Quantitative evidence of suppressed TMEM119 microglial immunohistochemistry in fatal morphine intoxications
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- The aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascularThe aim of this pilot study was to investigate the diagnostic potential of TMEM119 as a useful microglia-specific marker in combination with immunostainings for phagocytic function and infiltrating capacity of monocytes in cases of lethal monosubstance intoxications by morphine (MOR), methamphetamine (METH), and of ethanol-associated death (ETH) respectively. Human brain tissue samples were obtained from forensic autopsies of cases with single substance abuse (MOR, n = 8; ETH, n = 10; METH, n = 9) and then compared to a cohort of cardiovascular fatalities as controls (n = 9). Brain tissue samples of cortex, white matter, and hippocampus were collected and stained immunohistochemically with antibodies against TMEM119, CD68KiM1P, and CCR2. We could document the lowest density of TMEM119-positive cells in MOR deaths with highly significant differences to the control densities in all three regions investigated. In ETH and METH deaths, the expression of TMEM119 was comparable to cell densities in controls. The results indicate that the immunoreaction in brain tissue is different in these groups depending on the drug type used for abuse.…
Autor(en): | Simone Bohnert, Kosmas Georgiades, Camelia-Maria Monoranu, Michael Bohnert, Andreas Büttner, Benjamin Ondruschka |
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URN: | urn:nbn:de:bvb:20-opus-266934 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Rechtsmedizin |
Medizinische Fakultät / Pathologisches Institut | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | International Journal of Legal Medicine |
ISSN: | 1437-1596 |
Erscheinungsjahr: | 2021 |
Band / Jahrgang: | 135 |
Heft / Ausgabe: | 6 |
Seitenangabe: | 2315–2322 |
Originalveröffentlichung / Quelle: | International Journal of Legal Medicine 2021, 135(6):2315–2322. DOI: 10.1007/s00414-021-02699-5 |
DOI: | https://doi.org/10.1007/s00414-021-02699-5 |
PubMed-ID: | https://pubmed.ncbi.nlm.nih.gov/34553260 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | drug abuse; forensic neuropathology; immunohistochemistry; microglia; neuroinflammation; neurotoxicity |
Datum der Freischaltung: | 03.06.2022 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |