociception (1)
- nodal knots (1)
- nomes (1)
- non-equilibrium thermodynamics (1)
- non-oncological (1)
- nonspecific alkaline-phosphae (1)
- non‐native plants (1)
- nuclear medicine (1)
- nuclear receptors (1)
- nucleation-elongation (1)
- nucleotide excision repair (1)
- oak wood (1)
- objective structured clinical examination (1)
- obliteration (1)
- olfaction (1)
- ollimator (1)
- oncolytic virus (1)
- ontactin 1 (1)
- opioid (1)
- optogenetics (1)
- oral anticoagulants (1)
- osteoarthritis (1)
- osteocyte network (1)
- osteomalacia (1)
- osteoporosis (1)
- outcome (1)
- outcomes (1)
- outgrowth endothelial cells (1)
- ow-cost photometer (1)
- oxidative phosphorylation (1)
- oxidised lipids (1)
- oxidized phospholipids (1)
- oxygen/glucose deprivation (1)
- package (1)
- pain ratings (1)
- pain regulation (1)
- pain therapy (1)
- paleogeography (1)
- pancreatectomy (1)
- passes (1)
- pathogenesis (1)
- pathophysiology (1)
- patient participation (1)
- patient-specific knee arthroplasty (1)
- pattern (1)
- penicillin allergy (1)
- penicillin hypersensitivity (1)
- peptide tyrosine tyrosine (PYY) (1)
- peptide tyrosine tyrosine 3-36 (PYY3-36) (1)
- perennial ryegrass (1)
- periperal nerve (1)
- peripheral-blood lymphocytes (1)
- periprosthetic infection (1)
- perivascular epitheloid cell tumour (1)
- permanent (1)
- personality (1)
- perylene bismide dye (1)
- phagocytosis (1)
- phase-contrast MRI (1)
- phenology (1)
- phenotype (1)
- phosphatidylethanolamine (1)
- phosphatidylserine (1)
- phosphatidylserine transport (1)
- phosphodiesterases (PDEs) (1)
- phylogenetic trees (1)
- physical activity (1)
- physical performance (1)
- pilot project (1)
- plant composition (1)
- plant fresh/dry weight (1)
- plant–bee visitation networks (1)
- platelet (1)
- platelet physiology (1)
- point of care testing (1)
- polarized cell culture (1)
- polarized epithelium (1)
- polarized training (1)
- pollination (1)
- pollinator friendly plants (1)
- polygenic risk score (1)
- polymerization (1)
- polyphenols (1)
- porphyrins (1)
- post-reconstruction filtering (1)
- post-surgery recovery (1)
- post-transcriptional regulation (1)
- postoperative bleeding (1)
- power consumption (1)
- precision training (1)
- preterm infant (1)
- primary cell culture (1)
- primary immunodeficiencies (1)
- prognosis (1)
- progression (1)
- projection neurons (1)
- proliferation (1)
- promotes (1)
- prosocial behavior (1)
- prostate cancer (1)
- prostate-specific membrane antigen (PSMA) (1)
- protein (1)
- protein kinases (1)
- proteome (1)
- protocadherin gamma C3 (1)
- psychoactive massage (1)
- psychological modulation of pain (1)
- pulmonary arteries (1)
- pulp canal calcification (1)
- pupil size (1)
- purinergic receptors (1)
- quality assurance (1)
- quantitative stigmergy (1)
- question prompt list (1)
- radiation (1)
- radiogenomics (1)
- radioiodine therapy (1)
- rare bone disease (1)
- rates (1)
- reactive electrophilic species (1)
- reactive oxygen species (1)
- real time PCR (1)
- real-world evidence (1)
- recent origin (1)
- receptor cluster (1)
- recognition nexus domain (1)
- recommendation engine (1)
- recovery (1)
- recurrent prostate cancer (1)
- redox homeostasis (1)
- reerse shoulder arthoplasty (1)
- regenerative medicine (1)
- regional species pool (1)
- regulator (1)
- regulatory T cells (1)
- reinnervation (1)
- release (1)
- reliability (1)
- reproducible outcome measure (1)
- resistance (1)
- responses (1)
- responsibility (1)
- retinoic acid (1)
- reveals (1)
- revision arthroplasty (1)
- rheumatoid arhritis (1)
- rickets (1)
- risk (1)
- robustness (1)
- root canal treatment (1)
- rygb (1)
- sRNA atlas (1)
- saccades (1)
- sanders (1)
- sandfish (1)
- scaffolds (1)
- scanner (1)
- schizophrenia (1)
- scoping review (1)
- screw (1)
- seagrass (1)
- second primary malignancy (1)
- secondary site infection (1)
- secretion (1)
- selection (1)
- self-adaptive systems (1)
- self-regulation (1)
- semiconductors (1)
- serotonin receptors (1)
- sex (1)
- sex determination (1)
- shape (1)
- shape-truncation functions (1)
- shear viscosity (1)
- shocks (1)
- shoulder arthroplasty (1)
- shoulder infection (1)
- shoulder neurolysis (1)
- signaling (1)
- simulator sickness (1)
- skeletal (1)
- skin punch biopsy (1)
- slip (1)
- small animal SPECT (1)
- small-animal imaging (1)
- smoking (1)
- smoking cessation (1)
- soccer (1)
- social (1)
- social behavior (1)
- social dominance (1)
- social influence (1)
- social recognition (1)
- social status (1)
- sociality (1)
- software (1)
- software engineering (1)
- solitary bee (1)
- solubility (1)
- solution scattering (1)
- spacer (1)
- spastic (1)
- specialization (1)
- species turnover (1)
- sphingolipid expansion microscopy (1)
- sphingolipids (1)
- sphingosine (1)
- sphingosine kinases (1)
- spleen (1)
- split-belt treadmill (1)
- sporidia (1)
- squamous cell carcinoma (1)
- startle reaction (1)
- state space (1)
- statistical mechanics (1)
- stem cell transplantation (1)
- stepped impedance resonators (1)
- stereotactic radiotherapy (1)
- strategy (1)
- structural basis (1)
- structural synaptic plasticity (1)
- structured illumination microscope (1)
- substance-P (1)
- subunit (1)
- sulforaphane (1)
- sulfur (1)
- superconductivity (1)
- superior (1)
- superparamagnetism (1)
- suprascapular notch (1)
- surgical care (1)
- survival (1)
- switch (1)
- symbiont selection (1)
- symbiosis (1)
- synapse (1)
- synaptic vesicles (1)
- t-lymphocytes (1)
- tactile (1)
- tactually evoked potentials (1)
- target (1)
- targeted gene panel (1)
- targeting (1)
- teeth (1)
- telescope (1)
- temperature dependence of reaction rate (1)
- template (1)
- therapeutic antibody (1)
- thermodynamics (1)
- theta (1)
- thin sections (1)
- thrombemboli (1)
- thromboembolism (1)
- thrombolysis (1)
- thrust direction (1)
- thrust moraine (1)
- thymine (1)
- tight junction (1)
- tight junctions (1)
- time (1)
- time calibration (1)
- tinnitus (1)
- tissue engineering (1)
- tissue model (1)
- tocilizumab (IL-6 inhibitor) (1)
- tool use (1)
- topolectrical circuits (1)
- total knee replacement (1)
- total lesion PSMA (1)
- tracheal cytotoxin (1)
- tranexamic acid (1)
- transactivation (1)
- transceiver array (1)
- transcription factor (1)
- transcription factors (1)
- transcription start sites (1)
- transcriptional regulation (1)
- translation (1)
- translocation (1)
- transmission electron microscopy (1)
- transport (1)
- treatment (1)
- trophic factors (1)
- trophic position (1)
- tropocoronands (1)
- trypanosoma brucei (1)
- tumor microenvironment (1)
- tumor necrosis factor-α (1)
- tumor-infiltrating (1)
- tumour malignancy (1)
- two stage (1)
- type 1 (1)
- ultrastructure (1)
- unreflective actions (1)
- uper-resolution array tomography (1)
- urolithins (1)
- urticaria (1)
- user model (1)
- vaccinia virus (1)
- van‘t Hoff rule (1)
- variability (1)
- vascular (1)
- vertebrate (1)
- vertebrobasilar insufficiency (1)
- vertigo (1)
- vestibular schwannoma (1)
- viral load (1)
- virtual reality (1)
- virulence (1)
- vision (1)
- vitamin D (1)
- vitamin D deficiency (1)
- walking phase (1)
- warfarin interruption (1)
- wheelchair control (1)
- whole exome sequencing (1)
- wild bees (1)
- windthrow (1)
- xenotransplantation (1)
- young females (1)
- youth soccer (1)
- zebrafish (1)
- zonal (1)
- zooxanthellae (1)
- β-diversity (1)
- β1-adrenoceptor/β1-adrenergic receptor (1)
- γ-H2AX (1)
- ∆Np63 (1)
Institute
- Theodor-Boveri-Institut für Biowissenschaften (51)
- Medizinische Klinik und Poliklinik II (23)
- Lehrstuhl für Orthopädie (17)
- Neurologische Klinik und Poliklinik (17)
- Institut für diagnostische und interventionelle Radiologie (Institut für Röntgendiagnostik) (13)
- Medizinische Klinik und Poliklinik I (13)
- Deutsches Zentrum für Herzinsuffizienz (DZHI) (12)
- Klinik und Poliklinik für Anästhesiologie (ab 2004) (12)
- Klinik und Poliklinik für Nuklearmedizin (12)
- Lehrstuhl für Tissue Engineering und Regenerative Medizin (10)
Sonstige beteiligte Institutionen
We report on a currently 76-year-old female patient with relapsed/refractory (RR) multiple myeloma (MM) treated at our institution. This patient had received six lines of therapy including tandem autologous stem cell transplant, proteasome inhibitor, immunomodulatory drugs and CD38 antibody MOR202. At the last relapse, she progressed during treatment with pomalidomide and MOR202. In an individualized therapy concept, we started a multi-agent salvage therapy with pomalidomide, bortezomib, doxorubicin, dexamethasone, and CD38 antibody daratumumab (“Pom-PAD-Dara”), which resulted in a stringent complete remission with minimal residual disease (MRD) negativity after nine cycles. So far, our patient shows a progression free survival of more than 12 months. Our case demonstrates the feasibility of successful CD38 antibody retreatment in a patient with heavily pretreated CD38 antibody resistant MM.
Head and neck squamous cell carcinoma (HNSCC) is a widespread disease with a low survival rate and a high risk of recurrence. Nowadays, immune checkpoint inhibitor (ICI) treatment is approved for HNSCC as a first-line treatment in recurrent and metastatic disease. ICI treatment yields a clear survival benefit, but overall response rates are still unsatisfactory. As shown in different cancer models, hepatocyte growth factor/mesenchymal–epithelial transition (HGF/Met) signaling contributes to an immunosuppressive microenvironment. Therefore, we investigated the relationship between HGF and programmed cell death protein 1 (PD-L1) expression in HNSCC cell lines. The preclinical data show a robust PD-L1 induction upon HGF stimulation. Further analysis revealed that the HGF-mediated upregulation of PD-L1 is MAP kinase-dependent. We then hypothesized that serum levels of HGF and soluble programmed cell death protein 1 (sPD-L1) could be potential markers of ICI treatment failure. Thus, we determined serum levels of these proteins in 20 HNSCC patients before ICI treatment and correlated them with treatment outcomes. Importantly, the clinical data showed a positive correlation of both serum proteins (HGF and sPD-L1) in HNSCC patient’s sera. Moreover, the serum concentration of sPD-L1 was significantly higher in ICI non-responsive patients. Our findings indicate a potential role for sPD-L1 as a prognostic marker for ICI treatment in HNSCC.
The second messengers, cyclic adenosine 3′-5′-monophosphate (cAMP) and cyclic guanosine 3′-5′-monophosphate (cGMP), play important roles in many animal cells by regulating intracellular signaling pathways and modulating cell physiology. Environmental cues like temperature, light, and chemical compounds can stimulate cell surface receptors and trigger the generation of second messengers and the following regulations. The spread of cAMP and cGMP is further shaped by cyclic nucleotide phosphodiesterases (PDEs) for orchestration of intracellular microdomain signaling. However, localized intracellular cAMP and cGMP signaling requires further investigation. Optogenetic manipulation of cAMP and cGMP offers new opportunities for spatio-temporally precise study of their signaling mechanism. Light-gated nucleotide cyclases are well developed and applied for cAMP/cGMP manipulation. Recently discovered rhodopsin phosphodiesterase genes from protists established a new and direct biological connection between light and PDEs. Light-regulated PDEs are under development, and of demand to complete the toolkit for cAMP/cGMP manipulation. In this review, we summarize the state of the art, pros and cons of artificial and natural light-regulated PDEs, and discuss potential new strategies of developing light-gated PDEs for optogenetic manipulation.
Ischemic stroke caused by thromboembolic occlusion of large cerebral arteries, such as the internal carotid (ICA) and/or the middle cerebral artery (MCA), is treated by mechanical thrombectomy (MT). MT allows salvage of the vessel-occluding thrombemboli, which most frequently originate from the left atrium or the left ventricle of the heart or from sites of plaque rupture within large arteries above the heart. Clot composition may influence the efficacy of (intravenous) thrombolysis and MT, respectively. We analyzed 37 human thrombemboli obtained from acute ischemic stroke patients during MT with special emphasis on histological staining of neutrophils and neutrophil extracellular traps (NETs). We found neutrophils as the main cellular component of cerebral thrombemboli but encountered considerable morphological heterogeneity. Neutrophils accumulated in the border region of fibrin-rich structures indicating possible interaction of neutrophils with distinct structural thrombembolus components. Web-like NETs were found in 35 of 37 thrombemboli in varying amounts. NETs were almost exclusively found within fibrin-rich areas. Importantly, stroke etiology, age and present oral anticoagulation was associated with morphological patterns and the amount of neutrophils. Correlation of histological data and imaging data revealed that relative Hounsfield units of cerebral thrombemboli positively correlated with the amount of red blood cells. In summary, our results demonstrate that neutrophils and NETs are substantial constituents of cerebral thrombemboli and contribute to their structural complexity.
Identification of articular cartilage progenitor cells (ACPCs) has opened up new opportunities for cartilage repair. These cells may be used as alternatives for or in combination with mesenchymal stromal cells (MSCs) in cartilage engineering. However, their potential needs to be further investigated, since only a few studies have compared ACPCs and MSCs when cultured in hydrogels. Therefore, in this study, we compared chondrogenic differentiation of equine ACPCs and MSCs in agarose constructs as monocultures and as zonally layered co-cultures under both normoxic and hypoxic conditions. ACPCs and MSCs exhibited distinctly differential production of the cartilaginous extracellular matrix (ECM). For ACPC constructs, markedly higher glycosaminoglycan (GAG) contents were determined by histological and quantitative biochemical evaluation, both in normoxia and hypoxia. Differential GAG production was also reflected in layered co-culture constructs. For both cell types, similar staining for type II collagen was detected. However, distinctly weaker staining for undesired type I collagen was observed in the ACPC constructs. For ACPCs, only very low alkaline phosphatase (ALP) activity, a marker of terminal differentiation, was determined, in stark contrast to what was found for MSCs. This study underscores the potential of ACPCs as a promising cell source for cartilage engineering.
We here present the case of a 67-year-old woman with a history of a slowly progressive, polypous nodule on her left wrist. The lesion was excised, and the histological analysis revealed a clear cell tumour that was relatively sharply demarked from the surrounding tissue extending into the subcutaneous tissue. The tumour showed a characteristic trabecular pattern in which the tumour cells were arranged around numerous vessels. The neoplastic cells had a predominantly epithelioid shape, granular eosinophilic to clear cytoplasm and prominent centrally located nucleoli. The histological differential diagnosis included a metastatic clear-cell renal cell carcinoma and a primary cutaneous perivascular epithelioid cell tumour (PEComa). Immunohistochemically, the tumour cells revealed homogenous expression of HMB-45, MiTF and CD10, whereas MART-1 and S100 were negative. Antibodies against actin marked the trabecularly arranged vessels, and the neoplastic cells yielded a patchy positivity against actin and desmin. Additional immunohistochemical stains against pan-cytokeratin, CAIX, PAX-8 and EMA were negative. Based on the morphologic and immunophenotypic findings, the histological diagnosis of a CD10-positive cutaneous PEComa was made.
Background
Presence of clonal hematopoiesis of indeterminate potential (CHIP) is associated with a higher risk of atherosclerotic cardiovascular disease, cancer, and mortality. The relationship between a healthy lifestyle and CHIP is unknown.
Methods and Results
This analysis included 8709 postmenopausal women (mean age, 66.5 years) enrolled in the WHI (Women's Health Initiative), free of cancer or cardiovascular disease, with deep‐coverage whole genome sequencing data available. Information on lifestyle factors (body mass index, smoking, physical activity, and diet quality) was obtained, and a healthy lifestyle score was created on the basis of healthy criteria met (0 point [least healthy] to 4 points [most healthy]). CHIP was derived on the basis of a prespecified list of leukemogenic driver mutations. The prevalence of CHIP was 8.6%. A higher healthy lifestyle score was not associated with CHIP (multivariable‐adjusted odds ratio [OR] [95% CI], 0.99 [0.80–1.23] and 1.13 [0.93–1.37]) for the upper (3 or 4 points) and middle category (2 points), respectively, versus referent (0 or 1 point). Across score components, a normal and overweight body mass index compared with obese was significantly associated with a lower odds for CHIP (OR, 0.71 [95% CI, 0.57–0.88] and 0.83 [95% CI, 0.68–1.01], respectively; P‐trend 0.0015). Having never smoked compared with being a current smoker tended to be associated with lower odds for CHIP.
