Detection of Fusarium Species in Clinical Specimens by Probe-Based Real-Time PCR
Zitieren Sie bitte immer diese URN: urn:nbn:de:bvb:20-opus-193111
- The mold Fusarium is a ubiquitous fungus causing plant, animal and human infections. In humans, Fusarium spp. are the major cause of eye infections in patients wearing contact lenses or after local trauma. Systemic infections by Fusarium spp. mainly occur in immunosuppressed patients and can disseminate throughout the human body. Due to high levels of resistance to antifungals a fast identification of the causative agent is an urgent need. By using a probe-based real-time PCR assay specific for the genus Fusarium we analysed several differentThe mold Fusarium is a ubiquitous fungus causing plant, animal and human infections. In humans, Fusarium spp. are the major cause of eye infections in patients wearing contact lenses or after local trauma. Systemic infections by Fusarium spp. mainly occur in immunosuppressed patients and can disseminate throughout the human body. Due to high levels of resistance to antifungals a fast identification of the causative agent is an urgent need. By using a probe-based real-time PCR assay specific for the genus Fusarium we analysed several different clinical specimens detecting Fusarium spp. commonly found in clinical samples in Germany. Also, a large collection of lung fluid samples of haematological patients was analysed (n = 243). In these, two samples (0.8%) were reproducibly positive, but only one could be confirmed by sequencing. For this case of probable invasive fungal disease (IFD) culture was positive for Fusarium species. Here we describe a rapid, probe-based real-time PCR assay to specifically detect DNA from a broad range of Fusarium species and its application to clinically relevant specimens.…
Autor(en): | Jan Springer, Grit Walther, Volker Rickerts, Axel Hamprecht, Birgit Willinger, Daniel Teschner, Hermann Einsele, Oliver Kurzai, Juergen Loeffler |
---|---|
URN: | urn:nbn:de:bvb:20-opus-193111 |
Dokumentart: | Artikel / Aufsatz in einer Zeitschrift |
Institute der Universität: | Medizinische Fakultät / Institut für Hygiene und Mikrobiologie |
Medizinische Fakultät / Medizinische Klinik und Poliklinik II | |
Sprache der Veröffentlichung: | Englisch |
Titel des übergeordneten Werkes / der Zeitschrift (Englisch): | Journal of Fungi |
ISSN: | 2309-608X |
Erscheinungsjahr: | 2019 |
Band / Jahrgang: | 5 |
Heft / Ausgabe: | 4 |
Seitenangabe: | 105 |
Originalveröffentlichung / Quelle: | Journal of Fungi 2019, 5(4), 105; https://doi.org/10.3390/jof5040105 |
DOI: | https://doi.org/10.3390/jof5040105 |
Allgemeine fachliche Zuordnung (DDC-Klassifikation): | 6 Technik, Medizin, angewandte Wissenschaften / 61 Medizin und Gesundheit / 610 Medizin und Gesundheit |
Freie Schlagwort(e): | Fusarium; bronchoalveolar lavage fluid; fungal molecular diagnostics; probe-based real-time PCR |
Datum der Freischaltung: | 20.10.2020 |
Datum der Erstveröffentlichung: | 12.11.2019 |
Lizenz (Deutsch): | CC BY: Creative-Commons-Lizenz: Namensnennung 4.0 International |