Conclusions
A healthy lifestyle, based on a composite score, was not related to CHIP among postmenopausal women. However, across individual lifestyle factors, having a normal body mass index was strongly associated with a lower prevalence of CHIP. These findings support the idea that certain healthy lifestyle factors are associated with a lower frequency of CHIP.
Latency is a key characteristic inherent to any computer system. Motion-to-Photon (MTP) latency describes the time between the movement of a tracked object and its corresponding movement rendered and depicted by computer-generated images on a graphical output screen. High MTP latency can cause a loss of performance in interactive graphics applications and, even worse, can provoke cybersickness in Virtual Reality (VR) applications. Here, cybersickness can degrade VR experiences or may render the experiences completely unusable. It can confound research findings of an otherwise sound experiment. Latency as a contributing factor to cybersickness needs to be properly understood. Its effects need to be analyzed, its sources need to be identified, good measurement methods need to be developed, and proper counter measures need to be developed in order to reduce potentially harmful impacts of latency on the usability and safety of VR systems. Research shows that latency can exhibit intricate timing patterns with various spiking and periodic behavior. These timing behaviors may vary, yet most are found to provoke cybersickness. Overall, latency can differ drastically between different systems interfering with generalization of measurement results. This review article describes the causes and effects of latency with regard to cybersickness. We report on different existing approaches to measure and report latency. Hence, the article provides readers with the knowledge to understand and report latency for their own applications, evaluations, and experiments. It should also help to measure, identify, and finally control and counteract latency and hence gain confidence into the soundness of empirical data collected by VR exposures. Low latency increases the usability and safety of VR systems.
The electric propulsion system NanoFEEP was integrated and tested in orbit on the UWE-4 satellite, which marks the first successful demonstration of an electric propulsion system on board a 1U CubeSat. In-orbit characterization measurements of the heating process of the propellant and the power consumption of the propulsion system at different thrust levels are presented. Furthermore, an analysis of the thrust vector direction based on its effect on the attitude of the spacecraft is described. The employed heater liquefies the propellant for a duration of 30 min per orbit and consumes 103 ± 4 mW. During this time, the respective thruster can be activated. The propulsion system including one thruster head, its corresponding heater, the neutralizer and the digital components of the power processing unit consume 8.5 ± 0.1 mW ⋅μ A\(^{−1}\) + 184 ± 8.5 mW and scales with the emitter current. The estimated thrust directions of two thruster heads are at angles of 15.7 ± 7.6∘ and 13.2 ± 5.5∘ relative to their mounting direction in the CubeSat structure. In light of the very limited power on a 1U CubeSat, the NanoFEEP propulsion system renders a very viable option. The heater of subsequent NanoFEEP thrusters was already improved, such that the system can be activated during the whole orbit period.
Aim: Despite increasing interest in β-diversity, that is the spatial and temporal turnover of species, the mechanisms underlying species turnover at different spatial scales are not fully understood, although they likely differ among different functional groups. We investigated the relative importance of dispersal limitations and the environmental filtering caused by vegetation for local, multi-taxa forest communities differing in their dispersal ability, trophic position and body size.
Location: Temperate forests in five regions across Germany.
Methods: In the inter-region analysis, the independent and shared effects of the regional spatial structure (regional species pool), landscape spatial structure (dispersal limitation) and environmental factors on species turnover were quantified with a 1-ha grain across 11 functional groups in up to 495 plots by variation partitioning. In the intra-region analysis, the relative importance of three environmental factors related to vegetation (herb and tree layer composition and forest physiognomy) and spatial structure for species turnover was determined.
Results: In the inter-region analysis, over half of the explained variation in community composition (23% of the total explained 35%) was explained by the shared effects of several factors, indicative of spatially structured environmental filtering. Among the independent effects, environmental factors were the strongest on average over 11 groups, but the importance of landscape spatial structure increased for less dispersive functional groups. In the intra-region analysis, the independent effect of plant species composition had a stronger influence on species turnover than forest physiognomy, but the relative importance of the latter increased with increasing trophic position and body size.
Main conclusions: Our study revealed that the mechanisms structuring assemblage composition are associated with the traits of functional groups. Hence, conservation frameworks targeting biodiversity of multiple groups should cover both environmental and biogeographical gradients. Within regions, forest management can enhance β-diversity particularly by diversifying tree species composition and forest physiognomy.
Inhibition of the protein kinase MPS1, a mitotic spindle-checkpoint regulator, reinforces the effects of multiple therapies against glioblastoma multiforme (GBM) in experimental settings. We analyzed MPS1 mRNA-expression in gliomas WHO grade II, III and in clinical subgroups of GBM. Data were obtained by qPCR analysis of tumor and healthy brain specimens and correlated with the patients’ clinical data. MPS1 was overexpressed in all gliomas on an mRNA level (ANOVA, p < 0.01) and correlated with tumor aggressiveness. We explain previously published conflicting results on survival: high MPS1 was associated with poorer long term survival when all gliomas were analyzed combined in one group (Cox regression: t < 24 months, p = 0.009, Hazard ratio: 8.0, 95% CI: 1.7–38.4), with poorer survival solely in low-grade gliomas (LogRank: p = 0.02, Cox regression: p = 0.06, Hazard-Ratio: 8.0, 95% CI: 0.9–66.7), but not in GBM (LogRank: p > 0.05). This might be due to their lower tumor volume at the therapy start. GBM patients with high MPS1 mRNA-expression developed clinical symptoms at an earlier stage. This, however, did not benefit their overall survival, most likely due to the more aggressive tumor growth. Since MPS1 mRNA-expression in gliomas was enhanced with increasing tumor aggressiveness, patients with the worst outcome might benefit best from a treatment directed against MPS1.
Inherited cardiomyopathies are characterized by clinical and genetic heterogeneity that challenge genetic diagnostics. In this study, we examined the diagnostic benefit of exome data compared to targeted gene panel analyses, and we propose new candidate genes. We performed exome sequencing in a cohort of 61 consecutive patients with a diagnosis of cardiomyopathy or primary arrhythmia, and we analyzed the data following a stepwise approach. Overall, in 64% of patients, a variant of interest (VOI) was detected. The detection rate in the main sub-cohort consisting of patients with dilated cardiomyopathy (DCM) was much higher than previously reported (25/36; 69%). The majority of VOIs were found in disease-specific panels, while a further analysis of an extended panel and exome data led to an additional diagnostic yield of 13% and 5%, respectively. Exome data analysis also detected variants in candidate genes whose functional profile suggested a probable pathogenetic role, the strongest candidate being a truncating variant in STK38. In conclusion, although the diagnostic yield of gene panels is acceptable for routine diagnostics, the genetic heterogeneity of cardiomyopathies and the presence of still-unknown causes favor exome sequencing, which enables the detection of interesting phenotype–genotype correlations, as well as the identification of novel candidate genes.
Thalassodendron ciliatum (Forssk.) Den Hartog is a seagrass belonging to the plant family Cymodoceaceae with ubiquitous phytoconstituents and important pharmacological potential, including antioxidant, antiviral, and cytotoxic activities. In this work, a new ergosterol derivative named thalassosterol (1) was isolated from the methanolic extract of T. ciliatum growing in the Red Sea, along with two known first-reported sterols, namely ergosterol (2) and stigmasterol (3), using different chromatographic techniques. The structure of the new compound was established based on 1D and 2D NMR spectroscopy and high-resolution mass spectrometry (HR-MS) and by comparison with the literature data. The new ergosterol derivative showed significant in vitro antiproliferative potential against the human cervical cancer cell line (HeLa) and human breast cancer (MCF-7) cell lines, with IC\(_{50}\) values of 8.12 and 14.24 µM, respectively. In addition, docking studies on the new sterol 1 explained the possible binding interactions with an aromatase enzyme; this inhibition is beneficial in both cervical and breast cancer therapy. A metabolic analysis of the crude extract of T. ciliatum using liquid chromatography combined with high-resolution electrospray ionization mass spectrometry (LC-ESI-HR-MS) revealed the presence of an array of phenolic compounds, sterols and ceramides, as well as di- and triglycerides.
Background:
Triangular fibrocartilage complex (TFCC) lesions commonly cause ulnar-sided wrist pain and instability of the distal radioulnar joint. Due to its triangular shape, discontinuity of the TFCC is oftentimes difficult to visualize in radiological standard planes. Radial multiplanar reconstructions (MPR) may have the potential to simplify diagnosis in CT wrist arthrography. The objective of this study was to assess diagnostic advantages provided by radial MPR over standard planes for TFCC lesions in CT arthrography.
Methods:
One hundred six patients (49 women, 57 men; mean age 44.2 ± 15.8 years) underwent CT imaging after wrist arthrography. Two radiologists (R1, R2) retrospectively analyzed three randomized datasets for each CT arthrography. One set contained axial, coronal and sagittal planes (MPR\(_{Standard}\)), while the other two included an additional radial reconstruction with the rotating center either atop the ulnar styloid (MPR\(_{Styloid}\)) or in the ulnar fovea (MPR\(_{Fovea}\)). Readers evaluated TFCC differentiability and condition. Suspected lesions were categorized using Palmer’s and Atzei’s classification and diagnostic confidence was stated on a fivepoint Likert scale.
Results:
Compared to standard planes, differentiability of the superficial and deep TFCC layer was superior in radial reconstructions (R1/R2; MPR\(_{Fovea}\): p < 0.001; MPRStyloid: p ≤ 0.007). Palmer and Atzei lesions were present in 86.8% (92/106) and 52.8% (56/106) of patients, respectively. Specificity, sensitivity and accuracy for central Palmer lesions did not differ in radial and standard MPR. For peripheral Atzei lesions, sensitivity (MPR\(_{Standard}\) 78.6%/80.4%, MPR\(_{Styloid}\) 94.6%/94.6%, MPR\(_{Fovea}\) 91.1%/89.3%) and accuracy (MPR\(_{Standard}\) 86.8%/86.8%, MPR\(_{Styloid}\) 96.2%/96.2%, MPR\(_{Fovea}\) 94.3%/93.4%) improved with additional styloid-centered (p = 0.004/0.008) and foveacentered (p = 0.039/0.125) reconstructions. No substantial difference was observed between both radial MPR (p = 0.688/0.250). Interrater agreement was almost perfect for each dataset (κ\(_{Standard}\) = 0.876, κ\(_{Styloid}\) = 0.894, κ\(_{Fovea}\) = 0.949). Diagnostic confidence increased with addition of either radial MPR (p < 0.001).
Conclusions:
Ancillary radial planes improve accuracy and diagnostic confidence for detection of peripheral TFCC lesions in CT arthrography of the wrist.
Background
High doses of capsaicin are recommended for the treatment of neuropathic pain. However, low doses evoke mechanical hypersensitivity. Activation of the capsaicin chemosensor transient receptor potential vanilloid 1 (TRPV1) induces neurogenic inflammation. In addition to the release of pro-inflammatory mediators, reactive oxygen species are produced. These highly reactive molecules generate oxidised phospholipids and 4-hydroxynonenal (4-HNE) which then directly activate TRP ankyrin 1 (TRPA1). The apolipoprotein A-I mimetic peptide D-4F neutralises oxidised phospholipids. Here, we asked whether D-4F ameliorates neurogenic hypersensitivity in rodents by targeting reactive oxygen species and 4-HNE in the capsaicin-evoked pain model.
Results
Co-application of D-4F ameliorated capsaicin-induced mechanical hypersensitivity and allodynia as well as persistent heat hypersensitivity measured by Randell–Selitto, von Frey and Hargreaves test, respectively. In addition, mechanical hypersensitivity was blocked after co-injection of D-4F with the reactive oxygen species analogue H2O2 or 4-HNE. In vitro studies on dorsal root ganglion neurons and stably transfected cell lines revealed a TRPA1-dependent inhibition of the calcium influx when agonists were pre-incubated with D-4F. The capsaicin-induced calcium influx in TRPV1-expressing cell lines and dorsal root ganglion neurons sustained in the presence of D-4F.
Conclusions
D-4F is a promising compound to ameliorate TRPA1-dependent hypersensitivity during neurogenic inflammation.
The size of the synaptic subcomponents falls below the limits of visible light microscopy. Despite new developments in advanced microscopy techniques, the resolution of transmission electron microscopy (TEM) remains unsurpassed. The requirements of tissue preservation are very high, and human post mortem material often does not offer adequate quality. However, new reprogramming techniques that generate human neurons in vitro provide samples that can easily fulfill these requirements. The objective of this study was to identify the culture technique with the best ultrastructural preservation in combination with the best embedding and contrasting technique for visualizing neuronal elements. Two induced neural stem cell lines derived from healthy control subjects underwent differentiation either adherent on glass coverslips, embedded in a droplet of highly concentrated Matrigel, or as a compact neurosphere. Afterward, they were fixed using a combination of glutaraldehyde (GA) and paraformaldehyde (PFA) followed by three approaches (standard stain, Ruthenium red stain, high contrast en-bloc stain) using different combinations of membrane enhancing and contrasting steps before ultrathin sectioning and imaging by TEM. The compact free-floating neurospheres exhibited the best ultrastructural preservation. High-contrast en-bloc stain offered particularly sharp staining of membrane structures and the highest quality visualization of neuronal structures. In conclusion, compact neurospheres growing under free-floating conditions in combination with a high contrast en-bloc staining protocol, offer the optimal preservation and contrast with a particular focus on visualizing membrane structures as required for analyzing synaptic structures.
Aims Acute myocardial infarction (MI) is the major cause of chronic heart failure. The activity of blood coagulation factor XIII (FXIIIa) plays an important role in rodents as a healing factor after MI, whereas its role in healing and remodelling processes in humans remains unclear. We prospectively evaluated the relevance of FXIIIa after acute MI as a potential early prognostic marker for adequate healing.
Methods and results This monocentric prospective cohort study investigated cardiac remodelling in patients with ST-elevation MI and followed them up for 1 year. Serum FXIIIa was serially assessed during the first 9 days after MI and after 2, 6, and 12 months. Cardiac magnetic resonance imaging was performed within 4 days after MI (Scan 1), after 7 to 9 days (Scan 2), and after 12 months (Scan 3). The FXIII valine-to-leucine (V34L) single-nucleotide polymorphism rs5985 was genotyped. One hundred forty-six patients were investigated (mean age 58 ± 11 years, 13% women). Median FXIIIa was 118 % (quartiles, 102–132%) and dropped to a trough on the second day after MI: 109%(98–109%; P < 0.001). FXIIIa recovered slowly over time, reaching the baseline level after 2 to 6 months and surpassed baseline levels only after 12 months: 124 % (110–142%). The development of FXIIIa after MI was independent of the genotype. FXIIIa on Day 2 was strongly and inversely associated with the relative size of MI in Scan 1 (Spearman’s ρ = –0.31; P = 0.01) and Scan 3 (ρ = –0.39; P < 0.01) and positively associated with left ventricular ejection fraction: ρ = 0.32 (P < 0.01) and ρ = 0.24 (P = 0.04), respectively.
Conclusions FXIII activity after MI is highly dynamic, exhibiting a significant decline in the early healing period, with reconstitution 6 months later. Depressed FXIIIa early after MI predicted a greater size of MI and lower left ventricular ejection fraction after 1 year. The clinical relevance of these findings awaits to be tested in a randomized trial.
Several oncolytic viruses (OVs) including various human and canine adenoviruses, canine distemper virus, herpes-simplex virus, reovirus, and members of the poxvirus family, such as vaccinia virus and myxoma virus, have been successfully tested for canine cancer therapy in preclinical and clinical settings. The success of the cancer virotherapy is dependent on the ability of oncolytic viruses to overcome the attacks of the host immune system, to preferentially infect and lyse cancer cells, and to initiate tumor-specific immunity. To date, several different strategies have been developed to overcome the antiviral host defense barriers. In our study, we used canine adipose-derived mesenchymal stem cells (cAdMSCs) as a “Trojan horse” for the delivery of oncolytic vaccinia virus Copenhagen strain to achieve maximum oncolysis against canine soft tissue sarcoma (CSTS) tumors. A single systemic administration of vaccinia virus-loaded cAdMSCs was found to be safe and led to the significant reduction and substantial inhibition of tumor growth in a CSTS xenograft mouse model. This is the first example that vaccinia virus-loaded cAdMSCs could serve as a therapeutic agent against CSTS tumors.
Head and neck squamous cell carcinoma (HNSCC) is known to overexpress a variety of receptor tyrosine kinases, such as the HGF receptor Met. Like other malignancies, HNSCC involves a mutual interaction between the tumor cells and surrounding tissues and cells. We hypothesized that activation of HGF/Met signaling in HNSCC influences glucose metabolism and therefore substantially changes the tumor microenvironment. To determine the effect of HGF, we submitted three established HNSCC cell lines to mRNA sequencing. Dynamic changes in glucose metabolism were measured in real time by an extracellular flux analyzer. As expected, the cell lines exhibited different levels of Met and responded differently to HGF stimulation. As confirmed by mRNA sequencing, the level of Met expression was associated with the number of upregulated HGF-dependent genes. Overall, Met stimulation by HGF leads to increased glycolysis, presumably mediated by higher expression of three key enzymes of glycolysis. These effects appear to be stronger in Met\(^{high}\)-expressing HNSCC cells. Collectively, our data support the hypothesized role of HGF/Met signaling in metabolic reprogramming of HNSCC.
Pathophysiological understanding of gait and balance disorders in Parkinson’s disease is insufficient and late recognition of fall risk limits efficacious followup to prevent or delay falls. We show a distinctive reduction of glucose metabolism in the left posterior parietal cortex, with increased metabolic activity in the cerebellum, in parkinsonian patients 6–8 months before their first fall episode. Falls in Parkinson’s disease may arise from altered cortical processing of body spatial orientation, possibly predicted by abnormal cortical metabolism.